Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 141, Issue 22)

Posted by Seema Grewal, on 4 November 2014

Here are the highlights from the current issue of Development: Meristem maintenance is KNOX so simple Class I KNOX transcription factors, such as SHOOT MERISTEMLESS (STM) and KNAT1, are known ...

In Development this week (Vol. 141, Issue 21)

Posted by Seema Grewal, on 21 October 2014

Here are the highlights from the new issue of Development: Two top tips for angiogenesis The widely accepted model of angiogenic sprouting proposes that a single cell – the ...

In Development this week (Vol. 141, Issue 20)

Posted by Seema Grewal, on 7 October 2014

Here are the highlights from the current issue of Development: Small (molecule) steps to making bone The repair of cartilage and bone following damage remains a clinical challenge. Current ...

In Development this week (Vol. 141, Issue 19)

Posted by Seema Grewal, on 23 September 2014

Here are the highlights from the current issue of Development: Modelling fate decisions in the early mouse embryo In the early embryo, the first fate decision separates the trophectoderm ...

In Development this week (Vol. 141, Issue 18)

Posted by Seema Grewal, on 2 September 2014

Here are the highlights from the current issue of Development: Mcc: a new player in gastrulation The mutated in molorectal cancer (Mcc) gene has been described as a tumour ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010