3 year-POSTDOC + Starting grant (OPENING-SOON BEATRIU de PINOS program) to join our lab on EvoDevoGenomics in BARCELONA

We are seeking candidates to join our lab to study our favorite chordate model Oikopleura dioica, in which we are currently interested in chordate development, specially heart and muscle, and the impact of gene loss on the evolution of gene regulatory signalling networks. Click here for a tour “A day in our lab” posted in The Node

We have also engaged a new EcoEvoDevo line investigating if the developmental mechanisms of marine embryos are ready to respond to climate change, including biotoxins derived from algal blooms. Click here for a tour on this new EcoEvoDevo adventure.

Our approaches include single-cell RNAseq, Embryo microinjection, RNAi, Confocal-Microscopy, Bioinformatics and soon CRISPR

DEADLINE call: February 3rd 2020 (contact for enquiries as soon as possible canestro@ub.edu)

REQUIREMENT: to have defended the PhD within the period January 1st 2012 – December 31st 2017; 2-years of postdoctoral experience; less than 12 months living in Spain the last 3 years.

DURATION:  3 years: starting not later than February 2021 (to be confirmed)

FUNDING: 132.300€ total gross salary for 3 years + 12.000€ research funds

CONTACT: Interested candidates, please send an email to Cristian Cañestro (canestro@ub.edu), including a brief letter of interest, a brief CV, including list of publications with their impact and quartile, and technical skills (specially those related with our approaches) all together in ONE single pdf file.

More info please visit our web: http://goo.gl/0ZaDm0

(1 votes)

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The discipline “Evo-devo” studies the developmental basis of morphological evolution. In the field, some original animal models are emerging as interesting model organisms, enriching the knowledge in the field more and more.

In the DECA team (Développement et évolution du cerveau antérieur, in French) we use an Evo-devo approach to study the developmental mechanisms responsible for brain evolution. Adaptation to new environments brings along changes in brain morphology and function, and these changes are more striking in organisms adapted to extreme environments. A beautiful example that illustrates this topic is the model species used in our lab, the Mexican fish Astyanax mexicanus (teleost). Within the same species there are several river-dwelling populations (surface fish) and other populations adapted to caves (cavefish), living in absolute darkness. Evolution in the caves have led to the total loss of eyes and pigmentation, similarly to many other cave organisms. Since the cavefish embryos initially develop a small eye primordium that then degenerates at larval stages, Astyanax mexicanus has become an attractive model to study the genetic and developmental causes of eyes loss and the mechanisms of sensory compensation.

In our lab some hypotheses have been tested in order to explain the regression of the eyes in cavefish, including modifications of midline signaling centers important for eye induction, or “trade-offs” within the neural plate between the eye field and other neural tissues.

Recently we started to wonder how early in embryogenesis we were able to find differences between the two eco-morphotypes. We decided to compare the process of gastrulation in the two Astyanax morphs, to look at the establishment of the axial signaling centers important for brain development. By comparing systematically the expression of key genes involved in gastrulation we found important heterochronic differences in terms of internalization and migration of the precursors of axial mesoderm, the embryonic organizer. At this point we were very proud of ourselves, because we were able to discriminate between surface fish and cavefish embryos already at the onset of gastrulation, just by looking at in situ hybridizations.

We realized that at the end of gastrulation the anterior axial mesoderm, also called prechordal plate, was different in several aspects, particularly when we looked at the expression of dkk1b (an inhibitor of the WNT pathway). In cavefish embryos, expression of dkk1b occurs in cells that were more dispersed than in surface fish, with lower levels of transcripts and with an earlier off-set of expression, suggesting a globally reduced repression of WNT compared to the surface morphotype. In vertebrates, WNT repression is fundamental for the normal development of the forebrain, including the eyes. Through functional test we showed that modified WNT modulation is indeed implicated in the cavefish eye phenotype.

Next, we reasoned that if at the onset of gastrulation there is already a morphotype-specific embryonic patterning, there might be something different even before this step. Since embryogenesis before gastrulation, including the induction of the organizer, is controlled by determinants present in the oocyte before fertilization occurs, we made the hypothesis that differences from gastrulation onwards are could be due to modified synthesis of maternal determinants. Thus we decided to compare through RNAseq the transcriptomes in embryos just after fertilization, when only maternally-provided mRNAs are present (maternal transcriptomes). When we got the first results we were quite surprised: more than 30% of the maternally-expressed genes were differentially expressed in the two morphs, and around 6600 different loci were de-regulated!     

Then of course we wanted to test the maternal contribution to the cavefish phenotypic evolution. One of the greatest advantages of Astyanax mexicanus as a model is the interfertility between the different populations. In fact, in nature there are some geographic spots where hybridization of cave and river populations occurs nowadays. This unique feature allowed us to test the maternal contribution to a given phenotype by comparing F1 hybrids obtained by reciprocal crosses (hybrids obtained from surface fish eggs versus hybrids obtained from cavefish eggs). If the phenotype under study in the hybrids resembles the phenotype of the female morphotype, then we can say that there is a maternal effect on that trait. The results obtained here were also striking. We found that the patterns of gene expression in hybrids up to the end of gastrulation were exactly the same as those of their maternal morph. These results show first, that the morphotype-specific pattern of gastrulation is determined by maternal factors, and second, that maternal determinants influence embryonic development until stages much later than the maternal-to-zygotic transition. 

After gastrulation, as development continues, all phenotypes analyzed became progressively similar between the reciprocal hybrids, and intermediate between the two pure eco-morphotypes. However, some of these morphotype-specific phenotypes at larval stages (hypothalamic patterning and eye regionalization) showed clear differences in the reciprocal hybrids, with a tendency towards the maternal morphs. This indicates that, when the zygotic genome (in a hybrid condition) takes over the control of development, it tends to homogenize the phenotypes, making less clear the full maternal effect observed at earlier stages.

Our work provided clear evidence that changes in the composition of the oocytes can modify the developmental trajectories, thereby contributing to phenotypic evolution. In this sense, the maternal transcriptome could react under specific environmental conditions, modifying subsequent interdependent developmental events, leading finally to a particular phenotypic outcome, in a way similar to a domino effect.

This work offers a new field for us to dig in. There are some questions we started to ask ourselves, for example could maternal genes be under selective pressure? Which mechanisms could account for their regulation within the ovary? How different is the maternal-to-zygotic transition in Astyanax morphs?

Research done by Jorge Torres-Paz, Julien Leclercq and Sylvie Rétaux at the Paris-Saclay Institute of Neuroscience, CNRS UMR9197, Université Paris-Sud, Université Paris-Saclay, France.

Reference:

2019. Torres-Paz*, J. Leclercq* and S. Rétaux. 2019. Maternally regulated gastrulation as a source of variation contributing to cavefish forebrain evolution. elife 8: e50160. doi: 10.7554/eLife.50160 (* Equal contribution).

(6 votes)

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Translational Immunology

8-9 DECEMBER 2020, GHENT, BELGIUM

Due to the Coronavirus, Translational Immunology and the satellite BIS meeting were postponed from 26-27 March 2020 to the end of the year. We are happy to confirm the new date: 8-9 December 2020.

The aim of this conference is to bridge the translational gap in immunopathology, bringing together clinicians, research scientists and industry partners to discuss prominent advances from the bench to the clinicimmune The different sessions will have a particular focus on precision medicine in general, starting from disease-oriented cases and lessons learned. Innovative and alternative therapeutic strategies in immune-mediated disorders will be presented, besides the regulatory challenges that companies and researchers are dealing with.

Deadlines:

• Abstract submission: 5 October 2020
• Early Bird deadline: 27 October 2020
• Late Registration deadline: 24 November 2020

Speakers:

• Rosa Bacchetta- Stanford Medicine, US
• Dirk Elewaut- VIB-UGent Center for Inflammation Research, BE
• Alain Fischer – Assistance publique – Hôpitaux de Paris, FR
• Martin Guilliams – VIB-UGent Center for Inflammation Research, BE
• Pleun Hombrink – Sanquin, NL
• Bengt Hoepken – Clinical Program Director, UCB Pharma, DE
• Isabelle Huys – KU Leuven, BE
• Christophe Lahorte – National Innovation Office & Scientific-Technical Advice Unit – (Famhp), BE
• Bart Lambrecht – VIB-UGent Center for Inflammation Research, BE
• Antonio Lanzavecchia – Institute for Research in Biomedicine, CH
• Sophie Lucas – de Duve Institute, UCLouvain, BE
• Bénédicte Machiels – FARAH Center, ULiège, BE
• Melanie Matheu – CEO, Founder at Prellis Biologics, Inc., US
• Massimiliano Mazzone – VIB-KU Leuven Center for Cancer Biology, BE
• Kathy McCoy – University of Calgary, CA
• Eoin McKinney – University of Cambridge, UK
• Fiona Powrie – Kennedy Institute of Rheumatology, University of Oxford, UK
• Federica Sallusto – Institute for Research in Biomedicine, CH
• Georg Schett – Friedrich-Alexander University Erlangen-Nürnberg, DE
• Marvin van Luijn – Erasmus MC, University Medical Center Rotterdam MS Center ErasMS, NL

Conference website

Location: De Bijloke, Jozef Kluyskensstraat 2, 9000 Gent

Hashtag: #TransImmu20

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This obituary by Frank Jülicher was recently published in Development

Suzanne Eaton, Professor at the Technical University Dresden and Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, tragically died on 2 July 2019. Suzanne was a remarkable person, both as a scientist and as a human being. Having worked closely with Suzanne for many years, I remember here some of her key scientific contributions.

Suzanne truly loved science and was driven by a deep curiosity for nature. She was an exceptional scientist with a taste for profound and fundamental problems in biology, and she embraced novel, original and interdisciplinary approaches. Indeed, Suzanne was a pioneer in quantitative approaches to tissue morphogenesis and a leader in the field, bridging scales from cell biology to tissue dynamics. With her fast wit and broad knowledge, she inspired colleagues, co-workers and students alike. Working with her was always a joyful experience, playful and deeply enlightening. Suzanne was also a sophisticated piano player and she had a black belt in Taekwondo. As one of Suzanne’s close collaborators over the past 15 years, it has been a true privilege for me to have interacted with her on a number of exciting problems and to have had weekly joint group meetings with a broad and interdisciplinary spirit.

Born in Oakland, California, Suzanne did her PhD at UCLA in the group of Kathryn Calame. During her PhD, Suzanne worked on the transcriptional regulation of immunoglobulin heavy chain genes (Eaton and Calame, 1987). She then moved to the lab of Tom Kornberg at UCSF, where she worked on the fruit fly Drosophila melanogaster and began to investigate fundamental aspects of Hedgehog signalling. Suzanne discovered that Hedgehog is a membrane-associated signalling molecule and that its expression is confined to posterior compartment cells (Tabata et al., 1992). In 1993, Suzanne moved to EMBL in Heidelberg to the lab of Kai Simons. There, she investigated planar cell polarity in epithelial tissues and revealed how Rho family GTPases play a role in polarizing the actin cytoskeleton and regulating cell shape changes in the fly wing epithelium (Eaton et al., 1995).

In 2000, Suzanne moved to Dresden to help set up the newly founded Max Planck Institute of Molecular Cell Biology and Genetics. In this environment, her research flourished as she became a leader in a new field that brought together cell and developmental biology. She was curious about how morphogens such as Hedgehog and Wnt/Wingless that spread in a tissue over distances could be found tightly associated with membranes. She discovered that these morphogens travel while associated with membranous particles, which move between cells and act as vehicles for morphogen transport (Greco et al., 2001). She further established that these membranous particles are lipoprotein particles (Panáková et al. 2005).

Suzanne then became fascinated by the geometry of cell packing, which appears to be random but at the same time exhibits different types of order and structural features. She thus investigated the mechanisms that govern cell neighbour numbers and the establishment of hexagonal packing in epithelia, revealing a role for planar cell polarity proteins in this process (Classen et al., 2005). This interest in cell packing geometries and cell polarity patterns triggered stimulating discussions about the role of forces and cell mechanics in morphogenesis, which in turn brought about a fruitful, long-lasting and inspiring collaboration between our research groups. A first step in this collaboration was the development of vertex models that capture the forces that define cell shapes. By comparing experiment and theory, key parameters that characterize biophysical properties of cells could be inferred and a general mechanism for the emergence of hexagonal cell packing was identified (Farhadifar et al., 2007). Live imaging over extended periods of time then permitted Suzanne to quantify cell movements, cell flow patterns and dynamic patterns of planar cell polarity over time during Drosophila pupal development. These observations revealed that planar cell polarity patterns in the tissue are reoriented by cell flow and tissue shear. This provided new insights into the role of cell flow in shaping patterns during morphogenesis (Aigouy et al., 2010). Looking at cell polarity patterns at early and late time points in the wing imaginal disc revealed how planar cell polarity patterns that are aligned over large distances could emerge in a tissue. At early time points, cell polarity is oriented in random directions. Groups of cells then locally align their polarity with the help of signals at compartment boundaries, while the tissue is still small. This aligned pattern is then extended over larger scales by tissue growth (Sagner et al., 2012).

Another key question that Suzanne addressed is how the fly wing takes on its final shape. Interestingly, mutants of the protein Dumpy, which links the tissue to the extracellular matrix, exhibit strongly misformed wings. Live imaging of wing development in dumpy mutants revealed that mechanical attachments at the tissue margin have a direct and strong influence on final tissue shape. It was shown that the wing is shaped inside the pupa by an active mechanical process that involves tissue contraction and tissue flow, which depend on patterns of mechanical boundary attachments (Etournay et al., 2015).

Suzanne’s discovery that signalling molecules such as Hedgehog are transported over distances by lipoprotein particles, which are also carriers of lipids, led her to bridge the field of morphogenesis with that of metabolism. She therefore started a research programme to investigate metabolic regulation and the complex interplay between metabolism, growth and development. She discovered that Drosophila lipoproteins generated in the fat body (a structure playing a role similar to that of the liver) provide signals about the nutritional status of the organism that are sent to the brain where they accumulate. In the brain, in turn, insulin-like peptides are secreted to regulate insulin signalling. Surprisingly, lipoproteins accumulate in the brain if the fly is on a yeast diet but not when it is on a plant-based diet. In this case, flies develop and grow more slowly, and live longer, as a consequence of different insulin signalling despite the calorimetric food content being the same. This work thus provided fascinating insights into the regulation of metabolism under varying diets (Brankatschk et al., 2014).

What could be the adaptive roles of different diets? In a very beautiful paper, Suzanne and her team investigated the effects of plant food compared with yeast food on the survival of Drosophila larvae and adults at different temperatures (Brankatschk et al., 2018). They showed that whereas flies growing in warm temperatures prefer yeast food, they prefer plant food when they are maintained at cold temperatures. Furthermore, flies on a plant diet can survive cold temperatures at which flies that are kept on a yeast diet will die. Thus, the choice of the appropriate food is important for survival during winter periods in temperate climates. A key difference between plant and yeast food is the ability of plants to produce polyunsaturated fatty acids. Suzanne and colleagues showed that the difference in diet leads to different lipid composition of membranes. Membranes of larvae on a plant diet maintain fluid properties and exhibit disordered membrane organization at lower temperatures, suggesting that these membrane biophysical properties are modulated by nutrition and are important for survival in the cold.

Remembering her contributions reveals the immense breadth of Suzanne’s work and her ability to bridge different fields and disciplines when addressing important questions in biology. Her work is characterized by very original and deep studies using strong quantitative approaches. She always looked at fundamental problems from new angles and thus made important and surprising discoveries. Her loss leaves a gaping void, and her sharp intellect and warm personality are missed tremendously.

Suzanne Eaton has also been remembered in Nature Cell Biology, Cell, eLife, the MPI-CBG and EMBL.

(3 votes)

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The Blythe Lab at Northwestern University seeks to recruit a motivated postdoctoral fellow to investigate chromatin dynamics in early Drosophila embryogenesis.

Research in the Blythe Lab focuses on a critical period of embryogenesis termed the maternal-to-zygotic transition (MZT). During this time, embryos establish an initial ‘ground state’ of chromatin structure that defines the initial cis-regulatory landscape underlying the embryo’s first cell fate decisions. We are interested both in how the initial state is established and how this constrains the interpretation of developmental cues during pattern formation. To study this question, we apply a combination of genetic, genomic, and quantitative imaging approaches to understand the mechanisms that shape the embryonic chromatin landscape.

Projects are available in three major areas at the interface of developmental biology, epigenetics, and systems biology: 1) Temporal control of zygotic genome activation; 2) Patterning-dependent chromatin remodeling and gene regulatory network function; 3) Conflicts between DNA replication and transcription.

We are particularly interested in candidates with experience in genomic approaches (RNA-, ChIP-, or ATAC-seq), analysis of genomic data, quantitative confocal microscopy, biophysical approaches, and/or Drosophila genetics. The position available immediately.

Selected references:

Soluri et al, Biorxiv 2019 (https://doi.org/10.1101/852707)

Hannon et al, eLife 2017 (https://doi.org/10.7554/eLife.28275)

Blythe and Wieschaus, eLife 2016 (https://doi.org/10.7554/eLife.20148)

Blythe and Wieschaus, Cell 2015 (https://doi.org/10.1016/j.cell.2015.01.050)

Interested applicants should forward a cover letter, CV, and list of three references to:

shelby.blythe@northwestern.edu

Shelby A. Blythe, Ph.D.

Assistant Professor

Department of Molecular Biosciences

Northwestern University

Evanston, IL 60202

United States

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We have two fully funded PhD positions in the Wheeler lab at University of East Anglia, Norwich, UK.

NEUcrest is an Innovative Training Network (ITN) project, funded by the European Union Horizon 2020 Programme. The neural crest (NC) is an essential stem cell population of the vertebrate embryos that gives rise to various tissues in the body such as the cranial facial cartilage, peripheral nervous system and the Adrenal Medulla. NEUcrest focuses on integrating academic, clinical and industrial research for a better understanding of neural crest development and neural crest related diseases called Neurocristopathies. These pathologies are a major group of congenital diseases in human, and a heavy societal concern. The NEUcrest network comprises 20 partners in academia, industry and hospitals from seven European countries, gathered in a synergistic effort to advance knowledge and outreach about these diseases.

1. Modelling Neurocristopathies in Xenopus, mechanisms and drug screening

2. Micro RNA regulation of neural crest development

Please follow these links for more information:

1. https://euraxess.ec.europa.eu/jobs/447869
2. https://www.euraxess.fo/jobs/461086

Or contact Dr. Grant Wheeler for more information: Grant.Wheeler@uea.ac.uk

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In this episode we’re bringing you highlights from the Society’s Centenary Conference, held up in Edinburgh last month.

We’ve got stories of sneaky sheep, substandard racing stallions, the Vikings of the Scottish Isles and a ceilidh with a scientific spin. Plus, news from the front lines of the sperm wars.

Listen now through the player below, or subscribe from Apple podcasts/iTunes, Spotify and all good podcast apps to make sure you get the latest episodes and catch up on our back catalogue.

Full transcript and show notes available from GeneticsUnzipped.com

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In this episode from our centenary series exploring 100 ideas in genetics, we’re uprooting the tree of life – asking whether we should believe our eyes or our sequencing machines when it comes to deciding what makes a species.

Plus, the greatest comebacks of all time – we look at the science of de-extinction and find out whether Jurassic Park could ever become a reality.

Listen now through the player below, or subscribe from Apple podcasts/iTunes, Spotify and all good podcast apps to make sure you get the latest episodes and catch up on our back catalogue.

Full transcript and show notes available from GeneticsUnzipped.com

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By Ashish Deshwar and Nicole Edwards

Two weeks ago, we had the opportunity to attend the Company of Biologists Workshop, “Understanding Birth Defects in the Genomic Age”. This workshop brought together a diverse collection of basic developmental biologists, human geneticists and clinicians to discuss the current challenges and opportunities in the field of birth defects research. We can almost guarantee you that none of the groups of attendees would normally overlap at any other meeting. And yet, what quickly became apparent was that there was so much in common between everyone in attendance.

The setting for the meeting was stunning. Set at Wiston House in the English countryside not too far from Brighton, history oozed from every corner of the building. From the delicious catering, to the beautiful (but rainy) countryside walk, to the very interesting talk on the history of the house, this venue provided an epic background for what would be an extremely stimulating three and a half days.

The meeting was unique right from the start. It was definitely the smallest meeting we had ever attended. There were only about 33 people total, and we each had the opportunity to introduce ourselves to the group on the first day. We were asked to give a few slides introducing ourselves as well as a “problem” (a topic that we would like help with) and “solution” (something that we were good at/could use to help others). Right away this encouraged discussion and it became quickly apparent how each of our skillsets would be able to help not just each others’ research, but contribute a unique perspective to the group.

In addition to traditional scientific talks, we had the opportunity to sit down and talk amongst the group about some of the issues which we would like to address. Collectively, birth defects are the number one cause of infant mortality in the USA, but it is sometimes challenging to justify studying individual rare developmental defects. Many of our discussions centred around how to increase public awareness of our research and to emphasize the huge impact of return of results to families – even if a genetic diagnosis doesn’t lead to a treatment, patients and families can be hugely comforted to know more about the underlying cause. John Wallingford gave a stirring talk about the disturbing history of society’s (including the medical profession’s) poor treatment of individuals with congenital anomalies. There was unanimous agreement amongst the group that we needed a new term for birth defects that was both more sensitive and more inclusive. There was much discussion, but the group was not able to come to a consensus as to a better term moving forward. One of our favourite suggestions was that perhaps we needed to create a new word entirely!

One of the goals of the workshop was to generate actual concrete steps to address the above issues. Working groups were established to draft a white paper, review articles, social media campaigns, and other methods to increase the awareness of this very important field of research. One of they key action items became bringing together a similar group of people again to build on the ideas started at this workshop.  The Company of Biologists is already planning a meeting on developmental disorders for 2021; hopefully this will provide another opportunity for a meeting of minds to continue to forward the cause.

As two early career researchers funded by the Company of Biologists to attend the workshop, we gained great insight into the diverse approaches being used to study birth defects research. In addition, it provided a unique opportunity to discuss and learn about larger scope projects such as impacting public awareness and advocating for increased funding for a particular area of research.

At the conclusion of the meeting everyone left inspired and ready to act. We are looking forward to 2021!

Find out more about The Company of Biologists’ Workshops, including our 2020 schedule and details of how to apply for funded places, here:

biologists.com/workshops

(2 votes)

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Welcome to our monthly trawl for developmental biology (and related) preprints. 

This month we found preprints detailing extensive mouse and fly knockout resources, exploring bacterial influences on development, and investigating mechanics in vivo and in silico. They were hosted on bioRxiv and arXiv. Let us know if we missed anything. Use these links to get to the section you want:

Developmental biology

| Patterning & signalling

| Morphogenesis & mechanics

| Genes & genomes

| Stem cells, regeneration & disease modelling

| Plant development

Evo-devo & evo

Cell biology

Modelling

Tools & resources

Research practice & education

Why not…

Developmental biology

| Patterning & signalling

Equilin does not affect thyroid hormone signaling in the developing Xenopus laevis tadpole brain
Robert G. Bass III, Zahabiya Husain, Lara Dahora, Christopher K. Thompson

Atypical Protein Kinase C iota (PKCλ/ι) Ensures Mammalian Development by Establishing the Maternal-Fetal Exchange Interface
Bhaswati Bhattacharya, Pratik Home, Avishek Ganguly, Soma Ray, Ananya Ghosh, Rashedul Islam, Valerie French, Courtney Marsh, Sumedha Gunewardena, Hiroaki Okae, Takahiro Arima, Soumen Paul

Transient Nodal signalling in left precursors coordinates opposed asymmetries shaping the heart loop
Audrey Desgrange, Jean-François Le Garrec, Ségolène Bernheim, Tobias Holm Bønnelykke, Sigolène M. Meilhac

Arkadia via SNON enables NODAL-SMAD2/3 signaling effectors to transcribe different genes depending on their levels
Jonathon M. Carthy, Marilia Ioannou, Vasso Episkopou

Combined modulation of SHH and FGF signaling is crucial for maintenance of the neocortical progenitor specification program
Odessa R. Yabut, Hui-Xuan Ng, Keejung Yoon, Hector G. Gomez, Jessica C. Arela, Samuel J. Pleasure

Synaptogenic activity of the axon guidance molecule Robo2 is critical for hippocampal circuit function
Heike Blockus, Sebastian V. Rolotti, Miklos Szoboszlay, Tiffany Ming, Anna Schroeder, Kristel M. Vennekens, Phinikoula Katsamba, Fabiana Bahna, Seetha Mannepalli, Goran Ahlsen, Barry Honig, Lawrence Shapiro, Joris de Wit, Attila Losonczy, Franck Polleux

Fate of developmental mechanisms of myocardial plasticity in the postnatal heart
Konstantinos E. Hatzistergos, Michael A. Durante, Krystalenia Valasaki, J. William Harbour, Joshua M. Hare

Tfam knockdown results in reduction of mtDNA copy number, OXPHOS deficiency and abnormalities in zebrafish embryos
Auke BC Otten, Rick Kamps, Patrick Lindsey, Mike Gerards, Hélène Pendeville-Samain, Marc Muller, Florence HJ van Tienen, Hubert JM Smeets

DRP1-mediated regulation of mitochondrial dynamics determines the apoptotic response upon embryonic differentiation
Barbara Pernaute, Juan Miguel Sánchez Nieto, Salvador Pérez-Montero, Aida di Gregorio, Ana Lima, Katerina Lawlor, Sarah Bowling, Gianmaria Liccardi, Alejandra Tomás, Pascal Meier, Guy A. Rutter, Ivana Barbaric, Tristan A. Rodríguez

NLRP7 Plays A Functional Role in Regulating BMP4 Signaling During Differentiation of Patient-Derived Trophoblasts
Aybuke Garipcan, Burcu Ozcimen, Ilke Suder, Volkan Ulker, Tamer Tevfik Onder, Nesrin Ozoren

Trophoblast paracrine signaling regulates placental hematoendothelial niche
Pratik Home, Ananya Ghosh, Ram Parikshan Kumar, Avishek Ganguly, Bhaswati Bhattacharya, Md. Rashedul Islam, Soma Ray, Sumedha Gunewardena, Soumen Paul

Chronic cortisol exposure in early development leads to neuroendocrine dysregulation in adulthood
Ellen I. Hartig, Shusen Zhu, Benjamin L. King, James A. Coffman

S1P-S1PR1 activity controls VEGF-A signaling during lymphatic vessel development
AM Golding-Ochsenbein, S Vidal, B Wilmering Wetter, C Guibourdenche, C Beerli, L Chang, S Leonhard, N Holway, K Seuwen, G Jurisic

Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
Lisandra Vila Ellis, Margo P Cain, Vera Hutchison, Per Flodby, Edward D Crandall, Zea Borok, Bin Zhou, Edwin J Ostrin, Joshua D Wythe, Jichao Chen

Chemokine receptors ACKR2 and CCR1 coordinate macrophage dynamics and mammary gland development
Gillian J Wilson, Ayumi Fukuoka, Samantha R Love, Jiwon Kim, Marieke Pingen, Alan J Hayes, Gerard J Graham

Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin
Alin Vonica, Neha Bhat, Keith Phan, Jinbai Guo, Lăcrimioara Iancu, Jessica A. Weber, Amir Karger, John W. Cain, Etienne C. E. Wang, Gina M. DeStefano, Anne H. O’Donnell-Luria, Angela M. Christiano, Bruce Riley, Samantha J. Butler, Victor Luria

Wnt Regulation: Exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex
Kristina N. Schaefer, Mira Pronobis, Clara E. Williams, Shiping Zhang, Lauren Bauer, Dennis Goldfarb, Feng Yan, M. Ben Major, Mark Peifer

Modulation of promoter occupancy dictates the transcriptional response to graded BMP signalling levels in the Drosophila embryo
Caroline Hoppe, Jonathan Bowles, Thomas G Minchington, Catherine Sutcliffe, Priyanka Upadhyai, Magnus Rattray, Hilary L Ashe

Feedback control of morphogen gradient scale
Yilun Zhu, Yuchi Qiu, Weitao Chen, Qing Nie, Arthur D. Lander

Transneuronal interactions facilitate axonal compartment formation
Bavat Bornstein, Idan Alyagor, Victoria Berkun, Hagar Meltzer, Fabienne Reh, Hadas Keren-Shaul, Eyal David, Thomas Riemensperger, Oren Schuldiner

The assembly of the Drosophila mushroom body circuit and its regulation by Semaphorin 1a
Chen-Han Lin, Suewei Lin

The Golgi Glycoprotein MGAT4D is an Intrinsic Protector of Testicular Germ Cells From Mild Heat Stress
Ayodele Akintayo, Meng Liang, Boris Bartholdy, Frank Batista, Jennifer Aguilan, Jillian Prendergast, Subha Sundaram, Pamela Stanley

A cell fate switch in the C. elegans seam cell lineage occurs through modulation of the Wnt asymmetry pathway in response to temperature increase
Mark Hintze, Sneha L. Koneru, Sophie P.R. Gilbert, Dimitris Katsanos, Michalis Barkoulas

| Morphogenesis & mechanics

Cell lineage-dependent chiral actomyosin flows drive cellular rearrangements in early development
Lokesh Pimpale, Teije C. Middelkoop, Alexander Mietke, Stephan W. Grill

Brownian-like deviation of neighboring cells in the early embryogenesis of the zebrafish
Juan Raphael Diaz Simoes (LPMC), Denis Grebenkov (LPMC), Paul Bourgine, Nadine Peyriéras

Caenorhabditis elegans PIEZO Channel Coordinates Multiple Reproductive Tissues to Govern Ovulation
Xiaofei Bai, Jeff Bouffard, Avery Lord, Katherine Brugman, Paul W. Sternberg, Erin J. Cram, Andy Golden

Redox signaling modulates Rho activity and tissue contractility in the C. elegans spermatheca
Charlotte A. Kelley, Sasha De Henau, Liam Bell, Tobias B. Dansen, Erin J. Cram

The Reissner Fiber is Highly Dynamic in vivo and Controls Morphogenesis of the Spine
Benjamin Troutwine, Paul Gontarz, Ryoko Minowa, Adrian Monstad-Rios, Mia J. Konjikusic, Diane S. Sepich, Ronald Y. Kwon, Lilianna Solnica-Krezel, Ryan S. Gray

Chemokine-biased robust self-organizing polarization of migrating cells in vivo
Adan Olguin-Olguin, Anne Aalto, Benoit Maugis, Michal Reichman-Fried, Erez Raz

Cell-fate plasticity, adhesion and cell sorting complementarily establish a sharp midbrain-hindbrain boundary
Gokul Kesavan, Stefan Hans, Michael Brand

Cytokinesis and post-abscission midbody remnants are regulated during mammalian brain development
Katrina C. McNeely, Noelle D. Dwyer

Alpha 6 Integrins Regulate the Expression of Laminin-511 and CXCR4 to Promote Endothelial Tubular Morphogenesis
Hao Xu, Kevin Pumiglia, Susan E. LaFlamme

Mechanical plasticity of the ECM directs invasive branching morphogenesis in human mammary gland organoids
B. Buchmann, L.K. Meixner, P. Fernandez, F.P. Hutterer, M.K. Raich, C.H. Scheel, A.R. Bausch

Asymmetric Hapln1a drives regionalised cardiac ECM expansion and promotes heart morphogenesis during zebrafish development
Christopher J Derrick, Juliana Sánchez-Posada, Farah Hussein, Federico Tessadori, Eric JG Pollitt, Aaron M Savage, Robert N Wilkinson, Timothy J Chico, Fredericus JM van Eeden, Jeroen Bakkers, Emily S Noël

Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration
Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé

Microtubule-dependent protein trafficking promotes apical constriction during tissue invagination
Thao Phuong Le, SeYeon Chung

Different principles govern different scales of brain folding
Arka N Mallela, Hansen Deng, Alan Bush, Ezequiel Goldschmidt

Primary cilia are present on endothelial cells of the hyaloid vasculature but are not required for the development of the blood-retinal barrier
Lana M. Pollock, Brian Perkins, Bela Anand-Apte

The lhfpl5 ohnologs lhfpl5a and lhfpl5b are required for mechanotransduction in distinct populations of sensory hair cells in zebrafish.
Timothy Erickson, Itallia V Pacentine, Alexandra Venuto, Rachel Clemens, Teresa Nicolson

The Heparan Sulfate Proteoglycan Syndecan-1 Influences Local Bone Cell Communication via the RANKL/OPG Axis
Melanie Timmen, Heriburg Hidding, Martin Götte, Thaqif El Khassawna, Daniel Kronenberg, Richard Stange

Syndecan-3 enhances anabolic bone formation through WNT signalling
Francesca Manuela Johnson de Sousa Brito, Andrew Butcher, Addolorata Pisconti, Blandine Poulet, Amanda Prior, Gemma Charlesworth, Catherine Sperinck, Michele Scotto di Mase, George Bou-Gharios, Robert Jurgen van ’t Hof, Anna Daroszewska

Dynamic expression of MMP28 during cranial morphogenesis
Nadege Gouignard, Eric Theveneau, Jean-Pierre Saint-Jeannet

Pits and CtBP control tissue growth in Drosophila melanogaster with the Hippo pathway transcription repressor, Tgi
Joseph H.A. Vissers, Lucas G. Dent, Colin House, Shu Kondo, Kieran F. Harvey

Astral microtubule crosslinking by Feo safeguards uniform nuclear distribution in the Drosophila syncytium
Ojas Deshpande, Jorge de-Carvalho, Diana M Vieira, Ivo Andreas Telley

Microtubule-dependent protein trafficking promotes apical constriction during tissue invagination
Thao Phuong Le, SeYeon Chung

The engulfment receptor Draper organizes the postsynaptic spectrin cytoskeleton into corrals containing synaptic proteins and promotes synaptic renewal
Simon Wang, Mannan Wang, Hae-yoon Kim, Nicole Yoo, Matias Raski, Claire Shih, Clare Zheng, Kevin Tran, Wade Parkhouse, Charles Krieger, Nicholas Harden

Regionalized tissue fluidization by an actomyosin cable is required for epithelial gap closure during insect gastrulation
A. Jain, V. Ulman, A. Mukherjee, M. Prakash, L. Pimpale, S. Muenster, R. Haase, K.A. Panfilio, F. Jug, S.W. Grill, P. Tomancak, A. Pavlopoulos

| Genes & genomes

Natural cryptic epigenetic variation in an embryonic gene regulatory network
Chee Kiang Ewe, Yamila N. Torres Cleuren, Russell G. Snell, Joel H. Rothman

mir-71 mediates age-dependent opposing contributions of the stress activated kinase KGB-1 in Caenorhabditis elegans
Cyrus Ruediger, Michael Shapira

DREAM Interrupted: Severing MuvB from DREAM’s pocket protein in Caenorhabditis elegans impairs gene repression but not DREAM chromatin assembly
Paul D. Goetsch, Susan Strome

Loss of histone H3.3 results in DNA replication defects and altered origin dynamics in C. elegans
Maude Strobino, Joanna M. Wenda, Florian A. Steiner

A Long Lost Key Opens an Ancient Lock: Drosophila Myb Causes a Synthetic Multivulval Phenotype in Nematodes
Paul J. Vorster, Paul Goetsch, Tilini U. Wijeratne, Keelan Z. Guiley, Laura Andrejka, Sarvind Tripathi, Braden J. Larson, Seth M. Rubin, Susan Strome, Joseph S. Lipsick

Odd-paired is a late-acting pioneer factor coordinating with Zelda to broadly regulate gene expression in early embryos
Theodora Koromila, Fan Gao, Yasuno Iwasaki, Peng He, Lior S Pachter, John Peter Gergen, Angelike Stathopoulos

Zygotic pioneer factor activity of Odd-paired/Zic is necessary for establishing the Drosophila Segmentation Network
Isabella V. Soluri, Lauren M. Zumerling, Omar A. Payan Parra, Eleanor G. Clark, Shelby A. Blythe

Topology-driven analysis of protein-protein interaction networks detects functional genetic modules regulating reproductive capacity
Tarun Kumar, Leo Blondel, Cassandra G. Extavour

The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm
Anne Ramat, Maria-Rosa Garcia-Silva, Camille Jahan, Rima Nait-Saidi, Jeremy Dufourt, Celine Garret, Aymeric Chartier, Julie Cremaschi, Vipul Patel, Mathilde Decourcelle, Amandine Bastide, Francois Juge, Martine Simonelig

Drosophila models of pathogenic copy-number variant genes show global and non-neuronal defects during development
Tanzeen Yusuff, Matthew Jensen, Sneha Yennawar, Lucilla Pizzo, Siddharth Karthikeyan, Dagny J. Gould, Avik Sarker, Yurika Matsui, Janani Iyer, Zhi-Chun Lai, Santhosh Girirajan

Neuronal upregulation of Prospero protein is driven by alternative mRNA polyadenylation and Syncrip-mediated mRNA stabilisation
Tamsin J. Samuels, Yoav Arava, Aino I. Järvelin, Francesca Robertson, Jeffrey Y. Lee, Lu Yang, Ching-Po Yang, Tzumin Lee, David Ish-Horowicz, Ilan Davis

Role of a versatile peptide motif in controlling Hox nuclear export and autophagy in the Drosophila fat body
Marilyne Duffraisse, Rachel Paul, Bruno Hudry, Julie Carnesecchi, Agnes Banretti, Jonathan Reboulet, Leiore Ajuria, Ingrid Lohmann, Samir Merabet

Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development
Alicia Estacio-Gómez, Amira Hassan, Emma Walmsley, Lily Lee, Tony D. Southall

High-Resolution Single-Cell Models of Ensemble Chromatin Structures during Drosophila Embryogenesis from Population Hi-C
Qiu Sun, Alan Perez-Rathke, Daniel M. Czajkowsky, Zhifeng Shao, Jie Liang

Sex chromosome pairing mediated by euchromatic homology in Drosophila male meiosis
Christopher A. Hylton, Katie Hansen, Andrew Bourgeois, John E. Tomkiel

dP75 safeguards oogenesis by preventing H3K9me2 spreading
Kun Dou, Yanchao Liu, Yingpei Zhang, Chenhui Wang, Ying Huang, ZZ Zhao Zhang

bsAS, an antisense long non-coding RNA, controls cell fate through regulation of blistered/DSRF isoform expression
Sílvia Pérez-Lluch, Alessandra Breschi, Cecilia C. Klein, Marina Ruiz-Romero, Amaya Abad, Emilio Palumbo, Lyazzat Bekish, Carme Arnan, Roderic Guigó

DCP2 plays multiple roles during Drosophila development – possible case of moonlighting?
Rohit Kunar, Jagat K Roy

Astrocyte-like glia-specific gene deathstar is crucial for normal development, adult locomotion and lifespan of male Drosophila
Hadi Najafi, Kyle Wong, Woo Jae Kim

Characterisation of protein isoforms encoded by the Drosophila Glycogen Synthase Kinase 3 gene shaggy
Dagmara Korona, Daniel J. H. Nightingale, Bertrand Fabre, Michael Nelson, Bettina Fischer, Glynnis Johnson, Jonathan Lees, Simon Hubbard, Kathryn Lilley, Steven Russell

Developmentally-orchestrated mitochondrial processes prime the selective inheritance against harmful mitochondrial DNA mutations
Zhe Chen, Zong-Heng Wang, Guofeng Zhang, Christopher K. E. Bleck, Dillon J. Chung, Grey Madison, Eric Lindberg, Christian Combs, Robert S. Balaban, Hong Xu

Maternal- and Somatic-type snoRNA Expression and Processing in Zebrafish Development
Johanna F.B. Pagano, Mauro D. Locati, Wim A Ensik, Marina van Olst, Selina van Leeuwen, Wim C. De Leeuw, Ulrike Nehrdich, Herman P Spaink, Han Rauwerda, Martijs J. Jonker, Rob J. Dekker, Timo M Breit

An alternative spliceosome defined by distinct snRNAs in early zebrafish embryogenesis
Johanna F.B. Pagano, Rob J Dekker, Wim A. Ensink, Marina van Olst, Alex Bos, Selina van Leeuwen, Wim C. de Leeuw, Ulrike Nehrdich, Herman P. Spaink, Han Rauwerda, Martijs J. Jonker, Timo M. Breit

Sox2 and Sox3 are essential for development and regeneration of the zebrafish lateral line
Cristian A. Undurraga, Yunzi Gou, Pablo C. Sandoval, Viviana A. Nuñez, Miguel L. Allende, Bruce B. Riley, Pedro P. Hernández, Andres F. Sarrazin

The conserved and divergent roles of Prdm3 and Prdm16 in zebrafish and mouse craniofacial development
Lomeli Shull, Rwik Sen, Johannes Menzel, Kristin Bruk Artinger

Lin28a/let-7 Pathway Modulates the Hox Code via Polycomb Regulation during Axial Patterning in Vertebrates
Tempei Sato, Kensuke Kataoka, Yoshiaki Ito, Shigetoshi Yokoyama, Masafumi Inui, Masaki Mori, Satoru Takahashi, Keiichi Akita, Shuji Takada, Hiroe Ueno-Kudoh, Hiroshi Asahara

A TBX5 dosage-sensitive gene regulatory network for human congenital heart disease
Irfan S. Kathiriya, Kavitha S. Rao, Giovanni Iacono, W. Patrick Devine, Swetansu K. Hota, Michael H. Lai, Bayardo I. Garay, Reuben Thomas, Andrew P. Blair, Henry Z. Gong, Lauren K. Wasson, Piyush Goyal, Tatyana Sukonnik, Gunes A. Akgun, Laure D. Bernard, Brynn N. Akerberg, Fei Gu, Kai Li, William T. Pu, Joshua M. Stuart, Christine E. Seidman, J. G. Seidman, Holger Heyn, Benoit G. Bruneau

Dppa2/4 target chromatin bivalency enabling multi-lineage commitment
Mélanie A. Eckersley-Maslin, Aled Parry, Marloes Blotenburg, Christel Krueger, Valar Nila Roamio Franklin, Stephen J. Clark, Clive S. D’Santos, Wolf Reik

Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells
Sruti Patoori, Nathalie Jean-Charles, Ariana Gopal, Sacha Sulaiman, Sneha Gopal, Brian Wang, Benjamin Souferi, Mark M. Emerson

Transcription factors protect from DNA re-methylation during reprograming of primordial germ cells and pre-implantation embryos
Isaac Kremsky, Victor G. Corces

Suppression of DSB Formation by Polβ in Active DNA Demethylation is Required for Postnatal Hippocampal Development
Akiko Uyeda, Kohei Onishi, Teruyoshi Hirayama, Satoko Hattori, Tsuyoshi Miyakawa, Takeshi Yagi, Nobuhiko Yamamoto, Noriyuki Sugo

Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming
RV Sampaio, JR Sangalli, THC De Bem, DR Ambrizi, M del Collado, A Bridi, ACFCM Ávila, CH Macabelli, LJ Oliveira, JC da Silveira, MR Chiaratti, F Perecin, FF Bressan, LC Smith, PJ Ross, FV Meirelles

Escort-like somatic cells mediate early mouse fetal ovarian development but surface-derived Lgr5+ cells support primordial follicles
Wanbao Niu, Allan C. Spradling

Diversification of Reprogramming Trajectories Revealed by Parallel Single-cell Transcriptome and Chromatin Accessibility Sequencing
Qiaorui Xing, Chadi El Farran, Pradeep Gautam, Yu Song Chuah, Tushar Warrier, Cheng-Xu Delon Toh, Nam-Young Kang, Shigeki Sugii, Young-Tae Chang, Jian Xu, James Collins, George Daley, Hu Li, Li-Feng Zhang, Yuin-Han Loh

Emergence of neuronal diversity during vertebrate brain development
Bushra Raj, Jeffrey A. Farrell, Aaron McKenna, Jessica L. Leslie, Alexander F. Schier

A single-cell transcriptional atlas identifies extensive heterogeneity in the cellular composition of tendons
Jacob B Swanson, Andrea J De Micheli, Nathaniel P Disser, Leandro M Martinez, Nicholas R Walker, Benjamin D Cosgrove, Christopher L Mendias

Gene expression heterogeneity during brain development and aging: temporal changes and functional consequences
Ulas Isildak, Mehmet Somel, Janet Thornton, Handan Melike Donertas

Testes of DAZL null sheep lack spermatogonia and maintain normal somatic cells
Zachariah McLean, Sarah Jane Appleby, Jingwei Wei, Russell Grant Snell, Bjorn Oback

The 3D genome shapes the regulatory code of developmental genes
Julien Mozziconacci (LPTMC, MNHN), Mélody Merle (LPTMC), Annick Lesne (LPTMC, IGMM)

| Stem cells, regeneration & disease modelling

Quantitative classification of chromatin dynamics reveals regulators of intestinal stem cell differentiation
Jesse R Raab, Deepthi Y Tulasi, Kortney E Wager, Jeremy M Morowitz, Scott T Magness, Adam D Gracz

Paracrine and autocrine R-spondin signalling is essential for the maintenance and differentiation of renal stem cells
Valerie P.I. Vidal, Elodie Gregoire, Emmanuelle Szenker, Marc Leushacke, Bruno Reversade, Marie-Christine Chaboissier, Andreas Schedl

Mapping Human Pluripotent Stem Cell Derived Erythroid Differentiation by Single-Cell Transcriptome Analysis
Zijuan Xin, Wei Zhang, Shangjin Gong, Junwei Zhu, Yanming Li, Zhaojun Zhang, Xiangdong Fang

Genetically variant human pluripotent stem cells selectively eliminate wild-type counterparts through YAP-mediated cell competition
Christopher J. Price, Dylan Stavish, Paul J. Gokhale, Samantha Sargeant, Joanne Lacey, Tristan A. Rodriguez, Ivana Barbaric

Developmental trajectory of pre-hematopoietic stem cell formation from endothelium
Qin Zhu, Peng Gao, Joanna Tober, Laura Bennett, Changya Chen, Yasin Uzun, Yan Li, Melanie Mumau, Wenbao Yu, Bing He, Nancy A. Speck, Kai Tan

Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells
Peng Gao, Changya Chen, Elizabeth D. Howell, Yan Li, Joanna Tober, Yasin Uzun, Bing He, Long Gao, Qin Zhu, Arndt Siekmann, Nancy A. Speck, Kai Tan

Lfc/Arhgef2 regulates mitotic spindle orientation in hematopoietic stem and progenitor cells and is essential for productive hematopoiesis
Derek C.H. Chan, Ana Vujovic, Joshua Xu, Victor Gordon, Nicholas Wong, Laura P.M.H. de Rooij, Cailin E Joyce, Jose La Rose, Maria-Jose Sandi, Bradley W Doble, Carl D. Novina, Robert K. Rottapel, Kristin J Hope

Multi-differentiation potential is necessary for optimal tenogenesis of tendon stem cells
Ibtesam Rajpar, Jennifer G. Barrett

Evolving Transcriptomic Signature of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells With Age
Grace E. Lidgerwood, Anne Senabouth, Casey J.A. Smith-Anttila, Vikkitharan Gnanasambandapillai, Dominik C. Kaczorowski, Daniela Amann-Zalcenstein, Erica L. Fletcher, Shalin H. Naik, Alex W. Hewitt, Joseph Powell, Alice Pébay

Depletion of H3K79 methylation specifically enhances reprogramming to pluripotency but not transdifferentiation
Coral K Wille, Rupa Sridharan

Nucleosides rescue replication-mediated genome instability of human pluripotent stem cells
Jason A Halliwell, Thomas J.R. Frith, Christopher J Price, Owen Laing, Oliver J Bower, Dylan Stavish, Paul Gokhale, Zoe Hewitt, Sherif El-Khamisy, Ivana Barbaric, Peter W Andrews

Defining Essential Enhancer for Pluripotent stem cells using Features Oriented CRISPR-Cas9 Screen
Hao Fei Wang, Tushar Warrier, Chadi EL Farran, Zheng Zihao, Qiao Rui Xing, Melissa J Fullwood, Li-Feng Zhang, Hu Li, Jian Xu, Tit-Meng Lim, Yuin-Han Loh

Early Inhibition of Retinoic Acid Signaling Rapidly Generates Cardiomyocytes Expressing Ventricular Markers from Human Induced Pluripotent Stem Cells
Pranav Machiraju, Joshua Huang, Fatima Iqbal, Yiping Liu, Xuemei Wang, Chad Bousman, Steven C. Greenway

Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent states
Fabio Spada, Sarah Schiffers, Angie Kirchner, Yingqian Zhang, Olesea Kosmatchev, Eva Korytiakova, René Rahimoff, Charlotte Ebert, Thomas Carell

UFMylation of MRE11 is essential for maintenance of telomere length and hematopoietic stem cell survival
Lara Lee, Ana Belen Perez Oliva, Dmitri Churikov, Elena Martinez-Balsalobre, Joshua Peter, Dalicya Rahmouni, Gilles Audoly, Violette Azzoni, Stephane Audebert, Luc Camoin, Victoriano Mulero, Maria L. Cayuela, Vincent Geli, Yogesh Kulathu, Christophe Lachaud

G-Protein signaling accelerates stem cell divisions in Drosophila males
Manashree Malpe, Leon F. McSwain, Karl Kudyba, Chun L. Ng, Jennie Nicholson, Maximilian Brady, Yue Qian, Vinay Choksi, Alicia G. Hudson, Benjamin B. Parrott, Cordula Schulz

Energy Expenditure during Cell Spreading Induces AMPK Activation and Regulates the Mechanoresponse of Stem Cells
Jing Xie, Min Bao, Xinyu Hu, Werner J. H. Koopman, Wilhelm T. S. Huck

Direct synthesis of self-organized blastocyst-like cysts derived from human pluripotent stem cells
Xiaopeng Wen, Shiho Terada, Koki Yoshimoto, Ken-ichiro Kamei

Early life stress decreases the proliferation and numbers of adult hypothalamic neural stem cells
Pascal Bielefeld, Maralinde R. Abbink, Anna R. Davidson, Paul J. Lucassen, Aniko Korosi, Carlos P. Fitzsimons

Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Undergo Clonal Expansion In Vivo
Danny El-Nachef, Darrian Bugg, Kevin M. Beussman, Amy M. Martinson, Charles E. Murry, Nathan J. Sniadecki, Jennifer Davis

Enhanced maturation of human stem cell derived interneurons by mTOR activation
Jianhua Chu, Megan L. Fitzgerald, Neha Sehgal, William Manley, Shane Fitzgerald, Harrison Naung, Ethan M. Goldberg, Stewart A. Anderson

Brainstem organoids from human pluripotent stem cells contain neural crest population
Nobuyuki Eura, Takeshi K. Matsui, Joachim Luginbühl, Masaya Matsubayashi, Hitoki Nanaura, Tomo Shiota, Kaoru Kinugawa, Naohiko Iguchi, Takao Kiriyama, Canbin Zheng, Tsukasa Kouno, Yan Jun Lan, Pornparn Kongpracha, Pattama Wiriyasermkul, Yoshihiko M. Sakaguchi, Riko Nagata, Tomoya Komeda, Naritaka Morikawa, Fumika Kitayoshi, Miyong Jong, Shinko Kobashigawa, Mari Nakanishi, Masatoshi Hasegawa, Yasuhiko Saito, Takashi Shiromizu, Yuhei Nishimura, Takahiko Kasai, Maiko Takeda, Hiroshi Kobayashi, Yusuke Inagaki, Yasuhito Tanaka, Manabu Makinodan, Toshifumi Kishimoto, Hiroki Kuniyasu, Shushi Nagamori, Alysson R. Muotri, Jay W. Shin, Kazuma Sugie, Eiichiro Mori

A saturating mutagenesis CRISPR-Cas9 mediated functional genomic screen identifies cis- and trans- regulatory elements of Oct4 in embryonic stem cells
Matthew C. Canver, Pratibha Tripathi, Michael J. Bullen, Yogesh Kumar, Moshe Olshansky, Stephen J. Turner, Samuel Lessard, Luca Pinello, Stuart H. Orkin, Partha Pratim Das

Adult Muscle Stem Cell Self-Renewal Induced by Endurance Exercise is Mediated by Inhibition of Mitochondrial Oxygen Consumption
Phablo Abreu, Alicia J. Kowaltowski

Single-Cell Signalling Analysis of Heterocellular Organoids
Xiao Qin, Jahangir Sufi, Petra Vlckova, Pelagia Kyriakidou, Sophie E. Acton, Vivian S. W. Li, Mark Nitz, Christopher J. Tape

Biomimetic Aorta-Gonad-Mesonephros on-a-Chip to Study Human Developmental Hematopoiesis
Ryohichi Sugimura, Ryo Ohta, Chihiro Mori, Alina Li, Takafumi Mano, Emi Sano, Kaori Kosugi, Tatsutoshi Nakahata, Akira Niwa, Megumu K Saito, Yu-suke Torisawa

A novel microRNA-based strategy to expand the differentiation potency of stem cells
María Salazar-Roa, Marianna Trakala, Mónica Álvarez-Fernández, Fátima Valdés-Mora, Cuiqing Zhong, Jaime Muñoz, Yang Yu, Timothy J. Peters, Osvaldo Graña, Rosa Serrano, Elisabet Zapatero-Solana, María Abad, María José Bueno, Marta Gómez de Cedrón, José Fernández-Piqueras, Manuel Serrano, María A. Blasco, Da-Zhi Wang, Susan J. Clark, Juan Carlos Izpisua-Belmonte, Sagrario Ortega, Marcos Malumbres

Allele-specific open chromatin in human iPSC neurons elucidates functional non-coding disease variants
Siwei Zhang, Hanwen Zhang, Min Qiao, Yifan Zhou, Siming Zhao, Alena Kozlova, Jianxin Shi, Alan R. Sanders, Gao Wang, Subhajit Sengupta, Siobhan West, Michael Streit, Chad A. Cowan, Mengjie Chen, Zhiping P. Pang, Pablo V. Gejman, Xin He, Jubao Duan

Mass spectrometry analysis of mouse hematopoietic stem cells and their progenitors reveals differential expression within and between proteome and transcriptome throughout adult and aged hematopoiesis
Balyn W. Zaro, Joseph J. Noh, Victoria L. Mascetti, Janos Demeter, Benson M. George, Monika Zukowska, Gunsagar S. Gulati, Rahul Sinha, Rachel M. Morganti, Allison M. Banuelos, Allison Zhang, Peter K. Jackson, Irving L. Weissman

von Willebrand Factor D and EGF Domains is an evolutionarily conserved and required feature of blastemas capable of multi-tissue appendage regeneration
N.D. Leigh, S. Sessa, A.C. Dragalzew, D. Payzin-Dogru, J.F. Sousa, A.N. Aggouras, K. Johnson, G.S. Dunlap, B.J. Haas, M. Levin, I. Schneider, J.L. Whited

Regenerative capacity in Drosophila imaginal discs is controlled by damage-responsive, maturity-silenced enhancers
Robin E. Harris, Michael J. Stinchfield, Spencer L. Nystrom, Daniel J. McKay, Iswar K. Hariharan

UNC-16/JIP3 negatively regulates actin dynamics dependent on DLK-1 and microtubule dynamics independent of DLK-1 in regenerating neurons
Sucheta S. Kulkarni, Vidur Sabharwal, Seema Sheoran, Atrayee Basu, Kunihiro Matsumoto, Naoki Hisamoto, Anindya Ghosh-Roy, Sandhya P. Koushika

Axon-dependent expression of YAP/TAZ mediates Schwann cell remyelination but not proliferation after nerve injury
Matthew Grove, Hyunkyoung Lee, Young-Jin Son

Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury
William Renthal, Ivan Tochitsky, Lite Yang, Yung-Chih Cheng, Emmy Li, Riki Kawaguchi, Daniel H. Geschwind, Clifford J. Woolf

Regeneration of dorsal spinal cord neurons after injury via in situ NeuroD1-mediated astrocyte-to-neuron conversion
Brendan Puls, Yan Ding, Fengyu Zhang, Mengjie Pan, Zhuofan Lei, Zifei Pei, Mei Jiang, Yuting Bai, Cody Forsyth, Morgan Metzger, Tanvi Rana, Lei Zhang, Xiaoyun Ding, Matthew Keefe, Alice Cai, Austin Redilla, Michael Lai, Kevin He, Hedong Li, Gong Chen

PI 3-kinase delta enhances axonal PIP3 to support axon regeneration in the adult CNS
Amanda C Barber, Rachel S Evans, Bart Nieuwenhuis, Craig S Pearson, Joachim Fuchs, Amy R MacQueen, Susan van Erp, Barabara Haenzi, Lianne A Hulshof, Andrew Osborne, Raquel Conceicao, Sarita S Deshpande, Joshua Cave, Charles ffrench-Constant, Patrice D Smith, Klaus Okkenhaug, Britta J Eickholt, Keith R Martin, James W Fawcett, Richard Eva

Expressing of Cytochrome-c, ADAM 17, Wnt-5a, and Hedgehog gene during the tissue regeneration of digit tip mice (Mus musculus) var Swiss Webster post amputation
Titta Novianti, Febriana Dwi Wahyuni, It Jamilah, Syafruddin Ilyas

Peripheral Nerve Repair Using Tissue Engineered “Living Scaffolds” Comprised of Stretch-Grown Aligned Axonal Tracts Promotes Survival of Spinal Cord Motor Neurons
Joseph C. Maggiore, Justin C. Burrell, Kevin D. Browne, Kritika S. Katiyar, Franco A. Laimo, Zarina Ali, Hilton M. Kaplan, Joseph M. Rosen, D. Kacy Cullen

Glioblastoma stem cells induce quiescence in surrounding neural stem cells via Notch signalling
Katerina Lawlor, Maria Angeles Marques-Torrejon, Gopuraja Dharmalingham, Yasmine El-Azhar, Michael D. Schneider, Steven M. Pollard, Tristan A. Rodríguez

Loss of BICD2 in muscle drives motor neuron loss in a developmental form of spinal muscular atrophy
AM Rossor, JN Sleigh, M Groves, F Muntoni, MM Reilly, CC Hoogenraad, G Schiavo

Patient-specific functional genomics and disease modeling suggest a role for LRP2 in Hypoplastic Left Heart Syndrome
Xing Li, Almudena Martinez-Fernandez, Maria Azzurra Missinato, Jeanne L Theis, Georg Vogler, Tanja Nielsen, Stanley M Walls, Katja Birker, Jared M Evans, Megan M O’Byrne, Zachary C Fogarty, Karen Ocorr, André Terzic, Rolf Bodmer, Alexandre R Colas, Timothy J Nelson, Timothy M Olson

Expanded huntingtin CAG repeats disrupt the balance between neural progenitor expansion and differentiation in human cerebral organoids
Jinqiu Zhang, Jolene Ooi, Kagistia Hana Utami, Sarah R. Langley, Obed Akwasi Aning, Dong Shin Park, Magdalena Renner, Shiming Ma, Chit Fang Cheok, Juergen A. Knoblich, Florent Ginhoux, Enrico Petretto, Mahmoud A. Pouladi

Distinct roles for the Charcot-Marie-Tooth disease-causing endosomal regulators Mtmr5 and Mtmr13 in axon radial sorting and Schwann cell myelination
Anna E. Mammel, Katherine C. Delgado, Andrea L. Chin, Alec F. Condon, Jo Q. Hill, Sue A. Aicher, Yingming Wang, Lev M. Fedorov, Fred L. Robinson

“mir152 hypomethylation, potentially triggered by embryonic hypoxia, as a common mechanism for non-syndromic cleft lip/palate”
Lucas Alvizi, Luciano Abreu Brito, Bárbara Bischain, Camila Bassi Fernandes da Silva, Sofia Ligia Guimaraes Ramos, Gerson Shigeru Kobayashi, Jaqueline Wang, Maria Rita Passos-Bueno

Induced pluripotent stem cell-derived primary proprioceptive neurons as Friedreich ataxia cell model
Chiara Dionisi, Myriam Rai, Marine Chazalon, Serge N. Schiffmann, Massimo Pandolfo

Gene expression in patient-derived neural progenitors implicates WNT5A signaling in the etiology of schizophrenia
Oleg V Evgrafov, Chris Armoskus, Bozena B Wrobel, Valeria N Spitsyna, Tade Souaiaia, Jennifer S. Herstein, Christopher P Walker, Joseph D Nguyen, Adrian Camarena, Jonathan R Weitz, Jae Mun ‘Hugo’ Kim, Edder Lopez Duarte, Kai Wang, George M Simpson, Janet L Sobell, Helena Medeiros, Michele T Pato, Carlos N Pato, James A Knowles

| Plant development

Comparative transcriptomics of a monocotyledonous geophyte reveals shared molecular mechanisms of underground storage organ formation
Carrie M. Tribble, Jesús Martínez-Gómez, Fernando Alzate-Guarin, Carl J. Rothfels, Chelsea D. Specht

Initiation and regulation of vascular tissue identity in the Arabidopsis embryo
Margot E. Smit, Cristina I. Llavata-Peris, Mark Roosjen, Henriette van Beijnum, Daria Novikova, Victor Levitsky, Daniel Slane, Gerd Jürgens, Victoria Mironova, Siobhan M. Brady, Dolf Weijers

Identification of conserved gene regulatory networks that integrate environmental sensing and growth in the root cambium
Goh Choe, Nam Van Hoang, Yi Zheng, Ana Cecilia Aliaga Fandiño, Jaeryung Hur, Inyoung Sung, Hongryul Ahn, Sun Kim, Zhangjun Fei, Ji-Young Lee

A cell surface arabinogalactan-peptide influences root hair cell fate
Cecilia Borassi, Javier Gloazzo Dorosz, Martiniano M. Ricardi, Laercio Pol Fachin, Mariana Carignani Sardoy, Eliana Marzol, Silvina Mangano, Diana Rosa Rodríguez Garcia, Javier Martínez Pacheco, Yossmayer del Carmen Rondón Guerrero, Silvia M. Velasquez, Bianca Villavicencio, Marina Ciancia, Georg Seifert, Hugo Verli, José M. Estevez

Chloroplast nucleoids are highly dynamic in ploidy, number, and structure during angiosperm leaf development
Stephan Greiner, Hieronim Golczyk, Irina Malinova, Tommaso Pellizzer, Ralph Bock, Thomas Börner, Reinhold G. Herrmann

The Manifold Actions of Signaling Peptides on Subcellular Dynamics of a Receptor Specify Stomatal Cell Fate
Xingyun Qi, Michal Maes, Scott Zeng, Keiko U Torii

ALTERED MERISTEM PROGRAM1 regulates leaf identity independent of miR156-mediated translational repression
Jim P. Fouracre, Victoria J. Chen, R. Scott Poethig

The TARANI/ UBIQUITIN SPECIFIC PROTEASE 14 destabilizes the AUX/IAA transcriptional repressors and regulates auxin response in Arabidopsis thaliana
Parinita Majumdar, Premananda Karidas, Imran Siddiqi, Utpal Nath

DEK influences the trade-off between growth and arrest via H2A.Z-nucleosomes in Arabidopsis
Anna Brestovitsky, Daphne Ezer, Sascha Waidmann, Sarah L. Maslen, Martin Balcerowicz, Sandra Cortijo, Varodom Charoensawan, Claudia Martinho, Daniela Rhodes, Claudia Jonak, Philip A Wigge

mRNA decapping machinery targets transcripts of the LBD3/ASL9 transcription factor to authorize formation of apical hook and lateral roots in Arabidopsis
Zhangli Zuo, Milena Edna Roux, Eleazar Rodriguez, Jonathan Renaud Chevalier, Yasin F. Dagdas, Takafumi Yamashino, Morten Petersen

Lateral mechanical impedance rather than frontal, promotes cortical expansion of roots
Xuanjun Feng, Jing Xiong, Yue Hu, Liteng Pan, Zhengqiao Liao, Xuemei Zhang, Wei Guo, Fengkai Wu, Jie Xu, Erliang Hu, Hai Lan, Yanli Lu

Altering PRC2 activity partially suppresses ddm1 mutant phenotypes in Arabidopsis
Martin Rougée, Leandro Quadrana, Jérôme Zervudacki, Vincent Colot, Lionel Navarro, Angélique Deleris

Specificity in auxin responses is not explained by the promoter preferences of activator ARFs
Amy Lanctot, Mallorie Taylor-Teeples, Erika A. Oki, Jennifer L. Nemhauser

EXO70A2 is critical for the exocyst complex function in Arabidopsis pollen
Vedrana Marković, Fatima Cvrčková, Martin Potocký, Přemysl Pejchar, Eva Kollárová, Ivan Kulich, Lukáš Synek, Viktor Žárský

Golgi anti-apoptotic proteins are evolutionarily conserved ion channels that regulate cell death in plants
Maija Sierla, David L Prole, Nuno Saraiva, Guia Carrara, Natalia Dinischiotu, Aleksia Vaattovaara, Michael Wrzaczek, Colin W Taylor, Geoffrey L Smith, Bart Feys

Genome-wide association study for maize leaf cuticular conductance identifies candidate genes involved in the regulation of cuticle development
Meng Lin, Susanne Matschi, Miguel Vasquez, James Chamness, Nicholas Kaczmar, Matheus Baseggio, Michael Miller, Ethan L. Stewart, Pengfei Qiao, Michael J. Scanlon, Isabel Molina, Laurie G. Smith, Michael A. Gore

Separable regulation of POW1 in TAF2-mediated grain development and BR-mediated leaf angle formation in rice
Li Zhang, Ruci Wang, Yueming Wang, Yufang Xu, Shuang Fang, Jinfang Chu, Shanguo Yao

Regulation of electron transport is essential for photosystem I stability and plant growth
Mattia Storti, Anna Segalla, Marco Mellon, Alessandro Alboresi, Tomas Morosinotto

A simple method for spray-on gene editing in planta
Cara Doyle, Katie Higginbottom, Thomas A. Swift, Mark Winfield, Christopher Bellas, David Benito-Alifonso, Taryn Fletcher, M. Carmen Galan, Keith Edwards, Heather M. Whitney

qKW9 encodes a pentatricopeptide repeat protein affecting photosynthesis and grain filling in maize
Juan Huang, Gang Lu, Lei Liu, Mohammad Sharif Raihan, Jieting Xu, Liumei Jian, Lingxiao Zhao, Thu M. Tran, Qinghua Zhang, Jie Liu, Wenqiang Li, Cunxu Wei, David M. Braun, Qing Li, Alisdair R. Fernie, David Jackson, Jianbing Yan

Transcriptomic analysis of developing seeds in a wheat mutant RSD32 with reduced seed dormancy
Kazuhide Rikiishi, Manabu Sugimoto, Masahiko Maekawa

Evo-devo & evo

Adult chondrogenesis and spontaneous cartilage repair in the skate, Leucoraja erinacea
Aleksandra Marconi, Amy Hancock-Ronemus, J. Andrew Gillis

Dual Roles of the Retinal Pigment Epithelium and Lens in Cavefish Eye Degeneration
Li Ma, Mandy Ng, Corine M. van der Weele, Masato Yoshizawa, William R. Jeffery

Evolution of the gastrointestinal tract morphology and plasticity in cave-adapted Mexican tetra, Astyanax mexicanus
Misty R. Riddle, Fleur Damen, Ariel Aspiras, Julius A. Tabin, Suzanne McGaugh, Clifford J. Tabin

Developmental transcriptomic analysis of the cave-dwelling crustacean, Asellus aquaticus
Joshua B. Gross, Dennis A. Sun, Brian M. Carlson, Sivan Brodo-Abo, Meredith E. Protas

Comparative transcriptomics reveal distinct patterns of gene expression conservation through vertebrate embryogenesis
Megan E. Chan, Pranav S. Bhamidipati, Heather J. Goldsby, Arend Hintze, Hans A. Hofmann, Rebecca L. Young

The hematopoietic landscape at single-cell resolution reveals unexpected stem cell features in naked mole-rats
Stephan Emmrich, Marco Mariotti, Masaki Takasugi, Maggie E. Straight, Alexandre Trapp, Vadim N. Gladyshev, Andrei Seluanov, Vera Gorbunova

Different roles of eye absent in the basal ovarian follicle and germarium of developing cockroach ovaries
S. Ramos, F. Chelemen, V. Pagone, N. Elshaer, P. Irles, M.D. Piulachs

Multiple roles for laccase2 in butterfly wing pigmentation, scale development, and cuticle tanning
Ceili L. Peng, Anyi Mazo-Vargas, Benjamin J. Brack, Robert D. Reed

An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica
Marion Lechable, Alexandre Jan, Brandon Weissbourd, Julie Uveira, Loann Gissat, Sophie Collet, Laurent Gilletta, Sandra Chevalier, Lucas Leclère, Sophie Peron, Carine Barreau, Régis Lasbleiz, Evelyn Houliston, Tsuyoshi Momose

Environmental specificity in Drosophila-bacteria symbiosis affects host developmental plasticity
Robin Guilhot, Antoine Rombaut, Anne Xuéreb, Kate Howell, Simon Fellous

Synergistic cues from diverse bacteria enhance multicellular development in a choanoflagellate
Ella V. Ireland, Arielle Woznica, Nicole King

Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
Marnie L Freckelton, Brian T. Nedved, You-Sheng Cai, Shugeng Cao, Helen Turano, Rosanna A. Alegado, Michael G. Hadfield

Topological constraints in early multicellularity favor reproductive division of labor
David Yanni, Shane Jacobeen, Pedro Márquez-Zacarías, Joshua S Weitz, William C. Ratcliff, Peter J. Yunker

Bacterial contribution to genesis of the novel germ line determinant oskar
Leo Blondel, Tamsin E. M. Jones, Cassandra G. Extavour

Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)
Paulino Martínez, Diego Robledo, Xoana Taboada, Andrés Blanco, Antonio Gómez-Tato, Blanca Álvarez-Blázquez, Santiago Cabaleiro, Francesc Piferrer, Carmen Bouza, Ana M. Viñas

A screen for gene paralogies delineating evolutionary branching order of early Metazoa
Albert J Erives, Bernd Fritzsch

Primate-restricted KRAB zinc finger proteins and target retrotransposons control gene expression in human neurons
Priscilla Turelli, Christopher Playfoot, Dephine Grun, Charlène Raclot, Julien Pontis, Alexandre Coudray, Christian Thorball, Julien Duc, Eugenia Pankevich, Bart Deplancke, Volker Busskamp, Didier Trono

Cell biology

Spatial organization of cortical actin alignments for the ooplasmic segregation of ascidian Ciona eggs
Hirokazu Ishii, Tomomi Tani

Cell cycle S-phase arrest drives cell extrusion
Vivek K. Dwivedi, Carlos Pardo-Pastor, Rita Droste, Daniel P. Denning, Jody Rosenblatt, H. Robert Horvitz

Transcriptional reprogramming in fused cells is triggered by plasma-membrane diminution
Daniel Feliciano, Isabel Espinosa-Medina, Aubrey Weigel, Kristin M. Milano, Zhonghua Tang, Tzumin Lee, Harvey J. Kliman, Seth M. Guller, Carolyn M. Ott, Jennifer Lippincott-Schwartz

The Somatic Golgi nucleates microtubules that are directed by Kinesin-2 to maintain microtubule polarity within neurons
Amrita Mukherjee, Paul Brooks, Fred Bernard, Antoine Guichet, Paul T. Conduit

DISTINCT ACTIN-DEPENDENT NANOSCALE ASSEMBLIES UNDERLIE THE DYNAMIC AND HIERARCHICAL ORGANIZATION OF E-CADHERIN.
Rumamol Chandran, Girish Kale, Jean-Marc Philippe, Thomas Lecuit, Satyajit Mayor

The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts
Nisha Venugopal, Ananga Ghosh, Hardik Gala, Ajoy Aloysius, Neha Vyas, Jyotsna Dhawan

Non-stem progenitors enable coordinated changes in gut epithelial cell-type composition
Laura E. Sanman, Ina W. Chen, Jake M. Bieber, Veronica Steri, Byron Hann, Lani F. Wu, Steven J. Altschuler

Cortical cell stiffness is independent of substrate mechanics
Johannes Rheinlaender, Andrea Dimitracopoulos, Bernhard Wallmeyer, Nils M. Kronenberg, Kevin J. Chalut, Malte C. Gather, Timo Betz, Guillaume Charras, Kristian Franze

N-cadherin SPRY motifs bind unconventionally-secreted Fbxo45 and regulate multipolar neuron migration
Youn Na, Elif Kon, Hong Cao, Yves Jossin, Jonathan A. Cooper

Mitochondria tether to Focal Adhesions during cell migration and regulate their size
Redaet Daniel, Abebech Mengeta, Patricia Bilodeau, Jonathan M Lee

Serine and glycine are essential for human muscle progenitor cell population expansion
Brandon J. Gheller, Jamie E. Blum, Erica L. Bender, Mary E. Gheller, Esther W. Lim, Michal K. Handzlik, Patrick J. Stover, Martha S. Field, Benjamin D. Cosgrove, Christian M. Metallo, Anna E. Thalacker-Mercer

Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings
Stéphane Vassilopoulos, Solène Gibaud, Angélique Jimenez, Ghislaine Caillol, Christophe Leterrier

Lgr5+ telocytes are a signaling hub at the intestinal villus tip
Keren Bahar Halpern, Hassan Massalha, Rachel K. Zwick, Andreas E. Moor, David Castillo-Azofeifa, Milena Rozenberg, Lydia Farack, Adi Egozi, Dan R. Miller, Inna Averbukh, Yotam Harnik, Noa Weinberg-Corem, Frederic J. de Sauvage, Ido Amit, Ophir D. Klein, Michal Shoshkes-Carmel, Shalev Itzkovitz

Novel function of TRIP6, in brain ciliogenesis
Shalmali Shukla, Pavel Urbanek, Lucien Frappart, Ronny Hänold, Sigrun Nagel, Shamci Monajembashi, Paulius Grigaravicius, Woo Kee Min, Alicia Tapias, Olivier Kassel, Heike Heuer, Zhao-Qi Wang, Aspasia Ploubidou, Peter Herrlich

Nanotopography enhances dynamic remodeling of tight junction proteins through cytosolic complexes
Xiao Huang, Xiaoyu Shi, Mollie Eva Hansen, Cameron L. Nemeth, Anna Celli, Bo Huang, Theodora Mauro, Michael Koval, Tejal Desai

Modelling

Predicting Evolutionary Transitions in Tooth Complexity With a Morphogenetic Model
Aidan M. C. Couzens, Karen E. Sears, Martin Rücklin

Notch signaling and taxis mechanims regulate early stage angiogenesis: A mathematical and computational model
Rocío Vega, Manuel Carretero, Rui D.M. Travasso, Luis L. Bonilla

Cell-based simulations of Notch-dependent cell differentiation on growing domains
Anna Stopka, Marcelo Boareto, Dagmar Iber

Hierarchical modeling of mechano-chemical dynamics of epithelial sheets across cells and tissue
Yoshifumi Asakura, Yohei Kondo, Kazuhiro Aoki, Honda Naoki

Modeling stripe formation on growing zebrafish tailfins
Alexandria Volkening, Madeline R Abbott, Dorothy Catey, Neil Chandra, Bethany Dubois, Francesca Lim, Bjorn Sandstede

Regeneration comes for free with biological development in a generative Boolean model
Somya Mani, Tsvi Tlusty

Self-sustained Planar Intercalations due to Mechanosignaling Feedbacks Lead to Robust Axis Extension during Morphogenesis
Samira Anbari, Javier Buceta

Cellular crowding guides and debundles the microtubule cytoskeleton
A. Z. Płochocka, N. A. Bulgakova, L. Chumakova

Mathematical modeling of plant cell fate transitions controlled by hormonal signals
Filip Z. Klawe, Thomas Stiehl, Peter Bastian, Christophe Gaillochet, Jan U. Lohmann, Anna Marciniak-Czochra

An integrated multiscale, multicellular skin model
Ryan Tasseff, Boris Aguilar, Simon Kahan, Seunghwa Kang, Charles C. Bascom, Robert J. Isfort

Tuning cell motility via cell tension with a mechanochemical cell migration model
K. Tao, J. Wang, X. Kuang, W. Wang, F. Liu, L. Zhang

Mathematical models for cell migration: a nonlocal perspective
Li Chen, Kevin Painter, Christina Surulescu, Anna Zhigun

Tools & resources

Large-scale transgenic Drosophila resource collections for loss- and gain-of-function studies
Jonathan Zirin, Yanhui Hu, Luping Liu, Donghui Yang-Zhou, Ryan Colbeth, Dong Yan, Ben Ewen-Campen, Rong Tao, Eric Vogt, Sara VanNest, Cooper Cavers, Christians Villalta, Aram Comjean, Jin Sun, Xia Wang, Yu Jia, Ruibao Zhu, Pin Peng, Jinchao Yu, Da Shen, Yuhao Qiu, Limmond Ayisi, Henna Ragoowansi, Ethan Fenton, Senait Efrem, Annette Parks, Kuniaki Saito, Shu Kondo, Liz Perkins, Stephanie E. Mohr, Jianquan Ni, Norbert Perrimon

A resource of targeted mutant mouse lines for 5,061 genes
Marie-Christine Birling, Atsushi Yoshiki, David J Adams, Shinya Ayabe, Arthur L Beaudet, Joanna Bottomley, Allan Bradley, Steve DM Brown, Antje Bürger, Wendy Bushell, Francesco Chiani, Hsian-Jean Genie Chin, Skevoulla Christou, Gemma F Codner, Francesco J DeMayo, Mary E Dickinson, Brendan Doe, Leah Rae Donahue, Martin D Fray, Alessia Gambadoro, Xiang Gao, Marina Gertsenstein, Alba Gomez-Segura, Leslie O Goodwin, Jason D Heaney, Yann Hérault, Martin Hrabe de Angelis, Si-Tse Jiang, Monica J Justice, Petr Kasparek, Ruairidh E King, Ralf Kühn, Ho Lee, Young Jae Lee, Zhiwei Liu, K C Kent Lloyd, Isabel Lorenzo, Ann-Marie Mallon, Colin McKerlie, Terrence F Meehan, Stuart Newman, Lauryl MJ Nutter, Goo Taeg Oh, Guillaume Pavlovic, Ramiro Ramirez-Solis, Barry Rosen, Edward J Ryder, Luis A Santos, Joel Schick, John R Seavitt, Radislav Sedlacek, Claudia Seisenberger, Je Kyung Seong, William C Skarnes, Tania Sorg, Karen P Steel, Masaru Tamura, Glauco P Tocchini-Valentini, Chi-Kuang Leo Wang, Hannah Wardle-Jones, Marie Wattenhofer-Donzé, Sara Wells, Brandon J Willis, Joshua A Wood, Wolfgang Wurst, Ying Xu, IMPC Consortium, Lydia Teboul, Stephen A Murray

Minimal genome-wide human CRISPR-Cas9 library
Emanuel Gonçalves, Mark Thomas, Fiona M Behan, Gabriele Picco, Clare Pacini, Felicity Allen, David Parry-Smith, Francesco Iorio, Leopold Parts, Kosuke Yusa, Mathew J Garnett

A portable and cost-effective microfluidic system for massively parallel single-cell transcriptome profiling
Chuanyu Liu, Tao Wu, Fei Fan, Ya Liu, Liang Wu, Michael Junkin, Zhifeng Wang, Yeya Yu, Weimao Wang, Wenbo Wei, Yue Yuan, Mingyue Wang, Mengnan Cheng, Xiaoyu Wei, Jiangshan Xu, Quan Shi, Shiping Liu, Ao Chen, Ou Wang, Ming Ni, Wenwei Zhang, Zhouchun Shang, Yiwei Lai, Pengcheng Guo, Carl Ward, Giacomo Volpe, Lei Wang, Huan Zheng, Yang Liu, Brock A. Peters, Jody Beecher, Yongwei Zhang, Miguel A. Esteban, Yong Hou, Xun Xu, I-Jane Chen, Longqi Liu

Primary mammary organoid model of lactation and involution
Jakub Sumbal, Aurelie Chiche, Elsa Charifou, Zuzana Koledova, Han LI

Direct synthesis of self-organized blastocyst-like cysts derived from human pluripotent stem cells
Xiaopeng Wen, Shiho Terada, Koki Yoshimoto, Ken-ichiro Kamei

Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light-Activated Guide RNA
Wenyuan Zhou, Wes Brown, Anirban Bardhan, Michael Delaney, Amber S. Ilk, Randy R. Rauen, Shoeb I. Kahn, Michael Tsang, Alexander Deiters

Minimized double guide RNA libraries enable scale-limited CRISPR/Cas9 screens
Elin Madli Peets, Luca Crepaldi, Yan Zhou, Felicity Allen, Rasa Elmentaite, Guillaume Noell, Gemma Turner, Vivek Iyer, Leopold Parts

ΔSCOPE: A new method to quantify 3D biological structures and identify differences in zebrafish forebrain development
Morgan S Schwartz, Jake Schnabl, Mackenzie P.H. Litz, Benjamin S Baumer, Michael Barresi

An optimized protocol for iDISCO+ rat brain clearing, imaging, and analysis
Audrey Branch, Daniel Tward, Joshua T Vogelstein, Zhuhao Wu, Michela Gallagher

A versatile tiling light sheet microscope for cleared tissue imaging
Xiaoliang Li, Dongdong Zhang, Chunhui Wang, Xuzhao Li, Mengjie Lai, Yao Weng, Ruili Feng, Xinyi Shirley Zhang, Yanlu Chen, Jing Yu, Dongyue Wang, Rui Nie, Xiao Yang, Yongyi Chen, Bi-Chang Chen, Yi Feng, Bo Zhou, Shang Cai, Jie-Min Jia, Liang Gao

High-Resolution 3D Fluorescent Imaging of Intact Tissues
Danny El-Nachef, Amy M Martinson, Xiulan Yang, Charles E Murry, W Robb MacLellan

MACS: Rapid aqueous clearing system for three-dimensional mapping of intact organs
Jingtan Zhu, Tingting Yu, Yusha Li, Jianyi Xu, Yisong Qi, Yingtao Yao, Yilin Ma, Peng Wan, Zhilong Chen, Xiangning Li, Hui Gong, Qingming Luo, Dan Zhu

The effect of proximity on the function and energy transfer capability of fluorescent protein pairs
Jacob R. Pope, Rachel L. Johnson, W. David Jamieson, Harley L Worthy, Senthilkumar D. Kailasam, Husam Sabah Auhim, Daniel W. Watkins, Pierre Rizkallah, Oliver Castell, D. Dafydd Jones

Direct readout of neural stem cell transgenesis with an integration-coupled gene expression switch
Takuma Kumamoto, Franck Maurinot, Raphaëlle Barry, Célia Vaslin, Sandrine Vandormael-Pournin, Mickaël Le, Marion Lerat, Michel Cohen-Tannoudji, Alexandra Rebsam, Karine Loulier, Stéphane Nédelec, Samuel Tozer, Jean Livet

An extensively optimized chromatin immunoprecipitation protocol for quantitatively comparable and robust results
Wim J. de Jonge, Mariël Brok, Patrick Kemmeren, Frank C.P. Holstege

singleCellHaystack: A clustering-independent method for finding differentially expressed genes in single-cell transcriptome data
Alexis Vandenbon, Diego Diez

Cell Tracking Profiler: a user-driven analysis framework for evaluating 4D live cell imaging data
Claire Mitchell, Lauryanne Caroff, Alessandra Vigilante, Jose Alonso Solis-Lemus, Constantino Carlos Reyes-Aldasoro, Fabrice de Chaumont, Alexandre Dufour, Stephane Dallongeville, Jean-Christophe Olivo-Marin, Robert D Knight

Deep generative model embedding of single-cell RNA-Seq profiles on hyperspheres and hyperbolic spaces
Jiarui Ding, Aviv Regev

Research practice & education

bioRxiv: the preprint server for biology
Richard Sever, Ted Roeder, Samantha Hindle, Linda Sussman, Kevin-John Black, Janet Argentine, Wayne Manos, John R. Inglis

The Academic Career Readiness Assessment: Clarifying training expectations for future life sciences faculty
Laurence Clement, Jennie B. Dorman, Richard McGee

Plagiarism in Brazil: A perspective of 25,000 PhD holders across the sciences
Sonia MR Vasconcelos, Hatisaburo Masuda, Martha Sorenson, Francisco Prosdocimi, Marisa Palácios, Edson Watanabe, José Carlos Pinto, José Roberto Lapa e Silva, Adalberto Vieyra, André Pinto, Jesús Mena-Chalco, Mauricio Sant’Ana, Miguel Roig

If your P value looks too good to be true, it probably is: Communicating reproducibility and variability in cell biology
Samuel J. Lord, Katrina B. Velle, R. Dyche Mullins, Lillian K. Fritz-Laylin

Why not…

Naturally occurring fluorescence protects the eutardigrade Paramacrobiotus sp. from ultraviolet radiation
Harikumar R Suma, Swathi Prakash, Debasish Giri, Govindasamy Mugesh, Sandeep M. Eswarappa

(6 votes)

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