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SciArt profile: Menelia Vasilopoulou Kampitsi

Posted by , on 16 October 2024

In this SciArt profile, we meet Menelia Vasilopoulou Kampitsi, who studied functional morphology during her PhD and is now working part-time as a scientific illustrator. Menelia takes inspiration from impressionism, the Bauhaus movement, and surrealism, and employs a variety of techniques to create her artwork.

“Femoral pores on the inner thighs of a male lacertid lizard”.  Published in: Baeckens S. (2019). Evolution of animal chemical communication: Insights from non-model species and phylogenetic comparative methods. Belgian Journal of Zoology. 149, 63–93.

Can you tell us about your background and what you work on now?

My academic background began in the fields of organismal biology and ecology, which I studied between Greece and France. I later pursued a doctorate in functional morphology at the FunMorph Lab of the Univeristy of Antwerp in Belgium. It was during my PhD that I recognized the importance of illustration as a tool for communicating complex scientific concepts, both to the academic community and the general public. Toward the end of my doctoral studies, I had the opportunity to take a scientific illustration course in Spain—my first formal step into the world of scientific illustration. Since transitioning to freelance work in 2020, I’ve had the pleasure of collaborating on a variety of projects with PhD students, veterinarians, and researchers.

Over the past few months, I’ve had the chance of collaborating with a team of veterinarians, contributing illustrations to a new book on the psychiatric aspects of cats. This project, so far, has been a learning experience, combining my love for art with a deeper understanding of the behavioral complexities of cats. I will still be working for this project until the end of the year. This opportunity follows a previous collaboration with the same team, where I illustrated a book on canine psychiatry. The original French edition of the first book was published in 2023 by NoLedge Editions, with the English version being released by Springer Nature in 2024.

Aside from this project, I accept commissions from other clients on a wide range of topics, each one presenting new challenges and artistic explorations.

At the same time I work part-time as an imaging specialist for Twinsight, a company based in Grenoble in France, aiming to personalise and improve surgical care.

“The equilibrium of the HAT (Head+Arm+Trunk) during bipedal walking in baboons”. Published in: Druelle, F., Abourachid, A., Vasilopoulou-Kampitsi, M., Aerts, P. (2023). Convergence of Bipedal Locomotion: Why Walk or Run on Only Two Legs. In: Bels, V.L., Russell, A.P. (eds) Convergent Evolution. Fascinating Life Sciences. Springer, Cham. https://doi.org/10.1007/978-3-031-11441-0_14

Were you always going to be a scientist?

I’ve always felt a deep connection to nature, particularly animals and life itself. This connection and interest led me to choose biology as my first choice for my academic years. However, I never imagined during my early studies that I would eventually have the strength to go on to earn a PhD. It wasn’t a planned destination but I am very happy to have followed this path. It was a unique experience in the world of science.

“World map showing the distribution of current non-human great apes”. Published in: Archaéologia, No627, January 2024

And what about art – have you always enjoyed it?

Art was always a close second in my passions, even though I pursued it more as a personal exploration during my free time. I always enjoyed drawing and painting, with a particular focus on colors and forms. It wasn’t until later, after my studies, travels, and collaborations with fellow researchers, that naturalistic illustration became a part of my life. My work in science truly deepened my appreciation for the beauty of nature, and that’s when art and science naturally converged for me.

“Andrena gravida illustration” – digital illustration – personal project

What or who are your most important artistic influences?

Art museums have always been a source of inspiration for me. Over the years, I’ve explored many different artistic movements and eras, each offering different input to my artistic creations. My influences are a blend of several distinct styles—impressionism, the Bauhaus movement, and my personal favorite, surrealism. I have a deep admiration for the Bauhaus movement, particularly in its exploration of geometric forms and harmonic color combinations. To me, this approach is directly linked to the art we find in nature, where structure and color coexist in harmony.

I also find great inspiration in the naturalistic illustrators of the past, such as Ernst Haeckel. I like traditional techniques such as graphite and watercolor, that were mostly used to create illustrations long before the digital era.

“Compilation of animal species” – aquarelle on paper – commission

How do you make your art?

I use a variety of techniques in my artwork, depending on the preferences of the client. For book illustrations, I typically work with digital tools like Procreate, as these projects are long-term and require a significant amount of detailed work and multiple corrections. For shorter commissions, I often use traditional mediums like watercolor, pencil, or ink, watercolor being especially popular with clients. Personally, I enjoy working with pencil for black-and-white drawings, as well as oil paints for my personal pieces.

Regardless of the medium, my process always begins with an overall study of the subject in order to choose which aspects of its nature need to be emphasized. Once I have a clear vision, I create the initial sketch in graphite. From there, I build upon the drawing working in layers to bring out the depth and texture of the final piece.

“Pogona henrylawsoni” – aquarelle on paper – commission
“Papio anubis” – aquarelle on paper – commission

Does your science influence your art at all, or are they separate worlds?

Science has been a significant part of my life for many years, and it has undeniably influenced my art. The projects I’ve worked on, along with the methods of thinking, working, and experimenting in scientific research, have shaped my approach to drawing and expanded my imagination. However, although I often create naturalistic illustrations, I also enjoy exploring other subjects, such as emotions and surrealistic art.

What are you thinking of working on next?

I have several commissions lined up for later this fall and am always open to new collaborations. Since work in this field doesn’t come in regularly, I’m managing it part-time to balance my commitments. I would really love to, one day, expand my creations to the field of ceramics creating sculptures or other artistic objects that express my view of nature using the most natural material, clay.

“Papio anubis hind limb muskuloskeletal system” – digital illustration – comission

How/ where can people find more about you?

You can explore my latest illustration projects on both my website and Instagram, where I regularly update my portfolio. While my website shows a selection of projects, I use Instagram to provide a deeper look into my process and short commissions. There, I talk about techniques and tools I use, and offer followers a sneak peek at my latest creations.

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FocalPlane features… reproducibility in imaging

Posted by , on 15 October 2024

The next webinar in the FocalPlane features… series focuses on the important topic of reproducibility when acquiring, analysing and presenting imaging data. We are delighted to have talks from Helena Jambor and Kota Miura, who are both part of the QUAREP-LiMi (Quality Assessment and Reproducibility for Instruments and Images in Light Microscopy) community group, which aims to improve both quality assessment and quality control in microscopy. Helena’s talk will cover the community checklists for publishing images, which were developed by QUAREP-LiMi and published at the end of 2023, as she tells us ‘How not to lie with image data’. Kota’s talk will focus on bioimage analysis and the importance of reproducible analysis in ensuring scientific integrity.

Look out for our next FocalPlane features… webinar on 14 November 2024 at 14:30 GMT, which will showcase research from the Journal of Cell Science Special Issue: Imaging Cell Architecture and Dynamics. This webinar will be hosted by our Guest Editors Lucy Collinson and Guillaume Jacquemet and feature talks from Md Hashim Reza, Melanie Rug and Vikas Tillu.  

The recording of last month’s webinar on data management is now available to watch. You can also catch up on all past webinars on FocalPlane.

We’ll be planning our 2025 series soon, so please drop us an email at focalplane@biologists.com if you have any topics that you’d like us to cover.

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Development presents… October webinar on the development of ectoderm derivatives

Posted by , on 15 October 2024


On the topic of the development of ectoderm derivatives and chaired by Development’s Executive Editor, Katherine Brown.

Wednesday 30 October – 15:00 GMT

Eirini Maniou (University of Padua)
‘Quantifying tissue mechanics during neural tube morphogenesis’

Casey Griffin (New York University)
‘Teasing apart the role of SF34B4 in Nager syndrome: a tale of frogs and embryonic stem cells’

Daniel Aldea (Aix-Marseille University)
‘Melanocytes in development and rare skin diseases’

At the speakers’ discretion, the webinar will be recorded for viewing on demand. To see the other webinars scheduled in our series, and to catch up on previous talks, please visit: thenode.biologists.com/devpres

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From Scientist to Film Presenter: My part in creating a science documentary

Posted by , on 14 October 2024

When I began my PhD in 2020, I imagined my daily work would revolve around experiments, scientific writing, giving talks, and mentoring students. Little did I know that I’d soon be part of something quite different—a science documentary. I had the opportunity to collaborate with a team of scientists from the British Society for Developmental Biology (BSDB) and The Company of Biologists to create a film to promote UK developmental biology. As someone with no filmmaking experience, it was an exciting and daunting challenge. It was sure to be a steep learning curve full of science and fun.

We started by mapping our vision for the documentary. In film language, this included creating a film brief and storyboards. The storyboards laid out how each section of the documentary would unfold. We all pitched in with topic ideas, witling it down to four which would form our film vignettes: morphogenesis, cell migration, human brain development, and eye development. While it was hard to imagine at first how these ideas would translate into a polished documentary, we trusted the process. A crucial step in this was selecting a film production company. We wanted a collaborative team that could provide creative input whilst ensuring scientific accuracy. Fortunately, we teamed up with Cambridge Filmworks, who made the entire experience memorable.

My initial role in the documentary was to be an extra brain in the planning process. Especially since we were aiming to target a broad audience, including younger students. When the idea arose for me to help present the documentary, I couldn’t resist the opportunity. Despite my lack of on-camera experience, I’m passionate about science communication and developmental biology. The chance to work alongside brilliant scientists and collaborate with TV presenter and author Alice Roberts, who agreed to introduce and close the film, made the opportunity even more exciting.

Once we had our team of scientists and presenters on board, we focused on writing the script and drafting interview questions. The scientific team took the lead on script writing to give the documentary structure and direction. The script was then refined between our team and Cambridge Filmworks over numerous meetings and edits. This collaboration ensured that we included the relevant scientific information in a way that would be understood and be engaging to our audience. We also formulated questions which the scientists would answer throughout the documentary. The interview questions were designed to guide the scientists’ responses, keeping the flow natural and engaging. In most cases, multiple responses were filmed so that there were alternative options during the film editing process. We also had to ensure that the answers would be understood by a broad audience. With our scientific planning team and the filming team overlooking the filming, mistakes and jargon could be identified and corrected in the retakes.

Filming was an entirely new experience for me. On the first day, I learned what B-roll was and found myself awkwardly trying to walk naturally for the camera—it felt a bit robotic at first. As time progressed, I became much more confident and relaxed. I also watched Alice Roberts in action and took away some tips and tricks. During the latter stages of filming, I was recording voice over and performing solo pieces to camera, so I was thankful for the days leading up to this to hone the skills of presenting.

The main aim of the documentary is to showcase the wonder, importance, and applications of developmental biology. With that in mind, we invited a team of scientists who fitted into the selected vignettes. Helen Weavers kick starts the morphogenesis vignette by discussing her work on wound repair in fruit flies (Drosophila). Shankar Srinivas and Emily Noel follow with insights into how a small cluster of cells transforms into the complex, functioning heart. Tom Bennett rounds off the vignette by discussing plant development, offering a fascinating comparison between plant and animal development.

Behind the scenes of Courtney, this post’s author (left), speaking to Helen Weavers (right).

Next, the documentary explores neural crest cells, chosen for their remarkable migration abilities and their capacity to differentiate into a wide range of cell types. Karen Liu talks about the origin and migration of neural crest cells. We then have Elena Scarpa who brings a mechanical angle to the topic and highlights neural crest cell derived cancers.

In our vignette on human brain development, Katie Long explains how the brain folds during embryonic development and the differences between identical twins’ brains. My identical twin, Chloe Lancaster, joined the documentary to add a real-life twinning element. Laura Pellegrini discusses brain evolution and expresses her fascination for understanding the uniqueness of human brain development. She also introduces the audience to organoids and highlights their valuable contribution to understanding human development. 

Finally, we dive into eye development with Pete Coffey and Rodrigo Young. Pete talks about his lab’s contribution to the treatment of eye diseases. His team has managed to surgically replace cells in the back of the eye with lab grown cells which enabled a patient to regain sight. He was taking a patch of cells to a patient on the day of filming which he was very excited about! Rodrigo delves into eye development with a key element being how two eyes of the same size and shape develop independently from each other.

What surprised me most during the filming process was how naturally the scientists adapted to the camera, despite not being accustomed to it. It turns out that all those conference presentations prepared them well for documentary interviews.

With the filming complete, it was time for Cambridge Filmworks to put the pieces together. Without being intimately involved in the editing, I can only say that this was some sort of self-organisation with constant feedback between the film editor and our team. We were also lucky enough to be invited to the Cambridge Studio to view the movie and provide feedback. It was surreal to see all the filming in a documentary for the first time.

I have been incredibly lucky to be involved in such a fun and exciting project with an incredible team of scientists and film creators. Brainstorming ideas and then seeing them in action during filming and editing was extremely rewarding, but the most valuable part of this experience has been all the people I met along the way. I interacted with scientists from all over the UK and picked their brains about different topics, science and career related. I might not have crossed paths with such inspiring people if I had not stepped out of the lab to embark on something completely different from my day-to-day scientific life. We also had a lot of fun along the way, laughing through retakes and mishaps.

I hope you enjoy watching the documentary as much as we enjoyed creating it. Please share it with your friends, family, and colleagues—let’s inspire the next generation of developmental biologists!

Film team: Adam Giles, Rich Millen, Barrie White, Douglas Murchie, Zheko Georgiev, Nigel Kinnings, Bronwyn Rand

Scientific planning team: Paul Martin, Shankar Srinivas, Katherine Brown, Rodrigo Young, Jeremy Green, Courtney Lancaster with help and funding from the BSDB and The Company of Biologists

Interviewees: Helen Weavers, Shankar Srinivas, Emily Noel, Tom Bennett, Karen Liu, Elena Scarpa, Katie Long, Laura Pellegrini, Pete Coffey, Rodrigo Young

Presenters + more: Alice Roberts, Courtney Lancaster, Chloe Lancaster

The documentary can be watched on YouTube:

Full length (28 minutes): https://youtu.be/avrmIs3vPUQ

Short version (15 minutes): https://youtu.be/LlkVDYc8CgI

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Categories: Societies, Video

Fantastic academic jobs and how to find them 🪄

Posted by , on 10 October 2024

At preLights, the preprint highlighting service run by early-career researchers, we recently launched ‘spotLights’: a podcast series where we delve deeper into exciting preprints, featuring interviews with the scientists behind the research. These conversations allow you to hear the stories, challenges, and motivations that go into producing impactful scientific work. All episodes so far can be found here.

In episode three of spotLights, we explore a topic that’s highly relevant for early-career researchers: An updated and expanded characterization of the biological sciences academic job market. If you’re on the academic job hunt or thinking about your next steps in academia, this episode is full of valuable insights!

It goes beyond the accompanying preLight post that describes the key findings. You’ll get the chance to hear from two of the preprint authors, Dr Nafisa Jadavji and Dr Chris Smith, who share the background, process, and motivations behind their study. Don’t miss out on this insightful discussion! Tune in now and get the inside scoop directly from the experts.

Note: spotLights episodes 1 and 2 both revolve around preprinted work that describes the cephalic furrow as a “crumble zone” between head and trunk tissues (see related preLight post). Episode 1 features Bruno Vellutini, the first author of the preprint “Patterned embryonic invagination evolved in response to mechanical instability.” Episode 2 highlights the work led by Bipasha Dey, Verena Kaul, and Girish Kale, resulting in the preprint “Divergent evolutionary strategies preempt tissue collision in fly gastrulation.”

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September in preprints

Posted by , on 9 October 2024

Welcome to our monthly trawl for developmental and stem cell biology (and related) preprints.

The preprints this month are hosted on bioRxiv and arXiv – use these links below to get to the section you want:

Developmental biology

Cell Biology

Modelling

Tools & Resources

Research practice and education

Developmental biology

| Patterning & signalling

Wnt5a and Notum Influence the Temporal Dynamics of Cartilaginous Mesenchymal Condensations in Developing Trachea

Natalia Bottasso-Arias, Megha Mohanakrishnan, Sarah Trovillion, Kaulini Burra, Nicholas X. Russell, Yixin Wu, Yan Xu, Debora Sinner

Melastatin subfamily Transient Receptor Potential channels support spermatogenesis in planarian flatworms

Haley Nicole Curry, Roger Huynh, Labib Rouhana

Wnt/β-catenin signalling underpins juvenile Fasciola hepatica growth and development

Rebecca Armstrong, Nikki J Marks, Timothy G Geary, John Harrington, Paul M Selzer, Aaron G Maule

Axin1 and Axin2 regulate the WNT-signaling landscape to promote distinct mesoderm programs

Rocío Hernández-Martínez, Sonja Nowotschin, Luke T.G. Harland, Ying-Yi Kuo, Bart Theeuwes, Berthold Göttgens, Elizabeth Lacy, Anna-Katerina Hadjantonakis, Kathryn V. Anderson

Developmental Effects of Lithium and Zinc in Planaria

Alberto Molano

Three waves of ovarian follicles in juvenile mice

Qi Yin, Allan C Spradling

Cardiac Fibroblasts regulate myocardium and coronary vasculature development via the collagen signaling pathway

Yiting Deng, Yuanhang He, Juan Xu, Haoting He, Manling Zhang, Guang Li

Cell type and cell signaling innovations underlying mammalian pregnancy

Daniel J. Stadtmauer, Silvia Basanta Martínez, Jamie D. Maziarz, Alison G. Cole, Gülay Dagdas, Gilbecca Rae Smith, Frank van Breukelen, Mihaela Pavličev, Günter P. Wagner

Phosphorylation-induced SUMOylation promotes Ulk4 condensation at ciliary tip to transduce Hedgehog signal

Mengmeng Zhou, Yuhong Han, Jin Jiang

Non-cell-autonomous control of gastruloid development by the lncRNA T-UCstem1 through DKK1-dependent modulation of WNT signalling

Arianna Coppola, Filomena Amoroso, Federica Saracino, Gennaro Andolfi, Edoardo Sozzi, Paolo Salerno, Pietro Zoppoli, Alessandro Fiorenzano, Giuseppe Merla, Eduardo Jorge Patriarca, Gabriella Minchiotti, Annalisa Fico

Human macula formation involves two waves of retinoic acid signaling suppression via CYP26A1 regulating cell cycle exit and cone specification

Philippa Harding, Maja Wojtynska, Alexander J. Smith, Robin R. Ali, Rachael A. Pearson

The Gq/11 family of Gα subunits is necessary and sufficient for lower jaw development

Stanley M. Kanai, Chloe R. Garcia, MaCalia R. Augustus, Shujan A. Sharafeldeen, Elliott P. Brooks, Juliana Sucharov, Ezra S. Lencer, James T. Nichols, David E. Clouthier

Notch/Hairless Pathway Modulation of sog Transcriptional Bursting in Prelude to Gastrulation

Kelli D. Fenelon, Priyanshi Borad, Biraaj Rout, Parisa Boodaghimalidarreh, Mohammad S. Nasr, Jacob Luber, Theodora Koromila

Notch and LIM-homeodomain protein Arrowhead regulate each other in a feedback mechanism to play a role in wing and neuronal development in Drosophila

Jyoti Singh, Dipti Verma, Bappi Sarkar, Maimuna Sali Paul, Mousumi Mutsuddi, Ashim Mukherjee

From Singh et al. This image is made available under a CC-BY 4.0 International license.

YAP/TAZ signaling in allantois-derived cells is required for placental vascularization

Siqi Gao, Triloshan Thillaikumaran, Martin H. Dominguez, William Giang, Kevin Hayes, Xiaowen Chen, Jesse Pace, Jenna Bockman, Danielle Jathan, Derek Sung, Sweta Narayan, Maxwell Frankfurter, Patricia Mericko-Ishizuka, Jisheng Yang, Marco Castro, Michael Potente, Mark L. Kahn

Spatiotemporal Patterns of Gene Expression During Development of a Complex Colony Morphology

Gareth A. Cromie, Zhihao Tan, Michelle Hays, Amy Sirr, Aimée M. Dudley

Somatic Transthyretin-Related Proteins in C. elegans Govern Reproductive Longevity by Sustaining Sperm Integrity and Timely Ovulation

Tingshan Wu, Haochen Lyu, Zhao Wang, Zhaoyang Jiang, Yingchuan B. Qi

Dynamic BMP signaling regulates sclerotome induction and lineage diversification in zebrafish

Linjun Xie, Roger C. Ma, Katrinka M. Kocha, Emilio E. Méndez-Olivos, Peng Huang

AKT-mediated phosphorylation of TSC2 controls stimulus- and tissue-specific mTORC1 signaling and organ growth

Yann Cormerais, Samuel C. Lapp, Krystle C. Kalafut, Madi Y. Cissé, Jong Shin, Benjamin Stefadu, Jean Personnaz, Sandra Schrotter, Angelica D’Amore, Emma R. Martin, Catherine L. Salussolia, Mustafa Sahin, Suchithra Menon, Vanessa Byles, Brendan D. Manning

| Morphogenesis & mechanics

Tensions on the actin cytoskeleton and apical cell junctions in the C. elegans spermatheca are influenced by spermathecal anatomy, ovulation state and activation of myosin

Fereshteh Sadeghian, Noa W.F. Grooms, Samuel H. Chung, Erin J. Cram

Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulation

Juan Manuel Gomez, Carlo Bevilacqua, Abhisha Thayambath, Maria Leptin, Julio M Belmonte, Robert Prevedel

Mast cells are not essential for pubertal mammary gland branching

Simran Kapoor, Jimmy Marsden, Clara M. Munz, Cyril Carvalho, Marlene Magalhaes Pinto, Bert Malengier-Devlies, Solvig Becker, Guillaume Seuzaret, Katelyn Patatsos, Ramazan Akyol, Marc Dalod, Amy Pedersen, Gillian Wilson, Rebecca Gentek

Nodal Modulator (NOMO) is a force-bearing transmembrane protein required for muscle differentiation

Brigitte S Naughton, Swapnil C Devarkar, Sunanda Mallik, Stacey Oxendine, Sanjana Junnarkar, Yuan Ren, Vanessa Todorow, Julien Berro, Janine Kirstein, Yong Xiong, Christian Schlieker

PIEZO-dependent mechano-sensing of the niche is essential for intestinal stem cell fate decision and maintenance

Meryem B. Baghdadi, Ronja M. Houtekamer, Louisiane Perrin, Abilasha Rao-Bhatia, Myles Whelen, Linda Decker, Martin Bergert, Carlos Pérez-Gonzàlez, Réda Bouras, Giacomo Gropplero, Adrian KH Loe, Amin Afkhami-Poostchi, Xin Chen, Xi Huang, Stephanie Descroix, Jeffrey L. Wrana, Alba Diz-Muñoz, Martijn Gloerich, Arshad Ayyaz, Danijela Matic Vignjevic, Tae-Hee Kim

Mouse nephron formation is impaired by moderate-dose arsenical exposure

Carlos Agustin Isidro Alonso, Jenna Haverfield, Gabriela Regalado, Sihem Sellami, Natascha Gagnon, Ajay Rajaram, Pierre Olivier Fiset, Aimee K Ryan, Koren K Mann, Indra R Gupta

Control of alveolar bone development, homeostasis, and socket healing by salt inducible kinases

Nicha Tokavanich, Byron Chan, Katelyn Strauss, Christian D. Castro Andrade, Yuki Arai, Mizuki Nagata, Marc Foretz, Daniel J. Brooks, Noriaki Ono, Wanida Ono, Marc N. Wein

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells

Kaitlin P. McCreery, Aki Stubb, Rebecca Stephens, Nadezda A. Fursova, Andrew Cook, Kai Kruse, Anja Michelbach, Leah C. Biggs, Adib Keikhosravi, Sonja Nykänen, Christel Hydén-Granskog, Jizhong Zou, Jan-Wilm Lackmann, Carien M. Niessen, Sanna Vuoristo, Yekaterina A. Miroshnikova, Sara A. Wickström

Robinow Syndrome DVL1 mutations disrupt morphogenesis and appendage formation in a Drosophila disease model

Gamze Akarsu, Katja R MacCharles, Kenneth Kin Lam Wong, Joy Richman, Esther M. Verheyen

Substrate stiffness regulates neuronal maturation via Piezo1-mediated TTR activity

Eva Kreysing, Helene Gautier, Robert J Humphrey, Katrin A Mooslehner, Leila A Muresan, Daniel Haarhoff, Sudipta Mukherjee, Xiaohui X Zhao, Alex K Winkel, Andrea Dimitracopoulos, Eva K Pillai, Ragnhildur Thora Karadottir, Kristian Franze

Calreticulin is required for cuticle deposition and trabeculae formation inside butterfly wing scale cells

Ru Hong, Cedric Finet, Antonia Monteiro

Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud

Min Shi, Brittney Crouse, Nambirajan Sundaram, Naomi Pode Shakked, Lioba Ester, Weitao Zhang, Vinothini Janakiram, Raphael Kopan, Michael A. Helmrath, Joseph V. Bonventre, Kyle W. McCracken

Local optogenetic NMYII activation within the zebrafish neural rod results in long-range, asymmetric force propagation

Helena A Crellin, Chengxi Zhu, Guillermo Serrano-Nájera, Amelia Race, Kevin O’Holleran, Martin O Lenz, Clare E Buckley

Centralspindlin promotes C. elegans anchor cell specification, vulva induction and morphogenesis

Tatsuya Kato, Olga Skorobogata, Christian Rocheleau

Microtubules coordinate mitochondria transport with myofibril morphogenesis during muscle development

Jerome Avellaneda, Duarte Candeias, Ana da Rosa Soares, Edgar R. Gomes, Nuno Miguel Luis, Frank Schnorrer

From Avellaneda et al. This image is made available under a CC-BY-ND 4.0 International license.

Mechanical coupling of compartments drives polarity and patterning of mouse auditory epithelium

Anubhav Prakash, Sukanya Raman, Raman Kaushik, Anton S Iyer, Raj K. Ladher

ARHGEF3 Regulates Hair Follicle Morphogenesis

Krithika Kalyanakrishnan, Amy Beaudin, Alexandra Jetté, Sarah Ghezelbash, Diana Ioana Hotea, Jie Chen, Philippe Lefrançois, Mélanie Laurin

The pericardium forms as a distinct structure during heart formation

Hannah R. Moran, Obed O. Nyarko, Rebecca O’Rourke, Ryenne-Christine K. Ching, Fréderike W. Riemslagh, Brisa Peña, Alexa Burger, Carmen C. Sucharov, Christian Mosimann

Mechanical regulation of cuboidal-to-squamous epithelial transition in the Drosophila developing wing

Stefan Harmansa, Thomas Lecuit

| Genes & genomes

Dynamic allelic expression in mouse mammary gland across the adult developmental cycle

Geula Hanin, Kevin R Costello, Hugo Tavares, Shrina Patel, Anne C Ferguson-Smith

The Hox Gene, abdominal A controls timely mitotic entry of neural stem cell and their growth during CNS development in Drosophila

Papri Das, Smrithi Murthy, Eshan Abbas, Kristin White, Richa Arya

Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development

Alexandra de la Porte, Julia Schröder, Moritz Thomas, Johanna Geuder, Michael Sterr, Xavier Pastor, Leslie E. Sanderson, Tahsin Stefan Barakat, Wolfgang Enard, Carsten Marr, Christian Schröter, Micha Drukker

The Transcription Factor TCF21 is necessary for adoption of cell fates by Foxd1+ stromal progenitors during kidney development

Gal Finer, Mohammad D. Khan, Yalu Zhou, Gaurav Gadhvi, George S. Yacu, Joo-Seop Park, R. Ariel Gomez, Maria Luisa Sequeira-Lopez, Susan E. Quaggin, Deborah R. Winter

The Pax transcription factor EGL-38 links EGFR signaling to assembly of a cell-type specific apical extracellular matrix in the Caenorhabditis elegans vulva

Helen F. Schmidt, Chelsea B. Darwin, Meera V. Sundaram

The Drosophila myogenic inhibitor Him gene is essential for adult muscle function and muscle stem cell maintenance

Robert Mitchell-Gee, Robert Hoff, Kumar Vishal, Daniel Hancock, Sam McKitrick, Cristina Newnes-Querejeta, TyAnna L. Lovato, Richard M. Cripps, Michael V. Taylor

Pcbp1 constrains Oct4 expression in the context of pluripotency

E. I. Bakhmet, A. S. Zinovyeva, A. A. Kuzmin, D. V. Smirnova, M. N. Gordeev, E. E. Petrenko, N. D. Aksenov, A. N. Tomilin

Deciphering the Cell-Specific Transcript Heterogeneity and Alternative Splicing during the Early Embryonic Development of Zebrafish

Xiumei Lin, Xue Wang, Chang Liu, Chuanyu Liu, Tao Zeng, Ziqi Yuan, Meidi Hu, Rong Xiang, Kaichen Zhao, Jie Zhou, Shichen Yang, Yang Wang, Kaifeng Meng, Hui Wang, Guangli He, Rui Zhao, Jiaheng Liu, Yunqi Huang, Jingfang Pan, Jialu Wang, Junyi Chen, Fei Guo, Yuliang Dong, Xun Xu, Daji Luo, Ying Gu, Longqi Liu, Zhiqiang Dong, Liang Chen

Epigenetic priming of embryonic enhancer elements coordinates developmental gene networks

Christopher D Todd, Jannat Ijaz, Fereshteh Torabi, Oleksandr Dovgusha, Stephen Bevan, Olivia Cracknell, Tim Lohoff, Stephen Clark, Ricard Argelaguet, Juliette Pearce, Ioannis Kafetzopoulos, Alice Santambrogio, Jennifer Nichols, Ferdinand von Meyenn, Ufuk Guenesdogan, Stefan Schoenfelder, Wolf Reik

From Todd et al. This image is made available under a CC-BY 4.0 International license.

Cell-type-specific splicing of transcription regulators and Ptbp1 by Rbfox1/2/3 in the developing neocortex

Xiangbin Ruan, Kaining Hu, Yalan Yang, Runwei Yang, Elizabeth Tseng, Bowei Kang, Aileen Kauffman, Rong Zhong, Xiaochang Zhang

The chromatin remodeler DEK promotes proliferation of mammary epithelium and is associated with H3K27me3 epigenetic modifications

Megan Johnstone, Ashley Leck, Taylor Lange, Katherine Wilcher, Miranda S. Shephard, Aditi Paranjpe, Sophia Schutte, Susanne Wells, Ferdinand Kappes, Nathan Salomonis, Lisa M. Privette Vinnedge

Autism and Intellectual Disability-Associated MYT1L Mutation Alters Human Cortical Interneuron Differentiation, Maturation, and Physiology

Ramachandran Prakasam, Julianna Determan, Mishka Narasimhan, Renata Shen, Maamoon Saleh, Gareth Chapman, Komal Kaushik, Paul Gontarz, Kesavan Meganathan, Bilal Hakim, Bo Zhang, James E Huettner, Kristen L Kroll

Dynamics of recombination, X inactivation and centromere proteins during stick insect spermatogenesis

William Toubiana, Zoe Dumas, Marie Delattre, Tanja Schwander

Robust Binding Capability and Loss of Telomere-Binding Proteins Underlying the Evolution of Telomeric Repeat Motifs

Hobum Song, Seonhong Kim, Daisy S. Lim, Hee-Jung Choi, Junho Lee

Optimized CRISPR inhibition and activation opens key avenues for systematic biological exploration in zebrafish

Nelson B. Barrientos, Elyse A. Shoppell, Rachel J. Boyd, Valeria C. Culotta, Andrew S. McCallion

Sex-, development-, and nutrition-dependent expression of a major age-at-maturity gene in Atlantic salmon

Eirik Ryvoll Åsheim, Paul Vincent Debes, Andrew House, Petra Liljeström, Annukka Ruokolainen, Morgane Frapin, Iikki Donner, Ehsan Pashay Ahi, Jaakko Erkinaro, Jukka-Pekka Verta, Craig R Primmer

De novo assembly of the selfish t supergene reveals a deleterious evolutionary trajectory

Jan-Niklas Runge, Kristian Ullrich, Anna K. Lindholm

The Drosophila maternal-effect gene abnormal oocyte (ao) does not repress histone gene expression

Risa Takenaka, Sierra M. Simmerman, Casey A. Schmidt, Eric H. Albanese, Leila E. Rieder, Harmit S. Malik

The Primate Major Histocompatibility Complex: An Illustrative Example of Gene Family Evolution

Alyssa Lyn Fortier, Jonathan K. Pritchard

Evolutionary genomics predicts adaptive genetic and plastic gene expression responses to climate change in a key alpine forest tree species

Zhiqin Long, Yupeng Sang, Jiajun Feng, Xinxin Zhang, Tingting Shi, Lushui Zhang, Kangshan Mao, Loren H. Rieseberg, Jianquan Liu, Jing Wang

Dynamics of recombination, X inactivation and centromere proteins during stick insect spermatogenesis

Zoé Dumas, William Toubiana, Marie Delattre, Tanja Schwander

From Dumas et al. This image is made available under a CC-BY-NC-ND 4.0 International license.

Expansion of Drosophila haemocytes using a conditional GeneSwitch driver affects larval haemocyte function, but does not modulate adult lifespan or survival from infection

Dan J Hayman, Lola M Morrin, Sudipta Halder, Eleanor J Phillips, Mirre J P Simons, Iwan R Evans

Developmental transcriptomics in Pristionchus reveals the logic of a plasticity gene regulatory network

Shelley Reich, Tobias Loschko, Julie Jung, Samantha Nestel, Ralf J. Sommer, Michael S. Werner

The L1CAM SAX-7 is an antagonistic modulator of Erk Signaling

Melinda Moseley-Alldredge, Caroline Aragón, Marcus Vargus, Divya Alley, Nirali Somia, Lihsia Chen

NR2F2 regulation of interstitial to fetal Leydig cell differentiation in the testis: insights into differences of sex development

Martín Andrés Estermann, Sara Grimm, Abigail Kitakule, Karina Rodriguez, Paula Brown, Kathryn McClelland, Ciro Amato, Humphrey Hung-Chang Yao

Enhancer adoption by an LTR retrotransposon generates viral-like particles causing developmental limb phenotypes

Juliane Glaser, Giulia Cova, Beatrix Fauler, Cesar A. Prada-Medina, Virginie Stanislas, Mai H.Q. Phan, Robert Schöpflin, Yasmin Aktas, Martin Franke, Guillaume Andrey, Christina Paliou, Verena Laupert, Wing-Lee Chan, Lars Wittler, Thorsten Mielke, Stefan Mundlos

Gene regulatory dynamics during craniofacial development in a carnivorous marsupial

Laura E. Cook, Charles Y. Feigin, John Hills, Davide M. Vespasiani, Andrew J. Pask, Irene Gallego Romero

Effects of alcohol on the transcriptome, methylome, and metabolome of in vitro gastrulating human embryonic cells

Essi Wallen, Karita Ramo, Jussi Vehvilainen, Joonas Sokka, Marko Lehtonen, Timo Otonkoski, Ras Trokovic, Pauliina Auvinen, Olli Karkkainen, Nina Kaminen-Ahola

Live imaging human embryos reveals mitotic errors and lineage specification prior to implantation

Ahmed Abdelbaki, Afshan McCarthy, Anita Karsa, Leila Muresan, Kay Elder, Athanasios Papathanasiou, Phil Snell, Leila Christie, Martin Wilding, Benjamin J. Steventon, Kathy K. Niakan

Genomic mosaicism reveals developmental organization of trunk neural crest-derived ganglia

Keng Ioi Vong, Yanina D. Alvarez, Geoffroy Noel, Scott T. Barton, Changuk Chung, Robyn Howarth, Naomi Meave, Qingquan Zhang, Fiza Jiwani, Chelsea Barrows, Arzoo Patel, Jiang Xiong Wang, Neil Chi, Stephen F. Kingsmore, Melanie D. White, Xiaoxu Yang, Joseph G. Gleeson

Dynamic transcriptional and epigenetic changes define postnatal tendon growth

Heather L. Dingwall, Mor Grinstein, Terence D. Capellini, Jenna L. Galloway

Elavl1 is dispensable for appendicular skeletal development

Rohini Parsha, Satya K. Kota

Fast-twitch myofibrils grow in proportion to Mylpf dosage in the zebrafish embryo

Tayo E Adekeye, Emily M Teets, Emily A Tomak, Sadie L Waterman, Kailee A Sprague, Angelina White, Maddison L Coffin, Sabrina M Varga, Teresa E Easterbrooks, Sarah J Shepherd, Jared D Austin, Dmitrii Krivorotko, Troy E Hupper, Joshua B Kelley, Sharon L Amacher, Jared C Talbot

LINE1 promotes nuclear compartmentalization to repress the 8-cell state in embryonic stem cells

Juan Zhang, Lamisa Ataei, Liang Wu, Kirti Mittal, Linh Huynh, Shahil Sarajideen, Abdul Mazid, David P. Cook, Daniel Trcka, Kevin Tse, Jeffrey L. Wrana, Michael M. Hoffman, Miguel A. Esteban, Miguel Ramalho-Santos

Distinct Roles for SETα and SETβ in Early Cell Fate Decisions

Patrick Siang Lin Lim, Eran Meshorer

Highly conserved sequence-specific double-stranded DNA binding networks contributing to divergent genomic evolution of human and chimpanzee brain development

Gennadi Glinsky

| Stem cells, regeneration & disease modelling

Ciliary marginal zone of the developing human retina maintains retinal progenitor cells until late gestational stages

Kiara C. Eldred, Sierra J. Edgerton, Isabel Ortuño-Lizarán, Juliette Wohlschlegel, Stephanie M. Sherman, Sidnee Petter, Gracious Wyatt-Draher, Dawn Hoffer, Ian Glass, Anna La Torre, Thomas A. Reh

The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells

Julia Ye, Ryan M. Boileau, Ronald J. Parchem, Robert L. Judson-Torres, Robert Blelloch

An RNA binding regulatory cascade controls the switch from proliferation to differentiation in the Drosophila male germ line stem cell lineage

Devon E. Harris, Jongmin J. Kim, Sarah R. Stern, Hannah M. Vicars, Neuza R. Matias, Lorenzo Gallicchio, Catherine C. Baker, Margaret T. Fuller

Secretome analysis by proximal labeling in Golgi apparatus for identifying fat body derived humoral factors in Drosophila disc regeneration

Yutaka Yoshida, Soshiro Kashio, Masayuki Miura

The Initiator Caspase Dronc Drives Compensatory Proliferation of Apoptosis-Resistant Cells During Epithelial Tissue Regeneration After Ionizing Radiation

Tslil Braun, Naama Afgin, Lena Sapozhnikov, Ehud Sivan, Andreas Bergmann, Luis Alberto Baena-Lopez, Keren Yacobi-Sharon, Eli Arama

Organoid Modeling and Single-Cell Profiling Uncover the Migration Mechanism of Smooth Muscle Cells in Moyamoya Disease

Shihao He, Junze Zhang, Xilong Wang, Zhen Qi, Zhenyu Zhou, Yanru Wang, Shaoqi Xu, Dandan Li, Xun Ye, Ziqi Liu, Xiaokuan Hao, Yuanli Zhao, Rong Wang

Culture Conditions Differentially Regulate the Inflammatory Niche and Cellular Phenotype of Tracheo-Bronchial Basal Stem Cells

Shubha Murthy, Denise A. Seabold, Lalit K. Gautam, Adrian M. Caceres, Rosemary Sease, Ben A. Calvert, Shana Busch, Aaron Neely, Crystal N. Marconett, Amy L. Ryan

Simultaneous generation of osteoblasts and adipocytes by a bivalent differentiation medium from bone marrow stromal/stem cells (BMSCs)

Dawei Qiu, Dong Liu, Jia Chen, Jingwen Huang, Yong Yang, Wanyi Wei, Ziwei Luo

Proliferation and differentiation of intestinal stem cells depends on the zinc finger transcription factor BCL11/Chronophage

Siamak Redhai, Nick Hirschmüller, Tianyu Wang, Shivohum Bahuguna, Svenja Leible, Stefan Peidli, Erica Valentani, Sviatoslav Kharuk, Michaela Holzem, Lea Bräckow, Fillip Port, David Ibberson, Wolfgang Huber, Michael Boutros

Gonadotrophs have a dual origin, with most derived from pituitary stem cells during minipuberty

Daniel Sheridan, Probir Chakravarty, Gil Golan, Yolanda Shiakola, Christophe Galichet, Patrice Mollard, Philippa Melamed, Robin Lovell-Badge, Karine Rizzoti

Hypoxia and loss of GCM1 expression prevents differentiation and contact inhibition in human trophoblast stem cells

Jessica K. Cinkornpumin, Sin Young Kwon, Anna-Maria Prandstetter, Theresa Maxian, Jacinthe Sirois, James Goldberg, Joy Zhang, Deepak Saini, Purbasa Dasgupta, Mariyan J. Jeyarajah, Stephen Renaud, Soumen Paul, Sandra Haider, William A Pastor

MIXL1 Activation in Endoderm Differentiation of Human Induced Pluripotent Stem Cells

Pierre Osteil, Sarah Withey, Nicole Santucci, Nader Aryamanesh, Chi Nam Ignatius Pang, Nazmus Salehin, Jane Sun, Annie Qin, Jiayi Su, Hilary Knowles, Zhaoxiang Cai, Xiucheng Bella Li, Ernst J Wolvetang, Patrick P.L. Tam

Modulating DNA Polα Enhances Cell Reprogramming Across Species

Rajesh Ranjan, Binbin Ma, Ryan J. Gleason, Yijun Liao, Yingshan Bi, Brendon E. M. Davis, Guanghui Yang, Maggie Clark, Vikrant Mahajan, Madison Condon, Nichole A. Broderick, Xin Chen

Single Cell Profiling in the Sox10Dom/+ Hirschsprung Mouse Implicates Hoxa6 in Enteric Neuron Lineage Allocation

Justin A. Avila, Joseph T. Benthal, Jenny C. Schafer, E. Michelle Southard-Smith

RFX3 is essential for the development and maturation of human pancreatic islets derived from pluripotent stem cells

Bushra Memon, Noura Aldous, Ahmed K. Elsayed, Sadaf Ijaz, Sikander Hayat, Essam M. Abdelalim

Identification of neurodevelopmental organization of the cell populations of juvenile Huntington’s disease using dorso-ventral HD organoids and HD mouse embryos

Karolina Świtońska-Kurkowska, Jakub Kubiś, Joanna Delimata-Raczek, Bart Krist, Magda Surdyka, Żaneta Kalinowska-Pośka, Piotr Piasecki, Luiza Handschuh, Jan Podkowiński, Magdalena Rakoczy, Anna Samelak-Czajka, Michael Hayden, Nicholas S Caron, Maciej Figiel

From Świtońska-Kurkowska et al. This image is made available under a CC-BY 4.0 International license.

Reducing oxidative stress improves ex vivo polymer-based human haematopoietic stem and progenitor cell culture and gene editing

Yavor Bozhilov, Elizabeth Brown, Ian Hsu, Indranil Singh, Alejo Rodriguez-Fraticelli, Anindita Roy, Satoshi Yamazaki, Adam C. Wilkinson

MEK1/2 kinases cooperate with c-Myc:MAX to prevent polycomb repression of TERT in human pluripotent stem cells

Spandana Kotian, Lindsay F. Rizzardi, Josh L. Stern

Human iPSC-derived brain pericytes exhibit differences in inflammatory activation compared to primary human brain pericytes

Samuel JC McCullough, Eliene Albers, Akshata Anchan, Jane Yu, Simon J O’Carroll, Bronwen Connor, E Scott Graham

Linker histone regulates the myeloid versus lymphoid bifurcation of multipotent hematopoietic stem and progenitors

Kutay Karatepe, Bruna Mafra de Faria, Jian Zhang, Xinyue Chen, Hugo Pinto, Dmitry Fyodorov, Esen Sefik, Michael Willcockson, Richard Flavell, Arthur Skoultchi, Shangqin Guo

Early lineage segregation of primary myotubes from secondary myotubes and adult muscle stem cells

Gauthier Toulouse, William Jarassier, Valérie Morin, Fabien Le Grand, Christophe Marcelle

Inflammation perturbs hematopoiesis by remodeling specific compartments of the bone marrow niche

James W. Swann, Ruiyuan Zhang, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Xiaonan Wang, Melissa A. Proven, Peter T. Shyu, X. Edward Guo, Berthold Göttgens, Emmanuelle Passegué

Metabolic reprogramming regulates histone lactylation during zebrafish caudal fin regeneration

Jorge Borbinha, Raquel Lourenco, Ana Sofia Brandao, Ana Carvalho, Rune Matthiesen, Antonio Jacinto

Differential responses and recovery dynamics of HSPC populations following Plasmodium chabaudi infection.

Federica Bruno, Christiana Georgiou, Deirdre Cunningham, Samantha Atkinson, Lucy Bett, Marine Secchi, Flora Birch, Sara Gonzalez Anton, jean langhorne, Cristina Lo Lo Celso

Systemic deficits in lipid homeostasis promote aging-associated impairments in B cell progenitor development

Silvia Vicenzi, Fangyuan Gao, Parker Côté, Joshua D. Hartman, Lara C. Avsharian, Ashni A. Vora, R. Grant Rowe, Hojun Li, Dorota Skowronska-Krawczyk, Leslie A. Crews

Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells

Rachel Wellington, Xiaoyi Cheng, Clyde A. Campbell, Cole Trapnell, Raquel Espin-Palazon, Brandon Hadland, Sergei Doulatov

Bnip3lb-driven mitophagy sustains expansion of the embryonic hematopoietic stem cell pool

Eleanor Meader, Morgan T. Walcheck, Mindy R. Leder, Ran Jing, Paul J. Wrighton, Wade W. Sugden, Mohamad A. Najia, Isaac M. Oderberg, Vivian M. Taylor, Zachary C. LeBlanc, Eleanor D. Quenzer, Sung-Eun Lim, George Q. Daley, Wolfram Goessling, Trista E. North

Apurinic/apyrimidinic endodeoxyribonuclease 2 (APEX2/APE2) is required for efficient expression of TERT in human embryonic stem cells

Josh L. Stern, Lindsay F. Rizzardi, Natalie R. Gassman

Sox9 prevents retinal degeneration and is required for limbal stem cell differentiation in the adult mouse eye

Alicia Hurtado, Víctor López-Soriano, Miguel Lao, M. Ángeles Celis-Barroso, Pilar Lazúen, Alejandro Chacón de Castro, Yolanda Ramírez-Casas, Miguel Alaminos, J. Martin Collinson, Miguel Burgos, Rafael Jiménez, F. David Carmona, Francisco J. Barrionuevo

Neuroendocrine Control of Intestinal Regeneration Through the Vascular Niche in Drosophila

André B. Medina, Jessica Perochon, Cai Johnson, Sofia Polcowñuk, Yuanliangzi Tian, Yachuan Yu, Julia B. Cordero

From Medina et al. This image is made available under a CC-BY 4.0 International license.

| Plant development

Raffinose induces autophagy to promote plant growth

Sahar Magen, Sahar Daniel, Shahar Weiss, David J. Factor, Sergey Mursalimov, Yoram Soroka, Simon Michaeli, Tamar Avin-Wittenberg

ETTIN-mediated auxin signalling is an angiosperm-specific neofunctionalization for carpel development

Aaron Chun Hou Ang, Sumanth Mutte, Dolf Weijers, Lars Østergaard

Evolutionary conserved RLF, a plant cytochrome b5-like heme-binding protein, is essential for organ development in Marchantia polymorpha

Kentaro P. Iwata, Takayuki Shimizu, Yuuki Sakai, Tomoyuki Furuya, Hinatamaru Fukumura, Yuki Kondo, Tatsuru Masuda, Kimitsune Ishizaki, Hidehiro Fukaki

Deciphering transcriptional programming during lupin (Lupinus angustifolius) seed development using RNA-seq

Virginia Wainaina, Tina Rathjen, Trijntje Hughes, Annelie Marquardt, Natalie Fletcher, Hayley Casarotto, Meredith McNeil, Kerensa McElroy, Ling-Ling Gao

CUP-SHAPED COTYLEDON2 activates MIR319C transcription and promotes cell proliferation in Arabidopsis leaf primordia

Naveen Shankar, Abhishek Gupta, Somsree Roy, Vishwadeep Mane, Olivier Hamant, Utpal Nath

Recruitment of SH3P2–DRP1A by HYCCIN2 Drives Membrane Tubulation in Arabidopsis Embryonic Cell Plate Formation

Ya-Wen Hsu, Chine-Ta Juan, Cian-Ling Guo, Huei-Jing Wang, Guang-Yuh Jauh

The fate of pollen in two morphologically contrasting buzz-pollinated Solanum flowers

Christian Ary Vasquez-Castro, Elodie Morel, Bernardo Garcia-Simpson, Mario Vallejo-Marin

Floral Reversion based micropropagation of Day-Neutral Cannabis sativa L

Darya Sadat Tabatabaei, Rambod Abiri, A.M.P. Jones

Genomic imprinting in an early-diverging angiosperm reveals ancient mechanisms for seed initiation in flowering plants

Ana M. Florez-Rueda, Mathias Scharmann, Leonardo P. de Souza, Alisdair R. Fernie, Julien B. Bachelier, Duarte D. Figueiredo

Stable and dynamic gene expression patterns over diurnal and developmental timescales in Arabidopsis thaliana

Ethan J Redmond, James Ronald, Seth J Davis, Daphne Ezer

Integrated Transcriptome and Metabolome Analyses Reveal the Mechanism Regulating Bulbil Initiation and Development in Cystopteris chinensis

An Yu, Xiaohong Chen, Wenkai Xi, Xia Zhao, Yazhu Wang, Zhihong Gong, Xiaofeng Zhou

A genome-wide association screen for genes affecting leaf trichome development and epidermal metal accumulation in Arabidopsis

Radek Bezvoda, Yazmín Mónica Landeo-Ríos, Zdeňka Kubátová, Eva Kollárová, Ivan Kulich, Wolfgang Busch, Viktor Žárský, Fatima Cvrčková

From Bezvoda et al. This image is made available under a CC-BY 4.0 International license.

Integration of light quality signals regulates ABA abundance and stomatal movements during seedling establishment

Mathilda Gustavsson, Lionel Hill, Keara A. Franklin, Ashley J. Pridgeon

Non-CG DNA methylation modulates hypocotyl elongation during thermormorphogenesis

Maián Garro, Eleonora Greco, Gustavo J. Vannay, Aleksandra Leonova, Leonardo Bruno, Matías Capella

| Evo-devo

Climatic-environmental influences on hominin brain size over the last 5 million years

Samuel L. Nicholson, Thomas A. Püschel, Joanna Baker, Chris Venditti

Toxoplasma gondii infection misdirects placental trophoblast lineage specification

Leah F. Cabo, Liheng Yang, Mingze Gao, Rafaela J. da Silva, NyJaee N. Washington, Sarah M. Reilly, Christina J. Megli, Carolyn B. Coyne, Jon P. Boyle

Acute dietary methionine restriction highlights sensitivity of neocortex development to metabolic variations

Sulov Saha, Clemence Debacq, Christophe Audouard, Thomas Jungas, Pierrick Dupre, Mohamad Ali Fawal, Clement Chapat, Henri-Alexandre Michaud, Laurent Le Cam, Matthieu Lacroix, David Ohayon, Alice Davy

Direct measurement of the mutation rate and its evolutionary consequences in a critically endangered mollusk

T. Brock Wooldridge, Sarah Ford, Holland Conwell, John Hyde, Kelley Harris, Beth Shapiro

Regulatory networks of KRAB zinc finger genes and transposable elements changed during human brain evolution and disease

Yao-Chung Chen, Arnaud Maupas, Katja Nowick

A glyphosate-based herbicide selects for genetic changes while retaining within-species diversity in a freshwater bacterioplankton community

Emma Derrick, Naíla Barbosa da Costa, Rowan D. H. Barrett, B. Jesse Shapiro

Shared selection and genetic architecture drive strikingly repeatable evolution in long-term experimental hybrid populations

Gregory L. Owens, Celine Caseys, Nora Mitchell, Sariel Hübner, Kenneth D. Whitney, Loren H. Rieseberg

Conserved Evolutionary Response to Whole Genome Duplication in Angiosperms Revealed Using High Resolution Gene Expression Profiling

J. Luis Leal, Eva Hodková, Anja Billhardt, D. Magnus Eklund, Gustaf Granath, Pilar Herrera Egoavil, Jun Chen, Pascal Milesi, Jarkko Salojärvi, Martin Lascoux

Convergent evolution associated with the loss of developmental diapause may promote extended lifespan in bees

Priscila K. F. Santos, Karen M. Kapheim

How environment and genetic architecture of unreduced gametes shape the establishment of autopolyploids

Yu Cheng, Filip Kolář, Roswitha Schmickl, Josselin Clo

Cell differentiation controls iron assimilation in a choanoflagellate

Fredrick Leon, Jesus M. Espinoza-Esparza, Vicki Deng, Maxwell C. Coyle, Sarah Espinoza, David S. Booth

Morphometric evaluation of the anterior cranial fossa during the prenatal stage in humans and its clinical implications

Derkowski Wojciech, Kędzia Alicja, Dudek Krzysztof, Glonek Michał

Ventral-to-dorsal electrocyte development in electric organs of electric eel (Electrophorus)

Sinlapachai Senarat, Ayako Matsumoto, Shintaro Sakaki, Daichi Tsuzuki, Kazuko Uchida, Makoto Kuwahara, Eiichi Hondo, Atsuo Iida

An evolutionarily conserved Hox-Gbx segmentation code in the rice coral Montipora capitata

Shuonan He, Emma Rangel-Huerta, Eric Hill, Lacey Ellington, Shiyuan Chen, Sofia Robb, Eva Majerov, Crawford Drury, Matthew Gibson

Homeodomain protein codes specify neuronal diversity in the first ganglion of the Drosophila visual system

Chundi Xu, Peter Newstein, Rishi Sastry, Chris Doe

The multi-level effect of chlorpyrifos during clownfish metamorphosis

Mathieu Reynaud, Stefano Vianello, Shu-Hua Lee, Pauline Salis, Kai Wu, Bruno Frederich, David Lecchini, Laurence Besseau, Natacha Roux, Vincent Laudet

Evolution of the neural sex-determination system in insects: The doublesex homolog in crickets functions as an X-linked masculinizing factor to regulate morphological but not behavioral sexual dimorphism

Takayuki Watanabe

The role of vibration amplitude in the escape hatching response of red-eyed treefrog embryos

Julie Jung, Michael S. Caldwell, J. Gregory McDaniel, Karen M. Warkentin

Cell Biology

The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation and tissue organization in the C. elegans germ line

Réda M. Zellag, Vincent Poupart, Takefumi Negishi, Jean-Claude Labbé, Abigail R. Gerhold

From Zellag et al. This image is made available under a CC-BY-NC 4.0 International license.

Distinct Non-occluding Functions of Septate Junction Components in Signaling Pathway Regulation and Cell Polarity During Epithelial Development

V. Holtwick, A. Schubert, K. Rust

Non-random segregation of mitochondria during asymmetric cell division contributes to cell fate divergence in daughter cells

Ioannis Segos, Jens Van Eeckhoven, Simon Berger, Nikhil Mishra, Eric J. Lambie, Barbara Conradt

Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells

Seiichi Koike, Masashi Tachikawa, Motosuke Tsutsumi, Takuya Okada, Tomomi Nemoto, Kazuko Keino-Masu, Masayuki Masu

Optimized expansion microscopy reveals species-specific spindle microtubule organization in Xenopus egg extracts

Gabriel Guilloux, Maiko Kitaoka, Karel Mocaer, Claire Heichette, Laurence Duchesne, Rebecca Heald, Thierry Pécot, Romain Gibeaux

The oocyte zinc transporter Slc39a10/Zip10 is a regulator of zinc sparks during fertilization in mice.

Atsuko Kageyama, Narumi Ogonuki, Takuya Wakai, Takafumi Namiki, Yui Kawata, Manabu Ozawa, Yasuhiro Yamada, Toshiyuki Fukada, Atsuo Ogura, Rafael A. Fissore, Naomi Kashiwazaki, Junya Ito

gilgamesh, Drosophila casein kinase 1g, is required for myosin-dependent junction strengthening and epithelial folding

Lingkun Gu, Reina Benefiel, Jasneet Brar, Mo Weng

Decidual natural killer cells promote extravillous trophoblast developmental pathways: evidence from trophoblast organoid co-cultures

Morgan Zych, Natalie Lo, Kate A Patton, Kewei Wang, Brian J Cox

Distinct H3K9me3 heterochromatin maintenance dynamics govern different gene programs and repeats in pluripotent cells

Jingchao Zhang, Greg Donahue, Michael B. Gilbert, Tomer Lapidot, Dario Nicetto, Kenneth S. Zaret

Co-transcriptional RNA processing boosts zygotic gene activation

Jingzhao Xu, XiaoJing Li, Xiaowen Hao, Xinyun Hu, Shaoqian Ma, Yantao Hong, Jing Zhang, Dingfei Yan, Haiteng Deng, Jie Na, Xiong Ji, Zai Chang, Xiaohua Shen

“Mitotic” kinesin-5 is a dynamic brake for axonal growth

Wen Lu, Brad S. Lee, Helen Xue Ying Deng, Margot Lakonishok, Enrique Martin-Blanco, Vladimir I. Gelfand

Restorative macrophage-derived RNAseT2 stimulates muscle stem cell fusion via an SLK/N-WASP/actin bundling dependent axis

Michèle Weiss-Gayet, Gaëtan Juban, Emmeran Le Moal, Antonio Moretta, Camilla Farnetani, Christelle Gobet, Jules Guillemaud, Marie-Catherine Le Bihan, Oded Shoseyov, Annie Adrait, Katharina Ternka, Odile Boespflug-Tanguy, Matthias Kettwig, Yohann Couté, Rémi Mounier, Francesco Acquati, Robert D Knight, Bénédicte Chazaud

Key roles of the zona pellucida and perivitelline space in promoting gamete fusion and fast block to polyspermy inferred from the choreography of spermatozoa in mice oocytes

Yaělle Dubois, Sophie Favier, Nathan Martin-Fornier, Mohyeddine Omrane, David Stroebel, Eric Perez, Sandrine Barbaux, Ahmed Ziyyat, Nicolas Rodriguez, Christine Gourier

Pdgfab/Pdgfra-mediated chemoattraction guides the migration of sclerotome-derived fibroblast precursors in zebrafish

Emilio E. Méndez-Olivos, Katrinka M. Kocha, Shan Liao, Peng Huang

Localized translation of erm-1 contributes to ERM-1 function in the C. elegans embryo

Elise van der Salm, Esther Koelewijn, Erica van der Maas, Max Eeken, Suzan Ruijtenberg

Planar cell polarity coordination in a cnidarian embryo provides clues to animal body axis evolution

Julie Uveira, Antoine Donati, Marvin Léria, Marion Lechable, François Lahaye, Christine Vesque, Evelyn Houliston, Tsuyoshi Momose

From Uveira et al. This image is made available under a CC-BY-ND 4.0 International license.

Modelling

Cell geometry, microtubule anchoring and anisotropic dynamic instability: challenges and solutions to transverse cortical array organization

Tim Y.Y. Tian, Geoffrey O. Wasteneys, Colin B. Macdonald, Eric N. Cytrynbaum

From Genes to Patterns: A Framework for Modeling the Emergence of Embryonic Development from Transcriptional Regulation

Jimena Garcia-Guillen, Ezzat El-Sherif

Single-cell multi-omics, spatial transcriptomics and systematic perturbation decode circuitry of neural crest fate decisions

Zhiyuan Hu, Sarah Mayes, Weixu Wang, José M. Santos-Pereira, Fabian Theis, Tatjana Sauka-Spengler

Dynamic landscapes and statistical limits on growth during cell fate specification

Gautam Reddy

(Un)buckling mechanics of epithelial monolayers under compression

Chandraniva Guha Ray, Pierre A. Haas

Engineering morphogenesis of cell clusters with differentiable programming

Ramya Deshpande, Francesco Mottes, Ariana-Dalia Vlad, Michael P. Brenner, Alma dal Co

Modelling and calibration of pair-rule protein patterns in Drosophila embryo: From Even-skipped and Fushi-tarazu to Wingless expression networks

Catarina Dias, Rui Dilão

Tools & Resources

Ultrack: pushing the limits of cell tracking across biological scales

Jordão Bragantini, Ilan Theodoro, Xiang Zhao, Teun A.P.M. Huijben, Eduardo Hirata-Miyasaki, Shruthi VijayKumar, Akilandeswari Balasubramanian, Tiger Lao, Richa Agrawal, Sheng Xiao, Jan Lammerding, Shalin Mehta, Alexandre X. Falcão, Adrian Jacobo, Merlin Lange, Loïc A. Royer

From Bragantini et al. This image is made available under a CC-BY-NC 4.0 International license.

Disrupted developmental signaling induces novel transcriptional states

Aleena Patel, Vanessa Gonzalez, Triveni Menon, Stanislav Y. Shvartsman, Rebecca Burdine, Maria Avdeeva

Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets

Joost J.A.P.M. Wijnakker, Gijs J. F. van Son, Daniel Krueger, Willine van de Wetering, Carmen Lopez-Iglesias, Robin Schreurs, Fenna van Rijt, Sangho Lim, Lin Lin, Peter J. Peters, Ralph R. Isberg, Claudia Yanda, Wim de Lau, Hans Clevers

A simple immunohistochemical method for perinatal mammalian ovaries revealed different kinetics of oocyte apoptosis caused by DNA damage and asynapsis

Hiroshi Kogo, Akiko Iizuka-Kogo, Hanako Yamamoto, Maiko Ikezawa, Yukiko Tajika, Toshiyuki Matsuzaki

A community-oriented, data-driven resource to improve protocol design for cardiac modelling from human pluripotent stem cells

Chris Siu Yeung Chow, Sumedha Negi, Shaine Chenxin Bao, Chen Fang, James E. Hudson, Woo Jun Shim, Yuanzhao Cao, Nathan J. Palpant

Large-scale mouse mutagenesis identifies novel genes affecting vertebral development.

Ximena Ibarra-Soria, Elizabeth Webb, John Mulley

Mutant scaling laws reveal that accelerated evolution via gene amplification requires spatially structured population growth

Natalia L. Komarova, Justin Pritchard, Dominik Wodarz

Single-cell proteomics of pre-implantation mouse embryos uncovers distinct asymmetry of certain proteins among early blastomeres

Yuan Yuan, Mo Hu, Yinghui Zheng, Yutong Zhang, Yuxuan Pang, Xiaoliang Sunney Xie

Zebrafish glial-vascular interactions progressively expand over the course of brain development

Lewis G Gall, Courtney M Stains, Moises Freitas-Andrade, Bill Z Jia, Nishi Patel, Sean G Megason, Baptiste Lacoste, Natasha Meyer O’Brown

Transient Notch Activation Converts Pluripotent Stem Cell-Derived Cardiomyocytes Towards a Purkinje Fiber Fate

David M. Gonzalez, Rafael Dariolli, Julia Moyett, Stephanie Song, Bhavana Shewale, Jacqueline Bliley, Daniel Clarke, Avi Ma’ayan, Stacey Rentschler, Adam Feinberg, Eric Sobie, Nicole C. Dubois

Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells

Youjun Wu, Aaron Zhong, Alessandro Evangelisti, Mega Sidharta, Lorenz Studer, Ting Zhou

Quantitative phase imaging with temporal kinetics predicts hematopoietic stem cell diversity

Takao Yogo, Yuichiro Iwmaoto, Becker Hans Jiro, Takaharu Kimura, Ayano Sugiyama-Finnis, Tomomasa Yokomizo, Toshio Suda, Sadao Ota, Satoshi Yamazaki

Optimized expansion microscopy reveals species-specific spindle microtubule organization in Xenopus egg extracts

Gabriel Guilloux, Maiko Kitaoka, Karel Mocaer, Claire Heichette, Laurence Duchesne, Rebecca Heald, Thierry Pecot, Romain Gibeaux

A Drosophila holidic diet optimised for growth and development

Sebastian Sorge, Victor Girard, Lena Lampe, Vanessa Tixier, Alexandra Weaver, Theresa Higgins, Alex P. Gould

An explainable map of human gastruloid morphospace reveals gastrulation failure modes and predicts teratogens

Joseph Rufo, Chongxu Qiu, Dasol Han, Naomi Baxter, Gabrielle Daley, Maxwell Z. Wilson

Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

Çağrı Çevrim, Béryl Laplace-Builhé, Ko Sugawara, Maria Lorenza Rusciano, Nicolas Labert, Jacques Brocard, Alba Almazán, Michalis Averof

From Çevrim et al. This image is made available under a CC-BY 4.0 International license.

Research practice & education

From impact metrics and open science to communicating research: Journalists’ awareness of academic controversies

Alice Fleerackers, Laura L. Moorhead, Juan Pablo Alperin, Michelle Riedlinger, Lauren A. Maggio

When crayfish make news, headlines are correct but still misleading

Zen Faulkes

Updated science-wide author databases of standardized citation indicators including retraction data

John P. A. Ioannidis, Angelo Maria Pezzullo, Antonio Cristiano, Stefania Boccia, Jeroen Baas

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Catch up on Development presents… October webinar on environment, evolution and development

Posted by , on 7 October 2024

The 2 October 2024 Development presents… webinar was chaired by Development’s Guest Editor, Karen Sears (UCLA) and featured three talks on the topic of environment, evolution and development. Catch up on the talks below.

Catch up on previous webinars and register for new ones.

Sign up to the Development presents… mailing list to learn about the upcoming webinars as they are announced.

Girish Kale (University of Hohenheim)

Talk and Q&A

Natasha Shylo (Stowers Institute for Medical Research)

Talk and Q&A

Sergio Menchero (The Francis Crick Institute)

Talk and Q&A
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Apply to become a reporter at the Biologists @ 100 conference!

Posted by , on 3 October 2024

Are you keen to get more science communication experience? Is your research related to developmental and stem cell biology? The Node is looking for a reporter to attend and report from the Biologists @ 100 conference, happening 24-27 March 2025 in Liverpool, UK. This conference is a celebration of the 100th birthday of The Company of Biologists, bringing together different communities, including cell, developmental, experimental and disease biologists, and will incorporate the Spring Meetings of the British Society for Cell Biology (BSCB) and the British Society for Developmental Biology (BSDB). Registration for the conference is open and the abstract deadline is 13 December.

The selected reporter will have their conference registration fee waived (but will have to cover their own transport and accommodation costs*). They will be expected to attend the full conference. While the reporter will mainly focus on reporting from the ‘Cell and developmental biology’ scientific track, they are welcome to attend sessions from other tracks in the programme.

If you are interested, we’d love to read about your ideas of what you’d do as a reporter at the conference. If selected, we will work with you to develop your ideas and assist you at the conference.

If you have any questions about the role, don’t hesitate to email us at thenode@biologists.com.

To apply, please email the following to thenode@biologists.com:

  • A proposal (max 200 words) with idea(s) of what you would do as a conference reporter. We are open to suggestions of different formats of reporting. Please include a rough output you expect to produce from the reporting, and explain how the content will appeal to the Node readers.
  • A paragraph (max 200 words) telling us about yourself, your research, and any relevant science communication experiences you have.

Application deadline: Friday 29 November 2024

Our sister community site FocalPlane is also looking for a conference reporter. If you want to focus on reporting on cell biology and the use of microscopy in research, head over to their website to apply.

*Note: we encourage you to apply for conference grants to cover the transport/ accommodation costs, such as grants from BSDB and DMM.

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What’s new at InSDB

Posted by , on 1 October 2024

The Indian Society of Developmental Biologists is the national society that represents developmental and stem cell biologists across India. We are pleased to introduce the society and our activities to the Node readers. Reading on, you will find some of our recent initiatives and posts. Do visit the InSDB website to check out more. You can get in touch with us at info@insdb.in if you would like to contribute/be a volunteer for InSDB.

Gulmohar-Postdoc Talk Series

Gulmohar is our new, virtual, post-doc talk series through which we aim to bring together early-career scientists from around the world to share their research. This is a chance to hear from the next crop of PIs about their work, where their research is heading, and what’s next for them. Whether you are starting your research career, or already knee-deep in it, through this series, you will get to know about the different fields within the broad scope of developmental biology research. After every session, we also plan to have a post-talk informal discussion where you get to connect with the speakers and receive insight into their scientific journey and potential opportunities for your own.

We are currently accepting applications from postdocs who would like to be a speaker for the series. If you fit the bill, please sign up here, and we will get in touch with you.

Science Simplified

Science Simplified is our series where we pick up a recently published research paper in developmental biology and distill it to the liking of a general audience. You can read the first article in this series here

InSDB is also accepting articles from our audience! These could be stories on the latest research from the stem cell and developmental biology fields and perspectives on matters that are relevant to our audience. Anyone is welcome to pitch stories to us. We accept articles that are thoroughly researched and convey the excitement, relevance, and fascination of the subject to any curious reader. If you are interested in science writing and would like to sharpen your skills, this is your cue to get your pen and paper out! Also, do read our submission guidelines to know more. If you would like to contribute, please add your entries here.

Behind The Bench

Behind the Bench is our interview series where we feature InSDB members, their stories, and the science they do. In each episode, we sit down with a member to know about their journey in science, from their early inspirations to the serendipitous moments along the way. Through this series of interviews, we aim to highlight the members who make our society what it is today. You can read these interviews here.

If you would like to follow our updates closely, we are on X, LinkedIn, and Instagram!

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Counterpoint in Science: Power of Tandem talks at the Interfaces between Physics and Biology

Posted by , on 26 September 2024

HFSPUnistra Symposium available online featuring Eric Wieschaus and William Bialek, Anthony Hyman and Frank Jülicher, Donatas Zigmantas and Thomas Renger, musical duo from the Philharmonic Orchestra of Strasbourg and more

Daniel Riveline,1 Karsten Kruse,2 Mukund Thattai,3 Guntram Bauer4

Scientific communication was pioneered in the 17th century (Ref. 1). Scientists were called ‘sçavans’ and had to embrace the humanities and the sciences to present their discoveries with clarity. These achievements are difficult nowadays, with the detailed technical knowledge involved in biology, physics, chemistry and mathematics research. In this context, it is challenging to present the depth of each side of an interdisciplinary scientific question, and often one field is featured more prominently, to the detriment of the other. To address this issue, we organised a Symposium in which presentations were given as tandem talks, with participants from biology and physics or from chemistry and biology. Topics ranged from cell positioning in the embryo (Eric Wieschaus and William Bialek), to liquid condensates (Anthony Hyman and Frank Jülicher), to the physics of photosynthesis (Donatas Zigmantas and Thomas Renger). We found this tandem format to be extremely successful (see Symposium online Ref. 2). Concepts from physics, biology and chemistry were explained in accessible yet informative language, and the audience could see how interdisciplinary engagements developed. Importantly, the speakers highlighted active points of debate and articulated unresolved issues, leaving space for new breakthroughs and scientific directions. As a metaphor for collaboration, a musical duo on the violin and cello illustrated how each voice in a partnership could be heard. Using a Bach counterpoint as the paradigm, the musicians from the Philharmonic Orchestra of Strasbourg explained how their pieces were harmonious only with shared and alternating voices.

References:

1- A History of Scientific Journals, Aileen Fyfe, Noah Moxham, Julie McDougall-Waters, Camilla Mørk Røstvik, UCL Press London (2022) https://www.uclpress.co.uk/products/187262

2- Frontier Science at the Interface of Physics and Biology 2024, HFSP-Unistra Symposium online https://www.canalc2.tv/video/16553

Affiliations:

1- Laboratory of Cell Physics, IGBMC, CNRS and University of Strasbourg, Strasbourg, France, riveline@unistra.fr

2- Departments of Biochemistry and Theoretical Physics, University of Geneva, 1211 Geneva, Switzerland, Karsten.Kruse@unige.ch

3- Simons Centre for the Study of Living Machines, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru, India, thattai@ncbs.res.in

4- The International Human Frontier Science Program Organization, 12 Quai Saint-Jean, 67080 Strasbourg, France, gbauer@hfsp.org

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