Applications are invited from highly motivated individuals interested in fundamental mechanisms of neurodevelopment and disease.The focus of the project is to understand neural developmental and behavioural phenotypes in mouse models of Tuberous Sclerosis Complex (TSC1 conditional mouse mutant). The fellowship is in the laboratories of Sara Wilson at the Department of Integrative Medical Biology (IMB), and Leif Carlsson at UCMM both laboratories at Umeå University, Sweden. The facilities provide an interactive modern environment with easy access to good core facilities. The fellowship is administratively placed at IMB, which is an interdisciplinary department focusing on questions in basic and medical sciences. The fellowship is funded for two years and is available immediately. The working ‘day to day’ language in the laboratories is English.
Background of the candidate:
Scientists with a keen interest in developmental neuroscience are encouraged to apply!
Technical experience with mouse genetics and handling, developmental biology, neuroscience, molecular and/or cell biology. Experience with rodent behaviour analysis is an advantage but not required.Technical experience with imaging, molecular biology, immunohistochemistry, in situ hybridisation, vertebrate embryonic model systems is advantageous. Technical experience with embryo electroporation and /or neurite outgrowth/migration assays will also be positively evaluated. Full training will be given!
Qualifications of the candidate:
The successful candidate will have or about to receive a Ph.D. in a relevant discipline, have good communication skills and be proficient in written and spoken English. The most successful scientist will have a high level of motivation, be organised rigorous and have the ability to work both independently and within a team.
Application:
Please submit your application (reference 2019SW7) by 20thAugust 2019 to sara.wilson@umu.se by sending the following documents as a single pdf file:
A short cover letter (not more than 1 page) to include a description of your research experience and suitability for the position.
Curriculum Vitae including: publication list, technical expertise, names and contact information for three referees.
Welcome to our monthly trawl for developmental biology (and related) preprints.
Another big haul this month covering everything from great ape cerebral organoids to collectively contracting choanoflagellates, the genes that control iris development to the signals and forces making boundaries in the hindbrain. The preprints were hosted on bioRxiv, PeerJ, andarXiv. Let us know if we missed anything, and use these links to get to the section you want:
Genomic architecture of Shh dependent cochlear morphogenesis
Victor Muthu, Alex. M. Rohacek, Yao Yao, Staci M. Rakowiecki, Alexander S. Brown, Ying-Tao Zhao, James Meyers, Kyoung-Jae Won, Shweta Ramdas, Christopher D. Brown, Kevin A. Peterson, Douglas J. Epstein
Kidney organoids from Hildebrandt, et al.’s preprint
Control iPSC lines with clinically annotated genetic variants for versatile multi-lineage differentiation
Matthew R Hildebrandt, Miriam S Reuter, Wei Wei, Naeimeh Tayebi, Jiajie Liu, Sazia Sharmin, Jaap Mulder, L Stephen Lesperance, Patrick M Brauer, Caroline Kinnear, Alina Piekna, Asli Romm, Jennifer Howe, Peter Pasceri, Rebecca S Mok, Guoliang Meng, Matthew Rozycki, Deivid de Carvalho Rodrigues, Elisa C Martinez, Michael J Szego, Juan Carlos Zúñiga-Pflücker, Michele K Anderson, Steven A Prescott, Norman D Rosenblum, Binita M Kamath, Seema Mital, Stephen W Scherer, James Ellis
Early Stem Cell Aging in the Mature Brain
Albina Ibrayeva, Maxwell Bay, Elbert Pu, David Jörg, Lei Peng, Heechul Jun, Naibo Zhang, Daniel Aaron, Congrui Lin, Galen Resler, Mi-Hyeon Jang, Benjamin D. Simons, Michael A. Bonaguidi
Zebrafish Dscaml1 is Essential for Retinal Patterning and Function of Oculomotor Subcircuits
Manxiu Ma, Alexandro D. Ramirez, Tong Wang, Rachel L. Roberts, Katherine E. Harmon, David Schoppik, Avirale Sharma, Christopher Kuang, Stephanie L. Goei, James A. Gagnon, Steve Zimmerman, Shengdar Q. Tsai, Deepak Reyon, J. Keith Joung, Emre R. F. Aksay, Alexander F. Schier, Y. Albert Pan
Multiparametric phenotyping of compound effects on patient derived organoids
Johannes Betge, Niklas Rindtorff, Jan Sauer, Benedikt Rauscher, Clara Dingert, Haristi Gaitantzi, Frank Herweck, Thilo Miersch, Erica Valentini, Veronika Hauber, Tobias Gutting, Larissa Frank, Sebastian Belle, Timo Gaiser, Inga Buchholz, Ralf Jesenofsky, Nicolai Härtel, Tianzuo Zhan, Bernd Fischer, Katja Breitkopf-Heinlein, Elke Burgermeister, Matthias P. Ebert, Michael Boutros
Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture
Hui-Hsuan Kuo, Xiaozhi Gao, Jean-Marc DeKeyser, K. Ashley Fetterman, Emily A. Pinheiro, Carly J. Weddle, Michael V. Orman, Marisol Romero-Tejeda, Mariam Jouni, Malorie Blancard, Tarek Magdy, Conrad Epting, Alfred L. George Jr., Paul W. Burridge
CLIJ: Enabling GPU-accelerated image processing in Fiji
Robert Haase, Loic A. Royer, Peter Steinbach, Deborah Schmidt, Alexandr Dibrov, Uwe Schmidt, Martin Weigert, Nicola Maghelli, Pavel Tomancak, Florian Jug, Eugene W. Myers
Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells
Wesley A. Wierson, Brandon W. Simone, Zachary WareJoncas, Carla Mann, Jordan M. Welker, Bibekananda Kar, William A. C. Gendron, Michael A. Barry, Karl J. Clark, Drena L. Dobbs, Maura A. McGrail, Stephen C. Ekker, Jeffrey J. Essner
Cross-species blastocyst chimerism between nonhuman primates using iPSCs
Morteza Roodgar, Fabian P. Suchy, Vivek Bajpai, Jose G. Viches-Moure, Joydeep Bhadury, Angelos Oikonomopoulos, Joseph C. Wu, Joseph L. Mankowski, Kyle M. Loh, Hiromitsu Nakauchi, Catherine A. VandeVoort, Michael P. Snyder
Purified Aequorea proteins from Lambert, et al.’s preprint
Aequorea victoria’s secrets
Gerard G. Lambert, Hadrien Depernet, Guillaume Gotthard, Darrin T. Schultz, Isabelle Navizet, Talley Lambert, Daphne S. Bindels, Vincent Levesque, Jennifer N. Moffatt, Anya Salih, Antoine Royant, Nathan C. Shaner
You can also read the Research Highlight for this press released article.
The capacity of the human central nervous system to regenerate after injury or illness is limited, and the resulting functional impairments carry a vast societal and personal burden. In glaucoma, degeneration of retinal ganglion cells (RGCs) – the axons of which form the optic nerve connecting the retina to the brain – leads to permanent blindness; there is currently no effective treatment for RGC degeneration. Now, University of Nebraska Medical Center researcher Iqbal Ahmad and colleagues show that human RGCs can be regenerated in an in vitro setting helped by lessons learned in rodent models. The discovery is detailed in the journal Development.
“This finding could lead to new methods of screening for drugs and genes impacted by glaucoma to help treat and possibly reverse vision loss in people suffering from the disease,” said Dr. Ahmad, a professor in the department of ophthalmology and visual sciences at UNMC.
RGCs are key in sending messages to the brain through a series of synapses and connections that tell us what the eye sees. In people who suffer from glaucoma, it’s the degeneration of these cells that lead to loss of sight, Dr. Ahmad said.
Dr. Ahmad and his team of investigators found that when the mTOR signaling pathway, present in all cell types and essential for cell survival, is activated in RGCs the cells begin to regenerate and thrive. The researchers used a microfluidic chamber system to see how axons regenerated after axotomy.
Dr. Ahmad has spent 25 years studying the stem cell approach to understand and treat glaucoma, which is called a silent robber of vision because it strikes without warning or any noticeable symptoms. Glaucoma is the second leading cause of irreversible blindness and affects more than 3 million people in the United States and 60 million people worldwide.
The significance of this work, Dr. Ahmad said, is that it is done using human adult pluripotent stem cells, whereas previous work was done only in rats and mice. While those animal models provided insight into better understanding the disease progression of glaucoma, research using human RGCs will translate more readily when it comes to potential drug and gene therapies, he said. His lab has already applied for a patent on the technology that shows how RGCs can be regenerated.
“We are hopeful this process will bring us one step closer to recapturing sight in those patients who suffer from vision loss because of glaucoma,” he said.
This new version of the Quintay2020 course is organized with the collaboration of the Marine Biology Laboratory (MBL, Woods Hole, USA). We invite students to submit their application before the deadline (31st July 2019). At the end of the Quintay2020 course, the two best students will receive a prize, which consists in a place to attend the MBL Embryology course (Woods Hole, USA) during 2020 or 2021 and a full fellowship to attend the MBL course.
Quintay2020 is a practical and lecture course by the best Developmental Biologist such as Nipam Patel (MBL, U Chicago, USA), David McClay (Duke University, USA), Alejandro Sanchez-Alvarado (HHMI, Stower Institute for Medical Research, USA), Claudio Stern (University College London, UK), Andrea Streit (King’s College London, UK), Angela Nieto (Instituto de Neurociencia, Alicante, Spain), Sally Moody (George Washington University, USA), Cecilia Moens (Fred Hutchinson Cancer Research Center, USA), Alfonso Martinez-Arias (University of Cambridge, UK), John Ewer (U. Valparaiso, Chile) and Roberto Mayor (University College London, UK).
The course is intended for Latin American and no-Latin American applicants, as we believe that the interaction between the students will establish links and promote a culture of international collaboration that will further contribute to the field. Fellowships will be available
The course will take place in the summer of the south hemisphere (6th to 18th January 2020), in the beautiful fishing village of Quintay, at the Centre for Marine Biological Research (CIMARQ, in Spanish).
EchinoBase is hiring a Bioinformatician to work in the lab of Veronica Hinman in the Department of Biological Sciences at Carnegie Mellon University (Pittsburgh PA). EchinoBaseis an online platform that houses genomic and transcriptomic resources of echinoderms. The mission of EchinoBase is to serve as a central database for an international community of researchers interested in understanding echinoderm biology from a genomic perspective. In this aim, EchinoBase maintains information on the organization, function and evolution of echinoderm genes. In this role, you will work alongside other EchinoBase personnel.
The Department of Biological Sciences at CMU is the physical home of EchinoBase. Two active research labs in the department use echinoderms as model systems. This provides an excellent opportunity to interface firsthand with members of the community. As an NIH-funded community resource, EchinoBase provides an encouraging environment with many training opportunities available.
Core responsibilities include:
Assembling and annotating echinoderm genomes
Establishing and maintaining high-quality pipelines for analyzing genomic and transcriptomic datasets
Developing web-based data viewers
Generating and contributing content to EchinoBase, including FAQ pages, tutorials and publications
Qualifications:
MSc. or a Ph.D. is desirable in life sciences or related field
Experience with bioinformatic data analysis
Familiarity with genomics and molecular biology
Ambitious, problem solver with the ability to prioritize work
Outstanding attention to detail and strong analytical skills
Excellent verbal and written communication skills
More details and application instructions can be found here.
EchinoBase is hiring a Biocurator to work within the laboratory of Charles Ettensohn in the Department of Biological Sciences at Carnegie Mellon University. EchinoBase is an online platform that houses genomic and transcriptomic resources of echinoderms. The mission of EchinoBase is to serve as a central database for an international community of researchers interested in understanding echinoderm biology from a genomic perspective. In this aim, EchinoBase maintains information on the organization, function and evolution of echinoderm genes. Two active research labs in the department use echinoderms as model systems. This provides an excellent opportunity to interface first-hand with members of the community. As an NIH-funded community resource, EchinoBase provides a supportive environment with many training opportunities available.
Core responsibilities include:
Curate existing and new literature on echinoderm biology and highlight this literature on Echinobase.
Evaluate primary literature for data on spatiotemporal gene expression patterns in echinoderms.
Annotate gene expression patterns using standardized anatomical and genetic vocabularies
Integrate published information on echinoderm genes into the existing database.
Interface with existing Echinobase database structure and software.
Communicate with journals and publishers for copyright permissions.
Build trusted relationships with an international community of echinoderm researchers, including providing feedback to researchers.
Generate and contribute content to Echinobase, including FAQ pages, tutorials and publications.
Qualifications:
Knowledge of echinoderm biology and development. A Bachelor’s degree in life sciences is required, although a MSc or PhD is preferred.
Excellent verbal and written communication skills.
Familiarity with literature databases such as Pubmed and Google Scholar.
Ability to read and analyze scientific literature, including experimental details and data limitations.
Self-motivated, detail-oriented problem solver with the ability to prioritize work.
Computer skills, including word processing and spreadsheet applications.
More details and application instructions can be found here.
The Johnston Lab at Columbia University in New York is looking for curious, motivated individuals to join us in research to characterize the molecular targets and pathways that provide plasticity to growing tissues, allowing cells to sense and adapt to changes in their immediate environment. We investigate the role of competitive cell-cell interactions in organ size control and bilateral symmetry during development, and as a model for early phases of cancer. Projects are available to identify biosensors and cellular mediators of tissue fitness during animal growth. Our work combines classical genetics, functional transcriptomics and genomics and biochemical analysis of cell biological processes.
Columbia University Medical Center is renowned for its dedication to high-class biomedical research, with state-of-the-art facilities for functional genomics, proteomics and metabolomics, and offers a friendly, international and exciting scientific environment in New York City.
Candidates should ideally have experience in one or more of the following disciplines: genetics, developmental cell biology, transcriptomics, functional genomics, protein biochemistry. Previous experience with Drosophila would provide an advantage.
For more information about possible research projects and to apply, please contact:
Are you interested in understanding how cooperative behaviour develops a cellular society?
Are you interested in live imaging at single-cell resolution?
Then, we have a fully funded PhD position for you!
Zebrafish Thyroid Gland
The group of Dr. Sumeet Pal Singh at the Institut de Recherche Interdisciplinaire en Biologie Humaine et moléculaire (IRIBHM), an Institute of the Université libre de Bruxelles (ULB), is offering a PhD position starting 01 November, 2019.
The major aim of the group’s research is to understand the development of cellular communities using endocrine organs, specifically thyroid gland and pancreatic beta-cells, as a model system. The research is focussed on using zebrafish as a model system. The junior group uses a broad range of molecular, genetic, next-generation transcriptomic and imaging techniques to investigate developmental processes (https://sumeetpalsingh.github.io/).
The PhD student will be involved in live-imaging intercellular signalling pathways in the thyroid gland (https://sumeetpalsingh.github.io/project/live-imaging), and for developing tools modulating cAMP signalling in thyroid follicular cells. The tool development would additional lead to new models of hypo- and hyperthyroid disease models.
The PhD student is expected to:
Read literature and keep up-to-date with the current knowledge in the field.
Gain independence in planning, conduction and analysis of experiments.
Generate publication quality figures for presentations, manuscripts and grant applications.
Draft manuscripts for publication.
She/he will be required to attend institute seminars, encouraged to participate in after-hours social events and take part in retreats.
She/he will be encouraged to undertake skill-development courses (particularly bioinformatics skills) in the university or online (such as Coursera)
Requirements:
Diplomas and degrees equivalent to a European Union Master’s degree, which includes project work summarized in a written “small thesis”
Three referees willing to provide letter of recommendations
Excellent knowledge of English
Excellent interpersonal and organisational skills
Kindly submit your CV with the contact information for three referees and a letter of motivation by email to sumeetpalsingh@gmail.com.
Excited about discovering regulators of a differentiation program?
Then, this could be an exciting post-doc opportunity for you while ‘Investigating the cell fate decisions during differentiation of ES-cells to thyroid gland using single-cell technologies’.
At the Institut de Recherche Interdisciplinaire en Biologie Humaine et moléculaire (IRIBHM), an Institute of the Université libre de Bruxelles (ULB), the research group of Dr Sabine Costagliola and Dr Sumeet Pal Singhare jointly offering support for Marie Curie Post-Doc application on using single-cell technologies to understand the directed differentiation of ES-cells into thyroid organoid.
The lab of Dr. Costagliola was the first to generate functional thyroid gland from mouse ES cells (Antonica et al., Nature, 2012). Together with Dr. Singh, an expert in single-cell transcriptome analysis (Singh et al., Scientific Reports, 2018), the project will aim to define the trajectory from naïve ES cells to functional thyroid gland using single-cell genomics. We hope to understand the bottlenecks and hurdles in the differentiation process so as to overcome them and generate a more efficient differentiation process.
The call for Marie Curie Post-Doc Fellowships opened on 15 June, 2019 and ends on 01 October, 2019. The Fellowship requires:
Applicants of any age and of any nationality are eligible.
Eligible applicants are Experienced Researchers, i.e.
Holders of a PhD degree obtained max. 7 years before the call deadline.
Applicants without a PhD, provided they have documented full-time equivalent research experience of minimum 4 and maximum 11 years.
An eligibility extension of 15 weeks per child is granted in case of maternity
Eligible applicants must not have resided or carried out their main activity (work, studies, etc.) in Belgium for more than 12 months during the 3 years preceding the call deadline.
This annual 1-day symposium brings together cell biologists from the North of England.
There is a full day of talks and a poster session where junior scientists are encouraged to present their work. PhD students and post-docs are invited to apply for oral and/or poster presentations; prizes will be awarded on the day.
Date: Thursday, 12 Sept 2019
Location:
The University of Manchester
Michael Smith Building
Dover Street
Manchester M13 9PT
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This new version of the Quintay2020 course is organized with the collaboration of the Marine Biology Laboratory (MBL, Woods Hole, USA). We invite students to submit their application before the deadline (31st July 2019). At the end of the Quintay2020 course, the two best students will receive a prize, which consists in a place to attend the MBL Embryology course (Woods Hole, USA) during 2020 or 2021 and a full fellowship to attend the MBL course.
