The Novo Nordisk Foundation Center for Stem Cell Biology (DanStem) addresses basic research questions in stem cell and developmental biology and has activities focused on the translation of promising basic research results into new strategies and targets for the development of new therapies for cancer and chronic diseases such as diabetes and liver failure. Learn more about DanStem at https://danstem.ku.dk/.
The Novo Nordisk Foundation Center for Protein Research (CPR) promotes basic and applied research on proteins of medical relevance. The research scope spans in silico biology, proteomics, biochemistry and cell biology, and the unifying theme is the identification and functional exploration of proteins and protein pathways involved in disease mechanisms. Learn more about CPR at www.cpr.ku.dk.
Both DanStem and CPR are vibrant, internationally diverse, ambitious research centers, housing modern laboratories with state-of-the-art facilities located in close proximity to one another at the Faculty of Health and Medical Sciences, University of Copenhagen. The setting is ideally suited for seamless exchange of expertise, material and lively scientific communication.
Advanced light microscopy is a key technology at DanStem and CPR. The Centers host approximately 15 research groups with over 50 users (Master and PhD students, Postdocs, Assistant and Associate Professors). Applications in cellular imaging include a variety of model systems (cell lines, 3D cell cultures, tissues, and organisms) and microscopy techniques (wide-field, confocal, high-throughput, light-sheet, FCS, FRAP, live-cell imaging). User support for microscopy and image analysis is provided by dedicated technology platforms at the Centers.
We are now seeking a highly motivated and qualified image analysis specialist to work within the Imaging Research Platform at DanStem and the Protein Imaging Facility within CPR, alongside the light microscopy specialists at both platforms.
Responsibilities
The image analysis specialist works in a team with dedicated microscopy specialists to provide service and support for multiple research projects of broad biomedical scope throughout the two research centers. The image analysis specialist at CPR and DanStem is expected to
Provide support and training for users of both Centers on a variety of image analysis software packages (e.g. Fiji/Image J, Bitplane/Imaris, Perkin Elmer/Harmony, Olympus/ScanR Analysis, Zeiss ZEN, Huygens)
Provide guidance in identifying optimal software and image analysis solutions for a given task
Help users with analysis and graphic presentation of multidimensional data (e.g. Tibco Spotfire)
Organize and participate in image analysis workshops (including practical demonstrations) for graduate and undergraduate students
Manage computer workstations for offline image analysis and take care of software purchases, installation, maintenance and updates (local IT-support is provided)
Establish a fully virtualized analysis environment for the above-mentioned analysis packages using our newly established HPC server (incl. dedicated GPU processing)
Initiative for and collaborations utilizing project-oriented coding of new image analysis tools using our HPC platform for high-content or light-sheet applications is welcome and will be supported.
Network actively with the global image analysis community and stay up-to-date with current trends in the field
Qualifications and competences
Candidates are expected to have a Master’s or PhD degree in a biological sciences-related discipline with at least three years of experience in bio-image analysis. Previous experience with user support in a facility environment is of advantage. Alternatively, candidates with relevant expertise, such as in computer science and data analysis or physics, coupled with a strong interest in cellular imaging and experience in working with biologists can be considered. In addition, we wish for the candidate to have:
A broad overview of image analysis tools and expertise with a variety of image analysis software packages
Experience with 2D, 3D and 4D image analysis and segmentation of structures
Experience with downstream data handling and analysis, including statistical evaluation
Expertise in development of custom image analysis solutions with Matlab, Fiji/ImageJ, Python, Java, R or similar programming languages
A creative mind as well as an active network to tackle challenging tasks in image analysis
Ideally, a good level of practical experience with advanced light microscopy, including wide-field, confocal and high-content microscopy
Excellent communication skills and fluency in English
A service-minded and team player attitude
Ability to work in a multidisciplinary and international environment
Employment conditions
The position, with formal employment title of special consultant at the University of Copenhagen, will be in accordance with the provisions of the collective agreement between the Danish Government and AC (the Danish Confederation of Professional Associations). To the salary is added a monthly contribution to a pension fund according to the collective agreement, and a supplement could be negotiated, depending on the candidate’s experiences and qualification. In all cases, the ability to perform the job will be the primary consideration, and thus we encourage all, regardless of their personal background and status, to apply.
Informal inquiries can be directed to Malte Paulsen (Head of Research Platforms at DanStem, malte.paulsen@sund.ku.dk) and Claudia Lukas (Head of Protein Imaging Facility, CPR, claudia.lukas@cpr.ku.dk).
To apply
To apply, please submit your application, in English, via https://employment.ku.dk/administrative/ by clicking “Apply online”. Applications must include a statement of motivation, curriculum vitae, and copies of relevant diplomas. Only applications in English and submitted online and be accepted.
The Centers highly value diversity and encourage applications from people of all backgrounds.
The closing date for applications is 28 February 2021.
Part of the International Alliance of Research Universities (IARU), and among Europe’s top-ranking universities, the University of Copenhagen promotes research and teaching of the highest international standard. Rich in tradition and modern in outlook, the University gives students and staff the opportunity to cultivate their talent in an ambitious and informal environment. An effective organisation – with good working conditions and a collaborative work culture – creates the ideal framework for a successful academic career.
We are looking for a highly motivated PhD student interested in zebrafish brain development, RNA metabolism, gene editing and transcriptomic analyses to join the Nikolaou Lab at the University of Bath. The deadline for applications is 31st January 2021. The anticipated start date for this project is early October 2021. Applicants should hold, or expect to receive, a First Class or good Upper Second-Class Honours degree (or the equivalent). A master’s level qualification would also be advantageous. UK and EU candidates (with settled or pre-settled status in the UK) applying for this project will be considered for a University Research Studentship which will cover UK/EU tuition fees, a training support grant of £1,000 per annum and a tax-free maintenance allowance at the UKRI Doctoral Stipend rate (£15,285 in 2020-21) for a period of up to 3.5 years.
Brief description of the project:
Regulation of pre-mRNA splicing plays a significant role in neurons by diversifying the proteome and modulating gene expression during development and in response to physiological cues. Although most pre-mRNA processing reactions are thought to occur in the nucleus, numerous RNA splicing regulators are also found in neurites, however, very little is known about their extra-nuclear functions. Our recent work showed for the first time that the non-nuclear pool of a major spliceosome component (SNRNP70) modulates the production of alternative spliced mRNA isoforms essential for motor connectivity and protects transcripts from degradation.
This project aims to investigate further the extra-nuclear activities of SNRNP70 in the context of neuronal connectivity in zebrafish. The ease of genetic manipulations together with the translucency and small size of their offspring allows us to monitor neural cell behaviour and function and observe changes in neuronal connectivity. We will use a range of genetic tools, including transgenic over-expression of cytoplasmic SNRNP70 and nuclear-only SNRNP70 zebrafish knock-in lines to establish developmental functions attributed to the cytoplasmic pool of SNRNP70. The results from this project will contribute to our understanding of how local RNA metabolism in axons contributes to the normal development of neural connections in the brain.
The International C. elegans Conference takes place every two years and features cutting-edge research in a diverse array of topics, including physiology, neurobiology, development, evolution, behavior, aging, ecology, gene regulation, genomics, and more. For 2021, #Worm21 has been reimagined for a virtual platform and will focus on early career researchers at every stage.
We’re happy to confirm the next webinar in our Development presents… series will be chaired by our Editor Swathi Arur (The University of Texas MD Anderson Cancer Center), who has brought together talks that span her interests in C. elegans development, the germline and cell signalling.
The webinar will be held in Remo, our browser-based conferencing platform – after the talks you’ll have the chance to meet the speakers and other participants at virtual conference tables. If you can’t make it on the day, talks will be available to watch for a couple of weeks after the event (look out for details on the Node).
For more information about what to expect in Remo, go to
Updated 11 January. Let us know if we missed anything
Various organisations and looser assemblies of locked down researchers have begun to put together online seminar and talk series, many of which are open to anyone (usually with registration), and many of which also have previous talks recorded.
Here’s a list of what we’ve found recently, developmental biology and adjacent – please let us know if we missed anything so we can keep it up to date. For upcoming virtual developmental biology conferences/symposia, see our Events calendar page.
First up from us is Development presents…, the webinar series hosted each month by a different Development Editor which will be a platform for early career researchers to share their work. As well as the talks, you also have the chance to meet the speakers and other participants at interactive video tables – giving the developmental biology community the chance to network virtually.
The next webinar will be Wednesday 13 January, 16.00 GMT, hosted by Swathi Arur and featuring talks from Brandon Scott Carpenter, Hayao Ohno and Swann Floc’hlay.
Next talk: January 14, Evolutionary Studies:
Just Under the Surface: Leveraging Zebrafish to Understand the Interplay Between Evolution and Development
We currently have an opening for a Reviews Editor as a maternity cover position on Development. As this is a temporary position, we are specifically looking for candidates with editorial experience.
Core responsibilities of the position include:
Commissioning, handling peer review and developmental editing of material for the front section of the journal
Representing the journal at international conferences and within the wider scientific community
Writing press releases, article highlights and material for Development’s community website ‘the Node’
Creative involvement in the journal’s development
For further details and instructions on how to apply, please see the full job advert here. If you are interested in applying, but would like further information or have any questions, please feel free to drop me an email.
Naa12 rescues embryonic lethality in Naa10-Deficient Mice in the amino-terminal acetylation pathway
Hyae Yon Kweon, Mi-Ni Lee, Max Dörfel, Seungwoon Seo, Leah Gottlieb, Thomas Papazyan, Nina McTiernan, Rasmus Ree, Andrew Garcia, Michael Flory, Jonathan Crain, Alison Sebold, Scott Lyons, Ahmed Ismail, Elaine Marchi, Seong-keun Sonn, Se-Jin Jeong, Sejin Jeon, Shinyeong Ju, Simon J. Conway, TaeSoo Kim, Hyun-Seok Kim, Cheolju Lee, Tae-Young Roh, Thomas Arnesen, Ronen Marmorstein, Gholson J. Lyon, Goo Taeg Oh
Serine Palmitoyltransferase Controls Stemness of Intestinal Progenitors
Ying Li, Bhagirath Chaurasia, Vincent Kaddai, Joseph L. Wilkerson, J. Alan Maschek, James Cox, Peng Wei, Claire Bensard, Peter J Meikle, Hans Clevers, James A Shayman, Yoshio Hirabayashi, William L. Holland, Jared Rutter, Scott A. Summers
CTCF is a Barrier for Totipotent-like Reprogramming
Teresa Olbrich, Maria Vega-Sendino, Desiree Tillo, Wei Wu, Nicholas Zolnerowich, Andy D. Tran, Catherine N. Domingo, Mariajose Franco, Marta Markiewicz-Potoczny, Gianluca Pegoraro, Peter C. FitzGerald, Michael J. Kruhlak, Eros Lazzerini-Denchi, Elphege P. Nora, Andre Nussenzweig, Sergio Ruiz
Robust integrated intracellular organization of the human iPS cell: where, how much, and how variable?
Matheus P. Viana, Jianxu Chen, Theo A. Knijnenburg, Ritvik Vasan, Calysta Yan, Joy E. Arakaki, Matte Bailey, Ben Berry, Antoine Borensztejn, Jackson M. Brown, Sara Carlson, Julie A. Cass, Basudev Chaudhuri, Kimberly R. Cordes Metzler, Mackenzie E. Coston, Zach J. Crabtree, Steve Davidson, Colette M. DeLizo, Shailja Dhaka, Stephanie Q. Dinh, Thao P. Do, Justin Domingus, Rory M. Donovan-Maiye, Tyler J. Foster, Christopher L. Frick, Griffin Fujioka, Margaret A. Fuqua, Jamie L. Gehring, Kaytlyn A. Gerbin, Tanya Grancharova, Benjamin W. Gregor, Lisa Harrylock, Amanda Haupt, Melissa C. Hendershott, Caroline Hookway, Alan R. Horwitz, Chris Hughes, Eric J. Isaac, Gregory R. Johnson, Brian Kim, Andrew N. Leonard, Winnie Leung, Jordan J. Lucas, Susan A. Ludmann, Blair M. Lyons, Haseeb Malik, Ryan McGregor, Gabe E. Medrash, Sean L. Meharry, Kevin Mitcham, Irina A. Mueller, Timothy L. Murphy-Stevens, Aditya Nath, Angelique M. Nelson, Luana Paleologu, T. Alexander Popiel, Megan M. Riel-Mehan, Brock Roberts, Lisa M. Schaefbauer, Magdalena Schwarzl, Jamie Sherman, Sylvain Slaton, M. Filip Sluzewski, Jacqueline E. Smith, Youngmee Sul, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Daniel T. Toloudis, Andrew P. Tucker, Veronica Valencia, Winfried Wiegraebe, Thushara Wijeratna, Ruian Yang, Rebecca J. Zaunbrecher, Allen Institute for Cell Science, Graham T. Johnson, Ruwanthi N. Gunawardane, Nathalie Gaudreault, Julie A. Theriot, Susanne M. Rafelski
Dog color patterns explained by modular promoters of ancient canid origin
Danika L. Bannasch, Christopher B. Kaelin, Anna Letko, Robert Loechel, Petra Hug, Vidhya Jagannathan, Jan Henkel, Petra Roosje, Marjo K. Hytönen, Hannes Lohi, Meharji Arumilli, DoGA consortium, Katie M. Minor, James R. Mickelson, Cord Drögemüller, Gregory S. Barsh, Tosso Leeb
Molecular topography of an entire nervous system
Seth R Taylor, Gabriel Santpere, Alexis Weinreb, Alec Barrett, Molly B. Reilly, Chuan Xu, Erdem Varol, Panos Oikonomou, Lori Glenwinkel, Rebecca McWhirter, Abigail Poff, Manasa Basavaraju, Ibnul Rafi, Eviatar Yemini, Steven J Cook, Alexander Abrams, Berta Vidal, Cyril Cros, Saeed Tavazoie, Nenad Sestan, Marc Hammarlund, Oliver Hobert, David M. Miller III
Automated hiPSC culture and sample preparation for 3D live cell microscopy
Mackenzie E. Coston, Benjamin W. Gregor, Joy Arakaki, Antoine Borensztejn, Thao P. Do, Margaret A. Fuqua, Amanda Haupt, Melissa C. Hendershott, Winnie Leung, Irina A. Mueller, Angelique M. Nelson, Susanne M. Rafelski, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Winfried Wiegraebe, Calysta Yan, Ruwanthi N Gunawardane, Nathalie Gaudreault
Scalable production of tissue-like vascularised liver organoids from human PSCs
Sean P Harrison, Richard Siller, Yoshiaki Tanaka, Yangfei Xiang, Benjamin Patterson, Henning Kempf, Espen Melum, Kathrine S Åsrud, Maria E Chollet, Elisabeth Andersen, Per Morten Sandset, Saphira Baumgarten, Flavio Bonanini, Dorota Kurek, Santosh Mathapati, Runar Almaas, Kulbhushan Sharma, Steven R Wilson, Frøydis S Skottvoll, Ida C Boger, Inger L Bogen, Tuula A Nyman, Jun J Wu, Ales Bezrouk, Dana Cizkova, Jaroslav Mokry, Robert Zweigerdt, In-Hyun Park, Gareth J Sullivan
We live in an ageing society with high incidence of cognitive, sensory and motor decline, as well as neurodegenerative diseases such as Alzheimer’s disease (AD) or Fronto-temporal Dementia (FTD). Although decay of synaptic functions is a clear hallmark of the aforementioned conditions, we know too little about the underlying causes. The overarching aim of this project is to study roles of the ageing- and neurodegenerative disease-related factor Tau during the regulation of synapses in health, ageing and disease.
Synapses are specialised neuronal cell junctions which contain complex machinery for rapid transmission of signals to partner cells. This machinery is frequently disrupted during ageing and in neurodegeneration and the resulting synaptic malfunction is an important cause for cognitive, sensory and motor decline. The underpinning mechanisms are poorly understood. To bridge this knowledge gap, we focus on Tau. Tau plays a vital role in the pathogenesis of neurodegenerative disorders and is also linked to physiological ageing. Accordingly, Tau is an important therapeutic target for the development of treatments of AD and FTD.
Considering Tau’s crucial roles during pathogenesis and treatment of dementia, it is vital to understand its physiological function. Tau loss is known to lead to age-related synaptic deficits both in mice and the fruit fly Drosophila, and our work has started to deliver first explanations. Thus, we have shown that Tau loss triggers aberrations of microtubule networks and axonal transport deficits affecting synapse formation and maintenance (Voelzmann et al., 2016, eLife 5, e14694ff.; Hahn et al., 2020, bioRxiv 2020.08.19.257808ff.). We now find from our proteomic and preliminary functional studies that Tau plays even more direct roles by binding to factors that are important for synaptic function. The aim of this project is to understand these synaptic mechanisms of Tau. For this, we will use the model organism Drosophila which harbours Tau and synaptic machinery that is well conserved with humans, but can be studied far more effectively than in other model organisms. Using Drosophila‘s efficient genetics, powerful experimental strategies and simple robust behavioural assays, you will study the functional links between Tau and its synaptic binding partners. This will involve inter-disciplinary approaches using genetics, molecular biology, biochemistry, cell biology, cell culture and in vivo studies, cutting-edge bioimaging of synaptic activity and behavioural studies. You will unravel mechanisms of Tau at synapses as a means to understand neuronal decay during ageing and in neurodegeneration.
Applications from candidates, ideally with some background in cell biology, genetics, neuroscience and/or biomedical sciences are encouraged to apply. The successful applicant will be based in the Institute of Systems, Molecular & Integrative Biology, University of Liverpool, supervised by Dr Sánchez-Soriano (https://sanchezlab.wordpress.com/research/), whilst working closely with Dr Olena Riabinina (http://insectneurolab.com/) at the Department of Biosciences, Durham University. Interested applicants should contact Dr Sanchez-Soriano to discuss the project: n.sanchez-soriano@liverpool.ac.uk.
A postdoctoral research associate position is available immediately to investigate the role of reactive oxygen species (ROS) as physiological signaling molecules in axonal growth and guidance in the lab of Dr. Daniel M. Suter at Purdue University, West Lafayette, IN https://suterlab.bio.purdue.edu/. Specifically, the work will investigate the molecular and cellular mechanisms of how ROS regulate axonal growth and guidance in vitro and in vivo. The successful applicant has experience in cell biology and molecular biology. Additional expertise in developmental neuroscience, microscopy, and working with zebrafish is desirable but not required. This position is supported through funding from the NIH.
Highly motivated candidates with a PhD in cell biology, molecular biology, or neuroscience who are interested to participate in this exciting project at the interface of cellular and developmental neurobiology are invited to send their CV, a brief statement of research interests and contact information of three referees to Dr. Suter.
Dr. Daniel M. Suter
Department of Biological Sciences
Purdue University
915 West State Street
West Lafayette, IN 47907
USA dsuter@purdue.edu
765-496-1562
The BiOf lab http://biof-lab.org/ has developed a microfluidic technique, the Cellular Capsules Technology, that allows them to produce multicellular spheroids and organoids in a high throughput and controlled format. The applications in tissue engineering, oncology and regenerative medicine and toxicology testing are numerous. The recruited postdocs will be involved in the interdisciplinary projects pursued by the team in engineering multiscale vascularized tissues, reconstructing a functional liver lobule, deciphering the impact of mechanical cues on hepatocarcinoma and glioblastoma progression in vitro models, developing innovative microscopy techniques for thick tissue imaging.
We seek for talented scientists with exceptional motivation and outstanding expertise (i.e. PhD) in biophotonics /image analysis, cancer or stem cell biology, microfluidics, biophysics or tissue engineering.
The proposed funding is for 12 months and can be renewed up to 36 months. The salary will be adapted to the experience of the applicant. Starting date will be between March and June 2021.
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