The International Society for Regenerative Biology, founded in 2020, to promote community, research, and education in the field of regeneration worldwide. Its core mission is to provide new opportunities for interactions, discoveries, and recognition for scientists at all stages who are interested in regenerative biology.
ISRB is now offering a webinar series with three webinars a month based in three different time zones around the world.
Zone 1: East and South Asia, webinar will be the first week of the month.
Zone 2: Europe, Africa, webinar will be the second week of the month.
Zone 3: the Americas, webinar will be the third week of the month.
More information on exact dates and times can be found on our website. We hope you will join us!
Mel White, a Principal Investigator and Lakshmi Balasubramaniam, a Postdoctoral Researcher, both attendees of EGGED 2022, provide accounts of their experiences at the workshop in July.
Dr Mel White on left and Dr Lakshmi Balasubramaniam on right
Dr Mel White is a Group Leader and ARC Future Fellow, based at The University of Queensland, Australia.
As a new PI who started my lab in Australia during the COVID-19 pandemic, I was very excited to attend the inaugural EGGED 2022 workshop in Edinburgh, Scotland. Hosted by the Roslin Institute, The Royal (Dick) School of Veterinary Studies & The Roslin Institute’s National Avian Research Facility (NARF). The Edinburgh Gallus Genomics and Embryonic Development Workshop (EGGED) brought together scientists working with or interested in using the chicken embryo as a research model. By combining seminars from invited speakers and practical hands-on demonstrations in the lab, EGGED 2022 showcased the power and versatility of the avian embryo.
The workshop opened with some background about NARF and the exciting transgenic chicken lines already created and in progress. For those new to the avian model, there was an introduction to the chicken embryo and its unique advantages for addressing questions of a spatiotemporal nature by renowned embryologist Claudio Stern. Over four days, further seminars highlighted an impressive range of avian research including teratology (Neil Vargesson), development of the eye (Joe Rainger), limb (Julia Oh) and neural tube (Ashley Libby), neuronal differentiation (Raman Das), determining gene regulatory networks (Ruth Williams, Tatjana Sauka-Spengler), development across scales (Ben Steventon), mechanobiology (Jérôme Gros) and recent advances in our understanding of the chicken genome (Jacqueline Smith, Hervé Acloque). Each day we also spent time in the Vet School teaching lab watching practical demonstrations and practising fundamental techniques such as embryo staging, ex ovo culturing, electroporation, grafting, bead application and organ slice culture. Additionally, there was a full day available to try personalised experiments using our new skills and the NARF transgenic lines.
The generosity of everyone involved in EGGED was truly amazing. As organisers, Megan Davey, Lindsay Henderson and their team worked tirelessly behind the scenes incubating thousands of eggs, setting up equipment and juggling logistics for all manner of experiments. A special mention goes to the indispensable Julia Oh who presented or demonstrated every day, cheerfully sharing her knowledge and answering endless questions. Many of the invited speakers also spent hours sharing their expertise in the labs and discussing science with us long into the evening at the social events afterwards. The attendees ranged from students to PIs, with all levels of avian experience and it was inspiring to see the free exchange of ideas and knowledge as people passed on tips and tricks and shared their specialist skills.
But perhaps even more important than the knowledge we gained were the connections we made. Thanks to the explicit intentions of the organisers, EGGED 2022 was a collaborative workshop where we not only came to learn, but also to join as a community to shape the future of avian research. Over a buffet dinner and drinks, we discussed ideas to enhance access and utility of the model and cooperation and exchange between avian researchers. After a traditional Scottish send-off of boisterous Ceilidh dancing, I returned home to Australia bubbling with ideas and excited to be part of this welcoming research community.
Group picture of EGGED 2022 participants, speakers and organisers outside of the National Avian Research Facility.
Dr Lakshmi Balasubramaniam, is an EMBO Postdoctoral Fellow in the Xiong Lab, based at The Gurdon Institute, University of Cambridge, UK.
Contrary to the name of this workshop, the aim wasn’t to be egged but explore the different techniques and build networks among those using avian embryo as a model system to study various biological questions ranging from transgenics, developmental biologists to viral production. The EGGED workshop gathered established researchers, early career researchers (postdocs, young PIs), and PhD students from varying stages of their career to discuss and share their knowledge, techniques and tips to get experiments working from the ground up. Expertise of participants varied from newbies (<1 month of experience) to those who have been working with avian embryos for several years. This was definitely a platform to accelerate one’s progress and discuss ideas with a group of likeminded individuals where problems were shared over meals while coming up with solutions during the practical lab sessions.
This 4 day workshop, was packed with networking, lab-based practical work and talks by invited speakers. With a participation of about 40 researchers, the first day started with an introduction to the workshop by organisers Megan Davey and Lindsay Henderson where they introduced the Roslin Institute, the transgenic facility and the home office regulations in place to regulate animal work in the UK. This was followed by a short introduction to the staging of chicken embryos and early development of chicken embryos by Claudio Stern. The afternoon was filled with lab-based practical sessions where we were introduced to various ways of embryo isolation, staging them and identifying the viability of embryos. This was followed by a practical session led by Neil Vargesson where he gave us an introduction to the use of beads to coat proteins/drugs in localized regions followed by free form lab work.
The second day started off with a talk by Mike McGrew who introduced his latest technique accelerating transgenic development by growing PGC’s in-vitro. Following this we had an overview of various early career researchers demonstrating the use of transgenics in different contexts ranging from eye development, limb regeneration and neural tube development. We then nosedived into a practical session led by Megan Davey and Julia Oh who demonstrated tissue grafting to trace tissue integration using transgenic embryos. While many of us failed to have embryos that survived overnight, a handful of embryos survived overnight culture demonstrating the power of this technique. Following this we had a session led by Raman Das who showed us explant cultures enabling us to live image inaccessible regions of the embryo at high resolution. The last session of the day had Ruth Williams demonstrating in-ovo and ex-ovo electroporation as a tool to genetically engineer chicken embryos. Many of us had the opportunity to test out both of these setups and get tips from Ruth on how they could be adapted for use in our labs.
Image from Mel on left, a windowed egg with an early-stage embryo. From Lakshmi on right, a GFP transgenic chicken embryo at approx. stage 26HH, under a fluorescence microscope.
The third day started off with a talk by Ben Steventon where he introduced the role of the node, which was followed by a free form lab session where we were left to play with the techniques we learnt. In parallel we were shown how to make tools, ex-ovo imaging of chicken embryos in a cup and tips on gene editing, CRISPR construct design, and PGC injections. This was followed by a session on clearing techniques and an introduction to Zeiss lightsheet. The day ended with talks by Jacqueline Smith who discussed the role of using avian embryos to study viral host interaction while Hervé Acloque introduced us to his initiatives to annotate avian genes, the FAANG initiative, Galdbase (genomics and sequencing data of avian embryos) and the VizFaDa (tool to visualize sequencing datasets). While the day of practical work and talks ended there, scientific discussions continued over at the “Brainstorming Buffet” where participants continued to discuss publishing, funding, ethics, science communication and a myriad of new ideas in small groups that swapped around the room.
The last day of the workshop started off with online talks by Tatjana Sauka-Spengler who discussed their work on generating single cell atlas during neurulation, and transcriptional activity during neurulation and neural tube closure. This was followed by Jérôme Gros who shared their latest work showing gastrulation to be mediated by signalling molecules like GDF1 with feedback from mechanics. He also described their efforts to develop various transgenic quail lines that are available for the wider community. Before continuing our last day of practical sessions, we were introduced to HCR as an alternative to RNA hybridization. We were walked through the different steps during HCR, with tips and how one goes about ordering and designing HCR probes. We were generously gifted with starter kits by Molecular Instruments kind sponsors of this workshop. The workshop ended on a high note with dinner that had us put on our dancing shoes to dance to Ceilidh followed by goodbyes at the end of this 4 day workshop, which was packed with information, tips and networking. To me, this was an opportunity to learn the different techniques used to study avian embryogenesis while building a network of researchers working on similar questions.
Had such a great time at #egged2022 this past week! Met so many talented researchers and learnt about some really cool techniques! Really excited to see where this all goes! Thank you so much @twobluechickens, @GlanceScience and all funders! pic.twitter.com/i2adcJFaJ4
Please join us for the next exciting ISRB webinar featuring Erin Davies, Ph.D., of the National Cancer Institute, USA and Samantha Joyce Hack, of Western Michigan University, USA. The Zone 3 talk will occur September 20 at 12pm East Coast Time. Theme: Planarian Regeneration.
Erin’s talk is titled “Embryonic origins of lifelong regenerative abilities“ Samantha’s talk is titled “Planar cell polarity signaling is required to terminate regenerative growth in the planarian Schmidtea mediterranea”
There will be time after each talk for live questions. For those who are unable to join live, we will post a link to the recording of the webinar in the #isrb_webinars channel of the ISRB slack. The recording will be available for 48 hours. The speakers have agreed to closely monitor that channel for those 48 hours to answer questions that you might have.
We will be holding these webinars monthly and will be alternating between time zones. Each month you should pay close attention to the time, because this will change. Each month we will choose two speakers around a new theme from similar time zones. The talk will take place around 4pm of the time zone the speakers comes from.
Please click here to add the ISRB calendar to your Google calendars. Upcoming webinars will be posted here. We will continue to send out email reminders and Tweet with speaker and Zoom details.
Please join us for the next exciting ISRB webinar featuring Chiara Sinigaglia, PhD of the Oceanographic Observatory of Banyuls-sur-Mer Sorbonne University/CNRS, France. And, Simon Blanched, PhD, Department of Biology, University of Fribourg, Switzerland. This month’s theme is invertebrate regeneration. The Zone 2 talk will occur September 14 at 4pm Central European Time.
Chiara’s talk is titled: To regenerate or not to regenerate? Chemico-mechanical regulation of body shape in wounded jellyfish. Simon’s talk is titled: Whole-body regeneration in Botrylloides diegensis.
There will be time after each talk for live questions. For those who are unable to join live, we will post a link to the recording of the webinar in the #isrb_webinars channel of the ISRB slack. The recording will be available for 48 hours. The speakers have agreed to closely monitor that channel for those 48 hours to answer questions that you might have.
We will be holding these webinars monthly and will be alternating between time zones. Each month you should pay close attention to the time, because this will change. Each month we will choose two speakers around a new theme from similar time zones. The talk will take place around 4pm of the time zone the speakers comes from.
Please click here to add the ISRB calendar to your Google calendars. Upcoming webinars will be posted here. We will continue to send out email reminders and Tweet with speaker and Zoom details.
Applications are invited for a Postdoctoral Research Scientist to join A/Prof Duncan Sparrow’s laboratory at the University of Oxford to work on a project using mouse models to investigate environmental influences on embryonic heart development that may lead to congenital heart disease in humans. This is a 5 year position funded by the British Heart Foundation. Applications are particularly welcome from women, black and minority ethnic candidates who are under-represented in academic posts in Oxford. tinyurl.com/2s387496
With the start of the academic year, we are continuing to dive into our archives to remind you of some the fantastic content that we hope will help you have a great year! This week we focus on writing, and we’ll start with a reminder that one of our favourite quotes from John Wallingford, the creator of the #devbiolwriteclub.
“Writing is like a sport. You only get good at it if you practice, with intent, every day.”
You can find John’s series of posts from the #devbiolwriteclub here, including a mention of his spin-off #devbiolgrantclub
We also have Grant writing advice for PhD students and Postdocs from Elisa Genie
For tips and tricks on concise science writing, our Reviews Editor Alex Eve shares his #wordcountchop article, while, our Senior Editor Seema Grewal takes us through the process of writing review articles from planning to the finished article.
If you are interested in practising your writing skills, we always welcome new authors on the Node. Please get in contact with us at thenode@biologists.com if you would like to discuss your ideas, or to get help with planning or editing. You can also get involved in science communication on our sister sites preLights and FocalPlane. preLights is where ECRs highlight preprints of their choice – you can find out how and why you should get involved here. Whilst FocalPlane is focussed on all things microscopy including ‘How to’ posts and blog series, among many other types of content that you can contribute to. You can find out how to get involved here.
“God forbid we find ourselves in a serious geopolitical conflict because of biology and technology merging in ways that people didn’t expect or anticipate”
Amy Webb
In the latest episode of the Genetics Unzipped podcast, we’re exploring the frontiers of genetic engineering and synthetic biology with Amy Webb and Andrew Hessel, authors of the new book The Genesis Machine – taking a look at what’s possible now, what’s coming fast in the future, and what we as individuals and wider society should do about it.
The Society for Developmental Biology Ethel Browne Harvey Postdoctoral Seminar Series is back on Friday, September 9, at 3 pm ET (9pm CEST). This seminar series was established in 2020 as a product of SDB’s Strategic Plan to holistically address the needs of members spanning diverse career stages and career paths. It highlights the work of SDB postdoc members, providing a platform for those going on the job market.
The series is named after Ethel Browne Harvey, a pioneering U.S. embryologist best known for her work on cell cleavage in sea urchins. But it was in 1909, during her time as a trainee with Edmund Beecher Wilson, that Browne Harvey performed one of the key experiments of her career when she transplanted the hypostome of one hydra into another, inducing a secondary axis in the host. Ultimately, these experiments support the idea of the organizer-effect, which was first described by Hans Spemann and Hilde Mangold in 1924. Through her career, Browne Harvey received many fellowships aimed at supporting women in science and based her research programme at the Marine Biological Laboratory in Woods Hole, as well as holding teaching positions at various schools and universities including Princeton.
Natalia Bottasso Arias from Cincinnati Children’s Hospital and Jeremy Sandler from the Stowers Institute for Medical Research will present in the first seminar of the season.
Natalia Bottasso Arias did her undergraduate studies in biochemistry at the University of La Plata in Argentina. She went on to earn her doctorate in cell biology and biophysics at the University of La Plata where she studied the role of fatty acid binding proteins in human disease under the mentorship of Dr. Betina Corsico and Dr. Fernando Chirdo. In 2019, Natalia joined Dr. Debora Sinner’s lab at Cincinnati Children’s Hospital Medical Center as a postdoctoral fellow. Her research focuses on understanding the molecular basis of large airway malformations and the specific roles of Wnt, Bmp and HH signaling in determining the cartilaginous and muscle lineage of the trachea. She is the recipient of the Arnold W. Strauss Fellow Award at Cincinnati Children’s.
Jeremy Sandler did his undergraduate studies at the University of Washington earning a B.S. in biology with minors in microbiology and international studies. He then worked as a researcher at the Lawrence Berkeley National Laboratory where he earned the SPOT Award for his “instrumental role in establishing next-generation sequencing technologies.” In 2010, Jeremy entered graduate school at the California Institute of Technology. He earned a Ph.D. in biology and biological engineering in the lab of Dr. Angela Stathopoulos where he studied genome activation and regulation of signaling in the rapidly dividing Drosophila embryo. In 2018, Jeremy joined Tatjana Piotrowski’s lab at the Stowers Institute for Medical Research where he studies mechanosensory hair cell regeneration in the zebrafish. He is a recipient of the NIH Ruth L. Kirschstein National Research Service Award.
Synthetic embryos have dominated the discussion on #devbio Twitter over the past few weeks, following ground-breaking publications from the Hanna and Zernicka-Goetz labs. The labs have produced synthetic mouse embryos with astonishing similarity to the ‘real thing’, which has prompted discussions on where this technology can go next, the distinction between embryo-like models and real embryos, ethical implications and responsible reporting to the general public. With fantastic timing, next Monday (12 September) at 16.00 BST, we will be livestreaming our panel discussion on technical, ethical and legal challenges of studying early human development from our Journal meeting on human development, where we expect to cover some of the topics discussed on Twitter in more depth. The stream of our panel discussion, as well as talks from Sarah Teichmann and Sergiu Pasca, will be hosted on the Node. For now, we have picked out a few of our favourite Tweets and replies for you to read (to check out the discussion, simple click on the tweets below).
– they do not have organismal potential – there's lots of work to do in the mouse system to boost efficiency (now 0.1 – 2%), which should imo be prioritized before going human
We are of course excited about our embryo-like model but wish to stress that it is important not to think of our embryo-model as being a real embryo – even if it is getting close to the real thing
Half-baked idea alert: if people are gonna take in vitro wombs to their logical conclusion, they should start with marsupials! Less important imprint regulation, and later implantation. And you only need to get this far. MAKE A KOALA DAMMIT pic.twitter.com/AJrg6uXck7
If you’ve seen something that we should include in our ‘Developing news’ report or you would like to help us put the blog together, please get in touch at thenode@biologists.com
Metabolic enhancement of mammalian developmental pausing Vera A. van der Weijden, Maximilian Stoetzel, Beatrix Fauler, Dhanur P. Iyer, Mohammed Shahraz, David Meierhofer, Steffen Rulands, Theodore Alexandrov, Thorsten Mielke, Aydan Bulut-Karslioglu
wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome Claire J. Watson, W. Joyce Tang, Maria F. Rojas, Imke A.K. Fiedler, Ernesto Morfin Montes de Oca, Andrea R. Cronrath, Lulu K. Callies, Avery Angell Swearer, Ali R. Ahmed, Visali Sethuraman, Sumaya Addish, Gist H. Farr III, Arianna E. Gomez, Jyoti Rai, Adrian T. Monstad-Rios, Edith M. Gardiner, David Karasik, Lisa Maves, Bjorn Busse, Yi-Hsiang Hsu, Ronald Young Kwon
β1 integrin regulates alveolar epithelial cell differentiation following injury Jennifer M.S. Sucre, Fabian Bock, Nicholas M. Negretti, John T. Benjamin, Peter M. Gulleman, Xinyu Dong, Kimberly T. Ferguson, Christopher S. Jetter, Wei Han, Yang Liu, Seunghyi Kook, Jason Gokey, Susan H. Guttentag, Jonathan A. Kropski, Timothy S. Blackwell, Roy Zent, Erin J. Plosa
Single cell, whole embryo phenotyping of pleiotropic disorders of mammalian development Xingfan Huang, Jana Henck, Chengxiang Qiu, Varun K. A. Sreenivasan, Saranya Balachandran, Rose Behncke, Wing-Lee Chan, Alexandra Despang, Diane E. Dickel, Natja Haag, Rene Hägerling, Nils Hansmeier, Friederike Hennig, Cooper Marshall, Sudha Rajderkar, Alessa Ringel, Michael Robson, Lauren Saunders, Sanjay R. Srivatsan, Sascha Ulferts, Lars Wittler, Yiwen Zhu, Vera M. Kalscheuer, Daniel Ibrahim, Ingo Kurth, Uwe Kornak, David R. Beier, Axel Visel, Len A. Pennacchio, Cole Trapnell, Junyue Cao, Jay Shendure, Malte Spielmann
Multi-organ functions of yolk sac during human early development Rachel A Botting, Issac Goh, Antony Rose, Simone Webb, Justin Engelbert, Yorick Gitton, Emily Stephenson, Mariana Quiroga Londoño, Michael Mather, Nicole Mende, Ivan Imaz-Rosshandler, Dave Horsfall, Daniela Basurto-Lozada, Nana-Jane Chipampe, Victoria Rook, Pavel Mazin, MS Vijayabaskar, Rebecca Hannah, Laure Gambardella, Kile Green, Stephane Ballereau, Megumi Inoue, Liz Tuck, Valentina Lorenzi, Kwasi Kwakwa, Clara Alsinet, Bayanne Olabi, Mohi Miah, Chloe Admane, Dorin-Mirel Popescu, Meghan Acres, David Dixon, Rowen Coulthard, Steven Lisgo, Deborah J Henderson, Emma Dann, Chenqu Suo, Sarah J Kinston, Jong-eun Park, Krzysztof Polanski, Stijn Van Dongen, Kerstin B Meyer, Marella de Bruijn, James Palis, Sam Behjati, Elisa Laurenti, Nicola K Wilson, Roser Vento-Tormo, Alain Chédotal, Omer Bayraktar, Irene Roberts, Laura Jardine, Berthold Göttgens, Sarah A Teichmann, Muzlifah Haniffa
Tissue-Like 3D Standard and Protocols for Microscope Quality Management Benjamin Abrams, Thomas Pengo, Rebecca C. Deagle, Nelly Vuillemin, Tse-Luen Wee, Linda M. Callahan, Megan A. Smith, Kristopher E. Kubow, Anne-Marie Girard, Joshua Z. Rappoport, Carol J. Bayles, Lisa A. Cameron, Richard Cole, Claire M. Brown
Junior scientists spotlight social bonds in seminars for diversity, equity, and inclusion in STEM Evan A. Boyle, Gabriela Goldberg, Jonathan C. Schmok, Jillybeth Burgado, Fabiana Izidro Layng, Hannah A. Grunwald, Kylie M. Balotin, Michael S. Cuoco, Keng-Chi Chang, Gertrude Ecklu-Mensah, Aleena K. S. Arakaki, Noorsher Ahmed, Ximena Garcia Arceo, Pratibha Jagannatha, Jonathan Pekar, Mallika Iyer, DASL Alliance, Gene W. Yeo
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