Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 141, Issue 17)

Posted by Seema Grewal, on 19 August 2014

Here are the highlights from the current issue of Development: HSCs make a Runx1 for it The emergence of haematopoietic stem cells (HSCs) during early mammalian development is crucial ...

In Development this week (Vol. 141, Issue 16)

Posted by Seema Grewal, on 6 August 2014

Here are the highlights from the current issue of Development: PCP signalling is dispensable for neural crest migration The neural crest (NC) is a transient and migratory population of ...

In Development this week (Vol. 141, Issue 15)

Posted by Seema Grewal, on 22 July 2014

Here are the highlights from the current issue of Development: talpid2: a mystery finally solved The chicken talpid2 and talpid3 mutants display a range of developmental phenotypes including craniofacial ...

In Development this week (Vol. 141, Issue 14)

Posted by Seema Grewal, on 8 July 2014

Here are the highlights from the current issue of Development: Prime time for pluripotency Embryonic stem cell (ESC) cultures display a marked heterogeneity in the expression of Nanog, one ...

In Development this week (Vol. 141, Issue 13)

Posted by Seema Grewal, on 24 June 2014

Here are the highlights from the current issue of Development: Eye’s got rhythm In zebrafish, the circadian clock, which is the internal timekeeper that coordinates multiple cellular, physiological and ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010