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Woods Hole Images round 3- the winner

Posted by , on 27 June 2013

Another set of beautiful images, and another great winner! The winner of this year’s 3rd round of the Woods Hole Embryology Course is the pig embryo!

2013Round3_B_largeThis great picture was taken by Marina Venero Galanternik (University of Utah), Rodrigo G. Arzate-Mejía (Universidad Nacional Autonoma de Mexico), Jennifer McKey (Universite Montpellier) and William Munoz (The University of Texas MD Anderson Cancer Center). It shows a colour inverted image of a skeleton preparation of a pig (Sus scrofa domesticus) embryo.

The runners-up to this competition were the Drosophila embryos by Davon Callander (Oregon State University); a fluorescently stained annelid by Eduardo Zattara (University of Maryland, College Park); and a grafted Xenopus by Elsie Place (MRC National Institute of Medical Research).

This beautiful pig embryo will feature in the cover of Development in a coming issue. As for the cover competition, there is still one round of beautiful pictures to come this year!

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There and back again…

Posted by , on 26 June 2013

We’ve all heard the aphorism that once you leave academia, you can’t go back. A little over two years ago, I wrote about my transition from academic research to scientific editing. Now, after completing my first year as an assistant professor of Biology at Reed College, I’m offering proof that lane changes, U-turns, and detours can lead you back to the ivory tower. Many people suggested that I wouldn’t be able to come back to academia when I chose to accept an editorial job at Cell, and I had every intention of sticking with my chosen path of scientific editing. After almost a year of sitting at a desk in an office that didn’t smell like acetic acid or freshly poured LB agar, a niggling feeling kept telling me that something wasn’t quite right. A few soul-searching diary entries, a couple of personality type indicators, and many conversations with friends and mentors helped me discern exactly what that feeling was trying to tell me. Scientific careers can follow many different trajectories and sometimes the “best fit” path is not always the easiest or most logical choice.

It is my hope that sharing my journey thus far helps scientists at all stages of their careers recognize that many valid career paths exist and that lane changes, U-turns, and detours are always possible. And I hope that this piece will spark conversation, here and elsewhere, about the varied and multiple careers scientists pursue.

The exit ramp
When I made the decision to leave my post-doc for the position at Cell, I focused on what the job offered: the ability to do more of what I liked – thinking and communicating science – and less of what I didn’t like – worrying about funding and dealing with the pressure to publish in high-impact journals. (The irony of working at a high-impact journal was not lost on me.) Plus the editorial job put me in the same time zone as the rest of my family. It was a logical and rational decision.

The novelty of scientific editing excited me in a way that my day-to-day work as a post-doc didn’t and, as a result, I disregarded the things that I actually enjoyed about working in academia, like the experimental troubleshooting, the microscopy, the competition to figure something out before someone else, the pipetting, the interactions with students. Working as an editor helped me hone my critical thinking skills, gave me a strong foundation in written communication, and also helped me clarify who I was, what I wanted, and what I valued. I need to make (or at least feel like I’m making) unique and creative contributions to science.

Lost without a map
In an era of tight funding and high-stakes research, my decision to leave a very good and relatively secure job for one fraught with stress and insecurity might seem a little odd, and certainly not very practical. It is, in fact, one of the least rational decisions I’ve made. And although we scientists are often known for our logic and careful reasoning, many of us are intuitive thinkers, making decisions based, at least partly, on gut instinct. This is not to say that I did not enjoy my time as an editor, because I did. I count myself incredibly fortunate to have worked as a scientific editor at Cell, and had my academic job search been unsuccessful, I’d still be handling manuscripts, recruiting papers, talking with authors, attending meetings as an editor, and savoring the opportunity to read papers and work with really smart people.

The U-turn…
Applying for assistant professorships while also working as a full-time editor forced me to focus on exactly what I wanted. While many of my post-doc friends and colleagues indiscriminately applied to 30 or 40 positions, I applied to seven. I was mindful of location (after living in a variety of places up and down the East Coast and in London, my husband and I wanted a place that had plenty of green space and the option to cycle and walk most places). I knew from multiple experiences that I wanted to work directly with undergraduate students and maintain a small, active research program. My weekends were dedicated to outlining my tentative research program and customizing my application materials for each position on my list. My scientific “U-turn” was possible, in large part, because of an incredibly generous post-doc mentor (Steve Wilson at UCL) who allowed me to resume some of the projects I’d left behind and use them as a jumping off point for my long-term research program.

The new path…
I’m not quite sure what the various search committees saw in my application, but I was extremely fortunate to have two fantastic offers to teach and do research at top-notch liberal arts schools. I love my current job at Reed College. My experiences as a high school science teacher (before grad school) and a scientific editor (before this position) endowed me with a unique and useful skillset. As a professor at small liberal arts college, I relish designing both curriculum and experiments. I enjoy reading, writing, and inviting notable scientist for seminars. I impact the future of science by teaching and mentoring undergraduates in my lab. I’ve embraced the academic lifestyle. And although our recently adopted dog, Mickey, would like to think his need for a run is what gets me up bright and early ~5am, it’s really the opportunity to share my love of research, encourage students to explore and experiment, and mentor budding scientists that get me out of bed every morning. The life of an assistant biology professor at a place like Reed can be incredibly rewarding, but it also comes with a healthy dose of stress. Fear of delivering a subpar lecture, apprehension about lab activities not working, and anxiety about my nascent research program certainly gave me some grey hairs. That said, I wouldn’t have traded this past year for anything (well, maybe more sleep). I’ve never learned as much, slept as little, worried as much, or felt as happy as I have this past year. And I wouldn’t have survived this year without the stress relief and support that my dog, husband, wonderful colleagues, mentors, and friends provided.

Even if Reed isn’t my final academic home, my journey so far has taught me to seize the opportunities that come my way, to learn and live as fully as I possibly can. And I hope that this one example of leaving and returning to academia gives you the courage to search for the career path that is right for you.

What are your experiences? Have you found your “dream job”? What have you learned from taking one of the less mainstream career paths? Please post here to continue this exploration of career trajectories.

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Postdoctoral Position – Institut Pasteur, Paris, France

Posted by , on 26 June 2013

Closing Date: 15 March 2021

We invite applications for a postdoctoral research fellow to join the lab of “Imaging and Regulation of Morphogenesis in Higher Vertebrates” at the Pasteur Institute in Paris, France. Our lab is interested in understanding the  morphogenesis of developing structures, at a cellular level. Using avian models we combine state-of-the-art live imaging methodologies, quantitative analyses, biophysical, cellular and molecular biology approaches to access the cellular dynamics of development.

This specific project aims at elucidating the cellular events underlying the initiation of limb bud formation and how such cell events are dynamically regulated at the molecular level. For more information about projects and the lab please visit: www.jgroslab.com .
The position is a two-year postdoctoral position funded by the FRM (Fondation pour la Recherche Medicale) available immediately, although the starting date is flexible. We are seeking highly motivated candidates with expertise in developmental and/or cellular biology. Experience in imaging will be positively considered.

The Pasteur Institute, located in the vibrant city of Paris, has a longstanding history of excellence in developmental biology and in science in general, with access to excellent core facilities.

Applicants should send a cover letter (describing briefly research interests), a C.V and contact information for up to 3 academic references to jgros@pasteur.fr.

Postdoc_Ad_Institut_Pasteur

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Exploring Embryology at the Woods Hole MBL

Posted by , on 25 June 2013

After two weeks of intense work in the Woods Hole MBL Embryology course, I am beginning to understand why this course compels TAs, faculty and students from around the world to return year after year. The 24 students in the 2013 Embryology class work on a diverse set of organisms including worms, mice, chick, coral, dinosaurs, and lophotrochozoans, but we are tied together by our interest in development.

Our desire to understand development brought us to this six-week course featuring daily lectures, discussion and many hours of work in the lab with a variety of model systems. It is intense work, but at the same time, it’s all play. In the last two weeks together, we’ve studied how sea urchin, nematode, and arthropod eggs turn into swimming or crawling larvae. Adding sea urchin eggs and sperm together, we watched the vitelline envelope balloon indicating fertilization. Timelapse movies captured cell divisions, gastrulation, filopodial dynamics, and organ development. With confocal microscopes, we examined localization of proteins and RNAs to understand patterning and created 3D reconstructions of embryos.

The most striking thing to me about the MBL has been not the plethora of embryos or the microscopes, but the community of scientists it builds. The course directors and many of the speakers were students of the course, with stories and fond recollections. The faculty, course directors, and TAs worked with us all day and most of the night, troubleshooting microscopes or antibody staining and providing a wealth of experience and knowledge. As a student, I have the privilege of being a part of this community, working where many scientists, including T. H. Morgan, did their summer research. Much of the rich heritage of the MBL is showcased in the WHOI library rare books room, which features Morgan’s traveling microscope, multiple Nobel prizes, and scientific texts dating back to the 1500’s.

Our time at Woods Hole so far has already changed how we look at the world. You approach murky seawater with fascination, because you will find fish and snail embryos. I look at my research with a broader perspective and am excited to discover unexpected connections between my work and my classmates’ research.  You are confident enough to tackle new techniques and ask questions boldly; to gain experience through work and interactions with faculty. I can’t wait to see what the next four weeks hold.

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Mouse Molecular Genetics 2013

Posted by , on 24 June 2013

Mouse Molecular Genetics
18-21 September 2013
Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Abstract and Bursary deadline: 19 July | Registration deadline: 7 August

We are pleased to announce that the 26th annual Mouse Molecular Genetics meeting will be held at the Wellcome Trust Genome Campus, from 18 to 21 September 2013.
This meeting is a leading forum for researchers who apply genetics and genomics techniques to address fundamental issues in mammalian biology, including stem cell biology, development, epigenetics and models of human disease.

The meeting invites leaders in these areas to present unpublished research findings, encourages junior investigators to participate in oral and poster presentations and provides a stimulating environment for the exchange of ideas. The programme will showcase the latest technical developments in genetics and engineering of the mouse genome and this year will also feature a new session devoted to cancer. We welcome abstracts from areas relevant to mammalian molecular genetics, several oral presentations be selected from submitted abstracts.

Scientific programme committee
Allan Bradley, Wellcome Trust Sanger Institute, UK
Kat Hadjantonakis, Sloan-Kettering Institute, USA
Yumiko Saga, National Institute of Genetics, Japan
Philippe Soriano, Mount Sinai School of Medicine, USA

Keynote speaker
Margaret Buckingham, The Institut Pasteur, France

Rosa Beddington Lecture
Robin Lovell-Badge, Medical Research Council, UK

Invited speakers Wendy Bickmore, University of Edinburgh, UK Thijn Brummelkamp, Netherlands Cancer Institute, The Netherlands
Elaine Dzierzak, Erasmus Stem Cell Institute, The Netherlands Anne Grapin-Botton, University of Copenhagen, Denmark Yann Herault, Centre de Recherche en Biologie and Medecine, France
Jos Jonkers, Netherlands Cancer Institute, The Netherlands Ryoichiro Kageyama, Kyoto University, Japan Haruhiko Koseki, Riken, Japan
Jinsong Li, Institute of Biochemistry and Cell Biology, China Heiko Lickert, Institute of Stem Cell Research, Germany Roland Rad, Technische Universität München, Germany Nadia Rosenthal, Imperial College London, UK Shankar Srinivas, University of Oxford, UK Azim Surani, University of Cambridge, UK Feng Zang, Broad Institute of MIT, USA

Topics will include: Organogenesis Technology Stem cells and germ cells Patterning Genetics and genomics Human disease Epigenetics Cancer

For further information and to register: https://registration.hinxton.wellcome.ac.uk/display_info.asp?id=372

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Day 5 at the ISDB- Goodbye Cancun, hello Singapore!

Posted by , on 22 June 2013

The last day of the ISDB saw also the last plenary session. One of the highlights of this session was also a talk by Patricia Delgado. Patricia works on the molecular and evolutionary basis of butterfly wing patterning. Her talk included beautiful pictures of butterflies, including an image (not from her lab) of the alphabet written on naturally occurring butterfly wing patterns.

There were two more concurrent sessions, before a few free hours in the afternoon. This being the last day in Cancun, and such a sunny day as well, conference attendees could probably be spotted in every pool/beach in Cancun! Everyone was then back to the conference center for the conference dinner. But a surprise expected us: by the entrance were three actors dressed in quite impressive mayan-inspired outfits! It became the thing to do, to take photos with them. Not even Nobel prize winners were immune, as Martin Chalfie was spotted posing!

mayan indians 2Conference attendees posing for photographs with mayans

 

The conference dinner then took place, and included the poster prize award ceremony. With three different organisations giving prizes, a lot of the students and postdocs saw their work recognized at this meeting. As is the tradition here on the Node, the winner of the poster prize at the BSDB, Aditya Saxena (who we interviewed a few weeks ago) interviewed the winner of the SDB poster prize, Kara Nordin- so look out for this interview here on the Node soon! The band then took over, and the combination of alcohol and music had the effect that it always has- a lot of scientists on the dance floor!

Although it is sad to see the end of this meeting, and leave the sunny Cancun, there is definitely something to look forward to: it was announced today that the next ISDB meeting in four years time will take place in Singapore, where the new ISDB president, Phil Ingham, is based. It seems that developmental biologists know how to choose good locations to hold their meetings!

 

Singapore bid

The successful Singapore bid to host the nest ISDB meeting

 

The Node was at the ISDB in Cancun, and we posted daily reports. In addition to this post you can read the report of day 1day 2day 3 and day 4.

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Day 4 at the ISDB- many prizes

Posted by , on 20 June 2013

Today was the day when the excellent work of many developmental biologists was recognized by the community. The first prize to be awarded was the brand new Latin American Society of Developmental Biology prize. The winner, Roberto Mayor, was awarded this prize not only for his excellent body of work on neural crest cells, but also his efforts in promoting developmental biology in Latin America- he was the founder and first president of the LASDB, and is still very much involved, running for example, an embryology course in Chile every year. The actual prize was a beautiful and unique art piece by a Brazilian artist- a very special prize indeed. On a lighter note, Roberto Mayor also received an additional prize. Marianne Bronner awarded him a more comical gift, from all the neural crest scientists who are not fortunate enough to work, like him, in the more tractable Xenopus. Roberto then delighted the audience with a great talk on the mechanisms at work in neural crest migration.

 

Roberto Mayor's prizes croppedThe two prizes received by Roberto Mayor. Any guesses on which one was the official prize?

Marianne Bronner was herself the receiver of an award today- the Conklin Medal awarded by the SDB to recognize her work. She very sweetly thanked her current and previous lab members (her ‘progeny’ as she calls them) for their hard work that made it all possible, and for having contributed for her nomination to the award. We interviewed Marianne today, so look out for the interview on the Node soon. Other awardees included Bill Wood, who was awarded the Victor Hamburger Outsanding Educator Prize, for his many contributions to the way science is taught (or should be taught) in the lecture room. Another awardee was John Fallon, who received a SDB Lifetime Achievement Award for his body of work on limb development.

Marianne Bronner talk 2Marianne Bronner gives her Conklin Medal Prize lecture

 

But the conference was not only awards. There were of course more concurrent sessions, covering a range of topics: from how environmental factors can influence development to cell shape and the cytoskeleton. At the evening poster session a rumour started going around that turtles could be spotted by the beach. And we must confess that we did go for a night walk on the beach, and that we could observe turtles laying their eggs. And even if a beach at the back of a strip of hotels cannot be really considered their natural habitat, after so many talks using turtles as subjects of study it was nice to see these beautiful animals in the ‘wild’!

 

The Node is at the ISDB in Cancun, and we are posting daily reports. In addition to this post you can read the report of day 1day 2 and day 3.

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Day 3 at the ISDB- a day of rest

Posted by , on 19 June 2013

Day 3 of the ISDB was really only half a day, with the only free afternoon/evening in the intense meeting program.

The first part of the morning featured two parallel sessions. One of the sessions was a roundtable discussion on how to succeed in science, and included a panel of very successful developmental biologists, sharing their advice and experience. The Node, however, attended the other session, an Education symposium sponsored by the SDB. It included a series of talks by different scientists who teach developmental biology to undergraduates, providing case studies and suggestions on how to engage students and effectively teach developmental biology concepts. One of the highlights of this session included the case study of Michael Barresi, who developed a semester-long practical class in which students learnt about embryonic development by testing the effects of crude oil in zebrafish development. Different students conducted proper scientific research on the effects on different aspects of development, and presented their results in a scientific symposium-style gathering at the end of the course. Some of the most enthusiastic students even continued the work during the summer, culminating with the publication of a paper. According with Michael Barresi, students cared about this project for two reasons: first, because they were conducting proper scientific research, and second because the project focused on a very topical issue that the students could relate to and feel engaged with (the big Gulf of Mexico oil spill had happened very recently before this course took place).

Indeed, the importance of teaching basic developmental biological concepts alongside real world, topical contextualisation of the importance of these studies, was a point stressed in this session. If the students care about it, they will be interested. Other useful suggestions included the use of texting, twitter or simple cards with A, B, C and D on them to ask questions to the whole class during lectures, making lectures dynamic and the students more engaged.

Following the education symposium there were several concurrent sessions, and the Node chose a session on emerging model organisms. One of the highlights of this session was a talk by Shigeru Kuratani, on the evolution of turtle body plans. His talk included some incredibly useful (and professional looking!) animations, which showed great 3D representations of the bones and muscles being studied, and how they are arranged in different organisms across evolution. They say a picture is worth a thousand words, and indeed it should not be underestimated how they can help understand complex scientific concepts. Another highlight from this session was Nanette Nascone-Yoder, who introduced the audience to the Budgett’s frog. The audience reacted both to the potentially useful properties of this animal for developmental biology studies (e.g. fast development rates, and big embryo size), but also to some more creepy features, such as how bigger tadpoles often eat their smaller siblings!

 

frog with qr codeNot a Budgett’s frog… but this little fellow has been spotted at a poster, alongside a QR code linking to more poster images!

During the afternoon off the Node was not resting- we have been interviewing some great scientists during this ISDB meeting, and the interviews will be posted on the Node in the near future. As for tomorrow… another full day of science ahead of us!

The Node is at the ISDB in Cancun, and we are posting daily reports. In addition to this post you can read the report of day 1 and of day 2.

 

 

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Day 2 at the ISDB- Not so much sun, but some great science

Posted by , on 18 June 2013

Let’s be honest- the first talk of the morning session in a conference is always badly attended, right? Well, not at the ISDB! In a clever move by the organisers, the very first speaker of day 2 was no other than Sir John Gurdon, and consequently the room was full. The chair of that plenary session was Eddy DeRobertis, who did a postdoc with John Gurdon. When introducing him, Eddy DeRobertis mentioned not only John Gurdon’s scientific achievements, and his recent Nobel Prize, but also his contributions to the scientific community: as president of the ISDB, his work at the Company of Biologists and Development, his travels around the world to teach and lecture. At Eddy DeRobertis’s request the whole room welcomed John Gurdon to the podium with a standing ovation, a memorable moment of this ISDB.

 

poster sessop

Day 2 also saw the beginning of the concurrent sessions. Each of the three simultaneous sessions has an overall theme that runs throughout the conference: ‘Molecules to Cells’, ‘Cells to Organs’ and ‘Organs to Organisms’, reflecting in a way the scale of the research presented in each session. The themes being covered each time vary however. For example, the ‘Organs to Organisms’ session in the morning focused on models of human diseases, while the one in the afternoon featured growth control. In addition to the plenary sessions, you can imagine the wide range of amazing research that is being covered at this meeting!

Janet Rossant's talk croppedAnother very nice moment of day 2 was the ISDB Harrison Medal Lecture. The Harrison Medal was previously known as the Harrison Prize, and was established in 1981 to recognize ‘scientists whose contributions have significantly advanced the field of developmental biology’. It is only awarded every four years, and hence to only a very select number of scientists. At this ISDB, the Harrison Prize became the Harrison Medal, and many of the previous winners, including Sir John Gurdon, Eddy DeRobertis, Masatoshi Takeichi and Elliot Meyerowitz, were present to receive their own, brand new, Harrison Medal. The winner of this year’s Harrison Medal was Janet Rossant. She gave a very entertaining talk- a fascinating overview of her career, which started as an undergraduate being taught by John Gurdon (science is such a small world!), but which also included some of the scientific questions that interest her the most at the moment. The Node will be interviewing Janet Rossant, so look out for a post about it in the near future.

The day concluded with another poster session. And for those of you that might have been jealous of the beautiful beach photo of the previous post, you might like to know that is not always openly sunny here in Cancun, and that there was also some rain!

 

 View from the conference centre in the morning:

sunny conference

View from the conference centre in the afternoon:

P6170123

 

The Node is at the ISDB in Cancun, and we are posting daily reports. You can read the report of day 1 here.

 

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ISSCR Annual Meeting – Day 4

Posted by , on 18 June 2013

Final day at the ISSCR. No posters. No concurrent sessions.  Just pure plenary all day. And indeed, the eclectic mix of talks provided a fitting end to 4 days of diverse science.

Ian Chambers kicked things off with an excellent talk describing the surprising recent finding by his laboratory that reduced levels of Oct4 actually improves self-renewal of mouse ES cells and reduces heterogeneity. Elena Cattaneo presented some beautiful stainings of early neural development in human fetuses, work that will surely interest many in the field, while Oliver Brustle gave a good overview of his laboratories work in modelling neural disease using neural stem cells derived from pluripotent stem cells. Benoit Bruneau described his labs impressive efforts to characterise the epigenome of cells at various stages during in vitro cardiac differentiation while Mitinori Saitou provided fascinating insights into the transcription factor control of primoridal germ cell specification. The morning talks were noteworthy not only for their high quality but also the diversity of experimental approaches used; including an assortment of pluripotent stem cell manipulations, reprogramming strategies, in vitro differentiation, -omics, and human in vivo studies.

The afternoon session talks were equally strong. Ken Zaret introduced characteristically meticulous work looking at pioneer transcription factors and the manipulation of chromatin states during both cellular differentiation and reprogramming. Kristin Baldwin presented her recent attempts to characterise the somatic mutational spectra using the formidable combination of somatic cell nuclear transfer (using terminally differentiated neurons) followed by ES cell derivation and whole genome sequencing! The theme of nuclear transfer then continued as one of the most eagerly awaited speakers – Shoukhrat Mitalipov – took to the stage. The recent publication of his derivation of cloned human ES cells created a major stir in the build up to the conference and many were eager to hear the full story of this major feat. They weren’t disappointed and were also treated to his thoughts on the applicability of this technique in the treatment of mitochondrial diseases. Next, Shinya Yamanka returned to the platform to present Marius Wernig with the ISSCR Outstanding Young Investigator Award. Wernig was then given the opportunity to present the work that merited this accolade, namely his groundbreaking studies on trans-differentiation. As well as giving the background to these landmark findings he presented more recent data in which he has taken an –omics approach to try and better characterise the process – including slavishly mapping the binding pattern of the ‘reprogramming’ factors in different cellular contexts. His talk provided an indication that not all his chosen transcription factors function in the same way during the transdifferentiation process, and he presented evidence of pioneer factor activity – an interesting link to Ken Zaret’s talk. Next, the topic shifted to regenerative medicine and included fascinating talks from Hans Snoeck, Graziella Pellegrini and Timothy Bertram. The later two presentations featured data generated following cell transplantation into patients – a goal of many in the field and an exciting way to finish the conference. However, there was just enough time for Eric Lander to provide an intriguing insight into his adventures unlocking the secrets of the human genome – admitting in the process that everything he taught his undergraduate students about the human genome in 2001 has now been proven wrong! I wonder what he taught this year that will suffer the same fate…?!

With a goodbye from Shinya Yamanaka, in his own inimitable style, the 2013 ISSCR came to an end. Although one final surprise came in the lobby where we were met by not only a free bar, but a free bar with some decent beers behind it too! Next year rolls on to Vancouver – although this blogger may well be hanging up his keyboard well before then!

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