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Behind the paper: how to turn scales into feathers 

Posted by , on 16 June 2023

A recent paper in Science Advances entitled ‘Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo’ reports an experimental method for transforming chicken scales into true feathers (1). We caught up with Dr. Rory Cooper, the first author of the article, to find out more about the story behind the paper.

A transient change in expression of one gene (Shh) can produce a cascade of developmental events leading to the formation of feathers instead of scales. © UNIGE / Cooper & Milinkovitch

How did you get started on this project?

I became interested in the evolution and development of skin appendages (such as scales, spines and feathers) during my PhD with Dr. Gareth Fraser, when I was studying the patterning of scales and teeth in the shark. I wanted to continue working in this field, and luckily the LANE (Milinkovitch-Tzika lab) advertised a postdoc position at just the right time. This lab is renowned for studying skin appendage development in really diverse non-classical models such as snakes, lizards and exotic mammals, so it was the perfect destination for me. One key finding by Michel Milinkovitch & his then post-doc Nicolas Di-Poï, was that not only hair and feathers, but also reptilian scales, develop from placodes – localised thickenings of the skin that constitute the foundations of most skin appendages (2). When I arrived in the LANE in 2020, there was already an ongoing project investigating chicken scale development, so it was easy for me to get started straight away.   

What was already known about ectopic feather induction in chicken embryos? 

There have been a few published articles reporting a scale-to-feather conversion in the chicken embryo (3-5). However, the results of these studies are variable. First, not all of these scale-to-feather conversions result in true, regenerative feathers, second, the coverage of these ectopic feathers was not ubiquitous, and third, the post-embryonic development of these feathers had not been examined. Also, our lab had recently developed a really nice protocol for injecting precise quantities of drugs directly into the veins of developing chicken embryos (as well as other hard-shelled eggs) (6). You can check this out in the video below. This project provided a great opportunity to try out this method.

Intravenous injection protocol for treating hard-shelled amniote eggs (Cooper et al. 2023)

Can you summarise your key findings?

We investigated the role of the sonic hedgehog (Shh) pathway in mediating skin appendage fate in the chicken. This pathway is essential for loads of developmental events, and is really important in controlling the early development of placodes. We injected a precise dose of a Shh pathway activator directly into the blood stream of developing embryos (6), at the exact time that scales begin to appear on their footpads. We then collected the embryos at various stages after injection, and even allowed some to hatch so that we could examine the effect in adult chickens. Incredibly, every single chickens injected with the Shh activator had abundant ectopic feathers covering their feet. We tracked the development of these ectopic feathers in hatched chickens, and saw that they transitioned from juvenile down-type feathers into true contour feathers observed in adult birds. This confirmed that our experimentally-induced feathers are true, regenerative feathers.  


Movie showing a 3D reconstruction of light-sheet fluorescence microscopy imaging showing experimentally induced ectopic feather buds in the embryonic chicken © UNIGE / Cooper, Megahed & Milinkovitch

When doing the research, did you have any particular result or eureka moment that has stuck with you?

I distinctly remember looking at the first treated sample under the microscope – it was collected after 4 days, meaning that the ectopic feather buds were still relatively undeveloped. At first glance it looked like an incredibly hairy chicken foot. I showed the sample to my boss, and he suggested that we should see what happened if we allowed the embryos to develop until hatching. A few weeks later we had our first feather-footed hatchling chickens! 

And what about the flipside: any moments of frustration or despair?

Whilst attempting to harvest samples for RNAseq, the treatment suddenly began to kill all of the embryos. As there was a short power outage in our lab during the incubation of the eggs, I initially assumed that the embryos were slightly less developed than before. I then repeated the experiment with fresh eggs, and exactly the same thing happened. Finally, I contacted the farmer who provides the eggs, and he told us that they had just replaced their laying hens, meaning that the eggs were now coming from much younger chickens, which lay much smaller eggs. After adjusting the treatment to account for this change, the experiment worked perfectly.   

What is next for you after this paper?

I’m working on some more really exciting skin appendage-related projects in the Milinkovitch-Tzika lab. We have an additional manuscript on chicken scale development in the pipeline, and Michel has had me heavily involved me in another project on crocodile scale patterning – they’re both really cool stories, and hopefully we will be able to share them with you soon!

References

1. R. L. Cooper, M. C. Milinkovitch, Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo. Science Advances 9, eadg9619 (2023).

2. N. Di-Poï, M. C. Milinkovitch, The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes. Science Advances 2, e1600708 (2016).

3. D. Dhouailly, M. H. Hardy, P. Sengel, Formation of feathers on chick foot scales: A stage-dependent morphogenetic response to retinoic acid. Journal of embryology and experimental morphology 58, 63-78 (1980).

4. R. B. Widelitz, T. X. Jiang, J. Lu, C. M. Chuong, Beta-catenin in epithelial morphogenesis: conversion of part of avian foot scales into feather buds with a mutated beta-catenin. Dev Biol 219, 98-114 (2000).

5. P. Wu et al., Multiple Regulatory Modules Are Required for Scale-to-Feather Conversion. Mol Biol Evol 35, 417-430 (2018).

6. R. L. Cooper, G. Santos-Duran, M. C. Milinkovitch, Protocol for the rapid intravenous in ovo injection of developing amniote embryos. STAR Protocols 4, 102324 (2023).

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Postdoctoral Position in Stem Cell Biology

Posted by , on 15 June 2023

Closing Date: 1 January 2024

A postdoctoral opportunity is available in The Department of Biomolecular Engineering, Baskin School of Engineering at University of California, Santa Cruz (UCSC). The department has a vibrant research community with strong focus on stem cell biology, RNA biology and Genomics.  Our lab is also affiliated with The Institute for the Biology of Stem Cells and the Genomics Institute.

How stem cells decide to choose between two conflicting fates, division vs. differentiation, is an unsolved mystery of stem cell biology. The overarching goal of Shariati lab is to determine the mechanisms that link cell division to cellular differentiation in pluripotent cells. We will combine emerging genome-editing technologies with single cell imaging to determine regulatory principles of cell fate decisions in pluripotent cells.

Applicants with demonstrated experience in any of the following areas are highly encouraged to apply: Stem Cell BiologyTranscription, Quantitative Single Cell imaging, CRISPR screening and Mouse Genetics. The potential start date is Fall 2023.

As your postdoc advisor, I will support you to reach the career goals that you set for yourself. In addition, UCSC has exceptional career development programs for postdoctoral fellows. Santa Cruz is a great town for postdocs as you can easily refresh your mind by strolling in beautiful redwood forests on campus or going to one of the many nearby beaches. Please email me (alish {a} ucsc.edu) a cover letter stating your scientific background and interest, your CV and names/addresses of your advisors.

Applicants from underrepresented groups in sciences are highly encouraged to apply. Informal inquires also are welcomed!  

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Moving towards heart success – Disease Models & Mechanisms Special Issue

Posted by , on 15 June 2023

Heart failure occurs when the heart is weakened or damaged, or when there is a problem with the heart’s valves or rhythm. Strikingly, it is the leading cause of hospitalisation and deaths in men and women worldwide. Nevertheless, significant gaps remain in our understanding of heart failure, and treatment, at best, results in disease remission. Our goal in shaping this Special Issue of Disease Models & Mechanisms (DMM) was to compile original Research, Resource and Review-type articles that investigate the genetic and biological mechanisms of heart failure and identify potential therapeutic strategies, as summarised in an Editorial from Guest Editors Jeroen, Milena Bellin and Ravi Karra.

Guest Editors Jeroen Bakkers, Milena Bellin and Ravi Karra

A significant risk factor for heart failure is congenital heart defects, which are structural abnormalities affecting 8 out of every 1000 new-borns. The causes of congenital heart defects are diverse, which encourages the exploration of these disorders in the context of cardiac development. A Review from Christian Mosimann’s group summarises how defects in lateral plate mesoderm development drive congenital defects of multiple organs, including the heart, and a Research article from Didier Stainier and colleagues uses zebrafish to study the role of the epicardium, the outermost layer of the heart, in coordinating growth of the myocardium. Sally Dunwoodie and colleagues also published a study on a metabolic disease that results in congenital heart malformation.

Beyond disordered heart development, arrhythmias, which occur due to disruptions in the heart’s electrical system, represent another risk factor for heart failure. A Review by Vincent Christoffels and colleagues, published in this issue, explores the genetic causes of heart arrhythmias. Another Review from Eva Van Rooij’s group discusses the use of precise genome editing to generate models of and to potentially treat cardiomyopathies, which involve the remodelling of heart muscle and can also lead to heart failure if left untreated.

This special issue also provides an insight into the current understanding of acquired heart disease, with studies ranging from the investigation of strategies to mitigate cardiac remodelling upon injury to how the gut microbiome metabolite trimethylamine-N-oxide affects heart function.

Finally, the Special Issue includes ‘A Model for Life’ interview between leading experts Jeroen Bakkers and Didier Stainier, who discuss the advantages and disadvantages of zebrafish models of heart disease. Model organisms, both large and small, have been instrumental in advancing this field, with articles in this Special Issue using or discussing the use of mice, rats and pigs as valuable models of heart failure with strong translational potential. In addition, in vitro models, such as organoids, will provide new research opportunities to study cardiac development and related diseases, as summarised in the beautiful At a Glance poster article by Lika Drakhlis and Robert Zweigerdt and in ‘A Model for Life’ interview with Professor Christine Mummery. The Research, Resource and Review-type articles in this Special Issue cover a wealth of seemingly diverse topics. However, preventing, understanding and treating heart failure are the common threads that bind these articles. Follow the link below to read all the articles in this Special Issue for free.

https://journals.biologists.com/dmm/issue/16/5

Cover of Disease Models & Mechanisms Special Issue.
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Genetics Unzipped: Da Vinci’s DNA: What happens when genetics meets art

Posted by , on 15 June 2023

“In an attempt to fight modern-day art forgery, researchers have been developing techniques that involve using DNA to tag pieces of art”

Dr Kat Arney

In the latest episode of the Genetics Unzipped podcast, we’re taking a journey into the world of art and artefacts, extracting DNA from paintings, hair and even chewing gum, and unearthing the genetic secrets of long-dead legends like Da Vinci, Van Gogh and Beethoven.

Genetics Unzipped is the podcast from The Genetics Society. Full transcript, links and references available online at GeneticsUnzipped.com.

Subscribe from Apple podcasts, Spotify, or wherever you get your podcasts.

Head over to GeneticsUnzipped.com to catch up on our extensive back catalogue.If you enjoy the show, please do rate and review on Apple podcasts and help to spread the word on social media. And you can always send feedback and suggestions for future episodes and guests to podcast@geneticsunzipped.com Follow us on Twitter – @geneticsunzip

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Development presents… August webinar

Posted by , on 14 June 2023

Our August webinar will be chaired by Development Editor Cassandra Extavour (Harvard University) and features three early-career researchers studying evo-devo. The webinar will be held using Zoom with a Q&A session after each talk.

Wednesday 2 August 2023 – 15:00 BST

Hannah Gruner (Swarthmore College)
‘Revealing the mechanisms of neural-mediated cardiac proliferation in Ciona robusta

Seth Donoughe (University of Chicago)
‘Uncovering evolutionary patterns of epithelial packing, ECM structure, and morphogenesis’

Allan Carrillo-Baltodano (Queen Mary University)
‘Ancestral synergy of TGF-beta and MAPK during dorsal-ventral specification in Bilateria’

To see the other webinars scheduled in our series, and to catch up on previous talks, please visit: thenode.biologists.com/devpres

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Meeting report- 2023 Young Embryologist Network Conference 

Posted by , on 12 June 2023

By Ioakeim (Makis) Ampartzidis, Courtney Lancaster, Danielle Liptrot, and Rosie Marshall

Happy Birthday YEN! 

The Young Embryologist Network (YEN) turns 15 this year. YEN serves as a vibrant platform for knowledge exchange and collaboration, with a particular emphasis on showcasing the work of early career researchers (ECRs — postdocs and PhD students). It has been 15 years full of inspiring science and what better way to celebrate than with a debrief of this year’s fantastic conference: 

Hot Off the Press: Scientific Talks

Pre- and peri-implantation development was a particular focus in the scientific talks this year, highlighting the drive to improve in vitro embryo work relating to fertility. Two of the invited speakers presented impressive work on the subject: Katsuhiko Hayashi from Osaka University kicked off the day with an impressive overview of his ground-breaking work on reconstructing oogenesis from pluripotent stem cells, which he hopes will be used to save the endangered Northern White Rhino (only two females of this species remain); Andrea Pauli from the Institute of Molecular Pathology in Austria later showed us her fascinating work on how closely related species prevent interspecies fertilisation (in the case of zebra- and medaka-fish, it’s just one protein expressed on the egg!)  

Many of the selected talks also focused on these early stages of development: Chloe He showed us how deep learning and non-invasive imaging of cleavage-stage human embryos can help us to study how cell arrangement affects blastocyst quality; Johanna Gasler demonstrated the requirement of orphan nuclear receptors on early development and zygotic genome activation in murine embryos; Lessley Sepulveda-Rincon produced apoptosis-deficient primordial germ cells in mouse chimeras to understand their functional incorporation to the mouse embryo; Zukai Liu presented the specification of extra-embryonic mesenchyme in primate development, at a genome level revealing ape-specific attributes.  

Andrea Pauli presenting at the 2023 YEN Conference
Andrea Pauli’s exciting talk on fertilization.

Once again, YEN showcased the wide range of fascinating organisms used in developmental biology research. Michael Emmerson revealed how zebra finches can detect sound cues to alter their growth rate in utero (including expert impressions of zebra finch heat calls)! The utility of the old favourite Drosophila was once again on show, with Gloria Jansen’s impressive talk on how paternally inherited transposable elements alter penetrance of germ cell loss. O. fusiformis, a bilaterally symmetrical organism, is another neat model on which Allan Carrillo-Baltodano studies dorso-ventral patterning. Finally, zebrafish once again lived up to its reputation as a beautiful model for imaging, with Agatha Ribeiro da Silva utilising the model to study endothelial cell migration defects in heart development. 

Wrapping up the short talks was the final invited speaker, Laura Pellegrini. She is a current postdoc in Madeline Lancaster’s lab who is soon moving to King’s College London to start her own group. She showed us how choroid plexus organoids have a wide range of applications, from a drug screening system to test whether drugs can cross the blood-CSF barrier, to a model for studying viral infection of the brain. Certainly ground-breaking work and we look forward to seeing how her group progresses! 

Sammy Lee Memorial Lecture

The final talk of the day was the Sammy Lee Memorial Lecture, which was established to remember Sammy and to reflect on his contributions to developmental biology and his passion for inspiring the next generation of scientists. 

Dr Sammy Lee and the memorial Sammy Lee medal designed by Felicity Powell
Dr Sammy Lee and the memorial Sammy Lee medal designed by Felicity Powell.

Sammy was a visiting professor in Cell and Developmental Biology at University College London (UCL), whose life-changing work was responsible for outstanding advancements in reproductive health and fertility. Sammy started his scientific journey with a PhD at UCL, where he was working on nerve muscle interaction. Not long afterwards, Sammy became interested in developmental biology. This led to his work on gap junctions in mammalian embryos where he discovered a link between gap junction communication and early embryonic development. He then became a clinical embryologist in 1985, helping to perform some of the UKs first egg donations, before being appointed as the scientific director of the Wellington IVF department. Sammy’s team was the first in the UK to perform gamete intrafallopian transfer (GIFT), pioneered a method of mechanical assisted hatching in the UK to improve chances of in utero implantation and produced the world’s first virus free ICSI (intra-cytoplasmic sperm injection) baby to a HIV discordant couple.  

Fitting with Sammy’s character, the Sammy Lee Memorial Lecture was delivered by Henrik Kaessmann who entertained us with the wonders of sex chromosomes. He drew our attention to the small size of the Y chromosome which has a striking feature of chromosome wide gene decay. He then discussed gene dosage across different evolutionary lineages from mammals to birds.

It was Sammy’s wish to present a medal to a young developmental biologist on the merit of scientific communication and outstanding research. The 2023 Sammy Lee medal for best short talk was awarded to Clara Munger, who impressed the judges with her presentation about marmoset amnion specification. She told us about her work using marmoset embryonic stem cells to make spheroids in a microgel culture system, and how this system can be used to interrogate signalling pathways required for lineage specification in the post-implantation embryo. The runner-up went to a fantastic talk from Ashley Libby who discussed progenitor cell dynamics and gene regulatory networks during neural tube development.

Poster Sessions

Conference attendees discussing during poster session at 2023 YEN conference.
First poster session of the day.

The day was split up by two fantastic poster sessions in the morning and afternoon, with over 30 posters being presented both in person and online. The topics ranged from sea urchin larval skeletogenesis to the identification of unwanted genetic variants in stem cell populations, having implications in evolution, developmental biology and regenerative medicine. In addition, it was excellent to see projects being presented from a range of early career researchers, from master’s students to senior postdocs. The diversity in presenters and topics incited long discussions in both poster sessions, making it difficult to coax everyone back to their seats. It will certainly be exciting to see what collaborations were spawned in these sessions, and how it will influence the direction of developmental research in years to come. 

With all the excellent projects being presented, it was surely a hard decision for the judges to award the poster prize and runner up. That being said, on observing the work of prize winner – Francisco Manuel Martin-Zamora – and runner up – Adiyant Lamba – it is evident their projects are both deserving of recognition. Francisco, a PhD student at Queen Mary University, presented his work on how epigenetics may underpin temporal shifts in the diversification of larval and bilaterian life cycles. Adiyant, also a PhD student based at the University of Cambridge, described the heterogeneities which influence early cell fate decisions between the inner cell mass and the trophoectoderm. The future of these two young embryologists is very bright! 

Perspectives: EDI Awareness in Academia 

A refreshing addition to the science-rich day was the addition of scientific perspective sessions, introduced by the committee in 2022. This year, the focus was on Equity, Diversity, and Inclusion (EDI) in science, raising awareness for the importance of embracing EDI in scientific meetings.  

This year’s perspective experts Alison Forbes, Head of Inclusion at The Francis Crick Institute in London, UK, and Rafael Galupa, a social entrepreneur and group leader at the Centre for Integrative Biology in Toulouse, France, shared their valuable perspectives on the topic. They focussed on the current state of underrepresentation in science and offered practical strategies to enhance inclusivity. When asked how ECRs can realistically improve diversity, Alison suggested to “[…] start with self-education. Read some books and listen to podcasts;” while Rafael encouraged us all to “[…] find a way to contribute that is aligned with their personality and availability”. By prioritizing EDI in scientific meetings, we collectively advance knowledge and ensure that the science we all love and care about reflects the full spectrum of human perspectives and capabilities. For a more in-depth conversation on this topic, read this interview with Alison and Rafael. 

The faces behind YEN 2023

A big shout-out to the organising committee of YEN Conference 2023, for their hard work, fresh ideas, and organising skills. This year’s chairs Foteini and Jeremie along with the fantastic team of Michelle, Sergio, Jack, Ollie, Luca, Ferran, Olivia, Oliver, Matyas, Jesus, Mint, Claudia, Irina, and Christos, celebrated the 15th birthday of Young Embryology Network with a memorable Conference. The team is composed mainly from PhD students and ECRs based in London and if you like to learn more about their journey follow them on twitter @YEN_community.

Group photo of Young Embryology Network Committee 2023
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Interview with the #YEN2023 panelists on equity, diversity and inclusion in science

Posted by , on 12 June 2023

The Young Embryologist Network (YEN) Conference was held in May at the Francis Crick Institute. This year, apart from the scientific talks, the programme included a panel discussion on ‘Equality, diversity and inclusion in science’. We caught up with the two panelists, Alison Forbes (Head of EDI, the Francis Crick Institute), and Rafael Galupa (Scientist & social entrepreneur), to talk more in-depth about this important topic.

How did you find the YEN2023 meeting?

Alison: I loved it, great fun to speak to a large audience of early career scientists. I enjoy speaking to scientists as an ‘outsider’ (I studied humanities, I’m in HR, I am not a researcher). It means I can bring a fresh eye to science as an industry, and I think the audience enjoyed that.

Rafael: As a scientist, I really enjoyed myself and was very impressed and excited by all the science presented! This is a great meeting for me to attend regularly in the future and where to send my future lab members. As a scientist concerned with EDI issues, I thought it was great that the organisers included three different moments in the scientific programme to discuss such topics.

Could you tell us more about your background? What made you decide to be involved with EDI initiatives?

Alison: I grew up in Hackney, London and went to a north London grammar school, then a Russell Group university.

I am from a middle-class, academic family. But I always found snobbery, elitism and other ‘-isms’ irritating, which is why I am drawn to this work. I remember my grammar school teachers repeatedly telling us that we were ‘the best of the best’. That attitude never sat right with me.

I worked at UCL for 12 years in widening participation, working on social class and diversity in undergraduate admissions. I ran long term programmes for highly able teenagers from London state schools. They were mostly from low-income backgrounds, lots of refugee or immigrant families, usually parents had not been to university. These students were intelligent, sharp and witty, asked challenging questions, and were tough and resilient.

They were highly ambitious, as were their parents. I learned a lot from these young people, and got to see ‘my kids’ progress to UCL or other excellent universities. I also worked with UCL undergraduates, and saw how working class or minoritised students struggled with imposter syndrome. I remember young people telling me they had nowhere quiet to study, that they had had 4 teachers for Maths A level in one school year, or how their parents pressured them to apply for medicine and would not let them consider any other subjects.

It gave me a better understanding of how class, social mobility and privilege affect careers, and how oblivious we can be of own privilege (‘Well, I worked hard to get here!’).

I moved into EDI through a secondment at UCL. It is a fascinating and evolving field. There is much to be done, and you can have a real impact. I’ve been at the Crick since November 2021, and I love it.

Rafael: I grew up in the suburbs of Lisbon, surrounded by a big extended family. Because my dad went to university and became a power engineer, my household lived comfortably, but this wasn’t the case for the others in the family, or for the households of my school friends. I grew up side by side with these inequalities, and perhaps that’s why I’ve always felt a responsibility to address them.

I started volunteering when I was sixteen, in an orphanage – those kids had a completely different social structure around them (compared to mine), but not necessarily less supportive; this made me understand the importance of a support network, whichever nature it has, and how it is possible to build one if we don’t have the privilege to have one more “naturally”.

Later, in the University of Lisbon, while studying Molecular Biology and Genetics, I was part of a support group for students with disabilities. I had a weekly studying session with a younger undergraduate who was losing his vision. This was a major challenge for him, aggravated by how the classes, the studying materials, basically everything!, were very poorly accessible.

During my PhD, I came across Native Scientists, a nonprofit organisation that connects students with underserved children via interactive workshops, and to this day I have been involved in such activities. During my postdoc I participated in the project Letters to a Prescientist, which promotes letter exchanges between scientists worldwide and students at US-based schools where at least 60% of students qualify for “Free or Reduced Price Lunch”.

This experience inspired me and a friend to start a similar organisation, Cartas com Ciência, targeting students from low-income communities in Portuguese-speaking countries. Many studies show how students from low-income communities are less likely to pursue university studies and choose STEM careers, which promotes the perpetuation of the low-income cycle. The reasons for such stats are many, but include the lack of science role models around those students and often not knowing what a scientific career is. This is something that we as scientists can help eradicate! And there are many other levels at which we can act. So how not to get involved with initiatives that promote such fundamental values as equality, diversity and inclusion?

What do you think is the current biggest challenge in achieving an equal, diverse, and inclusive research culture?

Alison: There isn’t one single challenge, and there isn’t one single solution. It is complex.

Until we have urgency on these issues within government, and within science leadership, progress may well remain slow.

When something is urgent, we make it happen. For example, the sudden pivot by many employers to remote working during COVID. Home working was something disabled employees had asked for before COVID to help manage their conditions, but because allowing remote working was not the norm and was not urgent, it wasn’t seen as possible.

Many organisations are risk averse. They don’t want to be ‘the first’ to do something– they want to their peers to go first, so they can get assurance that an intervention works, and will not cause reputational damage. For example, after some Russell Group universities piloted contextual undergraduate admissions, it became common practise. Similar for interventions like reverse mentoring or various positive action schemes. So, the organisations that do ‘go first’ and take that risk should be credited.

Rafael: As Alison stated, there isn’t one biggest challenge. We all need to change our mindset, but especially people at the higher management of our research institutions. We need a more inclusive, healthier, people-centric work culture. I am sure that things will change – how fast, it remains to be seen. Planck’s principle basically says that change does not occur because individuals change their minds but rather because successive generations have different views (or in more crude terms, “one funeral at a time”). This will certainly be the case, but I also believe in the power of education, and so training in inclusive leadership, for instance, might accelerate things, as a good friend working in EDI, Roshni Mooneeram, taught me. We also need funding agencies and governments to take the lead and set the “tone” – change at those levels is the most likely to have a domino effect. Also, something that is at the root of many of the problems in research institutes (and in society in general) is the frenetic pace at which we live and work – complex problems, such as EDI issues but also most of the scientific questions we address, need us to pause more frequently, to think and reflect.

Why is it important to raise awareness on EDI in scientific meetings?

Alison: Scientists need to understand equity, diversity and inclusion for several reasons:

  • Equity principles should inform research design. Scientists should be considering sex as a biological variable, the ethnic diversity of patients in clinical trials, and how to design equitable collaborations with scientist colleagues in lower income countries.
  • Scientists should be recruited by an evidence-based, equitable, transparent process. When a PI recruits a post doc, do they use a process designed to minimise bias and to attract diverse candidates? For example, do they name blind applications, use standard interview questions that are scored, and do they have gender and if possible ethnicity mix on interview panels? There is a lot of evidence that diverse teams are more high performing, but often common recruitment practises in science penalise minoritized applicants.
  • Most scientists agree public communication is important. But if scientists do not have a nuanced understanding of ‘how they come across’ when speaking to diverse audiences, it can harm public health messages. In the pandemic we saw high vaccine hesitancy in communities of colour in the UK and USA, and among some patient communities with chronic conditions. This was partly because of low trust due to prior experiences of racism, or histories of medical trauma. So, scientists have to understand those contexts when they speak to different audiences.

Rafael: Scientific meetings provide a great platform to discuss EDI because on the one hand, we can reach more people at once; on the other hand, everyone paused their everyday work and is in a new environment, which is more conducive for reflexion and thinking about issues that most of us do not normally think about. Moreover, such complex issues need collective thinking and collective actions, and scientific meetings definitely help with those.

What can early-career researchers do, both individually and as a community?

Alison: As an individual, start with self-education. Read some books and listen to podcasts; I’ve recommended some below.

Then interrogate your small daily actions and decisions. Do you ask questions about diversity in committee? For example, do you ask to see gender or ethnicity pay gap data, or recruitment data, or ask about what inclusion training your institute provides? Who do you defend, advocate for, or invite to join you at conferences? And who do you interrupt or ignore? Who do you seek to build relationships with?

As a wider community, you have power in numbers. Networks like the Node, the EDIS Group or grassroots organisations like Black in Cancer or BBSTEM have a big collective impact and should be nurtured.

Rafael: As Alison stated, join ongoing initiatives – they assume many different forms and flavours, so I’m sure that everyone can find a way to contribute that is aligned with their personality and availability. It’s also important to remember that we all have a certain degree of control over what happens around us (and to us as well) and certainly over how we do things. As we often say at Cartas com Ciência, EDI is about what we do but also how we do it and with whom we do it. Alison’s questions illustrate how we can incorporate such thinking in whichever actions or initiatives we think of doing, from setting a collective journal club, to organising a meeting or a public engagement activity.

Where can people find out more on this topic?

Alison: Nature ran a special issue about racism in science in October 2022. I also recommend their Nature careers: Working scientist podcast series. They often have episodes about inclusion, for example their mini-series Science diversified.

The EDIS Group (equality diversity and inclusion in science) have excellent resources and toolkits.

The book Invisible Women: exposing data bias in a world designed for men by journalist and campaigner Caroline Criado-Perez has some excellent chapters about research design. Female cells, animals, and patients are often under-studied in discovery research and clinical trials. This leads to outcomes like women being much more likely to be misdiagnosed for heart attacks, because they present with ‘atypical’ symptoms. Her book emphasises the importance of studying sex as a biological variable.

Another book I recommend often is The Class Ceiling: why it pays to be privileged by LSE academics Sam Friedman and Daniel Lauriston. They studied labour market data and found clear evidence of a pay gap between middle- and working-class employees in similar roles. They conducted anonymous interviews about experiences of class at work, with staff in a large TV company, an architecture firm and a financial firm. They don’t look specifically at science as an industry, but these themes are common across all sectors. A really insightful and entertaining read.

Rafael: The major scientific journals in life sciences have been regularly publishing special issues on EDI topics, for instance, Cell Press has collections on “Building inclusivity in science”, “Black in science”, “Women in science”, “LGBTQ+ in science” (here). Or the “Diversity, equity and inclusion in science” collection from Nature Human Behaviour (here). Hopefully all those articles are open access! Science published a short and comprehensive article on “How to begin building a culture of diversity, equity, and inclusion in your research group” (open access).

Two amazing books on how science has actually contributed to sexism and racism, I recommend Angela Saini’s books Inferior and Superior (respectively). They present and digest lots of interesting facts and studies, and Angela does a great job in always trying to compare and contrast different perspectives. Plus, it’s fun to read. On improving scientific culture in general, I recommend Uri Alon’s initiatives and available materials (here).

Want to find out what else happened at the YEN Conference? Read the detailed meeting report written by Ioakeim (Makis) Ampartzidis, Courtney Lancaster, Danielle Liptrot, and Rosie Marshall.

To learn more about YEN, follow them on twitter @YEN_community.

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Made the Same Way: A human egg looks nothing like a human

Posted by , on 9 June 2023

In the final episode of the Human Developmental Biology Initiative’s podcast, hip-hop artist Aubz meets Oxford University scientist Shankar Srinivas and they discuss questions such as what is human developmental biology and why is it important?

At the end of the episode, the pair will write and record an original piece of music inspired by their meeting, exploring science in a brand new way.

“We speak of science as one thing, and it’s not. It’s many different things.”

– Shankar Srinivas

About the participants

Shankar is Professor of Developmental Biology at the University of Oxford, at the Department of Physiology Anatomy and Genetics, in the Institute for Developmental and Regenerative Medicine. His group uses mouse and human embryos to study how the body is shaped, and how the heart forms and starts to beat. Shankar is also passionate about science outreach and public engagement. His group participates regularly in science festivals, and collaborates with dancers, choreographers and Virtual Reality specialists to generate movement based art, to explore different perspectives on how the form of the body is determined.

Aubz is a Manchester-based hiphop artist.

Please subscribe and listen to Made the Same Way on Apple podcasts, Spotify, or wherever you get your podcasts. If you enjoy the podcast, please rate and review us on Apple podcasts to help others find us!

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Development presents… May webinar

Posted by , on 9 June 2023

On 31 May 2023, Development hosted a webinar on the topic of in vitro and stem cell-based models of development. Below are the talks and Q&As hosted by our Executive Editor, Katherine Brown.

Anchel de Jaime Soguero (COS, University of Heidelberg)

Talk and Q&A by Anchel de Jaime Soguero


Elena Camacho Aguilar (Rice University)

Talk and Q&A by Elena Camacho Aguilar

Tyler Huycke (UCSF)

Talk and Q&A by Tyler Huycke
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Results from the Node postcard competition

Posted by , on 8 June 2023

One of the crowd-favourite giveaways here at the Node is our collection of postcards. With our supplies dwindling, we are planning to reprint some of the postcards, and take this opportunity to add some more #devbio favourites to our collection.

We ran a public vote for the Node postcard competition to select the top four images to be printed on new postcards. The image with the most votes will also be featured on the cover of a ‘Development’ issue in 2023.

We are now delighted to announce the results:

Winner: Xenopus laevis skeleton

Skeletal staining (alizarin red and alcian blue) of a Xenopus laevis at stage 62. Stage 51 larva was treated with a Cyp26a inhibitor during forelimb regeneration. Notice proximo-distal duplication in the left forelimb. Credit: JC Marin-Llera and MV Chimal-Montes de Oca

The winning image and the following images will be printed on new postcards:

Catshark embryo
Ventral view maximum intensity projection from an immunofluorescence staining labeling the developing nervous system (primarily nerves and ganglia) of a stage 30 small-spotted catshark embryo (Scyliorhinus canicula). The image was acquired using a ZEISS LSM980 with Airyscan2 confocal microscope, stitched and processed using ZEN software from the same microscope. Credit: E. Escamilla-Vega
Drosophila larvae
This shows heat-fixed Drosophila larvae expressing an infrared fluorescent protein (IFP) in the tracheal system using the Gal4/UAS system. Images were acquired in a confocal microscope (Nikon A1R+) with a 10x objective and using the mosaic modality. Stitching was done using the microscope’s software (Nikon NIS-Elements). The images were Z-projected and pseudocolored in Fiji and further processed using Inkscape. Credit: D. Rios
Arabidopsis leaf
Cells on the epidermis of a 3 day old Arabidopsis leaf. This is an adaptation of a linocut print created based on a microscopy image. Credit: M. Smit

Congratulations to the winners of the competition.

Thank you to everyone who submitted their images to the competition, and to everyone who participated in the voting.

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