Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 141, Issue 12)

Posted by Seema Grewal, on 10 June 2014

Here are the highlights from the current issue of Development: Insights into familial dysautonomia Familial dysautonomia (FD) is a germline autosomal recessive disease that is characterized by impaired peripheral ...

In Development this week (Vol. 141, Issue 11)

Posted by Seema Grewal, on 27 May 2014

Here are the highlights from the current issue of Development: Enhanced maturation of induced neurons The ability to reprogram human fibroblasts to neurons in vitro has opened up unprecedented ...

In Development this week (Vol. 141, Issue 10)

Posted by Seema Grewal, on 6 May 2014

Here are the highlights from the current issue of Development: Sara sorts out stem cell asymmetric division Adult stem cells play crucial roles in tissue homeostasis, giving rise to ...

In Development this week (Vol. 141, Issue 9)

Posted by Seema Grewal, on 22 April 2014

Here are the highlights from the new issue of Development: Hemogenic endothelium flexes some muscle Mesoangioblasts (MABs) are progenitor cells of embryonic derivation with mesodermal potential. They have been ...

In Development this week (Vol. 141, Issue 8)

Posted by Seema Grewal, on 8 April 2014

Here are the highlights from the current issue of Development: Spine-tingling new role for Sall4 Wnt, Fgf and retinoic acid signalling play a key role in patterning the posterior ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010