Today let’s delve into a curious case involving induced pluripotent stem cells (iPSCs) and leukemic stem cells (LSCs). Most blood cells derived from iPSCs are unable to engraft in immunodeficient mice. However, Wesely and colleagues observed an exceptionally high engraftment efficiency of cell lines derived from an individual affected by acute myeloid leukemia (AML). In particular, in vitro they observed typical round cells together with cobblestone-like, firmly adherent cells. The latter displayed markers of immaturity, a more quiescent cell cycle, but foremost they were responsible for the successful engraftment in immunodeficient mice. Collectively, these properties prompted the authors to identify the cells as induced Leukemic Stem Cells (iLSCs). They proved that the iLSCs can become the round cells, but not vice versa, suggesting a stem cell nature. In addition, the adherent phenotype allowed the easy separation of the two populations. In-depth transcriptomic analysis, both at single-cell and population level, was coupled with the study of chromatin accessibility. The iLSCs displayed a molecular resemblance to the leukemic stem cells isolated from AML patients, based on a 42-genes signature. Finally, the authors identified the transcription factor RUNX1 as critical for iLSC phenotype maintenance, as it is involved in the expression of 16 of the 42 genes in the LSC signature. In conclusion, this work describes for the first time the derivation of LSCs from iPSCs. This could be a fantastic tool for the study of the cancer stem cell theory, as those rare cells are not prospectively isolated, but only studied after transplantation. Since these iLSCs were derived from a single patient, it will be interesting to isolate them from other AML-iPSCs lines.

 

Wesely et al. “Acute Myeloid Leukemia iPSCs Reveal a Role for RUNX1 in the Maintenance of Human Leukemia Stem Cells”

Doi: https://doi.org/10.1016/j.celrep.2020.107688

(1 votes)
Loading...

The Korzelius lab has a funded position for a 3-year PhD at the University of Kent in Canterbury, U.K. We use the Drosophila intestine as a model system for age-related decline of organ function. Similar to the mammalian small intestine and colon, the fly intestine is maintained by a population of adult Intestinal Stem Cells (ISCs). Our previous work has identified key transcriptional regulators that govern ISC maintenance and differentiation (Korzelius et al., 2014 EMBO Journal, Korzelius et al., 2019 Nature Communications). Please visit our website for more information: https://www.kent.ac.uk/biosciences/people/3191/www.kent.ac.uk/biosciences/people/3191/korzelius-jerome

This PhD project will investigate how ISC maintenance and differentiation change during aging, focusing on some of the key transcription factors important for ISC maintenance and differentiation. This project will allow you to build skills working with different types of whole genome datasets (RNA-Seq, DamID) as well as building skills in genetics, molecular biology and image analysis. We will e.g. perform crosses with inducible expression of RNAi’s, lifespan assays, microdissection and staining of gut tissue and FACS-isolation of midgut cell populations for whole genome RNA-sequencing.

You will work in the dynamic environment of the School of Biosciences at the University of Kent. The School of Biosciences has a rich expertise on Cancer and Ageing research, and we will have close connections to the ageing-related research in the labs of Dr. Jennifer Tullet and Dr. Marina Ezcurra. You will also be able to work with our collaborators in Germany at renowned institutes such as the Max Planck Institute for Biology of Ageing in Cologne and the FLI-Leibniz Institute on Aging in Jena as well as Genentech Inc. in San Francisco, U.S.A.

We are looking for a curiosity-driving student that works both independently and as part of a team and is interested in a multi-disciplinary research project. The ideal candidate should have knowledge of molecular biology techniques such as DNA/RNA-isolation, PCR and cloning as well as genetics. Additional experience in either Drosophila husbandry and genetics, bio-informatics or immuno-histochemistry and confocal microscopy would be an advantage. Excellent writing and communication skills in English are necessary.

Please send a cover letter and CV and contact details for 2 references. Further details about the application process and the position can be found at https://www.kent.ac.uk/scholarships/search/FN25TRISCA02

(No Ratings Yet)
Loading...

In the latest episode of Genetics Unzipped we’re off on our virtual travels, finding out about the highs and lows of fieldwork. From chasing butterflies up mountains to artificially inseminating kakapos with the help of drones and putting angry birds in paper bags until they poo, we talk to the researchers studying genetics and evolution in action. 

Every year The Genetics Society runs the Heredity Fieldwork Grant scheme, awarding up to £1,500 to cover the travel and accommodation costs for researchers wanting to carry out a fieldwork project in genetics. Our stay-at-home roving reporter Georgia Mills caught up with four intrepid explorers who’ve been off on their travels in locations as exotic as New Zealand, Lanzarote and the Lake District to hear more about their research and what they learned out in the field.

Genetics Unzipped is the podcast from The Genetics Society. Full transcript, links and references available online at GeneticsUnzipped.com

Subscribe from Apple podcasts/iTunes, Spotify and all good podcast apps to make sure you get the latest episodes and catch up on our back catalogue.

If you enjoy the show, please do rate and review on Apple podcasts and help to spread the word on social media. And you can always send feedback and suggestions for future episodes and guests to podcast@geneticsunzipped.com Follow us on Twitter – @geneticsunzip

(No Ratings Yet)
Loading...

This post highlights the approach and finding of a new research article published by Disease Models and Mechanisms (DMM). This feature is written by Olivia Howell as apart of a seminar at The University of Alabama (taught by DMM Editorial Board member, Prof. Guy Caldwell) on current topics related to use of animal and cellular model systems in studies of human disease.

---

 

Within the brain, anomalies in neuronal migration can precipitate aberrant phenotypes such as epilepsy, a disorder in which atypical neuronal circuitry induces recurrent seizures alongside additional neurological abnormalities^2,3. X-linked infantile spasms syndrome (ISSX) is one such debilitating epileptic subtype hallmarked by intellectual disability and intractable seizures that first present in infancy⁴. Previous work has established a causative link between ISSX and mutations in the Aristaless-related homeobox (ARX) – a gene that influences tangential and radial migration of some GABAergic interneurons vital for repressing excitatory neuronal signaling^5,6,7,8. Despite recent progress, the precise pathogenesis of ISSX as well as safe, specific and effective treatments remain elusive. Consequently, characterization of early progenitor interneurons is crucial to understanding and managing this disorder.

In this report, Siehr et al. hypothesized that recapitulating pancreatic ARX functionality within a developing neuronal framework would elucidate the role of this gene in ISSX and the means by which E2 and ACTH mediate their anti-epileptic effects⁷. They therefore utilized an Arx^(GCG)10+7 mouse model that recapitulates the ISSX phenotype to uncover temporally increased levels of apoptosis within the neocortex of Arx-mutant mice. Because this abnormal pattern of apoptosis could not be ascribed to ARX cell death, Siehr et al. deemed it non-cell autonomous in nature. While the affected cell population remains unascertained, Siehr and colleagues have definitively eliminated cortical non-ARX expressing interneurons and inflammatory processes from consideration by examining postnatal neuronal survival and neuroinflammation.

In regard to therapeutics, E2 was found to mitigate ARX⁺ cell density and ISSX seizure phenotype but proved unable to rescue increased apoptosis – rendering the utility of this drug unresolved. Moreover, the unanticipated failure of ACTH to rescue ARX ^(GCG)10+7 mutants from seizure phenotype may ultimately lay the groundwork to model intractable ACTH-resistant ISSX cases and thereby explore alternative ISSX treatments.

Notably, the authors herein report the first known observation of ARX-associated apoptosis in an ARX ^(GCG)10+7 rodent model for ISSX – a corroboration of findings in pancreatic tissue expressing aberrant ARX that highlights the relevance of cross-organ systems research.  While too soon to conclude that apoptosis contributes to ISSX pathogenesis, these results underscore the broad, varied and lingering effects of ARX upon neuronal structure and development. Accordingly, one can expect that subsequent pharmacodynamic studies of E2 and ACTH may ascertain their therapeutic relevance to ISSX while also elucidating the relationship between ARX-mediated apoptosis and subclinical molecular features of ISSX pathology.

 

 

References

1. Siehr, M., Massey, C. and Noebels, J. L. (2020). Arx expansion mutation perturbs cortical development by augmenting apoptosis without activating innate immunity in a mouse model of X-​Linked Infantile Spasms Syndrome. Dis Model Mech 13, 1-10.

2. Hwang, H. M., Ku, R. Y. and Hashimoto-Toril, K. (2019) Prenatal environment that affects neuronal migration. Cell Dev. Biol. 7, 138. 

3. Jackson, M. R., Lee, K., Mattiske, T., Jaehne, E. J., Ozturk, E., Baune, B. T., O’Brien, T. J., Jones, N. and Shoubridge, C. (2017). Extensive phenotyping of two ARX polyalanine expansion mutation mouse models that span clinic al spectrum of intellectual disability and epilepsy. Dis. 105, 245-256. 

4. Olivetti, P. R. and Noebels, J. L. (2012). Interneuron, interrupted: molecular pathogenesis of ARX mutation and X-linked infantile spasms. Opin. Neurobiol. 22, 859-865. 

5. Olivetti, P. R., Maheshwari, A. and Noebels, J. L. (2014). Neonatal estradiol stimulation prevents epilepsy in Arx model of X-linked infantile spasms syndrome. Transl. Med. 6, 1-10. 

6. Poirier, K., Van Esch, H., Friocourt, G., Saillour, Y., Bahi, N., Backer, S., Souil, E., Castelnau-Ptakhine, L., Beldjord, C., Francis, F. et al. (2003) Neuroanatomical distribution of ARX in brain and its localisation in GABAergic neurons. Brain Res. Mol. Brain Res. 122, 35-46. 

8. Wilcox, C. L., Terry, N. A. and May, C. L. (2013). Arx polyalanine expansion in mice leads to reduced pancreatic a -Cell specification and increased a-Cell death. PLoS ONE 8, e78741. 
9. Mattiske, T., Lee, K., Gecz, J., Friocourt, G. and Shoubridge, C. (2016). Embryonic forebrain transcriptome of mice with polyalanine expansion mutations in the ARX homeobox gene. Mol. Genet. 25, 5433-5443.

 

 

 

(No Ratings Yet)
Loading...

This post highlights the approach and finding of a new research article published by Disease Models and Mechanisms (DMM). This feature is written by Joseph I. Kaluzny as apart of a seminar at The University of Alabama (taught by DMM Editorial Board member, Prof. Guy Caldwell) on current topics related to use of animal and cellular model systems in studies of human disease.

---

 

Fibrolamellar Carcinoma (FLC) is a hepatocellular carcinoma that disproportionately affects young patients and is characterized by a fusion transcript, DNAJB1-PRKACA, which acts as a unique molecular driver and is sufficient for diagnosis (Graham et al., 2015). While liver resection and transplantation remain common management approaches (Kassahun, 2016), the lack of available therapy has motivated molecular mechanistic studies of the fusion.

Previous work has shown that the fusion is sufficient to drive FLC tumorigenesis in murine models (Engelholm et al., 2017). In a recent Disease Models & Mechanisms article, de Oliveira and colleagues chose to study the fusion in zebrafish due to their transparent larvae that provide non-invasive live imaging of liver morphology and inflammatory responses (de Oliveira et al., 2020). The researchers used a hepatocyte-specific promoter to overexpress the fusion and establish an FLC zebrafish line. Liver visualization in adults was achieved via outcrossing with a transgenic line expressing agfp-I10a (Fig. 2A in de Oliveira et al., 2020). The livers of 8- and 12-month-old FLC and control fish were resected for standard histopathological evaluation, which confirmed liver enlargement and abnormal hepatocellular architecture in FLC livers (Fig. 2B-C in de Oliveira et al., 2020).

The investigators then sought to determine if overexpression of the fusion caused alterations indicative of malignancy in larval zebrafish. The researchers confirmed hepatomegaly 7 days post-fertilization, suggesting the potential for zebrafish larvae to be used as a model to study the progression of early FLC, an area of interest for a progressive condition that primarily affects young patients (Fig. 3 in de Oliveira et al., 2020). Overexpression of the fusion increased neutrophil and macrophage infiltration into the liver, TNFα-positive macrophages, and caspase-a activity, confirming an inflammatory response in FLC larvae (de Oliveira et al., 2020). Targeting this inflammation with TNFα and caspase-a inhibitors limited FLC progression.

Despite this potential for therapy, there are many outstanding issues with the zebrafish FLC model, such as the presence of two fusion forms due to the genome duplication in zebrafish, the lack of fibrosis markers characteristic of human FLC progression (Kastenhuber et al., 2017), and alternate pro-inflammatory pathways that are unexplored or understudied in the field (Rigutto et al., 2009), which warrant further study.

 

References

de Oliveira, S., Houseright, R. A., Korte, B. G. and Huttenlocher A. (2020). DnaJ-PKAc Fusion Induces Liver Inflammation in a Zebrafish Model of Fibrolamellar Carcinoma. Disease Models & Mechanisms, 26 Feb. 2020, doi:10.1242/dmm.042564.

Engelholm, L. H., Riaz, A., Serra, D., Dagnaes-Hansen, F., Johansen, J. V., Santoni-Rugiu, E., Hansen, S. H., Niola, F. and Frodin, M. (2017). CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1-Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma. Gastroenterology 153, 1662-1673 e10.

Graham, R. P., Jin, L., Knutson, D. L., Kloft-Nelson, S. M., Greipp, P. T., Waldburger, N., Roessler, S., Longerich, T., Roberts, L. R., Oliveira, A. M. et al. (2015). DNAJB1-PRKACA is specific for fibrolamellar carcinoma. Mod Pathol 28, 822-9.

Kassahun, W. T. (2016). Contemporary management of fibrolamellar hepatocellular carcinoma: diagnosis, treatment, outcome, prognostic factors, and recent developments. World J Surg Oncol 14, 151.

Kastenhuber, E. R., Lalazar, G., Houlihan, S. L., Tschaharganeh, D. F., Baslan, T., Chen, C. C., Requena, D., Tian, S., Bosbach, B., Wilkinson, J. E. et al. (2017). DNAJB1-PRKACA fusion kinase interacts with beta-catenin and the liver regenerative response to drive fibrolamellar hepatocellular carcinoma. Proc Natl Acad Sci U S A 114, 13076-13084.

Rigutto, S., Hoste, C., Grasberger, H., Milenkovic, M., Communi, D., Dumont, J. E., Corvilain, B., Miot, F. and De Deken, X. (2009). Activation of dual oxidases Duox1 and Duox2: differential regulation mediated by camp-dependent protein kinase and protein kinase C-dependent phosphorylation. J Biol Chem 284, 6725-34.

 

 

 

 

(No Ratings Yet)
Loading...

EWM2020 meeting is going online June 22nd-23rd.

follow by a Poster Session June 29-30th with new abstract submission

Details  at shorturl.at/mvFY7 & Twitter Ewbank Pujol lab  #EWM2020;  Wormbase

 

 

(1 votes)
Loading...

Author:

Suvimal Kumar Sindhu, Graduate Student @ Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh, 208016, India Email: suvimal.sindhu@gmail.com

 

Having joined my Ph.D. programme at India Institute of Technology Kanpur in India, I always witnessed my senior colleagues defending their Ph.D. thesis with panache and grace; for juniors like me such visuals were highly captivating and motivating. During my Ph.D. I trained to manipulate gene expression and analyze its effect on the developing avian brain. Using these skill-set I created additional hippocampus-like regions (a center of learning and memory) in the chick brain, to understand its development in the brain (Sindhu et al., 2019). I proposed a mechanism for correct positioning of hippocampus in the avian brain. After years of arduous and passionate research work, my defense day was scheduled in the evening of April 17^th and I was thrilled, for I was ready to experience the joy and accomplishment, a vicarious pleasure experienced through my senior colleagues, and sincerely hoping to match the high standards set by them. 

While I was getting prepared for my D-day, the COVID-19 pandemic started to spread in India. Staying in an autonomous and highly secure residential campus, everybody — I know — thought that our work won’t be affected. However, things escalated quickly and in an unprecedented manner, merely two weeks prior to my defense most of the organizations, including my institute, got shut down. COVID-19 took away everybody “reasons for struggle” in life. Now the only possible thing was to “stay at home” and “work from home”. With a broken heart, I came back to my home located in a remote village in the Indian state of Bihar. Amidst all this, I was becoming anxious and restless, my dream to become a Doctor of Philosophy getting delayed till uncertainty. After more than one month of nationwide lockdown, institutions started to conduct virtual academic seminars and meetings. And I got the opportunity to defend my thesis on the last day of the same month – April. I was disheartened as it would not be the same as a traditional thesis defense, which I had always dreamt of, but this was the only option available under these situations.

 

[Image on request designed by www.hoodnscience.com]

Suddenly I realized that there would be logistical hurdles, and preparation in the absence of supervisor and lab-mates would be challenging. But, I was determined to do this and decided to troubleshoot every aspect of it. Considering the location of my home two major hurdles were there -1) frequent power failure of home supply and 2) unstable cellular-network. Arranging an alternative power source and a Wi-Fi device was not a problem; I was not sure whether the network – which is mostly congested under current situation – would stably transmit data needed to sustain a group video conference with around 50 participants.  More importantly, how much data will it consume? – 2GB, 20GB or, 200GB, any number was just a blind guess. To test these conditions a priori, arranging such huge participants that too with a free version of any video conferencing app was just not feasible. I thought of doing a couple of trial presentations to check if my slides are in order and the quality of audio and video are fine. My first mock presentation with my supervisor was quite upsetting; the connection lasted only for 10 minutes as the network was highly unstable. In the second trial, I changed my location within the limited possibilities and that improved the data speed to ~65MBPS. This time my mock presentation lasted for 50 minutes and it consumed ~270MB of data. This was my first experience to deliver a formal presentation online, and hence sometimes I became oblivious of my virtual audience. I had minimized the video thumbnail of participants to avoid cluttering my screen, which was shared with others for the slide show. However, as a trade-off, I lost touch with my audience, and at times felt speaking to myself. This was also due to the fact that to see the entire shared screen running my slide show.

Finally, the D-day arrived, 10 minutes prior to the scheduled time, I signed-in to the Zoom platform and shared my screen; there were ~10 people waiting for my presentation, which eventually rose to 45 people. The connection became unstable; with stuck video and breaking voice, it was a total chaos. I thought my device would not be able to handle the load, and my presentation will have to be postponed. Meanwhile, it was suggested to temporarily turn off only video transmission for the audience except for the oral board members. This idea was quite helpful and I was able to go through my defense presentation as well as discussion, which took around 90 minutes of time. At the end of the session around 1.8GB of data was consumed. Without the slide transition device and laser pointer, it was inconvenient initially, but I adapted to the keyboard and mouse pointer for managing the presentation. To remain in touch with my audience, this time I did not minimize the video of my participants; rather, I kept a few video thumbnails at the corner of my screen. This helped me in remaining aware of their presence and generated a sense of their physical presence.

I had never thought I would defend my thesis this way, but when the whole world is learning a new way to live, I learned a new way to defend and become a doctor under lock down.

 

(Edited by Sahil Batra, Graduate Student @ Indian Institute of Technology Kanpur, India)

 

Sindhu S.K., Udaykumar N., Zaidi M.A.A., Soni A., Sen J. MicroRNA-19b restricts Wnt7b to the hem, which regulates aspects of hippocampus development in the avian forebrain. doi:10.1242/dev.175729, Development, 146, (20):1-7

(23 votes)
Loading...

Welcome to our monthly trawl for developmental biology (and related) preprints. 

---

 

A real monster of a month, May, with masses of preprints uploaded by scientists in various stages of lockdown. Let us know if we missed anything and use these links to get to the section you want:

 

Developmental biology

| Patterning & signalling

| Morphogenesis & mechanics

| Genes & genomes

| Stem cells, regeneration & disease modelling

| Plant development

Evo-devo & evo

Cell biology

Modelling

Tools & resources

Research practice & education

 

 

Developmental biology

| Patterning & signalling

 

Frizzled-dependent Planar Cell Polarity without Wnt Ligands
Joyce J.S. Yu, Aude Maugarny-Calès, Stéphane Pelletier, Cyrille Alexandre, Yohanns Bellaiche, Jean-Paul Vincent, Ian J. McGough

 

 

Hedgehog signalling and lipid metabolism in glia regulate neural stem cell proliferation in Drosophila
Qian Dong, Michael Zavortink, Francesca Froldi, Tammy Lam, Louise Y. Cheng

 

Muscle-derived Myoglianin regulates Drosophila melanogaster imaginal disc growth
Ambuj Upadhyay, Aidan J Peterson, Myung-Jun Kim, Michael B O’Connor

 

Regulation of blood cell transdifferentiation by oxygen sensing neurons in Drosophila
Sean Corcoran, Anjeli Mase, Yousuf Hashmi, Debra Ouyang, Jordan Augsburger, Thea Jacobs, Katelyn Kukar, Katja Brückner

 

Drosophila insulin-like peptide 8 (DILP8) in ovarian follicle cells regulates ovulation and metabolism
Sifang Liao, Dick R. Nässel

 

Rala and the exocyst control Pvr trafficking and signaling to ensure lymph gland homeostasis in Drosophila melanogaster
Helene Knævelsrud, Caroline Baril, Gwenaëlle Gavory, Jorrit M Enserink, Marc Therrien

 

An evolutionarily conserved metallophosphodiesterase is a determinant of lifespan in Drosophila
Kriti Gupta, Vishnu Janardan, Sanghita Banerjee, Sveta Chakrabarti, Swarna Srinivas, Deepthi Mahishi-Vasuki, RAGHU PADINJAT, Sandhya S Visweswariah

 

The oncometabolite L-2-hydroxyglutarate is a common product of Dipteran larval development
Nader H. Mahmoudzadeh, Alexander J. Fitt, Daniel B. Schwab, William E. Martenis, Lauren M. Nease, Charity G. Owings, Garrett J. Brinkley, Hongde Li, Jonathan A. Karty, Sunil Sudarshan, Richard W. Hardy, Armin P. Moczek, Christine J. Picard, Jason M. Tennessen

 

Gap junctions deliver malonyl-CoA from soma to germline to support embryogenesis in Caenorhabditis elegans
Todd A. Starich, David Greenstein

 

 

An extracellular protein regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans
Hui Hong, Huicheng Chen, Yuxia Zhang, Zhimao Wu, Yingying Zhang, Yingyi Zhang, Zeng Hu, Jian Zhang, Kun Lin, Jinghua Hu, Qing Wei

 

Adrenergic activation modulates the signal from the Reissner fiber to cerebrospinal fluid-contacting neurons during development
Yasmine Cantaut Belarif, Margot Penru, Adeline Orts Del’Immagine, Guillaume Pézeron, Claire Wyart, Pierre-Luc Bardet

 

 

Syndecan 2 regulates hematopoietic lineages and infection resolution in zebrafish
Bhawika Sharma Lamichhane, Brent W. Bisgrove, Yi-Chu Su, Bradley L. Demarest, H. Joseph Yost

 

Wnt3 distribution in the zebrafish brain is determined by expression, diffusion and multiple molecular interactions
Sapthaswaran Veerapathiran, Cathleen Teh, Shiwen Zhu, Indira Kartigayen, Vladimir Korzh, Paul T. Matsudaira, Thorsten Wohland

 

 

Proteasome-mediated regulation of Cdhr1a by Siah1 modulates photoreceptor development and survival in zebrafish.
Warlen Piedade, Kayla Titialii-Torres, Ann Morris, Jakub Famulski

 

Wnt16 Elicits a Protective Effect Against Fractures and Supports Bone Repair in Zebrafish
Lucy M. McGowan, Erika Kague, Alistair Vorster, Elis Newham, Stephen Cross, Chrissy L. Hammond

 

 

Light-Sheet Fluorescence Imaging Charts the Gastrula Origin of Vascular Endothelial Cells in Early Zebrafish Embryos
Jing-Wei Xiong, Meijun Pang, Linlu Bai, Weijian Zong, Xu Wang, Ye Bu, Connie Xiong, Jiyuan Zheng, Jieyi Li, Weizheng Gao, Zhiheng Feng, Liangyi Chen, Jue Zhang, Heping Cheng, Xiaojun Zhu

 

Dynamics of primitive streak regression controls the fate of neuro-mesodermal progenitors in the chicken embryo
Charlene Guillot, Arthur Michaut, Brian Rabe, Olivier Pourquié

 

Essential amnion signals for primate primitive streak formation resolved by scRNA map
Ran Yang, Alexander Goedel, Yu Kang, Chenyang Si, Chu Chu, Yi Zheng, Zhenzhen Chen, Peter J. Gruber, Yao Xiao, Chikai Zhou, Chuen-Yan Leung, Yongchang Chen, Jianping Fu, Weizhi Ji, Fredrik Lanner, Yuyu Niu, Kenneth R. Chien

 

 

Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord
Mohammed R Shaker, Ju-Hyun Lee, Kyung Hyun Kim, Veronica Jihyun Kim, Joo Yeon Kim, Ji Yeoun Lee, Woong Sun

 

Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids
Ali Hashmi, Sham Tlili, Pierre Perrin, Alfonso Martinez-Arias, Pierre-François Lenne

 

 

Sonic Hedgehog is not a limb morphogen but acts as a trigger to specify all digits.
Jianjian Zhu, Anna Trofka, Brian D. Harfe, Susan Mackem

 

Regulation of meiotic progression by Sertoli-cell androgen signaling
Hailey Larose, Travis Kent, Qianyi Ma, Adrienne Shami, Jun Li, MaryAnn Handel, Sue Sue Hammoud

 

Developmental Expression of Transforming Growth Factor Induced Protein Promotes NF-Kappa-B Mediated Angiogenesis During Postnatal Lung Development
Min Liu, Cristiana Iosef, Shailaja Rao, Racquel Domingo-Gonzalez, Sha Fu, Paige Snider, Simon J. Conway, Gray S. Umbach, Sarah C. Heilshorn, Ruby E. Dewi, Mar J. Dahl, Donald M. Null, Kurt H. Albertine, Cristina M. Alvira

 

Sox9EGFP defines biliary epithelial heterogeneity downstream of Yap activity
Deepthi Y Tulasi, Diego Martinez Castaneda, Kortney Wager, Karel P Alcedo, Jesse R Raab, Adam D Gracz

 

Striped distribution pattern of Purkinje cells of different birthdates in the mouse cerebellar cortex studied with the Neurog2-CreER transgenic line
Jingyun Zhang, Khoa Tran-Anh, Tatsumi Hirata, Izumi Sugihara

 

Tumor-free elongation of mammalian nephrogenesis by excess fetal GDNF
Hao Li, Jussi Kupari, Yujuan Gui, Edward Siefker, Benson Lu, Kärt Mätlik, Soophie Olfat, Ana R Montaño-Rodríguez, Sung-Ho Huh, Franklin D Costantini, Jaan-Olle Andressoo, Satu Kuure

 

 

FGF signaling regulates development through combinatorial transduction pathways and by modulating cellular adhesion
Ayan Ray, Pierre Mazot, J Richard Brewer, Catarina Catela, Philippe Soriano

 

 

The Notch Ligand Jagged1 is Required for the Formation, Maintenance, and Survival of Hensen Cells in the Mouse Cochlea
Elena Chrysostomou, Luyi Zhou, Yuanzhao L. Darcy, Kaley A. Graves, Angelika Doetzlhofer, Brandon C. Cox

 

MET Receptor Tyrosine Kinase Regulates Vagal Laryngeal Motor Neuron Development and Lifespan Ultrasonic Vocal Communication
Anna K. Kamitakahara, Ramin Ali Marandi Ghoddousi, Alexandra L Lanjewar, Valerie M. Magalong, Hsiao-Huei Wu, Pat Levitt

 

FAM83F regulates canonical Wnt signalling through an interaction with CK1α
Karen Dunbar, Rebecca A Jones, Kevin S Dingwell, Thomas Macartney, James C Smith, Gopal P. Sapkota

 

A gradient of Wnt activity positions the neurosensory domains of the inner ear
Magdalena Żak, Vincent Plagnol, Nicolas Daudet

 

 

Enhanced FGFR3 activity in post-mitotic principal neurons during brain development results in cortical dysplasia and axon miswiring
Jui-Yen Huang, Bruna Baumgarten Krebs, Marisha Lynn Miskus, May Lin Russell, Eamonn Patrick Duffy, Jason Michael Graf, Hui-Chen Lu

 

14-3-3 shuttles Activity-dependent neuroprotective protein to the cytoplasm to promote appropriate neuronal morphogenesis, cortical connectivity and calcium signaling
Sarah A. Bennison, Sara M. Blazejewski, Xiaonan Liu, Kazuhito Toyo-oka

 

Latrophilin-2 repels Teneurin-3+ hippocampal axons during target selection
Daniel T. Pederick, Jan H. Lui, Ellen C. Gingrich, Chuanyun Xu, Yuanyuan Liu, Zhigang He, Stephen R. Quake, Liqun Luo

 

Maturation of complex synaptic connections of layer 5 cortical axons in the posterior thalamic nucleus requires SNAP25
Shuichi Hayashi, Anna Hoerder-Suabedissen, Emi Kiyokage, Catherine Maclachlan, Kazunori Toida, Graham Knott, Zoltán Molnár

 

PEDF-Rpsa-Itga6 signaling regulates cortical neuronal morphogenesis
Sara M. Blazejewski, Sarah A. Bennison, Ngoc Ha, Xiaonan Liu, Trevor H. Smith, Kimberly J. Dougherty, Kazuhito Toyooka

 

A non-linear relation between levels of adult hippocampal neurogenesis and expression of the immature neuron marker doublecortin
Indira Mendez-David, Denis J DAVID, Claudine Delomenie, Martin Beaulieu, Alain M Gardier, Rene Hen

 

Glutathione S-transferase Pi (Gstp) Proteins Regulate Neuritogenesis in the Developing Cerebral Cortex
Xiaonan Liu, Sara M. Blazejewski, Sarah A. Bennison, Kazuhito Toyo-oka

 

Spinal motoneuron firing properties mature from rostral to caudal during post-natal development of the mouse
Calvin Chad Smith, Robert M Brownstone

 

mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex
Madeline G. Andrews, Lakshmi Subramanian, Arnold R. Kriegstein

 

An unconventional cerebrospinal fluid-derived Semaphorin-signalling regulates apical progenitor dynamics in the developing neocortex
Katrin Gerstmann, Karine Kindbeiter, Ludovic Telley, Muriel Bozon, Camille Charoy, Denis Jabaudon, Frédéric Moret, Valerie Castellani

 

Maturation of Purkinje cell firing properties relies on granule cell neurogenesis
Meike E. van der Heijden, Elizabeth P. Lackey, Fatma S. Işleyen, Amanda M. Brown, Ross Perez, Tao Lin, Huda Y. Zoghbi, Roy V. Sillitoe

 

A SMAD1/5-YAP signaling module drives radial glia self-amplification and growth of the developing cerebral cortex
Sonia Najas, Isabel Pijuan, Anna Esteve-Codina, Susana Usieto, Juan D. Martinez, An Zwijsen, Maria L. Arbonés, Elisa Martí, Gwenvael Le Dréau

 

Astrocytic glutamate uptake coordinates experience-dependent, eye-specific refinement in developing visual cortex
Grayson Sipe, Jeremy Petravicz, Rajeev Rikhye, Rodrigo Garcia, Nikolaos Mellios, Mriganka Sur

 

Experience-dependent inhibitory plasticity is mediated by CCK+ basket cells in the developing dentate gyrus
Ting Feng, Christian Alicea, Vincent Pham, Amanda Kirk, Simon Pieraut

 

 

A developmental analysis of juxtavascular microglia dynamics and interactions with the vasculature
Erica Mondo, Shannon C Becker, Amanda G Kautzman, Martina Schifferer, Christina E Baer, Jiapei Chen, Eric J Huang, Mikael Simons, Dorothy P Schafer

 

The floor-plate of His is a non-neuronal electrical conduction pathway
Kalaimakan Herve Arulkandarajah, Guillaume Osterstock, Agathe Lafont, Herve Le Corronc, Nathalie Escalas, Silvia Corsini, Barbara Le Bras, Juliette Boeri, Antonny Czarnecki, Christine Mouffle, Erika Bullier, Elim Hong, Cathy Soula, Pascal Legendre, Jean-Marie Mangin

 

Environmental Oxygen Regulates Astrocyte Proliferation to Guide Angiogenesis during Retinal Development
Robin M Perelli, Matthew L O’Sullivan, Samantha Zarnick, Jeremy N Kay

 

Pharyngeal pouches provide a niche microenvironment for arch artery progenitor specification
Aihua Mao, Linwei Li, Jie Liu, Zhang mingming, Guozhu Ning, Yu Cao, Qiang Wang

 

Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration
Yu-chi Shen, Hailey Larose, Adrienne Niederriter Shami, Lindsay Moritz, Gabriel L. Manske, Qianyi Ma, Xianing Zheng, Meena Sukhwani, Michael Czerwinski, Caleb Sultan, Jourdan Clements, Haolin Chen, Jason R. Spence, Kyle E. Orwig, Michelle Tallquist, Jun Z. Li, Saher Sue Hammoud

 

MEIS-WNT5A axis regulates development of 4th ventricle choroid plexus
Karol Kaiser, Ahram Jang, Melody P. Lun, Jan Procházka, Ondrej Machon, Michaela Procházková, Benoit Laurent, Daniel Gyllborg, Renée van Amerongen, Petra Kompaníková, Feizhen Wu, Roger A. Barker, Ivana Uramová, Radislav Sedláček, Zbyněk Kozmík, Ernest Arenas, Maria K. Lehtinen, Vítězslav Bryja

 

Requirement for Anti-Apoptotic MCL-1 during Early Erythropoiesis
Meghan E. Turnis, Ewa Kaminska, Kaitlyn H. Smith, Brittany J. Kartchner, Peter Vogel, Jonathan D. Laxton, Richard A. Ashmun, Paul A. Ney, Joseph T. Opferman

 

Functional properties of habenular neurons are determined by developmental stage and sequential neurogenesis
Stephanie Fore, Mehmet Ilyas Cosacak, Carmen Diaz Verdugo, Caghan Kizil, Emre Yaksi

 

Preterm birth impedes structural and functional development of cerebellar Purkinje cells in the developing baboon cerebellum
Tara Barron, Jun Hee Kim

 

 

| Morphogenesis & mechanics

 

Furry is required for cell movements during gastrulation and functionally interacts with NDR1
Ailen S Cervino, Bruno Moretti, Carsten Stuckenholz, Hernan E Grecco, Lance A Davidson, Maria Cecilia Cirio

 

Multicellular rosettes organize neuropil formation
Christopher A. Brittin, Anthony Santella, Kristopher Barnes, Mark W. Moyle, Li Fan, Ryan Christensen, Irina Kolotuev, William A. Mohler, Hari Shroff, Daniel A. Colón-Ramos, Zhirong Bao

 

A hydraulic instability drives the cell death decision in the nematode germline
Nicolas T. Chartier, Arghyadip Mukherjee, Julia Pfanzelter, Sebastian Früthauer, Ben T. Larson, Anatol W. Fritsch, Moritz Kreysing, Frank Jülicher, Stephan W Grill

 

Live Imaging of Intracranial Lymphatics in the Zebrafish
Daniel Castranova, Bakary Samasa, Marin Venero Galanternik, Hyun Min Jung, Van N. Pham, Brant M. Weinstein

 

Cell fate coordinates mechano-osmotic forces in intestinal crypt morphogenesis
Qiutan Yang, Shi-Lei Xue, Chii Jou Chan, Markus Rempfler, Dario Vischi, Francisca Mauer Gutierrez, Takashi Hiiragi, Edouard Hannezo, Prisca Liberali

 

Dermomyotome-derived endothelial cells migrate to the dorsal aorta to support hematopoietic stem cell emergence
David Traver, Pankaj Sahai, Claire Pouget, Ondrej Svoboda

 

RASA1-driven cellular export of collagen IV is required for the development of lymphovenous and venous valves in mice
Di Chen, Xin Geng, Philip E. Lapinski, Michael J. Davis, R. Sathish Srinivasan, Philip D. King

 

SIRT1 activity orchestrates ECM expression during hESC-chondrogenic differentiation through SOX5 and ARID5B
Christopher A Smith, Paul A. Humphreys, Nicola Bates, Mark A Naven, Stuart A Cain, Susan J. Kimber, Mona Dvir Ginzbrg

 

Tuba1a is uniquely important for axon guidance through midline commissural structures
Georgia Buscaglia, Jayne Aiken, Katelyn J. Hoff, Kyle R. Northington, Emily A. Bates

 

The actin-modulating protein Synaptopodin mediates long-term survival of dendritic spines
Kenrick Yap, Alexander Drakew, Dinko Smilovic, Michael Rietsche, Mario Vuksic, Domenico Del Turco, Thomas Deller

 

 

Live imaging of hair bundle polarity acquisition in the mouse utricle demonstrates a critical timeline for transcription factor Emx2
Yosuke Tona, Doris K. Wu

 

Endoglycan plays a role in axon guidance and neuronal migration by negatively regulating cell-cell adhesion
Thomas Baeriswyl, Alexandre Dumoulin, Martina Schaettin, Georgia Tsapara, Vera Niederkofler, Denise Helbling, Evelyn Aviles, Jeannine A. Frei, Nicole H. Wilson, Matthias Gesemann, Esther T Stoeckli

 

Modular and distinct PlexinA4/Farp2/Rac1 signaling controls dendrite morphogenesis
Victor Danelon, Ron Goldner, Edward Martinez, Irena Gokhman, Kimberly Wang, Avraham Yaron, Tracy S. Tran

 

Roles of developmentally regulated KIF2A alternative isoforms in cortical neuron migration and differentiation
Cansu Akkaya, Dila Atak, Altug Kamacioglu, Busra Aytul Akarlar, Gokhan Guner, Efil Bayam, Ali Cihan Taskin, Nurhan Ozlu, Gulayse Ince-Dunn

 

Fbxo45 binds SPRY motifs in the extracellular domain of N-cadherin and regulates neuron migration during brain development
Youn Na, Elisa Calvo-Jiménez, Elif Kon, Hong Cao, Yves Jossin, Jonathan A. Cooper

 

Cadherin-11 is required for neural crest determination and survival
Subrajaa Manohar, Alberto Camacho, Crystal D. Rogers

 

Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT
C. Aban, A. Lombardi, G. Neiman, M.C. Biani, A. La Greca, A. Waisman, L.N. Moro, G. Sevlever, S. Miriuka, C. Luzzani

 

Lmo7a Coordinates Neural Crest Migration and Lineage Specification by Regulating Cell Adhesion Dynamics
David Manuel Tatarakis, Adam Tuttle, Thomas F Schilling

 

Attenuation of the extracellular matrix increases the number of synapses but suppresses synaptic plasticity
Yulia Dembitskaya, Nikolay Gavrilov, Igor Kraev, Maxim Doronin, Olga Tyurikova, Alexey Semyanov

 

 

Cadherin Preserves Cohesion Across Involuting Tissues During C. elegans Neurulation
Kristopher Barnes, Li Fan, Mark W. Moyle, Christopher Brittin, Yichi Xu, Daniel Colón-Ramos, Anthony Santella, Zhirong Bao

 

Opposing effects of an F-box protein and the HSP90 chaperone network on microtubule stability and neurite growth in Caenorhabditis elegans
Chaogu Zheng, Emily Atlas, Ho Ming Terence Lee, Susan Laura Javier Jao, Ken C. Q. Nguyen, David H. Hall, Martin Chalfie

 

Role of Drosophila Rap/Fzr (DCdh1) in Retinal Axon Targeting and its Interactions with Loco and Liprin-alpha
Marta Grońska-Pęski, Tadmiri R. Venkatesh

 

A release-and-capture mechanism generates an essential non-centrosomal microtubule array during tube budding
Ghislain Gillard, Gemma Girdler, Katja Röper

 

Robustness of epithelial sealing is an emerging property of local ERK feedbacks driven by cell elimination
Léo Valon, Anđela Davidović, Florence Levillayer, Mathilde Chouly, Fabiana Cerqueira-Campos, Romain Levayer

 

Short distance non-autonomy and intercellular transfer of chitin synthase in Drosophila.
Paul N Adler

 

 

Apico-basal cell compression regulates Lamin A/C levels in Epithelial tissues
K Venkatesan Iyer, Natalie A. Dye, Suzanne Eaton, Frank Jülicher

 

Microtubule-dependent intracellular trafficking promotes apical constriction during tissue invagination
SeYeon Chung, Thao Phuong Le

 

 

Coordinated crosstalk between microtubules and actin by a spectraplakin regulates lumen formation and branching
Delia Ricolo, Sofia J. Araujo

 

Transneuronal Dpr12/DIP-δ interactions facilitate compartmentalized dopaminergic innervation of Drosophila mushroom body axons
Bavat Bornstein, Idan Alyagor, Victoria Berkun, Hagar Meltzer, Fabienne Reh, Hadas Keren-Shaul, Eyal David, Thomas Riemensperger, Oren Schuldiner

 

The transmembrane proteins M6 and Anakonda cooperate to initiate tricellular junction assembly in epithelia of Drosophila
Anna Wittek, Manuel Hollmann, Raphael Schleutker, Stefan Luschnig

 

DAnkrd49 and Bdbt act via Casein Kinase Iε to regulate planar polarity in Drosophila
Helen Strutt, David Strutt

 

A C. elegans Zona Pellucida domain protein functions via its ZPc domain
Jennifer D. Cohen, Jessica G. Bermudez, Matthew C. Good, Meera V Sundaram

 

Cell-extracellular matrix interactions in the fluidic phase direct the topology and polarity of self-organized epithelial structures
Mingxing Ouyang, Jiun-Yann Yu, Yenyu Chen, Linhong Deng, Chin-Lin Guo

 

Size-dependent patterns of cell proliferation and migration in freely-expanding epithelia
Matthew A Heinrich, Ricard Alert, Julienne LaChance, Tom J. Zajdel, Andrej Kosmrlj, Daniel J. Cohen

 

The role of matrix metalloproteinase-9 (MMP-9) in neurodevelopmental deficits and experience-dependent structural plasticity in Xenopus laevis tadpoles
Sayali Gore, Eric J James, Lin-Chien Huang, Jenn J Park, Andrea Berghella, Hollis T Cline, Carlos D Aizenman

 

 

| Genes & genomes

Establishment and maintenance of motor neuron identity via temporal modularity in terminal selector function
Yinan Li, Anthony Osuma, Edgar Correa, Munachi Okebalama, Pauline Dao, Olivia Gaylord, Jihad Aburas, Priota Islam, André E.X. Brown, Paschalis Kratsios

 

C. elegans establishes germline versus soma by balancing inherited histone methylation
Brandon S. Carpenter, Teresa W. Lee, Caroline F. Plott, Jovan S. Brockett, Dexter A. Myrick, David J. Katz

 

 

Cooperation between Caenorhabditis elegans COMPASS and condensin in germline chromatin organization
Marion Herbette, Valerie Robert, Aymeric Bailly, Loic Gely, Robert Feil, David Llères, Francesca Palladino

 

Multidimensional Chromatin Regulation of Cell Lineage Differentiation in a Metazoan Embryo
Zhiguang Zhao, Rong Fan, Weina Xu, Yangyang Wang, Xuehua Ma, Zhuo Du

 

The RNA Polymerase II core subunit RPB-9 directs transcriptional elongation at piRNA loci in Caenorhabditis elegans
Ahmet C. Berkyurek, Giulia Furlan, Lisa Lampersberger, Toni Beltran, Eva-Maria Weick, Emily Nischwitz, Isabela Cunha Navarro, Fabian Braukmann, Alper Akay, Jonathan Price, Falk Butter, Peter Sarkies, Eric A. Miska

 

The impact of genetic background and gender on the increase in mitotic index in response to mating of Drosophila melanogaster
Manashree Malpe, Cordula Schulz

 

Psi promotes Drosophila wing growth through transcriptional repression of key developmental networks
Olga Zaytseva, Naomi C Mitchell, Caroline Delandre, Zuqin Nie, Maurits Evers, Janis K Werner, John T Lis, Ross D Hannan, David L Levens, Owen J Marshall, Leonie M Quinn

 

The nuclear to cytoplasmic ratio directly regulates zygotic transcription in Drosophila through multiple modalities
Sahla Syed, Henry Wilky, João Raimundo, Bomyi Lim, Amanda A. Amodeo

 

An autoregulatory switch in sex-specific phf7 transcription causes loss of sexual identity and tumors in the Drosophila female germline
Anne Smolko, Laura Shapiro-Kulnane, Helen Salz

 

Analysis of gene network bifurcation during optic cup morphogenesis in zebrafish
Lorena Bono, Silvia Naranjo, Tania Moreno-Marmol, Berta de la Cerda, Rocío Polvillo, Francisco Javier Diaz-Corrales, Ozren Bogdanovic, Paola Bovolenta, Juan-Ramon Martinez-Morales

 

Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
Michael Lattke, Robert Goldstone, Francois Guillemot

 

Gene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons.
Dong Won Kim, Kai Liu, Zoe Qianyi Wang, Yi Stephanie Zhang, Abhijith Bathini, Matthew P. Brown, Sonia Hao Lin, Parris W. Washington, Changyu Sun, Susan Lindtner, Bora Lee, Hong Wang, Tomomi Shimogori, John L.R. Rubenstein, Seth Blackshaw

 

Transcriptional and epigenetic characterization of early striosomes identifies Foxf2 and Olig2 as factors required for development of striatal compartmentation and neuronal phenotypic differentiation
Maria-Daniela Cirnaru, Sicheng Song, Kizito-Tshitoko Tshilenge, Chuhyon Corwin, Justyna Mleczko, Carlos Galicia Aguirre, Houda Benlhabib, Jaroslav Bendl, Jordi Creus Muncunill, Pasha Apontes, John F. Fullard, Peter Carlsson, Panos Roussos, Sean D. Mooney, Lisa M. Ellerby, Michelle E. Ehrlich

 

The transcription factor Pou3f1 provides a new map to the glutamatergic neurons of the cerebellar nuclei
Joshua Po Han Wu, Joanna Yeung, Sih-Rong Wu, Huda Zoghbi, Dan Goldowitz

 

Pluripotency factors select gene expression repertoire at Zygotic Genome Activation
Meijiang Gao, Marina Veil, Marcus Rosenblatt, Anna Gebhard, Helge Hass, Lenka Buryanova, Lev Y. Yampolsky, Björn Grüning, Jens Timmer, Daria Onichtchouk

 

Cytoplasmic pool of spliceosome protein SNRNP70 regulates the axonal transcriptome and development of motor connectivity
Nikolas Nikolaou, Patricia Gordon, Fursham Hamid, Richard Taylor, Eugene Makeyev, Corinne Houart

 

Targeted disruption of Pparγ1 promotes trophoblast endoreplication in the murine placenta
Takanari Nakano, Hidekazu Aochi, Masataka Hirasaki, Yasuhiro Takenaka, Koji Fujita, Hiroaki Soma, Hajime Kamezawa, Takahiro Koizumi, Akihiko Okuda, Takayuki Murakoshi, Akira Shimada, Ikuo Inoue

 

Histone lysine crotonylation regulates cell-fate determination of neural stem/progenitor cells by activating bivalent promoters
Chang-Mei Liu, Shang-Kun Dai, Pei-Pei Liu, Hong-Zhen Du, Xiao Liu, Ya-Jie Xu, Cong Liu, Ying-Ying Wang, Zhao-Qian Teng

 

Defining a critical enhancer near Nanog using chromatin-focused approaches identifies RNA Pol II recruitment as required for expression
Puja Agrawal, Steven Blinka, Kirthi Pulakanti, Michael H Reimer, Sridhar Rao

 

3D chromatin organization changes modulate adipogenesis and osteogenesis
Ruo-Han Hao, Yan Guo, Jing Guo, Yu Rong, Shi Yao, Yi-Xiao Chen, Shan-Shan Dong, Dong-Li Zhu, Hao Chen, Tie-Lin Yang

 

The TFIID subunit Taf4 is required for pancreatic beta cell function and identity
Thomas Kleiber, Guillaume Davidson, Gabrielle Mengus, Igor Martianov, Irwin Davidson

 

TET1 dioxygenase is required for FOXA2-associated chromatin remodeling in pancreatic beta-cell differentiation
Xinwei Wu, Jianfang Li, Minjung Lee, Qingping Lan, Jia Li, Yun Huang, De-Qiang Sun, Ruiyu Xie

 

The H3.3 chaperone Hira complex orchestrates oocyte developmental competence
Rowena Smith, Zongliang Jiang, Andrej Susor, Hao Ming, Janet Tait, Marco Conti, Chih-Jen Lin

 

Transcription Co-Factor LBH Is Necessary for Maintenance of Stereocilia Bundles and Survival of Cochlear Hair Cells
Huizhan Liu, Kimberlee P Giffen, Mhamed Grati, Seth Morrill, Yi Li, Xue Zhong Liu, Karoline J Briegel, David Z.Z. He

 

 

Strain-dependent modifier genes determine survival in Zfp423 mice
Wendy A. Alcaraz, Zheng Liu, Phoebe Valdes, Edward Chen, Alan G. Valdovino Gonzalez, Shelby Wade, Cinny Wong, Eunnie Kim, Hsiang-Hua M. Chen, Dorothy Concepcion, Bruce Alan Hamilton

 

A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus
BE Aronson, L Scourzic, V Shah, E Swanzey, A Kloetgen, A Polyzos, A Sinha, A Azziz, I Caspi, J Li, B Pelham-Webb, H Wichterle, A Tsirigos, M Stadtfeld, E Apostolou

 

Conjugated activation of myocardial-specific transcription of Gja5 by a pair of Nkx2-5-Shox2 co-responsive elements
Tianfang Yang, Zhen Huang, Hua Li, Linyan Wang, YiPing Che

 

Genome-wide Studies Reveal the Essential and Opposite Roles of ARID1A in Controlling Human Cardiogenesis and Neurogenesis from Pluripotent Stem Cells
Juli Liu, Sheng Liu, Hongyu Gao, Lei Han, Xiaona Chu, Yi Sheng, Yue Wang, Weinian Shou, Yunlong Liu, Jun Wan, Lei Yang

 

Integrative Single-cell RNA-Seq and ATAC-Seq Analysis of Human Foetal Liver and Bone Marrow Haematopoiesis
Anna Maria Ranzoni, Andrea Tangherloni, Ivan Berest, Simone Giovanni Riva, Brynelle Myers, Paulina M. Strzelecka, Jiarui Xu, Elisa Panada, Irina Mohorianu, Judith B. Zaugg, Ana Cvejic

 

Translatomic database of cortical astroglia across male and female mouse development reveals two distinct developmental phenotypes
Gareth M. Rurak, Stephanie Simard, François Charih, Amanda Van Geel, John Stead, Barbara Woodside, James R. Green, Gianfilippo Coppola, Natalina Salmaso

 

Single cell RNA sequencing reveals the landscape of early female germ cell development
Zheng-Hui Zhao, Jun-Yu Ma, Tie-Gang Meng, Zhen-Bo Wang, Wei Yue, Qian Zhou, Sen Li, Xie Feng, Yi Hou, Heide Schatten, Xiang-Hong Ou, Qing-Yuan Sun

 

Transcriptional Profiling Reveals the Transcription Factor Networks Regulating the Survival of Striatal Neurons
Lin Yang, Zihao Su, Xiaolei Song, Zhenmeiyu Li, Ziwu Wang, Haotian Chen, Zicong Shang, Heng Du, Yan Wen, Guoping Liu, Dashi Qi, Xiaosu Li, Yan You, Zhejun Xu, Zhuangzhi Zhang

 

Single-cell atlas of human developing and azoospermia patients’ testicles reveals the roadmap and defects in somatic microenvironment
LiangYu Zhao, ChenCheng Yao, XiaoYu Xing, Tao Jing, Peng Li, ZiJue Zhu, Chao Yang, Jing Zhai, RuHui Tian, HuiXing Chen, JiaQiang Luo, NaChuan Liu, ZhiWen Deng, XiaoHan Lin, Na Li, Jing Fang, Jie Sun, ChenChen Wang, Zhi Zhou, Zheng Li

 

Single cell resolution regulatory landscape of the mouse kidney highlights cellular differentiation programs and renal disease targets
Zhen Miao, Michael S Balzer, Ziyuan Ma, Hongbo Liu, Junnan Wu, Rojesh Shrestha, Tamas Aranyi, Amy Kwan, Ayano Kondo, Marco Pontoglio, Junhyong Kim, Mingyao Li, Klaus H Kaestner, Katalin Susztak

 

Single cell RNA sequencing reveals regulation of fetal ovary development in the monkey (Macaca fascicularis)
Zheng-Hui Zhao, Chun-Yang Li, Tie-Gang Meng, Yan Wang, Wen-Bo Liu, Ang Li, Yi-Jun Cai, Yi Hou, Heide Schatten, Zhen-Bo Wang, Quin-Yuang Sun, Qiang Sun

 

Cell states beyond transcriptomics: integrating structural organization and gene expression in hiPSC-derived cardiomyocytes
Kaytlyn A. Gerbin, Tanya Grancharova, Rory Donovan-Maiye, Melissa C. Hendershott, Jackson Brown, Stephanie Q. Dinh, Jamie L. Gehring, Matthew Hirano, Gregory R. Johnson, Aditya Nath, Angelique Nelson, Charles M. Roco, Alexander B. Rosenberg, M. Filip Sluzewski, Matheus P. Viana, Calysta Yan, Rebecca J. Zaunbrecher, Kimberly R. Cordes Metzler, Vilas Menon, Sean P. Palecek, Georg Seelig, Nathalie Gaudreault, Theo Knijnenburg, Susanne M. Rafelski, Julie A. Theriot, Ruwanthi N. Gunawardane

 

 

 

| Stem cells, regeneration & disease modelling

 

 

Planarian stem cells sense the identity of missing tissues to launch targeted regeneration
Tisha E. Bohr, Divya A. Shiroor, Carolyn E. Adler

 

β-catenin perturbations control differentiation programs in mouse embryonic stem cells
Elisa Pedone, Mario Failli, Gennaro Gambardella, Rossella De Cegli, Diego di Bernardo, Lucia Marucci

 

Discrete regulation of β-catenin-mediated transcription governs identity of intestinal epithelial stem cells
Costanza Borrelli, Tomas Valenta, Kristina Handler, Karelia Vélez, Giulia Moro, Atefeh Lafzi, Laura de Vargas Roditi, George Hausmann, Andreas E Moor, Konrad Basler

 

Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
John P Russell, Xinhong Lim, Alice Santambrogio, Val Yianni, Yasmine Kemkem, Bruce Wang, Matthew Fish, Scott Haston, Anaëlle Grabek, Shirleen Hallang, Emily J Lodge, Amanda L. Patist, Andreas Schedl, Patrice Mollard, Roeland Nusse, Cynthia L Andoniadou

 

Dual SMAD inhibition and Wnt inhibition enhances the differentiation of induced pluripotent stem cells into Retinal Ganglion cells (iPSC-RGCs)
Venkata R.M. Chavali, Naqi Haider, Sonika Rathi, Vrathasha Vrathasha, Teja Alapati, Jie He, Kamaljot Gill, Sergei Nikonov, Roman Nikonov, Thu T Duong, Devin S McDougald, Joan M. O’Brien, Jason A Mills

 

Developmental co-emergence of cardiac and gut tissues modeled by human iPSC-derived organoids
A.C. Silva, O.B. Matthys, D.A. Joy, M.A. Kauss, V. Natarajan, M.H. Lai, D. Turaga, M. Alexanian, B.G. Bruneau, T.C. McDevitt

 

Single Cell Transcriptomics-guided Antisense Treatment Improves Endoderm Differentiation of iPSCs
Pooja Hor, Vasu Punj, Zea Borok, Amy Ryan, Justin Ichida

 

Tripartite Inhibition of SRC-WNT-PKC Signalling Consolidates Human Naïve Pluripotency
Jonathan Bayerl, Muneef Ayyash, Tom Shani, Yair Manor, Ohad Gafni, Yael Kalma, Alejandro Aguilera-Castrejon, Mirie Zerbib, Hadar Amir, Daoud Sheban, Shay Geula, Nofar Mor, Leehee Weinberger, Vladislav Krupalnik, Bernardo Oldak, Nir Livnat, Shadi Tarazi, Shadi Tawil, Lior Lasman, Suhair Hanna, Noa Novershtern, Dalit Ben-Yosef, Sergey Viukov, Jacob H. Hanna

 

Transcriptional profiling reveals fundamental differences in iPS-derived progenitors of endothelial cells (PECs) versus adult circulating EPCs
Elmira Jalilian, William Raimes

 

Concurrent stem- and lineage-affiliated chromatin programs precede hematopoietic lineage restriction
Fatemeh Safi, Parashar Dhapola, Sarah Warsi, Eva Erlandsson, Ewa Sitnicka, David Bryder, Charlotta Böiers, Ram Krishna Thakur, Göran Karlsson

 

 

Differential Effects of RASA3 Mutations on Hematopoiesis are Profoundly Influenced by Genetic Background and Molecular Variant
Raymond F. Robledo, Steven L. Ciciotte, Joel H. Graber, Yue Zhao, Amy J. Lambert, Babette Gwynn, Nathaniel J. Maki, Lionel Blanc, Luanne L. Peters

 

Unique roles of ATAC and SAGA KAT2A complexes in normal and malignant hematopoiesis
Liliana Arede, Elena Foerner, Selinde Wind, Rashmi Kulkarni, Ana Filipa Domingues, Svenja Kleinwaechter, Shikha Gupta, Elisabeth Scheer, Laszlo Tora, Cristina Pina

 

Spatial confinement and temporal dynamics of selectin ligands enable stable hematopoietic stem cell rolling
Bader Al Alwan, Karmen AbuZineh, Shuho Nozue, Aigerim Rakhmatulina, Mansour Aldehaiman, Asma S. Al-Amoodi, Maged F. Serag, Fajr A. Aleisa, Jasmeen S. Merzaban, Satoshi Habuchi

 

Spatio-temporal analyses of OCT4 expression and fate transitions in human embryonic stem cells
Sirio Orozco-Fuentes, Laura E. Wadkin, Irina Neganova, Majlinda Lako, Rafael A. Barrio, Andrew W. Baggaley, Anvar Shukurov, Nicholas G. Parker

 

Hair follicle stem cell progeny heal blisters while pausing skin development
Yu Fujimura, Mika Watanabe, Kota Ohno, Yasuaki Kobayashi, Shota Takashima, Hideki Nakamura, Hideyuki Kosumi, Yunan Wang, Yosuke Mai, Andrea Lauria, Valentina Proserpio, Hideyuki Ujiie, Hiroaki Iwata, Wataru Nishie, Masaharu Nagayama, Salvatore Oliviero, Giacomo Donati, Hiroshi Shimizu, Ken Natsuga

 

Loss of clathrin heavy chain enhances actin-dependent stiffness of mouse embryonic stem cells
Ridim D Mote, Jyoti Yadav, Surya Bansi Singh, Mahak Tiwari, Shivprasad Patil Patil, Deepa Subramanyam

 

Mechanochemical control of epidermal stem cell divisions by B-plexins
Chen Jiang, Ahsan Javed, Laura Kaiser, Michele M. Nava, Dandan Zhao, Dominique T. Brandt, Javier Fernández-Baldovinos, Luping Zhou, Carsten Höß, Kovilen Sawmynaden, Arkadiusz Oleksy, David Matthews, Lee S. Weinstein, Hermann-Josef Gröne, Carien M. Niessen, Stefan Offermanns, Sara A. Wickström, Thomas Worzfeld

 

Polyamine-controlled proliferation and protein biosynthesis are independent determinants of hair follicle stem cell fate
Kira Allmeroth, Christine S. Kim, Andrea Annibal, Andromachi Pouikli, Carlos Andrés Chacón-Martínez, Christian Latza, Adam Antebi, Peter Tessarz, Sara A. Wickström, Martin S. Denzel

 

Skeletal muscle-derived Human Mesenchymal Stem Cells: influence of different culture conditions on proliferative and myogenic capabilities
Stefano Testa, Carles Sanchez Riera, Ersilia Fornetti, Federica Riccio, Claudia Fuoco, Sergio Bernardini, Jacopo Baldi, Marco Costantini, Maria Laura Foddai, Stefano Cannata, Cesare Gargioli

 

Modeling cellular crosstalk and organotypic vasculature development with human iPSC-derived endothelial cells and cardiomyocytes
Emmi Helle, Minna Ampuja, Alexandra Dainis, Laura Antola, Elina Temmes, Eero Mervaala, Riikka Kivelä

 

Cadherin-13 is a critical regulator of GABAergic modulation in human stem cell derived neuronal networks
Britt Mossink, Jon-Ruben van Rhijn, Shan Wang, Eline J. H. van Hugte, Katrin Linda, Jitske Bak, Anouk H. A. Verboven, Martijn Selten, Alessia Anania, Sophie Jansen, Jason M. Keller, Teun Klein Gunnewiek, Chantal Schoenmaker, Astrid Oudakker, Monica Frega, Hans van Bokhoven, Dirk Schubert, Nael Nadif Kasri

 

Impact of sustained TGFβ receptor inhibition on chromatin accessibility and gene expression in cultured human endometrial MSC
Raffaella Lucciola, Pavle Vrljicak, Caitlin Filby, Saeedeh Darzi, Shanti Gurung, Joanne Muter, Sascha Ott, Jan J Brosens, Caroline E Gargett

 

Derivation of ringed seal (Phoca hispida) tripotent induced pluripotent stem-like cells
Violetta R. Beklemisheva, Aleksei G. Menzorov

 

Direct reprogramming of adult hepatocytes to generate LGR5+ endodermal progenitor
Diana Chaker, Christophe Desterke, Nicolas Moniaux, Tony Ernault, Noufissa Oudrhiri, Jamila Faivre, Ali Turhan, Annelise Bennaceur, Frank Griscelli

 

Dynamics and variability of neuronal subtype responses during regeneration and growth
Jamie A. Havrilak, Layla Al-Shaer, Noor Baban, Nesli Akinci, Michael J. Layden

 

 

Optical control of ERK and AKT signaling promotes axon regeneration and functional recovery of PNS and CNS in Drosophila
Qin Wang, Huaxun Fan, Feng Li, Savanna S Skeeters, Vishnu V Krishnamurthy, Yuanquan Song, Kai Zhang

 

The Drosophila SWI/SNF chromatin-remodeling complexes BAP and PBAP play separate roles in regulating growth and cell fate during regeneration
Yuan Tian, Rachel K. Smith-Bolton

 

 

Regeneration in sponge Sycon ciliatum mimics postlarval development
Anael Soubigou, Ethan G Ross, Yousef Touhami, Nathan Chrismas, Vengamanaidu Modepalli

 

Regenerative growth is constrained by brain tumor to ensure proper patterning in Drosophila
Syeda Nayab Fatima Abidi, Rachel K. Smith-Bolton

 

Pretreatment of aged mice with retinoic acid restores alveolar regeneration via upregulation of reciprocal PDGFRA signaling
Jason J. Gokey, John Snowball, Jenna Green, Marion Waltamath, Jillian J. Spinney, Katharine E. Black, Lida P. Hariri, Yan Xu, Anne-Karina T. Perl

 

Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
Vikrant Borse, Matthew Barton, Harry Arndt, Tejbeer Kaur, Mark E. Warchol

 

Bioengineered human skeletal muscle with a Pax7+ satellite cell niche capable of functional regeneration
J.W. Fleming, A.J. Capel, R.P. Rimington, P. Wheeler, O.G. Davies, M.P. Lewis

 

Phenotypic screening using synthetic CRISPR gRNAs reveals pro-regenerative genes in spinal cord injury
Marcus Keatinge, Themistoklis M. Tsarouchas, Tahimina Munir, Juan Larraz, Davide Gianni, Hui-Hsin Tsai, Catherina G. Becker, David A. Lyons, Thomas Becker

 

Satellite glial cells promote regenerative growth in sensory neurons
Oshri Avraham, Pan-Yue Deng, Sara Jones, Rejji Kuruvilla, Clay F Semenkovich, Vitaly A Klyachko, Valeria Cavalli

 

Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cord
Lindsay M Milich, James S Choi, Christine B Ryan, Stephanie L Yahn, Pantelis Tsoulfas, Jae Kyu Lee

 

New oligodendrocytes exhibit more abundant and accurate myelin regeneration than those that survive demyelination
Sarah A Neely, Jill M Williamson, Anna Klingseisen, Lida Zoupi, Jason J Early, Anna Williams, David A Lyons

 

Intravital imaging reveals cell cycle-dependent satellite cell migration during muscle regeneration
Yumi Konagaya, Kanako Takakura, Maina Sogabe, Anjali Bisaria, Chad Liu, Tobias Meyer, Atsuko Sehara-Fujisawa, Michiyuki Matsuda, Kenta Terai

 

Proliferation is a requirement for differentiation of oligodendrocyte progenitor cells during CNS remyelination
Sarah Foerster, Björn Neumann, Crystal McClain, Ludovica Di Canio, Civia Z Chen, Daniel S Reich, Benjamin D Simons, Robin JM Franklin

 

Axon-dependent expression of YAP/TAZ mediates Schwann cell remyelination but not proliferation after nerve injury
Matthew Grove, Hyunkyoung Lee, Huaqing Zhao, Young-Jin Son

 

Regenerating motor neurons prime muscle stem cells for myogenesis by enhancing protein synthesis and mitochondrial bioenergetics
Jeongmoon J. Choi, Eun Jung Shin, Woojin M. Han, Shannon E. Anderson, Mahir Mohiuddin, Nan Hee Lee, Thu Tran, Shadi Nakhai, Hyeonsoo Jeong, Anna Shcherbina, Gunjae Jeong, Dong Gun Oh, Laura Weinstock, Sitara B. Sankar, Molly E. Ogle, Lida Katsimpardi, Tata Nageswara Rao, Levi B. Wood, Carlos A. Aguilar, Amy J. Wagers, Young C. Jang

 

Modeling human TBX5 haploinsufficiency predicts regulatory networks for congenital heart disease
Irfan S. Kathiriya, Kavitha S. Rao, Giovanni Iacono, W. Patrick Devine, Andrew P. Blair, Swetansu K. Hota, Michael H. Lai, Bayardo I. Garay, Reuben Thomas, Henry Z. Gong, Lauren K. Wasson, Piyush Goyal, Tatyana Sukonnik, Gunes A. Akgun, Laure D. Bernard, Brynn N. Akerberg, Fei Gu, Kai Li, William T. Pu, Joshua M. Stuart, Christine E. Seidman, J. G. Seidman, Holger Heyn, Benoit G. Bruneau

 

STX18-AS1 is a Long Noncoding RNA predisposing to Atrial Septal Defect via downregulation of NKX2-5 in differentiating cardiomyocytes
Yingjuan Liu, Mun-kit Choy, Sabu Abraham, Gennadiy Tenin, Graeme C. Black, Bernard Keavney

 

 

The Axenfeld-Rieger syndrome gene FOXC1 contributes to left-right patterning
Paul W. Chrystal, Curtis R. French, Francesca Jean, Serhiy Havrylov, Suey van Baarle, Ann-Marie Peturson, Pengfei Xu, J. Gage Crump, David B. Pilgrim, Ordan J. Lehmann, Andrew J. Waskiewicz

 

A ubiquitin-based mechanism for the oligogenic inheritance of heterotaxy and heart defects
Jennifer H Kong, Cullen B Young, Ganesh V Pusapati, Chandni B Patel, Sebastian Ho, Arunkumar Krishnan, Jiuann-Huey Ivy Lin, William Devine, Anne Moreau de Bellaing, Tejas S Athni, L Aravind, Teresa M Gunn, Cecilia W Lo, Rajat Rohatgi

 

Alterations in neuronal physiology, development, and function associated with a common duplication of chromosome 15 involving CHRNA7
Kesavan Meganathan, Ramachandran Prakasam, Dustin Baldridge, Paul Gontarz, Bo Zhang, Fumihiko Urano, Azad Bonni, James E. Huettner, John N. Constantino, Kristen L. Kroll

 

A KCNQ2 variant causing Early Onset Epileptic Encephalopathy increases spontaneous network-driven activity and excitability of pyramidal cells in the layer II/III and V of the motor cortex during a limited period of development
Najoua Biba, Marie Kurz, Laurent Villard, Mathieu Milh, Helene Becq, Laurent Aniksztejn

 

Interpreting the pathogenicity of Joubert Syndrome missense variants in Caenorhabditis elegans
Karen I. Lange, Sofia Tsiropoulou, Katarzyna Kucharska, Oliver E. Blacque

 

A CRISPR/Cas9-Based Approach For Editing Immortalised Human Myoblasts To Model Duchenne Muscular Dystrophy In Vitro
Patricia Soblechero-Martin, Edurne Albiasu-Arteta, Aina Anton-Martinez, Iker Garcia-Jimenez, Gabriela Gonzalez-Iglesias, Irene Larranaga-Aiestaran, Andrea Lopez-Martinez, Javier Poyatos-Garcia, Estibaliz Ruiz-Del-Yerro, Federico Gonzalez, Virginia Arechavala-Gomeza

 

Modelling the developmental spliceosomal craniofacial disorder Burn-McKeown syndrome using induced pluripotent stem cells
Katherine A Wood, Charlie F Rowlands, Huw B Thomas, Steven Woods, Julieta O’Flaherty, Sofia Douzgou, Susan J Kimber, William G Newman, Raymond O’Keefe

 

Cell-type-specific synaptic imbalance and disrupted homeostatic plasticity in cortical circuits of ASD-associated Chd8 haploinsufficient mice
Robert A Ellingford, Emilie Rabeshala de Meritens, Raghav Shaunak, Liam Naybour, M. Albert Basson, Laura C Andreae

 

Neurodevelopmental deficits and cell-type-specific transcriptomic perturbations in a mouse model of HNRNPU haploinsufficiency
Sarah A. Dugger, Ryan S. Dhindsa, Gabriela De Almeida Sampaio, Elizabeth E. Rafikian, Sabrina Petri, Verity A. Letts, Jiaiie Teoh, Junqiang Ye, Sophie Colombo, Mu Yang, Michael J. Boland, Wayne N. Frankel, David B. Goldstein

 

Multiple sclerosis iPSC-derived oligodendroglia conserve their intrinsic properties to functionally interact with axons and glia in vivo
Sabah Mozafari, Laura Starost, Blandine Manot-Saillet, Beatriz Garcia-Diaz, Yu Kang T. Xu, Delphine Roussel, Marion J. F. Levy, Linda Ottoboni, Kee-Pyo Kim, Hans R. Schöler, Timothy E. Kennedy, Jack P. Antel, Gianvito Martino, Maria Cecilia Angulo, Tanja Kuhlmann, Anne Baron-Van Evercooren

 

Antiviral signalling in human IPSC-derived neurons recapitulates neurodevelopmental disorder phenotypes
Katherine Warre Cornish, Leo Perfect, Roland Nagy, Matthew J Reid, Annett Mueller, Amanda Evans, Cédric Ghevaert, Grainne McAlonan, Eva Loth, Declan Murphy, Deepak P Srivastava, Jack Price

 

MRI-guided histology of TDP-43 knock-in mice implicates parvalbumin interneuron loss, impaired neurogenesis and aberrant neurodevelopment in ALS-FTD
Ziqiang Lin, Eugene Kim, Mohi Ahmed, Gang Han, Camilla Simmons, Yushi Redhead, Jack Bartlett, Luis Emiliano Pena Altamira, Isobel Callaghan, Matthew White, Nisha Singh, Stephen Sawiak, Tara Spires-Jones, Anthony C Vernon, Michael Coleman, Jeremy B A Green, Christopher Henstridge, Jeff S Davies, View ORCID ProfileDiana Cash, Jemeen Sreedharan

 

 

| Plant development

 

Shoot and root thermomorphogenesis are linked by a developmental trade-off
Christophe Gaillochet, Yogev Burko, Matthieu Pierre Platre, Ling Zhang, Jan Simura, Vinod Kumar, Karin Ljung, Joanne Chory, Wolfgang Busch

 

Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana
Ivan Kashkan, Mónika Hrtyan, Roberta Filepová, Zuzana Vondráková, Jan Hejátko, Sibu Simon, Debbie Rombaut, Thomas B. Jacobs, Mikko J. Frilander, Jiří Friml, Jan Petrášek, Kamil Růžička

 

 

Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi Network
Yoko Ito, Nicolas Esnay, Matthieu Pierre Platre, Lise C. Noack, Wilhelm Menzel, Stéphane Claverol, Patrick Moreau, Yvon Jaillais, Yohann Boutté

 

Gene Expression Changes Occurring at Bolting Time are Associated with Leaf Senescence in Arabidopsis
Will E Hinckley, Judy A Brusslan

 

 

VST Family Proteins are Regulators of Root System Architecture in Rice and Arabidopsis
Yanlin Shao, Kevin R Lehner, Hongzhu Zhou, Isaiah W Taylor, Chuanzao Mao, Philip N Benfey

 

Nature and effective range of non-cell autonomous activator and inhibitor peptides specifying plant stomatal patterning
Scott Zeng, Emily K. W. Lo, Bryna J. Hazelton, Miguel F. Morales, Keiko U. Torii

 

Brassinosteroids Influence Arabidopsis Hypocotyl Graviresponses Through Changes In Mannans And Cellulose
Marc Somssich, Filip Vandenbussche, Alexander Ivakov, Norma Funke, Colin Ruprecht, Kris Vissenberg, Dominique Van Der Straeten, Staffan Persson, Dmitry Suslov

 

 

AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase Subunit, is critical for pollen tube growth in Arabidopsis
Nick Desnoyer, Greg Howard, Emma Jong, Ravishankar Palanivelu

 

Transcriptome and translatome changes in germinated pollen under heat stress uncover roles of transporter genes involved in pollen tube growth.
Laetitia Poidevin, Javier Forment, Dilek Unal, Alejandro Ferrando

 

Functional Characterization of fer-ts, a ts- FERONIA Mutant Allele That Alters Root Hair Growth
Daewon Kim, Fangwei Gu, Sungjin Park, Jonathon Combs, Alexander Adams, Heather B. Mayes, Erik Nielsen

 

Nighttime gibberellin biosynthesis is influenced by fluctuating environmental conditions and contributes to growth adjustments of Arabidopsis leaves
Putri Prasetyaningrum, Lorenzo Mariotti, Maria Cristina Valeri, Giacomo Novi, Stijn Dhondt, Dirk Inzé, Pierdomenico Perata, Hans van Veen

 

Arabidopsis SMN2/HEN2, Encoding DEAD-box RNA Helicase, Governs Proper Expression of the Resistance Gene SMN1/RPS6 and Is Involved in Dwarf, Autoimmune Phenotypes of mekk1 and mpk4 Mutants
Momoko Takagi, Naoki Iwamoto, Yuta Kubo, Takayuki Morimoto, Hiroki Takagi, Fuminori Takahashi, Takumi Nishiuchi, Keisuke Tanaka, Teruaki Taji, Hironori Kaminaka, Kazuo Shinozaki, Kazuya Akimitsu, Ryohei Terauchi, Ken Shirasu, Kazuya Ichimura

 

Asymmetric Expression of Argonautes in Arabidopsis Reproductive Tissues
PE Jullien, DMV Bonnet, N Pumplin, JA Schroeder, O Voinnet

 

Removal of repressive histone marks creates epigenetic memory of recurring heat in Arabidopsis
Nobutoshi Yamaguchi, Satoshi Matsubara, Kaori Yoshimizu, Motohide Seki, Kouta Hamada, Mari Kamitani, Yuko Kurita, Soichi Inagaki, Takamasa Suzuki, Eng-Seng Gan, Taiko To, Tetsuji Kakutani, Atsushi J Nagano, Akiko Satake, Toshiro Ito

 

iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thaliana.
Mehdi Doumane, Leia Colin, Alexis Lebecq, Aurelie Fangain, Joseph Bareille, Olivier Hamant, Youssef Belkhadir, Yvon Jaillais, Marie-Cecile Caillaud

 

Network analysis prioritizes DEWAX and ICE1 as the candidate genes for two major eQTL hotspots in seed germination
Margi Hartanto, Ronny V. L. Joosen, Basten L. Snoek, Leo A. J. Willems, Mark G. Sterken, Dick de Ridder, Henk W. M. Hilhorst, Wilco Ligterink, Harm Nijveen

 

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress
Nuno M. Gonçalves, Telma Fernandes, Cátia Nunes, Margarida T.G. Rosa, Cleverson C. Matiolli, Mafalda A.A. Rodrigues, Pedro M. Barros, M. Margarida Oliveira, Isabel A. Abreu

 

 

The rice pentatricopeptide repeat protein PPR756 is involved in pollen development by affecting multiple RNA editing in mitochondria
Qiannan Zhang, Yanghong Xu, Jishuai Huang, Kai Zhang, Haijun Xiao, Xiaojian Qin, Linlin Zhu, Yingguo Zhu, Jun Hu

 

Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize
Chong Teng, Han Zhang, Reza Hammond, Kun Huang, Blake Meyers, Virginia Walbot

 

Cis-regulatory elements within TEs can influence expression of nearby maize genes
Jaclyn M Noshay, Alexandre P Marand, Sarah N Anderson, Peng Zhou, Maria Katherine Mejia Guerra, Zefu Lu, Christine O’Connor, Peter A Crisp, Candice N. Hirsch, Robert J Schmitz, Nathan M Springer

 

Ppd-1 Remodels Spike Architecture by Regulating Floral Development in wheat
Yangyang Liu, Lili Zhang, Michael Melzer, Liping shen, Ziying Wang, Zhiwen Sun, Thorsten Schnurbusch, Zifeng Guo

 

Functional analysis of the teosinte branched 1 genes in the tetraploid switchgrass (Panicum virgatum L.) by CRISPR/Cas9-directed mutagenesis
YANG LIU, Weiling Wang, Bing Yang, Christopher Currey, Shui-zhang Fei

 

Organogenesis and Vasculature of Anaxagorea Revealing the Axial Homologs in the Carpel and its Implications for the Origin of Angiosperms
Ya Li, Wei Du, Shuai Wang, Xiao-Fan Wang

 

A heat-shock inducible system for flexible gene expression in cereals
Sophie A Harrington, Anna E. Backhaus, Samantha Fox, Christian Rogers, Philippa Borrill, Cristobal Uauy, Annis Richardson

 

 

 

Evo-devo & evo

An updated staging system for cephalochordate development: one table suits them all
João E Carvalho, François Lahaye, Luok Wen Yong, Jenifer C Croce, Hector Escriva, Jr-Kai Yu, Michael Schubert

 

Brachyury controls Ciona notochord fate as part of a feedforward network and not as a unitary master regulator
Wendy M Reeves, Kotaro Shimai, Konner M Winkley, Michael T Veeman

 

Non-thalamic origin of zebrafish sensory relay nucleus: convergent evolution of visual pathways in amniotes and teleosts
Solal Bloch, Hanako Hagio, Manon Thomas, Aurelie Heuze, Jean-Michel Hermel, Elodie Lasserre, Ingrid Colin, Kimiko Saka, Pierre Affaticati, Arnim Jenett, Koichi Kawakami, Naoyuki Yamamoto, Kei Yamamoto

 

Opsin gene expression in larval and adult deep-sea fishes supports a conserved cone-to-rod pathway in teleost visual development
Nik Lupše, Fabio Cortesi, Marko Freese, Lasse Marohn, Jan-Dag Pohlman, Klaus Wysujack, Reinhold Hanel, Zuzana Musilova

 

Evolutionary Changes in Left-Right Visceral Asymmetry in Astyanax Cavefish
Li Ma, Mandy Ng, Janet Shi, Aniket V. Gore, Daniel Castranova, Brant M. Weinstein, William R. Jeffery

 

 

Evolutionary and homeostatic changes in morphology of visual dendrites of Mauthner cells in Astyanax blind cavefish
Zainab Tanvir, Daihanna Rivera, Kristen Severi, Gal Haspel, Daphne Soares

 

 

How spiders make their eyes: Systemic paralogy and function of retinal determination network homologs in arachnids
Guilherme Gainett, Jesús A. Ballesteros, Charlotte R. Kanzler, Jakob T. Zehms, John M. Zern, Shlomi Aharon, Efrat Gavish-Regev, Prashant P. Sharma

 

Tim29 is required for stem cell activity during regeneration in the flatworm Macrostomum lignano
Stijn Mouton, Kirill Ustyantsev, Frank Beltman, Lisa Glazenburg, Eugene Berezikov

 

Multiple roles for laccase2 in butterfly wing pigmentation, scale development, and cuticle tanning
Ceili L. Peng, Anyi Mazo-Vargas, Benjamin J. Brack, Robert D. Reed

 

 

The gene cortex controls scale colour identity in Heliconius
Luca Livraghi, Joseph J. Hanly, Ling Sheng Loh, Anna Ren, Ian A. Warren, Carolina Concha, Charlotte Wright, Jonah M. Walker, Jessica Foley, Henry Arenas-Castro, Lucas Rene Brenes, Arnaud Martin, W. Owen McMillan, Chris D. Jiggins

 

Sexually dimorphic traits and male-specific differentiation are actively regulated by Doublesex during specific developmental windows in Nasonia vitripennis
Yidong Wang, Anna Rensink, Ute Fricke, Megan C. Riddle, Carol Trent, Louis van de Zande, Eveline C. Verhulst

 

Choanoflagellates and the ancestry of neurosecretory vesicles
Ronja Göhde, Benjamin Naumann, Davis Laundon, Cordelia Imig, Kent McDonald, Benjamin H. Cooper, Frederique Varoqueaux, Dirk Fasshauer, Pawel Burkhardt

 

Short-term heritable variation overwhelms two hundred generations of mutational variance for metabolic traits in Caenorhabditis elegans
Charles F. Baer, Dan Hahn, Lindsay M Johnson, Olivia J Smith

 

Tracing animal genomic evolution with the chromosomal-level assembly of the freshwater sponge Ephydatia muelleri
Nathan James Kenny, Warren R Francis, Ramón E Rivera-Vicéns, Ksenia Juravel, Alex de Mendoza, Cristina Díez-Vives, Ryan Lister, Luis Bezares-Calderon, Lauren Grombacher, Maša Roller, Lael D Barlow, Sara Camilli, Joseph F Ryan, Gert Wörheide, April L Hill, Ana Riesgo, Sally Leys

 

Convergence in sympatry: evolution of blue-banded wing pattern in Morpho butterflies
Violaine LLAURENS, Yann Le Poul, Agathe Puissant, Camille Nous, Patrick Blandin, Vincent Debat

 

Genetic and environmental canalization are not associated among altitudinally varying populations of Drosophila melanogaster
Maria Pesevski, Ian Dworkin

 

Phenotypes to remember: Evolutionary developmental memory capacity and robustness
András Szilágyi, Péter Szabó, Mauro Santos, Eörs Szathmáry

 

 

Cell biology

 

Microtubule re-organization during female meiosis in C. elegans
Ina Lantzsch, Che-Hang Yu, Hossein Yazdhkasti, Norbert Lindow, Erik Szentgyoergyi, Steffen Prohaska, Martin Srayko, Sebastian Fuerthauer, Stefanie Redemann

 

Microtubules originate asymmetrically at the somatic Golgi and are guided via Kinesin2 to maintain polarity within neurons
Amrita Mukherjee, Paul Brooks, Fred Bernard, Antoine Guichet, Paul T. Conduit

 

 

Giantin is required for intracellular N-terminal processing of type I procollagen
Nicola Stevenson, Dylan Bergen, Chrissy L Hammond, David John Stephens

 

Myc-dependent cell competition and proliferative response requires induction of the ribosome biogenesis regulator Peter Pan
Norman Zielke, Anna Vähärautio, Jianping Liu, Jussi Taipale

 

Drosophila phosphatidylinositol-4 kinase fwd promotes mitochondrial fission and can suppress Pink1/parkin phenotypes
Ana Terriente-Felix, Emma L Wilson, Alexander J Whitworth

 

Polarized endosome dynamics engage cytosolic Par-3 and dynein during asymmetric division
Xiang Zhao, Kai Tong, xingye Chen, Bin Yang, Qi Li, Zhipeng Dai, Xiaoyu Shi, Ian Seiple, Bo Huang, Su Guo

 

Hematopoietic progenitors polarize in contact with bone marrow stromal cells by engaging CXCR4 receptors.
Thomas Bessy, Benoit Souquet, Benoit Vianay, Alexandre Schaeffer, Thierry Jaffredo, Jerome Larghero, Laurent Blanchoin, Stephane Brunet, Lionel Faivre, Manuel Thery

 

 

Quantitative live-cell imaging yields novel insight into endogenous WNT/CTNNB1 signaling dynamics
Saskia M.A. de Man, Gooitzen Zwanenburg, Mark A. Hink, Renée van Amerongen

 

EHBP1 and EHD2 regulate Dll4 caveolin-mediated endocytosis during blood vessel development
Amelia M Webb, Caitlin R Francis, Jayson M Webb, Hayle Kincross, Keanna M Lundy, Rachael Judson, Dawn Westhoff, Stryder M Meadows, Erich J Kushner

 

Gene expression and chromatin accessibility during progressive EMT and MET linked to dynamic CTCF engagement
Kelsey S Johnson, Shaimaa Hussein, Shuxuan Song, Priyanka Chakraborty, Mohit Kumar Jolly, Michael Toneff, Yin Lin, Joe Taube

 

Complementary and divergent roles for Ctage5 and Tango1 in zebrafish
Eric M. Clark, Brian A. Link

 

Stable kinetochore–microtubule attachments restrict MTOC position and spindle elongation in acentrosomal oocytes
Aurélien Courtois, Shuhei Yoshida, Tomoya S. Kitajima

 

Phototaxis of the unicellular red alga Cyanidioschyzon merolae is mediated by novel actin-driven tentacles
Sascha Maschmann, Karin Ruban, Johanna Wientapper, Wilhelm J. Walter

 

Arp2/3 complex-mediated actin assembly drives microtubule-independent motility and phagocytosis in the evolutionarily divergent amoeba Naegleria
Katrina B Velle, Lillian K Fritz-Laylin

 

Wnt-inducible Lrp6-APEX2 Interacting Proteins Identify ESCRT Machinery and Trk-Fused Gene as Components of the Wnt Signaling Pathway
Gabriele Colozza, Yasaman Jami-Alahmadi, Alyssa Dsouza, Nydia Tejeda-Muñoz, Lauren V. Albrecht, Eric Sosa, James A. Wohlschlegel, Edward M. De Robertis

 

Myosin 10 and a Cytoneme-Localized Ligand Complex Promote Morphogen Transport
Eric T. Hall, Daniel P. Stewart, Miriam Dillard, Ben Wagner, April Sykes, Jamshid Temirov, Richard E. Cheney, Motomi Mori, Camenzind G. Robinson, Stacey K. Ogden

 

Temporal correlation between oscillating force dipoles drives single cell migration in 3D
A. Godeau, M. Leoni, J. Comelles, H. Delanoe-Ayari, A. Ott, S. Harlepp, P. Sens, D. Riveline

 

Spatial Regulation of MCAK Promotes Cell Polarization and Focal Adhesion Turnover to Drive Robust Cell Migration
Hailing Zong, Mark Hazelbaker, Christina Moe, Stephanie C. Ems-McClung, Ke Hu, Claire E. Walczak

 

Myosin-X is required for integrin activation at filopodia tips
Mitro Miihkinen, Max L.B. Grönloh, Helena Vihinen, Eija Jokitalo, Benjamin Thomas Goult, Johanna Ivaska, Guillaume Jacquemet

 

Cep57 and Cep57L1 cooperatively maintain centriole engagement during interphase to ensure proper centriole duplication cycle
Kei K Ito, Koki Watanabe, Haruki Ishida, Kyohei Matsuhashi, Takumi Chinen, Shoji Hata, Daiju Kitagawa

 

Mitochondria form contact sites with the nucleus to couple pro-survival retrograde response
Radha Desai, Daniel A East, Liana Hardy, James Crosby, Manuel Rigon, Danilo Faccenda, María Soledad Alvarez, Aarti Singh, Marta Mainenti, Laura Kuhlman Hussey, Robert Bentham, Gyorgy Szabadkai, Valentina Zappulli, Gurtej Dhoot, Lisa E Romano, Xia Dong, Isabelle Coppens, Anne Hamacher-Brady, J Paul Chapple, Rosella Abeti, Roland A. Fleck, Gema Vizcay-Barrena, Kenneth Smith, Michelangelo Campanella

 

The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation
Emma Carley, Rachel M. Stewart, Abigail Zieman, Amanda Zubek, Samantha Lin, Valerie Horsley, Megan C. King

 

Novel cytokinetic ring components drive negative feedback in cortical contractility
Kathryn Rehain Bell, Michael E. Werner, Anusha Doshi, Daniel B. Cortes, Adam Sattler, Thanh Vuong-Brender, Michel Labouesse, Amy Shaub Maddox

 

Non-muscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli
Colbie R. Chinowsky, Julia A. Pinette, Leslie M. Meenderink, Matthew J. Tyska

 

Protocadherin 7 localizes to the plasma membrane during mitosis and promotes cytokinesis by a palmitoylation-dependent mechanism
Nazlı Ezgi Özkan-Küçük, Mohammad Haroon Qureshi, Berfu Nur Yiğit, Altuğ Kamacıoğlu, Nima Bavili, Alper Kiraz, Nurhan Özlü

 

 

Modelling

 

Autocrine inhibition of cell motility can drive epithelial branching morphogenesis in absence of growth
Elisabeth G. Rens, Mathé T. Zeegers, Iraes Rabbers, András Szabó, Roeland M.H. Merks

 

Self-organization of cortical areas in the development and evolution of neocortex: a network growth model
Nabil Imam, Barbara Finlay

 

Theory of mechano-chemical patterning and optimal migration in cell monolayers
Daniel Boocock, Naoya Hino, Natalia Ruzickova, Tsuyoshi Hirashima, Edouard Hannezo

 

From heterogenous morphogenetic fields to homogeneous regions as a step towards understanding complex tissue dynamics
Satoshi Yamashita, Boris Guirao, Francois Graner

 

Mechanical cell competition in heterogeneous epithelial tissues
R. J. Murphy, P. R. Buenzli, R. E. Baker, M. J. Simpson

 

A Biophysical Model for Plant Cell Plate Development
Muhammad Zaki Jawaid, Rosalie Sinclair, Daniel Cox, Georgia Drakakaki

 

Coordinating cell polarization and morphogenesis through mechanical feedback
Samhita P. Banavar, Michael Trogdon, Brian Drawert, Tau-Mu Yi, Linda R. Petzold, Otger Campàs

 

Understanding the principles of pattern formation driven by Notch signaling by integrating experiments and theoretical models
Federico Bocci, Jose Nelson Onuchic, Mohit Kumar Jolly

 

Modeling cell crawling strategies with a bistable model: From amoeboid to fan-shaped cell motion
E. Moreno, S. Flemming, F. Font, M. Holschneider, C. Beta, S. Alonso

 

Jamming and force distribution in growing epithelial tissue
Pranav Madhikar, Jan Åström, Björn Baumeier, Mikko Karttunen

 

Entropy-driven cell-decision making predicts fluid-to-solid transition in multicellular systems
Arnab Barua, Simon Syga, Pietro Mascheroni, Nikos Kavallaris, Michael Meyer-Hermann, Andreas Deutsch, Haralampos Hatzikirou

 

 

 

Tools & resources

 

 

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
Helena García-Castro, Nathan J Kenny, Patricia Álvarez-Campos, Vincent Mason, Anna Schönauer, Victoria A. Sleight, Jakke Neiro, Aziz Aboobaker, Jon Permanyer, Marta Iglesias, Manuel Irimia, Arnau Sebé-Pedrós, View ORCID ProfileJordi Solana

 

Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editing
Guinevere Ashley, Tam Duong, Max T . Levenson, Michael A. Q. Martinez, Jonathan D. Hibshman, Hannah N. Saeger, Ryan Doonan, Nicholas J. Palmisano, Raquel Martinez-Mendez, Brittany Davidson, Wan Zhang, James M. Ragle, Taylor N. Medwig-Kinney, Sydney S. Sirota, Bob Goldstein, David Q. Matus, Daniel J. Dickinson, David J. Reiner, Jordan D. Ward

 

Rapid self-selecting and clone free integration of transgenes into engineered CRISPR safe harbor locations in Caenorhabditis elegans
Zachary Christopher Stevenson, Megan J. Moerdyk-Schauwecker, Brennen Jamison, Patrick C. Phillips

 

 

An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches
Geoffrey W Meissner, Zachary Dorman, Aljoscha Nern, Kaitlyn Forster, Theresa Gibney, Jennifer Jeter, Lauren Johnson, Yisheng He, Kelley Lee, Brian Melton, Brianna Yarbrough, Jody Clements, Cristian Goina, Hideo Otsuna, Konrad Rokicki, Robert R Svirskas, Yoshinori Aso, Gwyneth M Card, Barry J Dickson, Erica Ehrhardt, Jens Goldammer, Masayoshi Ito, Wyatt Korff, Ryo Minegishi, Shigehiro Namiki, Gerald M Rubin, Gabriella Sterne, Tanya Wolff, Oz Malkesman, FlyLight Project Team

 

The Incubot: A 3D Printer-Based Microscope for Long-Term Live Cell Imaging within a Tissue Culture Incubator
George O. T. Merces, Conor Kennedy, Blanca Lenoci, Emmanuel G. Reynaud, Niamh Burke, Mark Pickering

 

Neural Plate Targeting by in Utero NanoinjEction (NEPTUNE) reveals a role for Sptbn2 in neurulation and abdominal wall closure
Katrin Mangold, Jan Masek, Jingyan He, Urban Lendahl, Elaine Fuchs, Emma R Andersson

 

Adaptable and Efficient Genome Editing by sgRNA-Cas9 Protein Co-injection into Drosophila
Kevin G. Nyberg, Joe Nguyen, Yong-Jae Kwon, Shelby Blythe, Greg J. Beitel, Richard W. Carthew

 

Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster
M. Alessandra Vigano, Clara-Maria Ell, Manuela MM Kustermann, Gustavo Aguilar, Shinya Matsuda, Ning Zhao, Timothy J Stasevich, George Pyrowolakis, Markus Affolter

 

Organoid Easytag: an efficient workflow for gene targeting in human organoids
Dawei Sun, Lewis D. Evans, Emma L. Rawlins

 

Illuminating chromatin compaction in live cells and fixed tissues using SiR-DNA fluorescence lifetime
Colin Hockings, Chetan Poudel, Kevin A. Feeney, Clara L. Novo, Mehdi S. Hamouda, Ioanna Mela, David Fernandez-Antoran, Pedro P. Vallejo-Ramirez, Peter J. Rugg-Gunn, Kevin Chalut, Clemens F. Kaminski, Gabriele Kaminski-Schierle

 

A Homology Independent Sequence Replacement Strategy in Human Cells Using a CRISPR Nuclease
Eric Wcislo Danner, Mikhail Lebedin, Kathrin de la Rosa, Ralf Kuehn

 

An improved CRISPR/dCas9 interference tool for neuronal gene suppression
Corey G. Duke, Svitlana V. Bach, Jasmin S. Revanna, Faraz A. Sultan, Nicholas T. Southern, M. Natalie Davis, Nancy V.N. Gallus, Allison J. Bauman, Robert A. Phillips III, Jeremy J Day

 

CellExplorer: a graphical user interface and standardized pipeline for visualizing and characterizing single neuron features
Peter Christian Petersen, György Buzsáki

 

Tethering Piezo channels to the actin cytoskeleton for mechanogating via the E-cadherin-β-catenin mechanotransduction complex
Jing Wang, Jinghui Jiang, Xuzhong Yang, Li Wang, Bailong Xiao

 

Multifocal imaging for precise, label-free tracking of fast biological processes in 3D
Jan N. Hansen, An Gong, Dagmar Wachten, Rene Pascal, Alex Turpin, Jan F. Jikeli, U. Benjamin Kaupp, Luis Alvarez

 

In utero gene transfer system for embryos before neural tube closure reveals a role for Hmga2 in the onset of neurogenesis
Naohiro Kuwayama, Yusuke Kishi, Yurie Maeda, Yurie Nishiumi, Yutaka Suzuki, Haruhiko Koseki, Yusuke Hirabayashi, Yukiko Gotoh

 

Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork
Matthew Wincott, Andrew Jefferson, Ian M. Dobbie, Martin J. Booth, Ilan Davis, Richard M. Parton

 

Integration of multiple imaging platforms to uncover cardiac defects in adult zebrafish
Anabela Bensimon-Brito, Giulia L. M. Boezio, João Cardeira-da-Silva, Astrid Wietelmann, Christian S. M. Helker, Radhan Ramadass, Janett Piesker, Arno Nauerth, Clemens Mueller, Didier Y. R. Stainier

 

Plant protein-based diets can replace a fish meal-based diet for sustainable growth and body composition of zebrafish
Toluwalase Anthony Aiyelari, Abdul Shakoor Chaudhry

 

Slc26a9P2ACre, a new CRE driver to regulate gene expression in the otic placode lineage and other FGFR2b-dependent epithelia
Lisa D Urness, Xiaofen Wang, Chaoying Li, Rolen M Quadros, Donald W Harms, Channabasavaiah B Gurumurthy, Suzanne L Mansour

 

 

 

Research practice & education

 

Preprinting a pandemic: the role of preprints in the COVID-19 pandemic
Nicholas Fraser, Liam Brierley, Gautam Dey, Jessica K Polka, Máté Pálfy, Jonathon Alexis Coates

 

A Multi-perspective Analysis of Retractions in Life Sciences
Bhumika Bhatt

 

Working groups, gender and publication impact of Canada’s ecology and evolution faculty
Qian Wei, François Lachapelle, Sylvia Fuller, Catherine Corrigall-Brown, Diane S Srivastava

 

The representation of women as authors of submissions to ecology journals during the COVID-19 pandemic
Charles W Fox

 

 

 

 

 

(No Ratings Yet)
Loading...

Today we return our interest to human development, focusing on a special blood cell: the macrophage. Produced in multiple, stem cell-independent waves, macrophages colonize the developing foetus early on, forming several tissue-resident populations. This includes the microglia which are essential for brain and spinal cord development. In this paper, the authors looked into macrophage development in the human embryo, drawing parallels to the better-known mouse and zebrafish models.

First of all, they performed single-cell RNA sequencing on blood cells sampled from 8 human embryos across different Carnegie stages (11 to 23). They sampled the yolk sac (where the first macrophage wave arose), head, liver, blood, skin, and lungs; all sites successively colonized by macrophages. The first round of sequencing was performed with STRT-seq and analysed 1231 cells, from which 15 populations could be identified. This included a yolk-sac derived progenitor group (YSMPs) that strongly resembled the established signature for mouse multipotent cells called erythro-myeloid progenitors (EMPs). Notably, YSMPs were almost completely biased toward the myeloid cell fate, as confirmed by in vitro studies. The second round of sequencing using 10x Genomics confirmed the previous results in more than 11,000 cells. The combined STRT-seq and 10x data were used to define developmental trajectories, in order to understand the origin of the tissue-resident macrophage populations. Interestingly, several of these populations seemed to have already initiated their tissue residency genetic programs, as has been observed in the mouse. Although not a lineage tracing study, the authors described a major contribution of yolk sac-derived macrophages to microglia development. Conversely, YSMPs seem to play a secondary role in microglia formation, a result consistent with mouse development.

In summary, this work confirms the high degree of conservation between species, creating a roadmap for macrophage differentiation. Moreover, it is a testament to the maturity of the single-cell transcriptomic field and the accompanying data analysis.

 

Bian et al. “Deciphering human macrophage development at single-cell resolution”

Doi: https://doi.org/10.1038/s41586-020-2316-7

(2 votes)
Loading...

Team “Environment-Metabolism-Spermatogenesis Pathophysiology-&-Inheritance»

Institute Genetic, Reproduction & Development,Inserm U1103, CNRS UMR 6293, Université Clermont Auvergne.

28 place Henri Dunant, 63001 Clermont-Ferrand Cedex, France

https://www.gred-clermont.fr/directory/team/en/team-13-environment-spermatogenesis-pathophysiology-and-inheritance/

Duration: 2 to 3 years

Starting date: To define due to international confinement.
Contact: david.volle@inserm.fr or david.volle@uca.fr

Our lab studies the mechanisms that lead to testicular pathophysiologies such as fertility disorders or testicular germ cell cancers. We are interested in deciphering the impacts of altered metabolism and/or of exposures to environmental molecules on testicular physiology. In order to perform such work, we are using pharmacological approaches combined with specific genetic models such as C. elegans, transgenic mice, or culture cell of tumor cell lines.

The background of the project. The incidence of testicular germ cell tumors (TGCT) has increased in the last decades. TGCT are the most common solid cancers in young adults. Moreover, 10 to 20% of patients have forms that are resistant to treatment. It is thus essential to improve the treatments in order to provide better care to people with cancers that are resistant to current treatments. In addition, patients who received chemotherapy or radiotherapy are at higher risk of developing a second malignant tumor. To answer the question of treatment efficiency, there is a need to better understand the etiology of TGCT, which remains poorly known.
In order to explore the questions of TGCT biology and their sensitivity to chemo-drugs, we have started a new field of research in our team focusing on nuclear receptors, which has been associated with the development of cancers.

Description of the project. To achieve this project, we will use genetically modified mice that are predisposed to TGCT and testicular organotypic culture system. In addition, we will develop single-cell approach to decipher the molecular mechanisms involved in tumor development, aggressiveness, or chemo-resistance. This analysis will be key to study switches of homeostasis and metabolism between normal to tumor cells. Through these models combined with high-throughput approaches (such as RNAseq), candidate will analyze the biology of germ cell tumors (initiation, progression, and invasion) as well as their sensitivity to therapy in order to decipher the roles of targeted signaling pathways. In addition, the candidate will use C. elegans as a powerful genetic model to validate candidates defined in mouse models. This transposition will be useful to develop a new model to study germ cell tumors in regards to the 3R ethical rules.
Expected results. The validation of these models will allow us to first extend their use in the context of TGCT biology in order to provide mechanistic connections between selected signaling pathways and TGCT etiology. Secondly, this work should provide new insights for providing novel prognostic markers and potential therapeutic targets.

Candidates. Qualified candidates should be self-driven and highly motivated individuals with an established track record of success, including first-author publications. Experience in cancer biology, developmental biology, reproductive biology, cell, and molecular biology, or related field(s) is desirable. The candidate must have experience in genetically modified mouse models and/or C. elegans biology, cell culture, single-cell and molecular biology techniques (RNAseq, etc.), bioinformatics skills.

For prompt consideration, please email the following items to Dr. David VOLLE: david.volle@inserm.fr
*A one-page cover letter describing areas of research interests and career goals
*Curriculum vitae with bibliography
*Contact information for 3 references

(No Ratings Yet)
Loading...