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Displaying posts with the tag: technique [Clear Filter]

Lighting Up the Central Dogma in Development

Posted by on June 19th, 2018

We recently published a manuscript in Cell that describes a method to image transcription factor concentration dynamics in real time, in living embryos, using a nanobody-based protein tag that we call the “LlamaTag.” We were particularly excited about these investigations because this new technology overcomes a major technical obstacle to understanding how gene-expression dynamics are[…]

The people behind the papers – Dan Dickinson

Posted by on September 29th, 2017

Cell polarisation is crucial for normal development and controlled by complex molecular interactions in the cytoplasm and at the membrane. Today we feature a paper recently published in Developmental Cell that describes  a single-cell biochemistry technique and its insights into polarity protein dynamics the developing worm embryo. We caught up with first author Dan Dickinson, who carried out the work as a postdoc[…]

Accelerated Frogs: Developmental Biology meets Particle Physics

Posted by on August 20th, 2014

I previously wrote a post about the development of a 4-D X-Ray Tomography technique for imaging early Xenopus embryos. Frog embryos are opaque due to their yolky composition and this has proved a challenge for traditional optical microscopy of events in the early stages of Xenopus embryo development. However Julian Moosmann, Ralf Hofmann and Jubin[…]

The 1st European BioImage Analysis Symposium, Oct. 7 – 12, 2013 @ Barcelona

Posted by on August 30th, 2013

Image analysis is powerful and essential in modern biology. However, many people working on image analysis might be struggling with following problems: Which tool is appropriate to address my question? Who should I ask my very specific question about image analysis? I want to increase my skills, but I do not know how. I became[…]

Colouring the Mouse Embryo

Posted by on August 13th, 2012

48 anatomical structures of the presented mouse embryo atlas are shown in 3D. Here is the backdrop for our recent paper in Development, “A novel 3D mouse embryo atlas based on micro-CT”.   With the human genome project complete, the sequence and the location of each gene in the genome is understood.  However, the understanding of[…]

Visualizing stem cells at home

Posted by on June 13th, 2011

The Drosophila ovary is stunningly beautiful, and a playground of wonderful biological questions.  Within the germarium alone, developmental biologists can look at asymmetric division, stem cells and their niches, cell migration, and cell specification.  A recent paper in Development describes a technique allowing the in vitro imaging of a fruit fly ovary, and opens the[…]