Background

Horizontal gene transfer has played a major role in the evolution of bacteria, however, its importance in animals is less clear. Recent evidence suggests that insects have repeatedly acquired genes from symbiotic bacteria that have provided them with key adaptation that has led to their success. Examples of this include, the horizontal acquisition of genes for lignocellulose digestion that has led to herbivory in beetles, and toxin encoding genes that may underlie protection from natural enemies in aphids and vinegar flies. However, it is currently unclear how common the horizontal transfer of symbiont genes is across insect species, and in most cases whether the newly acquired genes actually provide insects with adaptive functions. It is crucial to understand the dynamics of horizontal gene transfer in insects as the acquisition of novel traits from microbes is transforming our view of how arthropods evolve. This is of particular important for insect pests as it may underlie their capacity to colonise new environments and resist efforts to control them.

The successful applicant will use several insect families (e.g. aphids, ants) as models to rigorously test hypotheses on horizontal gene transfer in the evolution of insects. This may include: using whole genome sequence data to explore horizontally acquired genes involved in defence and nutrition across phylogenetically diverse insect species; investigating gene expression profiles to determine gene activity; and performing functional assays to reveal whether horizontally acquired genes provide insects with novel adaptive functions.

• You will have access to large genetic databases, insect collections, in-house live organisms and cutting-edge research facilitties to fuel your investigation.
• You will gain experience of molecular/experimental biology, comparative/metagenomics, bioinformatics, and statistics (e.g. comparative phylogenetics).
• You will be encouraged to develop your own ideas and hypotheses.

Research Environment

The School of Biological and Chemical Sciences at Queen Mary is one of the UK’s elite research centres, according to the 2014 Research Excellence Framework (REF). We offer a multi-disciplinary research environment and have approximately 160 PhD students working on projects in the biological, chemical and psychological sciences. Our students have access to a variety of research facilities supported by experienced staff, as well as a range of student support services.

The successful applicant will enter a vibrant research environment, under the supervision of Dr Lee Henry and co-supervision of Prof Richard Nichols and Dr Chema Martin. The Henry lab is well equipped to carry out the proposed research with world-class facilities in genomics and molecular biology. The student will also have access to ample funds to facilitate the research through Dr. Henry’s external funding (~£1 million in the past ten years). Dr. Henry has an outstanding record with student supervision with a focus on students generating high impact first authorship publications.

Dr Lee Henry is a Senior Lecturer (UK equivalent to Associate Professor) in Biology. For details see: https://www.qmul.ac.uk/sbcs/staff/leehenry.html

Training and Development

Our PhD students become part of Queen Mary’s Doctoral College which provides training and development opportunities, advice on funding, and financial support for research. Our students also have access to a Researcher Development Programme designed to help recognise and develop key skills and attributes needed to effectively manage research, and to prepare and plan for the next stages of their career.

The PhD student will have access to large genetic database, field collected insect samples and live in-house organisms. They will gain experience in molecular techniques, bioinformatics, experimental/field biology, and statistics (e.g. GLMM, comparative phylogenetics).

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By Mai P. Tran and Kimberly L. Cooper

“It’s the cutest rodent I have ever seen, even cuter than a cuddly hamster, and it would be fun doing a rotation for the opportunity to work with this animal.” That was my thought, as a first-year graduate student, when I first heard Kim present her research on the development of digit loss in the jerboa. I was completely hooked by the jerboa’s fascinating biology, and the Cooper lab became my second family. I had no idea then how working with the challenges of a non-traditional species would also mature my scientific thinking.

The vertebrate form is incredibly diverse, as evident not only in the body shapes and sizes but also in the limbs. For example, bats have elaborated forelimbs capable of flight while snakes have lost all four limbs. Such diversity has long inspired scientists and provided opportunities to investigate both developmental and evolutionary processes. To understand the developmental programs that explain limb diversity, the Cooper lab uses the bipedal jerboa (Figure 1), a close relative of the mouse with very different hindlimbs. The jerboa hindlimb has lost the first and fifth toes, fused the three central metatarsals into a single bone, disproportionately elongated the foot, and lost all intrinsic foot muscles. These modifications of the limb form allow the jerboa to jump high and rapidly change orientation, an advantage to escape predators in its desert environment with sparse vegetation. The jerboa and mouse have similar forelimb morphology, which serves as a great internal control for comparative anatomy and genomics to identify potential mechanisms specific to hindlimb divergence.

Our recent work, Tran et al. eLife 2019, showed that the normal developmental process of muscle loss in the jerboa is similar to pathological muscle atrophy. In this post, I will elaborate on our findings, sprinkled with scientific speculation, and discuss the experimental challenges that we encountered during our exploration of the fascinating biology of this non-traditional laboratory animal model.

In 1979, Susan Berman, then a graduate student at the University of Pittsburgh who later became faculty at College of the Holy Cross, published a description of the hindlimb skeletal and muscular anatomy of many bipedal rodents including several species of jerboas¹. When I started my work in 2014, we knew from her work that the adult jerboa has no ‘intrinsic’ muscles within its feet that spread and draw together toes of more basally branching species. Instead, most jerboa species have robust tendons in each location that we presume stabilize the ankle when animals jump and land with great force. However, we knew nothing of the developmental process leading to the evolutionary loss of these muscles.

Jimmy Hu, a graduate student with Cliff Tabin while Kim was a postdoc, had recently shown that limb myoblasts require reception of Shh signal to complete migration into the distal limb, as Smoothened receptor loss-of-function in mouse muscle causes a complete absence of muscles in the hands and feet². A similar failure of muscle progenitor cells to populate the distal jerboa hindlimb seemed a simple explanation for the adult phenotype. We thought this project might be ‘low-hanging fruit’ for our nascent lab. We were therefore surprised to find that not only do myoblasts reach the foot, they fuse, differentiate, and form sarcomeres before rapidly disappearing in the first week after birth.

We next assumed the muscles must have died. Programmed cell death eliminates ‘extra’ tissue to shape developing structures in many contexts, including the process that carves interdigital webbing away from the fingers and toes like pulling dough away from the edges of a cookie cutter. Convinced this must also be the case for developing muscle that disappears so quickly, I performed multiple assays at all stages of muscle loss fruitlessly searching for signs of compromised cell membrane, fragmented DNA, or immune cell recruitment. I’ve also looked at thousands of muscle cells by transmission electron microscopy, a classic approach used to describe programmed cell death before there were kits and antibodies. Although myofibers don’t look entirely normal (they have large lipid droplets and vacuoles, and the sarcomere disintegrates), the plasma membrane appears intact, the nucleus appears normal, and there is no immune cell infiltration suggesting these cells do not die. I thought that I must be doing something wrong, but I had included all the positive and negative controls I could think of and covered all the stages of muscle loss. The more data I amassed suggesting this simple hypothesis was invalid, the more frustrated I grew. In contrast, my advisor became more and more excited.

If muscle cells did not seem to die, what was their fate? We considered the possibility that muscle cells switch identity, which would explain the disappearance of muscle proteins. The robust tendon in the adult and the appearance of collagen-producing cells surrounding odd extra-cellular aggregates of muscle filament protein led us to hypothesize that perhaps muscle cells could become some type of collagen-producing connective tissue fibroblast.

To definitively show that muscle cells become another cell type, or that they die by an unusual mechanism we were unable to detect by common assays, we would need to lineage label jerboa foot muscle cells and follow their existence over time. For over three years, we tried viral infection and plasmid electroporation approaches using multiple ‘muscle-specific’ promoters to lineage trace muscle cells using a Cre/lox reporter system, but none was successful. There were two technical complications hindering our ability to successfully lineage label muscle cells, discussed briefly in the response to eLife reviewers; we were unable to label a sufficient number of cells in neonatal mouse or jerboa feet, and none of the promoters specifically labeled muscle in either species. We hope that our ongoing work utilizing single-nucleus sequencing of muscle and surrounding tissue can help shed light on the fate of muscle cells by reconstructing a ‘virtual lineage’ using global similarities in the transcriptome of heterogeneous cell types³. Though not as definitive as a lineage tracing experiment, this might provide valuable insight into the most likely fate of these cells.

During those years of trying to determine the fate of the muscle, I also took a parallel approach to learn more about the developmental trajectory of jerboa foot muscles and to identify the earliest signs of impending loss. I found that muscle sarcomeres, though assembled, never fully mature to align with one another and instead became disorganized into pools of filaments. We were curious to know if the process of muscle disassembly happened all at once or in some orderly fashion. My observation that Desmin was the first protein to lose its striated pattern was our first break in this challenging project, since this was similar to what was observed in conditions of skeletal muscle atrophy. Even though atrophy is typically considered a response to disease or injury, might it be possible that the ‘normal’ developmental process of jerboa foot muscle loss is similar to atrophy? Indeed, expression of genes associated with pathological muscle atrophy, called “atrogenes,” was significantly higher in jerboa foot muscles than other ‘typical-developing’ muscles.

In 1883, Daniel John Cunningham, a Scottish physician, zoologist, and anatomist, reported the presence of intrinsic foot muscles in fetal horses, deer, and oxen that lack these muscles as adults. Cunningham observed fat and fibrotic tissue infiltration that replaced the disappearing foot muscles4. When we re-read the work of Cunningham as we prepared our own manuscript, we realized that Cunningham had left a hint we weren’t initially prepared to appreciate when he wrote “it is an instance of pathological change assisting a morphological process”⁴. Now aided with molecular and genetic tools, we show that the natural process of muscle loss in jerboa does indeed share molecular characteristics with pathological atrophy. If we look carefully at other ‘non-traditional species’, we may find that foot muscle atrophy in the jerboa is just one of many cellular responses associated with injury or disease in humans that is utilized in the normal development and physiology of other species.

While it is tempting to think that we might prove a cause and effect relationship by knocking down expression of the atrogenes to rescue jerboa foot muscle, it is important to consider differences between macroevolutionary phenotypes and mechanistically simpler phenotypes that are more typically studied in the lab. A comparison of the foot anatomy of living jerboas and their nearest relatives reveals that a subset of muscles was lost about 20 million years ago gradually leading to absence of all foot muscle in more recently derived jerboas. We therefore expect that multiple genetic changes contribute to the mechanism of muscle cell loss, perhaps including mutations that primarily affect integrated tissues like the skeleton and connective tissues. Although multiple genes responsible for evolutionary phenotypes can be identified by quantitative trait locus (QTL) analyses, the approach requires that species with different morphologies produce fertile hybrids. The jerboa lineage diversified too long ago for such mattings to be possible. Instead, we are currently applying interspecies differential RNA-Seq of mouse and jerboa foot and forearm muscles to identify genes with expression differences that are specifically associated with jerboa foot muscle loss. In addition to building a comprehensive molecular profile of this process, these data provide an opportunity to identify genes not previously known to influence muscle maturation and maintenance.

Thinking about the big picture of my research brought me to the realization that evo devo isn’t simply the study of individual phenomena in unusual critters. All deviations from ‘typical development’, both adaptive and maladaptive, provide insight into unifying principles. Studies of non-traditional species, while they can be technically challenging, will not only reveal the mechanisms of evolutionary malleability, but also advance our understanding of fundamental biological processes.

References

1. Berman, S. L. Convergent evolution in the hindlimb of bipedal rodents. Journal of Zoological Systematics and Evolutionary Research23, 59–77 (1985).
2. Hu, J. K.-H., McGlinn, E., Harfe, B. D., Kardon, G. & Tabin, C. J. Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes & development26, 2088–102 (2012).
3. Trapnell, C. et al. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nature Biotechnology 32, 381–386 (2014).
4. Cunningham, D. J. The Development of the Suspensory Ligament of the Fetlock in the Foetal Horse, Ox, Roe-Deer, and Sambre-Deer. Journal of anatomy and physiology18, i1-12 (1883).

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Background

The development of an entire animal from a single totipotent cell – the zygote – is arguably one of the most fascinating processes in Nature. Strikingly, how this process is regulated at the lower levels of biological complexity, such as at the level of the genome and its regulation during the early phases of development, is still poorly understood. Moreover, our understanding relies on what is known for only a handful of species, such as mammals and flies. To solve this major knowledge gap, my lab is establishing annelid embryos as experimental systems to understand the genomic regulation of early animal development. Annelid embryos are unique in that they exhibit interspecific variation in the way the zygote gives rises to the major progenitor cells during early cleavage. What are the epigenetic mechanisms controlling annelid development? How do these mechanisms generate variability among species? How can annelid development inform us of the fundamental principles of animal embryogenesis?

• In this project you will rigorously answer these questions combining state-of-the-art experimental and computation approaches.
• You will have access to large genomic databases, and in-house live organisms to fuel your investigation.
• You will gain experience of developmental biology and molecular techniques (gene expression analyses, epigenomics), bioinformatics (pipelines to analyse ATAC-seq, ChIP-seq and HiC), and statistics.
• You will be encouraged to develop your own ideas and hypotheses.

This is an ERC-Starting Grant PhD funded position. The student will become part of Queen Mary’s Doctoral College, which provides training and development opportunities and financial support for research. The student will also have access to a Researcher Development Programme designed to help recognise and develop key skills and attributes needed to effectively manage research, and to prepare and plan for the next stages of their career.

Skills preferred

In a multidisciplinary project like this, candidates are unlikely to have a background in all disciplines involved.  The most important qualification is motivation, enthusiasm and that the project appeals to you. However, previous computational experience would be a plus. We can envisage strong candidates coming through a variety of routes including:

– practical molecular biology

– developmental and cell biology

– computational biology

To apply, students should have a 1^st class degree or have received a MSc in a relevant field (i.e. molecular biology, genetics, developmental and cell biology, bioinformatics) or are about to finish their MSc.

For informal requests, do not hesitate to contact Dr Chema Martin at chema.martin@qmul.ac.uk or visit the lab website at martinduranlab.com

Apply via: https://www.qmul.ac.uk/sbcs/postgraduate/phd-programmes/projects/display-title-760193-en.html

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A new year is a good opportunity to look back on the one just passed, and whether you’re a Node regular or new to us, this selection of our 2019 highlights will hopefully be of interest, particularly if you’re interested in contributing something and looking for inspiration. We are a community site for and by developmental biologists – it’s free and easy to post, just sign up here. And if you don’t already, be sure to follow us on Twitter and Facebook. Happy new year!

Top 5 most-read posts published in 2019

Congratulations to Joachim Goedhart (twice, and for the second year in a row) and Shinichi Morita for making our top 5 most-read articles this year.

1. the Node Calendar competition – vote here, vote now! (look out for a new cover competition in the summer)
2. User-friendly p-values by Joachim Goedhart
3. Data Visualization with Flying Colors by Joachim Goedhart
4. A day in the life of a Kabuto-mushi (rhinoceros beetle) lab by Shinichi Morita (Dec 2018 but most reads in 2019)
5. December in preprints (our monthly preprint list remains popular)

Behind the paper stories

We love to hear the stories behind the latest papers – these might be personal recollections or more data-focused, and can take advantage of our informality and lack of word limit. Here’s ten of my favourites from 2019:

• Collaboration: All the things we cannot see (alone) by Miriam Rosenberg and Suparna Ray
• Plant stem cells strive towards equality by George Bassel and Iain Johnston
• A domino effect on brain developmental evolution by Jorge Torres-Paz
• A biology-modeling crosstalk to uncover feather pattern evolution by Richard Bailleul, Jonathan Touboul and Marie Manceau
• “My balance comes from instability” thought Herzog (Saul Bellow, Herzog)

  

  by Zelha Nil

• Down from the crest to clear the dead by Yunlu Zhu and Sarah Kucenas
• Inflate your embryo by Julien Dumortier and Mathieu Le Verge-Serandour
• Discovering the Genetic Basis of Mimetic Color Diversity in Bumble Bees by Heather Hines
• Forces maintain order between cells by Antoine Fruleux and Arezki Boudaoud
• Beyond morphogen signaling by Dörthe Jülich & Scott Holley

Lab life

We had two new entries to our ‘Day in the life of an X lab’ series, which showcases the diversity of model systems used in developmental biology. Kohei Oguchi from Tokyo University told us all about termites, while Sandra Treffkorn from the University of Kassel in Germany versed us in velvet worms (onychophorans).

We’re now up to 42 in the series (Arabidopsis, Ascidian, Axolotl, Bat, Butterfly, C. elegans, Capitella, Chicken, Cnidaria, Colonial tunicate, Coral, Cricket, Ctenophore, Drosophila, ES cells, Gar, Gecko, Honeybee, Hydractinia, Lamprey, Larval-microbe, Maize, Marchantia, Mayfly, Moss, Mouse, Oikopleura, Onychophoran, Parhyale, Planaria, Platynereis, Rhino beetle, Sea urchin,
Shark, Siphonophore, Skate, Spider, Sponge, Termite, Turtle, Xenopus, Zebrafish). Is your model missing? Get in touch.

Art and science

In March we posted a fascinating account from an artist (Gemma Anderson, University of Exeter) and a cultural historian of science (Janina Wellmann, MECS, Leuphana University Lüneburg) who visited the EMBL. Their outsider perspective on how science is done is well worth the read.

In October we interviewed Annabel Ebbing (the Hubrecht Institute in The Netherlands), first author of a Development paper on C. elegans neuroblast migration. She designed a beautiful piece of worm art that made Development’s cover – we heard about what science and art meant to her.

Science on tour

We published many meeting, workshop and course reports this year:

• Santa Cruz Developmental Biology Conference by me and Jordan Ward
• Woods Hole Embryology Course x3 by Andrea Attardi, Aastha Garde, Anneke Kakebeen
• 1st Crick-Beddington Developmental Biology Symposium in London x2 by Vicki Metzis and Alex Eve
• Evo-chromo: towards an integrative approach of chromatin dynamics across eukaryotes in Wiston House by Alexander Blackwell and James Gahan
• Polyploidy in Organ Development, Repair, and Disease in Bar Harbor by Adrienne Roeder
• Young Embryologist Network in London by Alex Eve
• Chromatin-Based

  

  Regulation of Development in Witson House x2 by Abby Buchwalter and Martyna Lukoseviciute

• New Frontiers in the Brain: Unexpected Roles of the Choroid Plexus-Cerebrospinal Fluid System in Health and Disease in Wiston House by Aida Rodrigo Albors, Laura Pellegrini and Neil Dani
• Understanding Birth Defects in the Genomic Age in Wiston House by Ashish Deshwar and Nicole Edwards
• Latin American Society for Developmental Biology Meeting in Buenos Aires by me

We also heard from two recipients of Travelling Fellowships from the Company of Biologists: Miquel Sendra, who went from Madrid to Washington, and Estefanía Sánchez-Vásquez, who went from Buenos Aires to Pasadena.

The practice and promotion of developmental biology

Regular Node contributor Joachim Goedhart posted four commentaries on user-friendly p-values, experimenting with non-anonymous peer review, barrier-free use of colors in images and graphs, and data normalization. We also heard more about data visualisation from Helena Jambor in her post on non-zero baselines.

We posted three perspectives on the effects of preprints on research. Katherine Brown reported from the European Developmental Biology Conference 2019 Preprint Workshop (earlier in the year, Katherine also shared her thoughts on publishing Drosophila research). Mate Palfy and Gautam Dey considered whether preprints and science news can coexist. Finally, a team of preLighters responded to a meta analysis on bioRxiv preprints.

Melissa McCartney explained why introducing biology undergrads to the primary literature is valuable, and we heard a Chinese perspective on learning developmental biology from Guojun Sheng and students.

Things to look out for in 2020

January The launch of the Node Network, a global directory of developmental and stem cell biologists. You may have filled out our survey in the summer – the site is nearly there now and we’d love as many people as possible to sign up for it.

March(ish) We will run a community survey all about the Node. What can we do better in the coming years? What type of content do you want to see more (or less) of? What are we missing? Look our for our survey early in the year.

April Cast your mind back to April 1st, 2010 – Gordon Brown was UK Prime Minister, Clash of the Titans (eh?) topped the cinema charts, atmospheric carbon dioxide was 389ppm…and the Node was launched, publishing its first post (the first of 2403 and counting). Ten years on and it’ll be a good time to reflect on the past and look forward to the future. We’ll probably have some cake in the office and metaphorical cake on Twitter!

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Department/Location: Wellcome Trust-Medical Research Council Stem Cell Institute

Salary: £32,816-£40,322

Reference: PS21993

Category: Research

Published: 6^th January, Closing date: 5^th February 2020

A postdoctoral Research Associate position is currently available for an individual to work in the laboratory of Prof. Anna Philpott within the Cambridge Stem Cell Institute (https://www.stemcells.cam.ac.uk/research/pis/philpott). The Philpott lab has broad interests in understanding the fundamental mechanisms that determine cell fate choice and differentiation during embryonic development and in cancers, as well as how these processes are co-ordinated with cell cycle progression.

The successful candidate will undertake a project focused around transcriptional regulation of lineage fidelity during fate specification and differentiation of mouse embryonic stem cells, focusing on uncovering epigenetic and co-factor-dependent mechanisms underlying these processes. There is also an opportunity to work on parallel mechanisms of fate specification and differentiation in Xenopus embryos.  Within the laboratory, we use several experimental systems including mammalian embryonic stem cells, cancer cell culture, organoid systems as well as embryos of the frog Xenopus laevis. We use many techniques including genome-wide analysis of gene expression in single and multiple cells, chromatin binding and accessibility studies and crispr genome editing, alongside diverse biochemical approaches.

The successful candidate will have a PhD, considerable experience in stem cell biology, epigenetics, molecular biology, developmental biology, or a similar field, and a proven track record in scientific publication. Prior experience in mammalian cell culture is essential. Experience of epigenetics and/or transcriptional regulation are essential, while experience of genome-wide transcriptional analysis, and in particular analysis at the single cell level, would also be an advantage. Applicants must display an ability to undertake project management, work within a multi-disciplinary team environment, have good presentation and communication skills and the ability to contribute to an environment supporting researchers at all stages of their careers.

The Wellcome – MRC Cambridge Stem Cell Institute (CSCI) is a world-leading centre for stem cell research with the mission to transform human health through a deep understanding of stem cell biology. https://www.stemcells.cam.ac.uk/ . CSCI moved to the brand new, state of the art Jeffrey Cheah Biomedical Centre building on the Cambridge Biomedical Campus in summer 2019. The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.

Application via: http://www.jobs.cam.ac.uk/job/24656/

Informal enquiries should be directed to Prof. Anna Philpott, ap113@cam.ac.uk

Fixed-term: The funds for this post are available for 3 years in the first instance.

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A 3-years-PhD position is available at the Chédotal Lab at the Vision Institute in Paris.
The goal of the PhD project will be to describe the organization of the developing human eye and characterize molecular and cellular mechanisms underlying human retinogenesis. We propose to revisit eye formation combining modern and innovative single-cell molecular and 3D imaging approaches on human embryo/fetal eye samples. Data from light sheet fluorescent microscopy (LSFM), spatial transcriptomics and in situ sequencing will be incorporated into one scaffold to provide a comprehensive 3D map of human retinogenesis. The project will provide significant and detailed insights into human ophthalmology, generating the first reference map of human retina development. It will further contribute to the Human Cell Atlas initiative. The thesis will be directed by Dr Alain Chédotal.
An experience in bioinformatics and transcriptomics or 3D imaging would be a plus but is not required.
Candidates should hold a diploma or a master’s degree in Neuroscience, Developmental Biology, Microscopy or Bioinformatics
They may send their application (in a PDF-format) to Dr Alain Chédotal (alain.chedotal@inserm.fr), including a CV and contact information for two references.

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Welcome to our monthly trawl for developmental biology (and related) preprints. 

Here’s the last cache of preprints of 2019 – happy preprinting in 2020! They were hosted on bioRxiv and arXiv. Let us know if we missed anything. Use these links to get to the section you want:

Developmental biology

| Patterning & signalling

| Morphogenesis & mechanics

| Genes & genomes

| Stem cells, regeneration & disease modelling

| Plant development

Evo-devo & evo

Cell biology

Modelling

Tools & resources

Research practice & education

Why not…

Developmental biology

| Patterning & signalling

Interdependent regulation of stereotyped and stochastic photoreceptor fates in the fly eye
Adam C. Miller, Elizabeth Urban, Eric L. Lyons, Tory G. Herman, Robert J. Johnston Jr.

Constraints and limitations on the transcriptional response downstream of the Bicoid morphogen gradient
Huy Tran, Aleksandra M. Walczak, Nathalie Dostatni

The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis
Zhe Yang, Hongcun Zhu, KaKit Kong, Jiayi Chen, Xiaxuan Wu, Peiyao Li, Jialong Jiang, Jingchao Zhao, Feng Liu

FGFR/Heartless and Smog interact synergistically to negatively regulate Fog mediated GPCR signaling
Kumari Shweta, Anagha Basargekar, Anuradha Ratnaparkhi

Modulation of Yorkie activity by alternative splicing is required for developmental stability
Diwas Srivastava, Marion de Toledo, Laurent Manchon, Jamal Tazi, François Juge

Neuropeptide F receptor acts in the Drosophila prothoracic gland to regulate growth and developmental timing
Jade R. Kannangara, Michelle A. Henstridge, Linda M. Parsons, Shu Kondo, Christen K. Mirth, Coral G. Warr

Endocycles support tissue growth and regeneration of the adult Drosophila accessory gland
Allison M. Box, Samuel Jaimian Church, David Hayes, Shyama Nandakumar, Russell S. Taichman, Laura Buttitta

Compartment and cell type-specific hypoxia responses in the developing Drosophila brain
Martin Baccino-Calace, Daniel Prieto, Rafael Cantera, Boris Egger

Presynaptic developmental plasticity allows robust sparse wiring of the Drosophila mushroom body
Najia A. Elkahlah, Jackson A. Rogow, Maria Ahmed, E. Josephine Clowney

Pegasus, a small extracellular peptide regulating the short-range diffusion of Wingless
Emile G Magny, Jose I Pueyo, Sarah A Bishop, Daniel Aguilar-Hidalgo, Juan Pablo Couso

An insulin, AMPK, and steroid hormone-mediated metabolic switch regulates the transition between growth and diapause in C. elegans
Sider Penkov, Bharath Kumar Raghuraman, Cihan Erkut, Jana Oertel, Roberta Galli, Eduardo Jacobo Miranda Ackerman, Daniela Vorkel, Jean-Marc Verbavatz, Edmund Koch, Karim Fahmy, Andrej Shevchenko, Teymuras V. Kurzchalia

The pattern of Nodal morphogen signaling is shaped by co-receptor expression
Nathan D. Lord, Adam N. Carte, Philip B. Abitua, Alexander F. Schier

Enhanced canonical Wnt signaling during early zebrafish development perturbs the interaction of cardiac mesoderm and pharyngeal endoderm and causes thyroid specification defects
Isabelle Vandernoot, Benoît Haerlingen, Achim Trubiroha, Pierre Gillotay, Véronique Janssens, Robert Opitz, Sabine Costagliola

BACH family members regulate angiogenesis and lymphangiogenesis by modulating VEGFC expression
Batya Cohen, Hanoch Tempelhof, Tal Raz, Roni Oren, Julian Nicenboim, Filip Bochner, Ron Even, Adam Jelinski, Raya Eilam, Shifra Ben-Dor, Yoseph Adaddi, Ofra Golani, Shlomi Lazar, Karina Yaniv, Michal Neeman

Klf9 is a key feedforward regulator of the transcriptomic response to glucocorticoid receptor activity
Ian Gans, Ellen I. Hartig, Shusen Zhu, Andrea R. Tilden, Lucie Hutchins, Nathaniel Maki, Joel H. Graber, James A. Coffman

Copper Induces Zebrafish Central Neural System Myelin Defects: the Regulatory Mechanisms in Wnt/Notch-hoxb5b Signaling and Underlying DNA Methylation
Ting Zhang, PengPeng Guan, Guang Zhao, YaPing Fang, Hui Fu, Jian-Fang Gui, GuoLiang Li, Jing-Xia Liu

Cavefish Increase Red Blood Cell Development and Reprogram Metabolism as Adaptations to Environmental Hypoxia
Corine M. van der Weele, William R. Jeffery

Species-specific developmental timing is associated with global differences in protein stability in mouse and human
Teresa Rayon, Despina Stamataki, Ruben Perez-Carrasco, Lorena Garcia-Perez, Christopher Barrington, Manuela Melchionda, Katherine Exelby, Victor Tybulewicz, Elizabeth M. C. Fisher, James Briscoe

The dynamics of oligodendrocyte generation: how distinct is the mouse from the human?
David G Gonsalvez, Georgina A Craig, Darragh M Walsh, Barry D Hughes, Rhiannon J Wood, Sang Won Yoo, Simon S Murray, Junhua Xiao

Growth factor-mediated coupling between lineage size and cell fate choice underlies robustness of mammalian development
Nestor Saiz, Laura Mora-Bitria, Shahadat Rahman, Hannah George, Jeremy P Herder, Jordi Garcia-Ojalvo, Anna-Katerina Hadjantonakis

FGF9 and FGF10 use distinct signaling pathways to direct lung epithelial specification and branching
Yongjun Yin, David M. Ornitz

Role of SHIP2 in cell repulsion regulated by Eph receptor and ephrin signaling
Tim G. Ashlin, Zhonglin Wu, Qiling Xu, David G. Wilkinson

Nwd1 regulates neuronal differentiation and migration through purinosome formation in the developing cerebral cortex
Seiya Yamada, Ayaka Sato, Shin-ichi Sakakibara

Hominini-Specific Regulation of CBLN2 Increases Prefrontal Synaptogenesis
Mikihito Shibata, Kartik Pattabiraman, Sydney K. Muchnik, Nenad Sestan

Regulation of Prefrontal Patterning, Connectivity and Synaptogenesis by Retinoic Acid
Mikihito Shibata, Kartik Pattabiraman, Belen Lorente-Galdos, David Andrijevic, Xiaojun Xing, Andre M. M. Sousa, Gabriel Santpere, Nenad Sestan

Microglia depletion disrupts normal functional development of adult-born neurons in the olfactory bulb
Jenelle Wallace, Julia Lord, Lasse Dissing-Olesen, Beth Stevens, Venkatesh Murthy

Environmental Oxygen Regulates Astrocyte Proliferation to Guide Angiogenesis during Retinal Development
Robin M Perelli, Matthew L O’Sullivan, Samantha Zarnick, Jeremy N Kay

Circuit-specific dendritic development in the piriform cortex
Laura Moreno-Velasquez, Malte Kaehne, Hung Lo, Stephen Lenzi, Jörg Breustedt, Dietmar Schmitz, Sten Rüdiger, Friedrich W. Johenning

Oxytocin shapes spontaneous activity patterns in the developing visual cortex by activating somatostatin interneurons
Paloma P Maldonado, Alvaro Nuno-Perez, Jan Kirchner, Elizabeth Hammock, Julijana Gjorgjieva, Christian Lohmann

A SMAD1/5-YAP signaling module drives radial glial cell expansion and growth of the developing cerebral cortex
Sonia Najas, Isabel Pijuan, Anna Esteve-Codina, Susana Usieto, Juan D. Martinez, An Zwijsen, Maria L. Arbonés, Elisa Martí, Gwenvael Le Dréau

A switch in cilia-mediated Hedgehog signaling controls muscle stem cell quiescence and cell cycle progression
Sara Betania Cruz-Migoni, Kamalliawati Mohd Imran, Aysha Wahid, Oisharja Rahman, James Briscoe, Anne-Gaëlle Borycki

Association of Sonic Hedgehog with the Extracellular Matrix Requires its Putative Zinc-Peptidase Activity
Carina Jägers, Henk Roelink

Moonlighting α-PheRS connects JAK/STAT with Notch signaling for intestinal homeostasis
Manh Tin Ho, Jiongming Lu, Beat Suter

Niacin Stimulates Mammary Gland Development in Pubertal Mice through Activation of the AKT/mTOR and ERK1/2 Signaling Pathways
Yu Cao, Juxiong Liu, Lijun Ma, Qing Zhang, Jiaxin Wang, Wenjin Guo, Yanwei Li, Ji Cheng, Shoupeng Fu

Wwc2 is a novel mitotic/meiotic cell-cycle regulator and cell fate related gene, during preimplantation mouse embryo development and oogenesis
Giorgio Virnicchi, Pablo Bora, Lenka Gahurová, Andrej Šušor, Alexander W. Bruce

| Morphogenesis & mechanics

Cytoplasmic streaming drifts the polarity cue and specifies the cell polarity in Caenorhabditis elegans zygotes
Kenji Kimura, Akatsuki Kimura

Geometric cues stabilise long-axis polarisation of PAR protein patterns in C. elegans
Raphaela Geßele, Jacob Halatek, Laeschkir Würthner, Erwin Frey

RhoGAP RGA-8 supports morphogenesis in C. elegans by polarizing epithelia through CDC-42
Hamidah Raduwan, Shashikala Sasidharan, Luigy Cordova Burgos, Andre G. Wallace, Martha C. Soto

Multiple requirements for Rab GTPases in the development of Drosophila tracheal dorsal branches and terminal cells
Benedikt T. Best, Maria Leptin

broad controls leg imaginal disc morphogenesis in Drosophila via regulation of cell shape changes and remodeling of extracellular matrix
Clinton Rice, Stuart Macdonald, Xiaochen Wang, Robert E Ward IV

A polarized nucleus-cytoskeleton-ECM connection controls collective migration and cardioblasts number in Drosophila
C Dondi, B Bertin, JP Da Ponte, I Wojtowicz, K Jagla, G Junion

Apical Constriction Reversal upon Mitotic Entry Underlies Different Morphogenetic Outcomes of Cell Division
Clint S. Ko, Prateek Kalakuntla, Adam C. Martin

Lgl cortical dynamics are independent of binding to the Scrib-Dlg complex but require Dlg-dependent restriction of aPKC
Guilherme Ventura, Sofia Moreira, André Barros-Carvalho, Mariana Osswald, Eurico Morais-de-Sá

Distinct activities of Scrib module proteins organize epithelial polarity
Mark J. Khoury, David Bilder

Competition between kinesin-1 and myosin-V define Drosophila posterior determination
Wen Lu, Margot Lakonishok, Rong Liu, Neil Billington, Ashley Rich, Michael Glotzer, James R. Sellers, Vladimir I. Gelfand

The emergent Yo-yo movement of nuclei driven by collective cytoskeletal remodeling in pseudo-synchronous mitotic cycles
Zhiyi Lv, Jan Rosenbaum, Stephan Mohr, Xiaozhu Zhang, Deqing Kong, Helen Preiß, Sebastian Kruss, Karen Alim, Timo Aspelmeier, Jörg Großhans

Fibronectin-dependent tissue mechanics regulate the translation of segmentation clock oscillations into periodic somite formation
Patrícia Gomes de Almeida, Pedro Rifes, Ana Patrícia Martins-Jesus, Gonçalo G. Pinheiro, Raquel P. Andrade, Sólveig Thorsteinsdóttir

Position-sensing established during compaction dictates cell fate in the mammalian embryo
Christophe Royer, Karolis Leonavicius, Annemarie Kip, Deborah Fortin, Kirtirupa Nandi, Anna Vincent, Celine Jones, Tim Child, Kevin Coward, Chris Graham, Shankar Srinivas

Ectoderm to mesoderm transition by downregulation of actomyosin contractility
Leily Kashkooli, David Rozema, Lina Espejo-Ramirez, Paul Lasko, François Fagotto

Effects of nectin-3 misexpression on dendritic spine density during the postnatal development of layer 2/3 cortical neurons
Johanna Tomorsky, Philip R. L. Parker, Chris Q. Doe, Cristopher M. Niell

MLL4 is required for the first embryonic collective cell migration whereas MLL3 is not required until birth
Deepthi Ashokkumar, Qinyu Zhang, Christian Much, Anita S. Bledau, Jun Fu, Konstantinos Anastassiadis, A. Francis Stewart, Andrea Kranz

Thrombospondin-1 Promotes Circuit-Specific Synapse Formation via β1-Integrin
Sehwon Koh, Suva Roy, Oznur Eroglu, Samuel Strader, William J. Chen, Jeremy N. Kay, Greg D. Field, Cagla Eroglu

Laminin alpha 5 is Necessary for Mammary Epithelial Growth and Function by Maintaining Luminal Epithelial Cell Identity
Johanna I Englund, Hanne Cojoc, Leander Blaas, Alexandra Ritchie, Nalle Pentinmikko, Julia Döhla, Pauliina Munne, Manuel Patarroyo, Juha Klefström, Johanna Ivaska, Pekka Katajisto

IRSp53 shapes the plasma membrane and controls polarized transport at the nascent lumen during epithelial morphogenesis
Sara Bisi, Syed Abrar Rizvi, Stefano Marchesi, Davide Carra, Galina V. Beznoussenko, Ines Ferrara, Gianluca Deflorian, Alexander Mironov, Giovanni Bertalot, Federica Pisati, Amanda Oldani, Angela Cattaneo, Salvatore Pece, Giuseppe Viale, Angela Bachi, Claudio Tripodo, Giorgio Scita, Andrea Disanza

Polar pattern formation induced by contact following locomotion in a multicellular system
Masayuki Hayakawa, Tetsuya Hiraiwa, Yuko Wada, Hidekazu Kuwayama, Tatsuo Shibata

A Nodal/Eph signalling relay drives the transition from apical constriction to apico-basal shortening in ascidian endoderm invagination
Ulla-Maj Fiuza, Takefumi Negishi, Alice Rouan, Hitoyoshi Yasuo, Patrick Lemaire

| Genes & genomes

Nine-banded armadillo quadruplets from Balloiuz, et al. 

The transcriptional legacy of developmental stochasticity
Sara Ballouz, Maria T. Pena, Frank M. Knight, Linda B. Adams, Jesse A. Gillis

Changing the Waddington landscape to control mesendoderm competence
James R. Valcourt, Roya Huang, Sharmistha Kundu, Divya Venkatasubramanian, Robert E. Kingston, Sharad Ramanathan

Zebrafish embryonic tissue differentiation is marked by concurrent cell cycle dynamic and gene promoter regulatory changes
Joseph W Wragg, Leonie Roos, Dunja Vucenovic, Nevena Cvetesic, Boris Lenhard, Ferenc Müller

HOX paralogs selectively convert binding of ubiquitous transcription factors into tissue-specific patterns of enhancer activation
Laure Bridoux, Peyman Zarrineh, Joshua Mallen, Mike Phuycharoen, Victor Latorre, Frank Ladam, Marta Losa, Charles Sagerstrom, Kimberley A. Mace, Magnus Rattray, Nicoletta Bobola

Hox binding specificity is directed by DNA sequence preferences and differential abilities to engage inaccessible chromatin
Milica Bulajić, Divyanshi Srivastava, Jeremy S Dasen, Hynek Wichterle, Shaun Mahony, Esteban O Mazzoni

Discovery of genes required for body axis and limb formation by global identification of conserved retinoic acid regulated enhancers and silencers
Marie Berenguer, Karolin F. Meyer, Jun Yin, Gregg Duester

Keystone genes of mammalian tooth patterning and quantification of their expression
Outi Hallikas, Rishi Das Roy, Mona M. Christensen, Elodie Renvoisé, Ana-Marija Sulic, Jukka Jernvall

Identification of Biomarkers Driving Blood Cell Development
Maryam Nazarieh, Volkhard Helms

The Human Accelerated Region HACNS1 modifies developmental gene expression in humanized mice
Emily V. Dutrow, Deena Emera, Kristina Yim, Severin Uebbing, Acadia A. Kocher, Martina Krenzer, Timothy Nottoli, Daniel B. Burkhardt, Smita Krishnaswamy, Angeliki Louvi, James P. Noonan

Cell type- and stage-specific expression of Otx2 is coordinated by a cohort of transcription factors and multiple cis-regulatory modules in the retina
Candace Chan, Nicolas Lonfat, Rong Zhao, Alexander Davis, Liang Li, Man-Ru Wu, Cheng-Hui Lin, Zhe Ji, Constance L. Cepko, Sui Wang

Mitf-family transcription factor function is required within cranial neural crest cells to promote choroid fissure closure
Katie L. Sinagoga, Alessandra M. Larimer-Picciani, Stephanie M. George, Samantha A. Spencer, James A. Lister, Jeffrey M. Gross

The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor
K. Petratou, S. A. Spencer, R. N. Kelsh, J. A. Lister

Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals
Nainoa Richardson, Isabelle Gillot, Elodie P. Gregoire, Sameh A. Youssef, Dirk G. de Rooij, Alain de Bruin, Marie-Cécile De Cian, Marie-Christine Chaboissier

Zebrafish dazl regulates cystogenesis upstream of the meiotic transition and germline stem cell specification and independent of meiotic checkpoints
Sylvain Bertho, Mara Clapp, Torsten U. Banisch, Jan Bandemer, Erez Raz, Florence L. Marlow

TWIST1 homodimers and heterodimers orchestrate lineage-specific differentiation
Xiaochen Fan, Ashley J. Waardenberg, Madeleine Demuth, Pierre Osteil, Jane Sun, David A.F. Loebel, Mark Graham, Patrick P.L. Tam, Nicolas Fossat

Deletion of a conserved Gata2 enhancer impairs haemogenic endothelium programming and adult haematopoiesis
Tomasz Dobrzycki, Christopher B. Mahony, Monika Krecsmarik, Cansu Koyunlar, Rossella Rispoli, Joke Peulen-Zink, Kirsten Gussinklo, Bakhta Fedlaoui, Emma de Pater, Roger Patient, Rui Monteiro

New observations on non-coding RNAs involved in the dual translation system in zebrafish development
Timo M. Breit, Johanna F. B. Pagano, Pjotr L. van der Jagt, Ellis Mittring, Wim A. Ensink, Marina van Olst, Selina van Leeuwen, Wim de Leeuw, Ulrike Nehrdich, Herman P. Spaink, Han Rauwerda, Rob J. Dekker

microRNA profiling of mouse cortical progenitors and neurons reveals miR-486-5p as a novel regulator of neurogenesis
Martina Dori, Daniel Cavalli, Mathias Lesche, Simone Massalini, Leila Haj Abdullah Alieh, Beatriz Cardoso de Toledo, Sharof Khudayberdiev, Gerhard Schratt, Andreas Dahl, Federico Calegari

Longitudinal epi-transcriptome profiling reveals the crucial role of m6A in prenatal skeletal muscle development of pigs
Xinxin Zhang, Yilong Yao, Jinghua Han, Yalan Yang, Yun Chen, Zhonglin Tang, Fei Gao

Massively parallel disruption of enhancers active during human corticogenesis
Evan Geller, Jake Gockley, Deena Emera, Severin Uebbing, Justin Cotney, James P. Noonan

Single cell epigenomic atlas of the developing human brain and organoids
Ryan S. Ziffra, Chang N. Kim, Amy Wilfert, Maximilian Haeussler, Alex M. Casella, Pawel F. Przytycki, Anat Kreimer, Katherine S. Pollard, Seth A. Ament, Evan E. Eichler, Nadav Ahituv, Tomasz J. Nowakowski

A single cell transcriptional atlas of early synovial joint development
Qin Bian, Yu-Hao Cheng, Jordan P Wilson, Dong Won Kim, Hong Wang, Seth Blackshaw, Patrick Cahan

Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mouse
K. M. Hurskainen, I. Mižíková, D. P. Cook, C. Cyr-Depauw, F. Lesage, N. Andersson, E. Helle, L. Renesme, R.P. Jankov, M. Heikinheimo, B. C. Vanderhyden, B Thébaud

Chromatin regulatory dynamics of early development and regional specification in a directed differentiation model of the human small intestine
Yu-Han Hung, Sha Huang, Michael K. Dame, Jason R. Spence, Praveen Sethupathy

Polycomb-mediated repression compensates for loss of postnatal DNA methylation in excitatory neurons
Junhao Li, Antonio Pinto-Duarte, Mark Zander, Chi-Yu Lai, Julia Osteen, Linjing Fang, Chongyuan Luo, Jacinta D. Lucero, Rosa Gomez-Castanon, Joseph R. Nery, Isai Silva-Garcia, Yan Pang, Terrence J. Sejnowski, Susan B. Powell, Joseph R. Ecker, Eran A. Mukamel, M. Margarita Behrens

Conserved epigenetic regulatory logic infers genes governing cell identity
Woo Jun Shim, Enakshi Sinniah, Jun Xu, Burcu Vitrinel, Michael Alexanian, Gaia Andreoletti, Sophie Shen, Brad Balderson, Guangdun Peng, Naihe Jing, Yuliangzi Sun, Yash Chhabra, Yuliang Wang, Patrick P L Tam, Aaron Smith, Michael Piper, Lionel Christiaen, Quan Nguyen, Mikael Bodén, Nathan J. Palpant

poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance
Aditi Shukla, Jenny Yan, Daniel J. Pagano, Anne E. Dodson, Yuhan Fei, Josh Gorham, J.G. Seidman, Marvin Wickens, Scott Kennedy

A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord
Aaron M. Allen, Megan C. Neville, Sebastian Birtles, Vincent Croset, Christoph D. Treiber, Scott Waddell, Stephen F. Goodwin

A single-cell survey of Drosophila blood
Sudhir Gopal Tattikota, Yanhui Hu, Yifang Liu, Bumsik Cho, Victor Barrera, Michael Steinbaugh, Sang-Ho Yoon, Aram Comjean, Fangge Li, Franz Dervis, Ruei-Jiun Hung, Jin-Wu Nam, Shannan Ho Sui, Jiwon Shim, Norbert Perrimon

Temporal specificity and heterogeneity of the fly immune cells’ transcriptional landscape
Pierre B. Cattenoz, Rosy Sakr, Alexia Pavlidaki, Claude Delaporte, Andrea Riba, Nacho Molina, Nivedita Hariharan, Tina Mukherjee, Angela Giangrande

sisterless A is required for the activation of Sex lethal in the germline
Raghav Goyal, Ellen Baxter, Mark Van Doren

Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition
Wen Xi Cao, Sarah Kabelitz, Meera Gupta, Eyan Yeung, Sichun Lin, Christiane Rammelt, Christian Ihling, Filip Pekovic, Timothy C. H. Low, Najeeb U. Siddiqui, Matthew H. K. Cheng, Stephane Angers, Craig A. Smibert, Martin Wühr, Elmar Wahle, Howard D. Lipshitz

Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS
Haluk Lacin, W. Ryan Williamson, Gwyneth M. Card, James B. Skeath, James W. Truman

Topology-driven analysis of protein-protein interaction networks detects functional genetic modules regulating reproductive capacity
Tarun Kumar, Leo Blondel, Cassandra G. Extavour

The Drosophila MLR COMPASS-like complex regulates bantam miRNA expression differentially in the context of cell fate
David J. Ford, Claudia B. Zraly, John Hertenstein Perez, Andrew K. Dingwall

| Stem cells, regeneration & disease modelling

The R2TP chaperone assembles cellular machineries in intestinal CBC stem cells and progenitors
Chloé Maurizy, Claire Abeza, Valérie Pinet, Marina Ferrand, Conception Paul, Julie Bremond, Francina Langa, François Gerbe, Philippe Jay, Céline Verheggen, Nicola Tinari, Dominique Helmlinger, Rossano Lattanzio, Edouard Bertrand, Michael Hahne, Bérengère Pradet-Balade

Notch ligand Dll4 impairs cell recruitment into aortic clusters and limits hematopoietic stem cells
Cristina Porcheri, Ohad Golan, Fernando J. Calero-Nieto, Roshana Thambyrajah, Cristina Ruiz-Herguido, Xiaonan Wang, Francesca Catto, Yolanda Guillen, Roshani Sinha, Jessica González, Sarah J. Kinston, Samanta A. Mariani, Antonio Maglitto, Chris Vink, Elaine Dzierzak, Pierre Charbord, Bertie Göttgens, Lluis Espinosa, David Sprinzak, Anna Bigas

The 9aaTAD activation domains in the four Yamanaka Oct4, Sox2, Myc, and Klf4 transcription factors essential during the stem cell development
Martin Piskacek, Kristina Jendruchova, Martina Rezacova, Marek Havelka, Norbert Gasparik, Alena Hofrova, Andrea Knight

TGFβ superfamily signaling regulates the state of human stem cell pluripotency and competency to create telencephalic organoids
Momoko Watanabe, Jillian R. Haney, Neda Vishlaghi, Felix Turcios, Jessie E. Buth, Wen Gu, Amanda J. Collier, Osvaldo A. Miranda, Di Chen, Shan Sabri, Amander T. Clark, Kathrin Plath, Heather R. Christofk, Michael J. Gandal, Bennett G. Novitch

Network analysis of promoter interactions reveals the hierarchical differences in genome organisation between human pluripotent states
Peter Chovanec, Amanda J. Collier, Christel Krueger, Csilla Várnai, Stefan Schoenfelder, Anne Corcoran, Peter J. Rugg-Gunn

A Holistic Analysis of the Intestinal Stem Cell Niche Network
Darrick M. Hansen, Paloma Ivon Meneses Giles, Xi C. He, Shiyuan Chen, Ariel Paulson, Christopher M. Dekaney, Jennifer Wang, Deqing Hu, Aparna Venkatraman, Woosook Kim, John Kaddis, Barbara J. Olack, James C.Y. Dunn, Calvin Kuo, Susan Henning, Alan M. Hanash, Courtney W. Houchen, John Lynch, Martin G. Martin, Joyce C. Niland, Matthias Stelzner, Melissa Wong, Timothy C. Wang, Jian Yu, Kelley Yan, Linheng Li

Functional Mature Human Microglia Developed in Human iPSC Microglial Chimeric Mouse Brain
Ranjie Xu, Andrew J. Boreland, Xiaoxi Li, Anthony Posyton, Kelvin Kwan, Ronald P. Hart, Peng Jiang

Characterization of human-iPSCs derived spinal motor neurons by single-cell RNA sequencing
Louise Thiry, Regan Hamel, Stefano Pluchino, Thomas Durcan, Stefano Stifani

Transcriptome and Proteome analysis of Hemidactylus frenatus during initial stages of tail regeneration
Sai Pawan, Sarena Banu, Mohammed M Idris

Pattern regulation in a regenerating jellyfish
Chiara Sinigaglia, Sophie Peron, Julia Steger, Evelyn Houliston, Lucas Leclère

Active Notch Signaling is Required for Arm Regeneration in a Brittle Star
Vladimir Mashanov, Jennifer Akiona, Maleana Khoury, Jacob Ferrier, Robert Reid, Denis Jacob Machado, Olga Zueva, Daniel Janies

Integrin-alpha-6+ Stem Cells (ISCs) are responsible for whole body regeneration in an invertebrate chordate
Susannah H. Kassmer, Adam Langenbacher, Anthony W. De Tomaso

Glucose metabolism promotes neonatal heart regeneration
Viviana M Fajardo Martinez, Iris Feng, Bao Ying Chen, Cesar A Perez, Baochen Shi, Peter Clark, Rong Tian, Ching-Ling Lien, Matteo Pellegrini, Heather Christofk, Haruko Nakano, Atsushi Nakano

Synergic coordination of stem cells is required to induce a regenerative response in anthozoan cnidarians
Aldine R. Amiel, Kevin Foucher, Solène Ferreira, Eric Röttinger

Citrullination regulates wound responses and tissue regeneration in zebrafish
Netta Golenberg, Jayne M. Squirrell, David A. Bennin, Julie Rindy, Paige E. Pistono, Kevin W. Eliceiri, Miriam A. Shelef, Junsu Kang, Anna Huttenlocher

Skip segment Hirschsprung disease: modelling the trans-mesenteric origin of the enteric nervous system in the human colon
Donald F Newgreen, James M Osborne, Dongcheng Zhang

The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification
Sally H. Cross, Lisa Mckie, Toby W. Hurd, Sam Riley, Jimi Wills, Alun R. Barnard, Fiona Young, Robert E. MacLaren, Ian J. Jackson

A Genetic Screen Links the Disease-Associated Nab2 RNA-Binding Protein to the Planar Cell Polarity Pathway in Drosophila melanogaster
Wei-Hsuan Lee, Edwin Corgiat, J. Christopher Rounds, Zenyth Shepherd, Anita H. Corbett, Kenneth H. Moberg

Loss of the Reissner Fiber and increased URP neuropeptide signaling underlie scoliosis in a zebrafish ciliopathy mutant
Christine Vesque, Isabelle Anselme, Guillaume Pezeron, Yasmine Cantaut-Belarif, Alexis Eschstruth, Morgane Djebar, Diego López Santos, Hélène Le Ribeuz, Arnim Jenett, Hanane Khoury, Joëlle Véziers, Caroline Parmentier, Sylvie Schneider-Maunoury

A translational kidney organoid system bolsters human relevance of clinical development candidate
Amy Duyen Westerling-Bui, Thomas W. Soare, Srininivasan Venkatachalan, Michael DeRan, Eva Maria Fast, Alyssa B. Fanelli, Sergii Kyrychenko, Hien Hoang, Grinal M. Corriea, Wei Zhang, Maolin Yu, Matthew Daniels, Goran Malojcic, Xin-Ru Pan-Zhou, Mark W. Ledeboer, Jean-Christophe Harmange, Maheswarareddy Emani, Thomas T. Tibbitts, John F. Reilly, Peter Mundel

Characterization of human iPSC-derived astrocytes with potential for disease modeling and drug discovery
Vincent Soubannier, Gilles Maussion, Mathilde Chaineau, Veronika Sigutova, Guy Rouleau, Thomas Durcan, Stefano Stifani

mtor Haploinsufficiency Ameliorates Renal Cyst Formation in Adult Zebrafish tmem67 Mutants
Ping Zhu, Qi Qiu, Peter C. Harris, Xiaolei Xu, Xueying Lin

Characterization of SETD1A haploinsufficiency in humans and Drosophila defines a novel neurodevelopmental syndrome
Joost Kummeling, Diante E Stremmelaar, Nicholas Raun, Margot RF Reijnders, Marjolein H Willemsen, Martina Ruiterkamp-Versteeg, Marga Schepens, Calvin CO Man, Christian Gilissen, Megan T Cho, Kirsty McWalter, Margje Sinnema, James W Wheless, Marleen EH Simon, Casie A Genetti, Alicia M Casey, Paulien A Terhal, Jasper J van der Smagt, Koen L van Gassen, Pascal Joset, Angela Bahr, Katharina Steindl, Anita Rauch, Elmar Keller, Annick Raas-Rothschild, David A Koolen, Pankaj B Agrawal, Trevor L Hoffman, Nina N Powell-Hamilton, Isabelle Thiffault, Kendra Engleman, Dihong Zhou, Olaf Bodamer, Julia Hoefele, Korbinian M Riedhammer, Eva MC Schwaibold, Velibor Tasic, Dirk Schubert, Deniz Top, Rolph Pfundt, Martin R Higgs, Jamie M Kramer, Tjitske Kleefstra

Cleft lip and cleft palate (CL/P) in Esrp1 KO mice is associated with alterations in Wnt signaling and epithelial-mesenchymal crosstalk
SungKyoung Lee, Matthew J. Sears, Zijun Zhang, Hong Li, Imad Salhab, Philippe Krebs, Yi Xing, Hyun-Duck Nah, Trevor Williams, Russ P. Carstens

NCBP2 modulates neurodevelopmental defects of the 3q29 deletion in Drosophila and X. laevis models
Mayanglambam Dhruba Singh, Matthew Jensen, Micaela Lasser, Emily Huber, Tanzeen Yusuff, Lucilla Pizzo, Brian Lifschutz, Inshya Desai, Alexis Kubina, Sneha Yennawar, Sydney Kim, Janani Iyer, Diego E. Rincon-Limas, Laura Anne Lowery, Santhosh Girirajan

Transcriptomic changes due to early, chronic alcohol exposure during cortical development implicate regionalization, cell-type specification, synaptogenesis and WNT signaling as primary determinants of fetal alcohol Spectrum Disorders
Máté Fischer, Praveen Chander, Huining Kang, Jason P. Weick

| Plant development

Direct ETTIN-auxin interaction controls chromatin state in gynoecium development
André Kuhn, Sigurd Ramans Harborough, Heather M. McLaughlin, Stefan Kepinski, Lars Østergaard

OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module specifying phloem identity
Eva-Sophie Wallner, Nina Tonn, Friederike Wanke, Vadir Lopéz-Salmerón, Michael Gebert, Christian Wenzl, Jan U. Lohmann, Klaus Harter, Thomas Greb

Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions
Michael J. Prigge, Matthieu Platre, Nikita Kadakia, Yi Zhang, Kathleen Greenham, Whitnie Szutu, Bipin K. Pandey, Rahul Bhosale, Malcolm J. Bennett, Wolfgang Busch, Mark Estelle

Abundant expression of maternal siRNAs is a conserved feature of seed development
Jeffrey W. Grover, Diane Burgess, Timmy Kendall, Abdul Baten, Suresh Pokhrel, Graham J. King, Blake C. Meyers, Michael Freeling, Rebecca A. Mosher

Vein Patterning by Tissue-Specific Auxin Transport
Priyanka Govindaraju, Carla Verna, Tongbo Zhu, Enrico Scarpella

Impact of small RNAs in retrograde signalling pathways in Arabidopsis thaliana
Kristin Habermann, Bhavika Tiwari, Maria Krantz, Stephan O. Adler, Edda Klipp, M. Asif Arif, Wolfgang Frank

Arabidopsis ROOT PHOTOTROPISM2 is a Light-Dependent Dynamic Modulator of Phototropin1
Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito, Tatsuya Sakai

Arabidopsis XTH4 and XTH9 contribute to wood cell expansion and secondary wall formation
Sunita Kushwah, Alicja Banasiak, Nobuyuki Nishikubo, Marta Derba-Maceluch, Mateusz Majda, Satoshi Endo, Vikash Kumar, Leonardo Gomez, Andras Gorzsas, Simon McQueen-Mason, Janet Braam, Björn Sundberg, Ewa J. Mellerowicz

ROOT PATTERNING AND REGENERATION ARE MEDIATED BY THE QUIESCENT CENTER AND INVOLVE BLUEJAY, JACKDAW AND SCARECROW REGULATION OF VASCULATURE FACTORS
Alvaro Sanchez-Corrionero, Pablo Perez-Garcia, Javier Cabrera, Javier Silva-Navas, Juan Perianez-Rodriguez, Inmaculada Gude, Juan Carlos del Pozo, Miguel A. Moreno-Risueno

Boron deficiency-induced root growth inhibition is mediated by brassinosteroid signalling regulation in Arabidopsis
Cheng Zhang, Mingliang He, Sheliang Wang, Liuyang Chu, Chuang Wang, Ningmei Yang, Guangda Ding, Hongmei Cai, Lei Shi, Fangsen Xu

Live-cell imaging of early events following pollen perception in self-incompatible Arabidopsis thaliana
Frédérique Rozier, Lucie Riglet, Chie Kodera, Vincent Bayle, Eléonore Durand, Jonathan Schnabel, Thierry Gaude, Isabelle Fobis-Loisy

MicroRNA function transitions from regulating developmental genes to transposable elements during the maturation of pollen
Cecilia Oliver, Maria Luz Annacondia, Zhenxing Wang, R Keith Slotkin, Claudia Köhler, German Martinez

Cytokinin induction by the jasmonate-induced AP2/ERF115 represses adventitious rooting in Arabidopsis
Abdellah Lakehal, Asma Dob, Zahra Rahneshan, Ondřej Novák, Sacha Escamez, Sanaria Alallaq, Miroslav Strnad, Hannele Tuominen, Catherine Bellini

In vivo mRNA structure regulates miRNA cleavage in Arabidopsis
Minglei Yang, Hugh C. Woolfenden, Yueying Zhang, Xiaofeng Fang, Qi Liu, Maria Louisa Vigh, Jitender Cheema, Xiaofei Yang, Matthew Norris, Sha Yu, Alberto Carbonell, Peter Brodersen, Jiawei Wang, Yiliang Ding

Emergent honeycomb topology of the leaf spongy mesophyll
Aleca M. Borsuk, Adam B. Roddy, Guillaume Théroux-Rancourt, Craig R. Brodersen

Auxin transport network underlies xylem bridge formation between the hemi-parasitic plant Phtheirospermum japonicum and host Arabidopsis
Takanori Wakatake, Satoko Yoshida, Ken Shirasu

Functional role of Polymerase IV during pollen development in Capsella
Zhenxing Wang, Nicolas Butel, Juan Santos-González, Filipe Borges, Jun Yi, Robert A Martienssen, German Martinez, Claudia Köhler

Compensatory guaiacyl lignin biosynthesis at the expense of syringyl lignin in 4CL1-knockout poplar
Chung-Jui Tsai, Peng Xu, Liang-Jiao Xue, Hao Hu, Batbayar Nyamdari, Radnaa Naran, Xiaohong Zhou, Geert Goeminne, Ruili Gao, Erica Gjersing, Joseph Dahlen, Sivakumar Pattathil, Michael G. Hahn, Mark F. Davis, John Ralph, Wout Boerjan, Scott A. Harding

The regulatory landscape of early maize inflorescence development
Rajiv K. Parvathaneni, Edoardo Bertolini, Md Shamimuzzaman, Daniel Vera, Pei-Yau Lung, Brian R. Rice, Patrick J. Brown, Alexander E. Lipka, Hank W. Bass, Andrea L. Eveland

LYS3 encodes a prolamin-box-binding transcription factor that controls embryo growth in barley and wheat
Beata Orman-Ligeza, Philippa Borrill, Tansy Chia, Marcella Chirico, Jaroslav Doležel, Sinead Drea, Miroslava Karafiátová, Nicole Schatlowski, Charles U. Solomon, Burkhard Steuernagel, Brande B. H. Wulff, Cristobal Uauy, Kay Trafford

Gene expression data support the hypothesis that Isoetes rootlets are true roots and not modified leaves
Alexander J. Hetherington, David M. Emms, Steven Kelly, Liam Dolan

Gene regulatory networks associated with lateral root and nodule development in soybean
Shuchi Smita, Jason Kiehne, Sajag Adhikari, Erliang Zeng, Qin Ma, Senthil Subramanian

Evo-devo & evo

Cell shape changes during larval body plan development in Clytia hemisphaerica
Yulia Kraus, Sandra Chevalier, Evelyn Houliston

Evidence of multifaceted functions of codon usage in translation within the model beetle Tribolium castaneum
Carrie A. Whittle, Arpita Kulkarni, Cassandra G. Extavour

Genomic adaptations to aquatic and aerial life in mayflies and the origin of wings in insects
Isabel Almudi, Joel Vizueta, Alex de Mendoza, Chris Wyatt, Ferdinand Marletaz, Panos Firbas, Roberto Feuda, Giulio Masiero, Patricia Medina, Ana Alcaina, Fernando Cruz, Jessica Gómez-Garrido, Marta Gut, Tyler S. Alioto, Carlos Vargas-Chavez, Kristofer Davie, Bernhard Misof, Josefa González, Stein Aerts, Ryan Lister, Jordi Paps, Julio Rozas, Alejandro Sánchez-Gracia, Manuel Irimia, Ignacio Maeso, Fernando Casares

Co-evolving wing spots and mating displays are genetically separable traits in Drosophila
Jonathan H. Massey, Gavin R. Rice, Anggun Firdaus, Chi-Yang Chen, Shu-Dan Yeh, David L. Stern, Patricia J. Wittkopp

Dscam homophilic specificity is generated by high order cis-multimers coupled with trans self-binding of variable Ig1 in Chelicerata
Fengyan Zhou, Guozheng Cao, Songjun Dai, Guo Li, Hao Li, Zhu Ding, Shouqing Hou, Bingbing Xu, Wendong You, Feng Shi, Xiaofeng Yang, Yongfeng Jin

H3K27me3 natural variation selectively marks genes predicted to be important for differentiation in unicellular algae
Xue Zhao, Achal Rastogi, Anne Flore Deton Cabanillas, Ouardia Ait Mohamed, Catherine Cantrel, Berangère Lombard, Omer Murik, Auguste Genovesio, Chris Bowler, Daniel Bouyer, Damarys Loew, Xin Lin, Alaguraj Veluchamy, Fabio Rocha Jimenez Vieira, Leila Tirichine

Two distinct bacterial biofilm components trigger metamorphosis in the colonial hydrozoan Hydractinia echinata
Huijuan Guo, Maja Rischer, Martin Westermann, Christine Beemelmanns

Cell biology

The nucleus acts as a ruler tailoring cell responses to spatial constraints
A.J. Lomakin, C.J. Cattin, D. Cuvelier, Z. Alraies, M. Molina, G. Nader, N. Srivastava, J.M. Garcia-Arcos, I.Y. Zhitnyak, A. Bhargava, M.K. Driscoll, E.S. Welf, R. Fiolka, R.J. Petrie, N. Manel, A.M. Lennon-Duménil, D.J. Müller, M. Piel

Emergence of single cell mechanical behavior and polarity within epithelial monolayers drives collective cell migration
Shreyansh Jain, Victoire M.L. Cachoux, Gautham H.N.S. Narayana, Simon de Beco, Joseph D’Alessandro, Victor Cellerin, Tianchi Chen, Mélina L. Heuzé, Philippe Marcq, René-Marc Mège, Alexandre J. Kabla, Chwee Teck Lim, Benoit Ladoux

ERK-mediated mechanochemical waves direct collective cell polarization
Naoya Hino, Leone Rossetti, Ariadna Marin-Llaurado, Kazuhiro Aoki, Xavier Trepat, Michiyuki Matsuda, Tsuyoshi Hirashima

Excess Centrosomes Disrupt Vascular Lumenization and Endothelial Cell Adherens Junctions
Danielle M Berlin, Erich J Kushner, Katy L Davis, Victoria L Bautch

Tyrosine-based Signals Regulate the assembly of Daple•PARD3 Complex at Cell-cell Junctions during Polarized Planar Cell Migration
Jason Ear, Anokhi Saklecha, Navin Rajapakse, Julie Choi, Majid Ghassemian, Irina Kufareva, Pradipta Ghosh

Tuba activates Cdc42 during neuronal polarization downstream of the small GTPase Rab8a
Pamela J. Urrutia, Felipe Bodaleo, Daniel A. Bórquez, Victoria Rozes-Salvador, Cristopher Villablanca, Cecilia Conde, Mitsunori Fukuda, Christian González-Billault

Zygote morphogenesis but not the establishment of cell polarity in Plasmodium berghei is controlled by the small GTPase, RAB11A
H. Patil, K.R. Hughes, L. Lemgruber, N. Philip, N. Dickens, A. P. Waters

Alpha synuclein aggresomes inhibit ciliogenesis and multiple functions of the centrosome
Anila Iqbal, Marta Baldrighi, Jennifer N. Murdoch, Angeleen Fleming, Christopher J. Wilkinson

Ubiquitin Links Smoothened to Intraflagellar Transport to Regulate Hedgehog Signaling
Paurav B. Desai, Michael W. Stuck, Bo Lv, Gregory J. Pazour

Aurora B and C kinases regulate prophase exit and chromosome segregation during spermatogenesis
Stephen R. Wellard, Karen Schindler, Philip Jordan

Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation
Clare Rogerson, Duncan Wotherspoon, Ryan F L O’Shaughnessy

Modelling

Perturbation analysis of a multi-morphogen Turing Reaction-Diffusion stripe patterning system reveals key regulatory interactions
Andrew D. Economou, Nicholas A.M. Monk, Jeremy B.A. Green

A mathematical model for cell polarization in zebrafish primordial germ cells
Carolin Dirks, Paul Striewski, Benedikt Wirth, Anne Aalto, Adan Olguin-Olguin, Erez Raz

Large-Scale Survey of Cell-Differentiation Programs in a Generative Model Reveals Regeneration as an Epiphenomenon of Development
Somya Mani, Tsvi Tlusty

Homeorhesis in Waddington’s Landscape by Epigenetic Feedback Regulation
Yuuki Matsushita, Kunihiko Kaneko

Multiscale mechanical model for cell division orientation in developing biological systems
B. Leggio, J. Laussu, E. Faure, P. Lemaire, C. Godin

Collective cell migration and residual stress accumulation: modeling consideration
I. Pajic-Lijakovic, M. Milivojevic

Mechanical cell competition in heterogeneous epithelial tissues
R. J. Murphy, P. R. Buenzli, R. E. Baker, M. J. Simpson

A Stochastic Model for Actin Waves in Eukaryotic Cells
Jifeng Hu, Varunyu Khamviwath, Hans G. Othmer

Tools & resources

Visualizing the metazoan proliferation-differentiation decision in vivo
Abraham Q. Kohrman, Rebecca C. Adikes, Jayson J. Smith, Michael A. Q. Martinez, Taylor N. Medwig-Kinney, Nicholas J. Palmisano, Maria D. Sallee, Ononnah B. Ahmed, Nicholas Weeks, Nuri Kim, Simeiyun Liu, Wan Zhang, Ariel M. Pani, David Q. Matus

Computationally Enhanced Quantitative Phase Microscopy Reveals Autonomous Oscillations in Mammalian Cell Growth
Xili Liu, Seungeun Oh, Leonid Peshkin, Marc W. Kirschner

Multi-photon attenuation-compensated light-sheet fluorescence microscopy
Madhu Veettikazhy, Jonathan Nylk, Federico Gasparoli, Adrià Escobet-Montalbán, Anders Kragh Hansen, Dominik Marti, Peter Eskil Andersen, Kishan Dholakia

Optimization and functionalization of red-shifted rhodamine dyes
Jonathan B. Grimm, Ariana N. Tkachuk, Heejun Choi, Boaz Mohar, Natalie Falco, Ronak Patel, Jennifer Lippincott-Schwartz, Timothy A. Brown, Luke D. Lavis

SMALL ALPHAHERPESVIRUS LATENCY-ASSOCIATED PROMOTERS DRIVE EFFICIENT AND LONG-TERM TRANSGENE EXPRESSION IN THE CENTRAL NERVOUS SYSTEM
Carola J. Maturana, Jessica L. Verpeut, Thomas J. Pisano, Zahra M. Dhanerawala, Andrew Esteves, Lynn W. Enquist, Esteban A. Engel

Rapid and Inexpensive Preparation of Genome-Wide Nucleosome Footprints from Model and Non-Model Organisms
Laura E McKnight, Johnathan G Crandall, Thomas B Bailey, Orion GB Banks, Kona N Orlandi, Vi N Truong, Grace L Waddell, Elizabeth T Wiles, Drake A Donovan, Scott D Hansen, Eric U Selker, Jeffrey N McKnight

A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila
Fillip Port, Claudia Strein, Mona Stricker, Benedikt Rauscher, Florian Heigwer, Jun Zhou, Celine Beyersdörffer, Jana Frei, Amy Hess, Katharina Kern, Roberta Malamud, Bojana Pavlovic, Kristin Rädecke, Lukas Schmitt, Lukas Voos, Erica Valentini, Michael Boutros

Principles for rational Cas13d guide design
Hans-Hermann Wessels, Alejandro Méndez-Mancilla, Xinyi Guo, Mateusz Legut, Zharko Daniloski, Neville E. Sanjana

Non-destructive enzymatic deamination enables single molecule long read sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single base resolution
Zhiyi Sun, Romualdas Vaisvila, Bo Yan, Chloe Baum, Lana Saleh, Mala Samaranayake, Shengxi Guan, Nan Dai, Ivan R. Corrêa Jr., Sriharsa Pradhan, Theodore B. Davis, Thomas C. Evans Jr., Laurence M. Ettwiller

Extensive Mammalian Germline Genome Engineering
Yanan Yue, Yinan Kan, Weihong Xu, Hong-Ye Zhao, Yixuan Zhou, Xiaobin Song, Jiajia Wu, Juan Xiong, Dharmendra Goswami, Meng Yang, Lydia Lamriben, Mengyuan Xu, Qi Zhang, Yu Luo, Jianxiong Guo, Shengyi Mao, Deling Jiao, Tien Dat Nguyen, Zhuo Li, Jacob V. Layer, Malin Li, Violette Paragas, Michele E. Youd, Zhongquan Sun, Yuan Ding, Weilin Wang, Hongwei Dou, Lingling Song, Xueqiong Wang, Lei Le, Xin Fang, Haydy George, Ranjith Anand, Shi Yun Wang, William F. Westlin, Marc Güell, James Markmann, Wenning Qin, Yangbin Gao, Hong-jiang Wei, George M. Church, Luhan Yang

Research practice & education

Grant reviewer perceptions of the quality and effectiveness of panel discussion
Stephen A. Gallo, Karen B. Schmaling, Lisa A. Thompson, Scott R. Glisson

Science communication in online media: influence of press releases on coverage of genetics and CRISPR
Rafał Grochala

An illustration of reproducibility in neuroscience research in the absence of selective reporting
Xiang-Zhen Kong, ENIGMA Laterality Working Group, Clyde Francks

Women and Men Were Proportionally Represented Among Speakers at Major National Neurology Conferences in 2017
Mollie McDermott, James F. Burke, Haley McCalpin, Anita V. Shelgikar, Douglas J. Gelb, Abbey Dunn, Nicholas J. Beimer, Zachary N. London

Implicit bias is strongest when assessing top candidates
Emma R Andersson, Carolina Hagberg, Sara Hägg

Is the replication crisis a problem for biologists? A geometric morphometric approach
Juan Vrdoljak, Kevin Imanol Sanchez, Roberto Arreola-Ramos, Emilce Guadalupe Diaz Huesa, Alejandro Villagra, Luciano Javier Avila, Mariana Morando

Insights from a survey-based analysis of the academic job market
Jason D. Fernandes, Sarvenaz Sarabipour, Christopher T. Smith, Natalie M. Niemi, Nafisa M. Jadavji, Ariangela J. Kozik, Alex S. Holehouse, Vikas Pejaver, Orsolya Symmons, Alexandre W. Bisson Filho, Amanda Haage

Efficient data management infrastructure for the integration of imaging and omics data in life science research
Luis Kuhn Cuellar, Andreas Friedrich, Gisela Gabernet, Luis de la Garza, Sven Fillinger, Adrian Seyboldt, Sven zur Oven-Krockhaus, Friederike Wanke, Sandra Richter, Wolfgang M. Thaiss, Marius Horger, Nisar Malek, Klaus Harter, Michael Bitzer, Sven Nahnsen

Tracking self-citations in academic publishing
Ameni Kacem, Justin W. Flatt, Philipp Mayr

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The closing date for applications is 15 February 2020

The Mass lab is looking for a postdoctoral candidate with a strong neuroscience, neurodevelopment and/or immunology background to study the role of nanoplastics in the development of neurological disorders as part of an ERC starting grant project. The position is for 2 years with a possible extension. The employment is planned to start on 1 April or upon agreement.

The goal of the NanoGlia project is to determine which type of plastic particles can pass from the mother to the developing fetus, whether this can lead to neurodevelopmental disorders and whether this is a microglia-dependent process. To achieve this, a guinea pig model is used due to its anatomical similarity to the human placenta. Further methods include histology, electrophysiology, single-cell RNA-sequencing, and flow cytometry.

About us

Our lab is located in the LIMES Institute, which is highly interdisciplinary, addresses basic research questions in developmental biology, immunology, genetics, and biochemistry and thus provides all the basic equipment required for the project. For more specific methodology we are closely collaborating with groups at the DZNE, Life&Brain and the Medical Faculty.

Qualifications

• The candidate is required to hold a PhD degree in neuroscience/developmental biology or similar topics
• Postdoctoral experience in the same areas is an advantage
• We are looking for candidates with hands-on experience or an interest in expanding their knowledge in molecular biology, electrophysiology, high-dimensional flow cytometry, confocal microscopy, and bioinformatic analyses (particularly scRNA-seq)
• Experience with guinea pigs as a model and background in R/Python is a plus
• Finally, we are looking for applicants with a good track record of peer-reviewed scientific publications and teamwork

We offer

• A thriving academic environment
• A professional career development program
• Participation in the university-wide pension system (VBL)
• Access to extensive university sports program
• A salary based on the TV-L scale (E13, 100%)

The university is committed to diversity and equal opportunity. It is certified as a family-friendly university. Applications from suitable candidates with a certified disability or equivalent status are particularly welcome.

For further information, please contact Professor Elvira Mass by email:

elvira.mass@uni-bonn.de

Please send your complete application as 1 combined PDF file including cover letter, CV, list of references (full address, incl. email and phone number), and list of publications to

elvira.mass@uni-bonn.de

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In this episode of Genetics Unzipped Kat Arney explores the myths and misconceptions behind two of the most iconic images in evolutionary biology: the much-parodied March of Progress – a series of human ancestors walking across the page, portraying the inexorable journey from monkey to man – and the famous finches of the Galapagos islands, which are supposedly the inspiration for Charles Darwin’s theory of natural selection.

Where did these infamous images come from, and do they really show what everyone seems to think they do?

Listen now through the player below, or subscribe from Apple podcasts/iTunes, Spotify and all good podcast apps to make sure you get the latest episodes and catch up on our back catalogue.

Full transcript and show notes available from GeneticsUnzipped.com

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