With its growing adoption in the laboratory, an electronic lab notebook, or ELN, can be a useful tool to aid research, whether in academia or industry. But I found there is limited information on the practicalities of an ELN in a wet lab. Wet labs are a messy business, so strict guidelines are put in place to prevent spillages and contamination. It may seem absurd to use digital software while conducting experiments with innumerous hazards, but do the benefits of using an ELN in a wet lab outweigh the risks?
The majority of wet labs use paper lab books, few of which transfer the information from paper to an ELN. This is largely due to hazards involved in a wet lab, where the use of laptops or desktops is ill-advised. It is much easier to simply use a paper lab book, to keep all live results jotted down on paper. Furthermore, if a wet lab is using an ELN, scientists still have to use a paper lab book and copy some research notes into their ELN software at a later date.
A large issue with using an ELN in the wet lab is the inaccessibility of protocols. It is effortless to bring a paper lab book into the lab to see past results or double check some protocol results. But there is much more difficulty when it comes to ELNs since you can’t bring your laptop into the lab.
Nevertheless, the inability to bring a laptop into a wet lab isn’t the be all and end all. Some wet labs use tablets that are shared by several scientists, allowing protocols and previous research notes to be easily accessed while working.
The need for stringently organized lab books in wet labs can make ELNs seem more appealing. Many labs have strict controls on the data’s structure, to ensure its integrity and compliance. Some ELNs offer a dedicated templates section in which scientists can store and reuse their laboratory protocols and/or SOPs. Moreover, since those protocols are usually shared amongst several scientists, an ELN can help to guarantee that each colleague has access and is using the correct protocol. This maintains a consistent project structure, for easier research organization.
Moreover, by having a system in which team members can collaborate in real time, I saw supervisors providing immediate feedback through the use of an ELN. No more meetings had to be scheduled for supervisors to check content from paper lab books. That is the beauty of storing laboratory data online in an electronic lab book. Projects can be accessed from anywhere in the world. This also means that when a group member leaves a project, the data will not be lost with the person carrying the lab book.
As the lab is becoming more digitized, ELNs harness the simplicity of data transference. I could simply drag and drop digital data from microscopy images straight into an ELN. Hence removing the need to print and stick these results into a paper lab book. Moreover, with an ELN, data is more organized and easily accessible through advanced search and filter options.
ELN technology has recently advanced to the point where voice recognition can be converted into text, and there are countless other advancements in ELNs that are making the wet lab much more productive and efficient. For example, as the integration of laboratory data directly into ELNs becomes more likely to occur, the benefits of ELNs will be hard to miss up on in the wet lab. If you work in a wet lab and are searching for a solution, this comprehensive guide of the best electronic lab notebooks may prove useful.
During development, mechanical forces sculpt tissues into myriad forms. Actomyosin contractility generated within the cell has an increasingly appreciated role in this process, but how tissue forces relate to the physical properties of the extracellular matrix is still poorly understood, particularly at longer time scales and the whole tissue level. A new paper in Development addresses these issues using Drosophila leg development as a model, taking advantage of an ex vivo culturing method. We caught up with first author Amsha Proag and last author Magali Suzanne, group leader at the Centre for Integrative Biology in Toulouse, France, to hear more about the story.
Amsha and Magali
Magali, can you give us your scientific biography and the questions your lab is trying to answer?
MS My main motivation is to push the limits of our knowledge, probably like many scientists. I discovered Drosophila during my PhD and was amazed by the power of this model organism. I then focused on how tissues acquire their shape or morphogenesis, an aspect that still fascinates me. I grew up surrounded by artists, and particularly sculptors, and this probably had an influence on the fascination I have for tissue shapes. When I settled my lab in 2011, we were initially working on a particular aspect of morphogenesis, the impact of cell death on the final shape of a tissue. We discovered that dying cells mechanically influence their surroundings and this led us to the biomechanics field. We are now interested more broadly in how mechanical signals are integrated at the tissue level to generate new shapes.
And Amsha, how did you come to work in the Suzanne lab, and what drives your research?
AP What first attracted me was hearing Dr Suzanne explaining some of her findings at a conference, which demonstrated how tissue shape arose from exquisite mechanical communication between cells. I remember asking her after the talk about the mechanisms behind this spatial patterning, which strongly appealed to my background in soft matter physics. The interplay between physical forces and adaptive biological behaviour remains a fascinating topic for me and a major question in developmental biology.
The interplay between physical forces and adaptive biological behaviour remains a fascinating topic
Why are cultured Drosophila leg discs a useful system to understand developmental mechanics? Was much known about the forces driving eversion before your work?
AP & MS First, the leg disc constitutes an isolated tissue that is able to pursue its development ex vivo, and is thus directly accessible to live microscopy and micro-manipulation. Second, it is small enough for multiscale investigation of morphogenesis (integrating cytoskeleton dynamics, single cell behaviour and tissue-scale mechanics).
Although little was known before our work on leg disc eversion, a few papers described the process of wing disc eversion, showing on one hand that Myosin II was important for peripodial epithelium (PE) opening and eversion, and on the other hand that extracellular matrix (ECM) was being degraded in order to allow disc eversion. The novelty in our paper, following the dynamics of both the ECM and the cell monolayer of the PE, is the discovery of their physical and mechanical uncoupling during leg disc eversion.
Can you give us the key results of the paper in a paragraph?
AP & MS The leg disc is enclosed in the PE, a thin tissue that opens, contracts and is removed to allow the leg to evert. Our work shows that the tension produced by the growing leg disc on the PE is at first mainly borne by the ECM. But as the leg elongates, the ECM and cell layer are progressively uncoupled and tension builds up in the cell monolayer. Then, each layer of the peripodial epithelium withdraws by a different mechanism. The ECM layer is opened by local proteolysis and its tension completes its removal, whereas the cell monolayer opens and is removed by Myosin-II-dependent contraction, independently of ECM degradation.
How do you think the PE cells stay alive and seemingly happy without attachment to the ECM?
AP & MS This came as a surprise at first: seeing holes form in the ECM layer while the PE cells remained cohesive told us here was something worth investigating. Indeed, single adherent cells will tend to undergo apoptosis when cultured on non-adhesive or soft substrates. However, PE cells remain attached to each other after the detachment from the ECM. In addition, apoptotic markers only appear at the free edge of the PE, where cells have lost half their intercellular adhesion as well as their contractility. Hence, we hypothesise that intercellular adhesion compensates for basal adhesion by providing sufficient survival signals, through a combination of adherens junction signalling and mechanotransduction. This in vivo behaviour recalls recent observations on cultured cell suspended monolayers and calls for characterisation as a general property of epithelia.
Cell-cell junctions in the peripodial epithelium were visualised with fluorescent α-catenin and colour-coded according to their orientation.
When doing the research, did you have any particular result or eureka moment that has stuck with you?
AP The first time I saw individual collagen structures, fibre bundles, changed my perception of the ECM basement membrane. I grasped how thin it was compared to my previous view of ECM as an amorphous gel. It made me consider other properties of the layer, such as the propensity to form holes, but also the ability to resist tension. It was also a nice demonstration of the benefits of looking at the same system using different setups.
And what about the flipside: any moments of frustration or despair?
AP Investigating a living tissue requires some patience as several technical requirements have to be met simultaneously and even successful experiments tend to bring out the complexity of the tissue. Thankfully, these challenges also act as spurs.
So what next for you after this paper?
AP I plan on developing quantitative approaches for the life sciences, mainly through automated image and data analysis. In addition, I wish to contribute to bridging biology and physics and am currently working on undergraduate teaching material to this aim.
Where will this work take the Suzanne lab?
MS Until now, we were focusing on single cell dynamics and their influence on their direct surroundings, at a local scale. The study of peripodial epithelium dynamics opens new doors in considering biomechanics at the tissue scale.
Finally, let’s move outside the lab – what do you like to do in your spare time in Toulouse?
AP A little badminton, a lot of reading. I am also involved in the Human Library organisation, which aims at removing barriers between people through conversation.
MS Toulouse is a very nice place for hiking with the Pyrenees close by. There is also a number of festivals I enjoy a lot such as the Short Film festival, the image festival ‘MAP’, or the jazz festival ‘Jazz sur son 31’.
Of all the charts being ridiculed at WTFviz, many get shamed for their lack of a zero-baseline. When teaching DataViz, zero-baselines are invariably a topic of debate. The rules about zero baselines are necessary are often unclear. Therefore, let’s quickly recap.
Bar charts: always show zero
When we ecnode amounts by length, as done in bar charts, the zero-baseline is critical to reading the data. A bar twice as long represents that the category has twice the amount of counts. The number of the prestigious ERC starting grants to German host institutes roughly doubled from 2013 to 2014, correctly encoded by a bar twice the size in 2014 (A).
When the y-axis does not start at zero (B), the increase from 2013 to 2014 is over-emphasized and looks 4 to 5 times bigger. In (C) the baselines even starts above the first data point. This leaves entire bars out of the graph. The result is, that it appears as if only Germany received ERC funding in 2018.
Non-zero baselines (and also axis-breaks in bar charts!) skew the relative difference between categories and mislead. But non-zero baselines are often used to save space, not to intentionally mislead. Then, the chart could simply be shown with a less overall height. This option maintains the relative bar sizes faithfully. When reading bar charts we are always interested in relative, not absolute size differences among our categories. (And I learned that Israel is part of the ERC funding consortium!)
Number of submitted ERC grants varies a lot across countries. Varying the physical height of the plot faithfully maintains the relative differences.
Line charts are happy without zero
The situation is entirely different for line charts. We use them to show trends, e.g. increase or decrease in categories over time. The rate of change is encoded by the line slope relative to the horizon. For this, its distance to zero is not critical. Even without the zero-baseline we see that ERC success in Germany fluctuates, while UK and France have stable funding rates. And, no matter where the zero-baseline is, why does the UK have such a curious funding peak in 2012, what happened there!?
For understanding trends in line charts, we do not critically need to see the zero baseline.
Sometimes showing zero is misleading
Showing a zero-baseline is sometimes misleading in line charts. Think of a fever curve with a human body temperature scale from 0-100 ˚C. Such a scale would prevent us from seeing a life-threatening increase of 1˚, from 39 to 40˚C in a patient. Similarly, showing global temperatures at a scale from 0 to 120˚C results in an entirely flat line. It was used by opponents of climate research to hide man-made global temperature changes. (And an outcry at twitter swiftly followed).
Line chart misleading BECAUSE of a zero-baseline. Tweet: @EcoSenseNow, 23rd April 2019
Distributions: it depends on the data
When showing statistical summaries, again the zero often is not necessary to be visible. We are interested in the shape of the data (normal or bimodal), it’s median, and outliers. How far the majority of data points are from zero is not usually of interest as long as all data is shown. Instead, the relative distance of individual data points from each other are key.
Good practice for non-zero baselines
When using non-zero baselines, the common practice is to unlink the x- and y-axes. For educational purposes I cut data from the right example. This is a dangerous territory and in some cases may be misleading the audience. In this example, I effectively hide the early lead of the UK in winning ERCs!
One possibility to alert readers to a non-zero baseline in your charts.
What did Watson and Crick discover? Rosalind Franklin’s notes…
In this episode from our centenary series exploring 100 ideas in genetics, we’re unravelling the story of the double helix, cracking the triplet code, and sketching out a Punnett square.
If you enjoy the show, please do rate and review and spread the word. And you can always send feedback and suggestions for future episodes and guests to podcast@geneticsunzipped.com
We are looking for a lab technician to assist in research on muscle stem cells, development, regeneration, disease, and evolution. More details about our research can be found at http://www.kardonlab.org/. Technician will assist in management of a mouse colony and conduct supervised research (leading to publications). Technician must be reliable, well organized, detail-oriented, excited about research and committed to working in our lab for at least two years. Prior lab experience is preferred (although not necessarily required), and class work in biology and enthusiasm for science is essential. Lab is located at the University of Utah in Salt Lake City, affording amazing opportunities for science and outdoor recreation. Looking for someone to start July-August 2019.
Contact Gabrielle Kardon (gkardon@genetics.utah.edu) with CV, list of references, and a brief statement about why you are interested in the position. BS or BA required.
One of the most obvious examples of left-right asymmetry in animal bodies comes from snails: in most species or strains, the shells coil dextrally, but some coil sinistrally. The control of coiling is genetic and begins in the early embryo. Previous work has implicated the formin diaphanous in the regulation of snail shell chirality, and a new paper in Development now decisively proves its involvement, thanks to the first application of CRISPR/Cas9 gene knockouts in molluscs. We caught up with the author team behind the paper: Masanori Abe and his supervisor Reiko Kuroda, Professor at Chubu University in Japan (recently moved from Tokyo University of Science), to find out more.
Masanori and Reiko
Reiko, can you give us your scientific biography and the questions your lab is trying to answer?
RK During my PhD at the University of Tokyo and in my first postdoc period under Professor Stephen Mason at King’s College London, I studied chemistry and carried out research on X-ray crystallography and spectroscopy of chiral transition-metal complexes. I then moved to the Biophysics Department and worked on DNA-carcinogen/anti-cancer drug interactions using X-ray crystallography, computer graphics and molecular biology. I was appointed Associate Professor and then Professor in the Department of Chemistry, Graduate School of Arts and Sciences of the University of Tokyo, and then recently, Professor at Tokyo University of Science, and very recently Professor at Chubu University. As I am interested in chirality, my attention was inevitably drawn to the molecular mechanisms of snail coiling. I was a late starter in developmental biology, but I have now been working in the field for the last 20 years.
In 1999, I coined the term ‘chiromorphology’, which expresses the concept of my research to link macroscopic and microscopic morphological phenomena in both biology and chemistry domains through chirality. Eventually, I hope to understand the impact and the origin of the homochiral biological world [in which almost all proteins contain only L-amino acids and RNA/DNA contain D-(deoxy)ribose]. In the area of crystallography, I try to understand how billions of molecules gather to form either chiral or non-chiral crystals, and how chirality is generated, recognized, transferred and/or amplified in the solid state. In the area of spectroscopy, I have developed chiroptical spectrophotometers, which can analyze samples in their condensed phases, such as the crystalline, gel or membrane state, without the contamination of artefact signals that can arise from intrinsic macroscopic anisotropy. We have used them to explore the dynamics of secondary-structure transitions of β-amyloids and hornet silk, in addition to the crystals of organic and inorganic compounds.
Masanori: how did you come to work with Reiko, and what drives your research?
MA When I joined the Kuroda lab at the Graduate School of the University of Tokyo, I was initially not interested in snails at all, but in research on DNA recognition compounds and proteins. However, when this research got stuck, I decided to participate in the snail work. And so far, I have been very excited about the elucidation of the mechanism of left-right asymmetry determination in snails. I think it suits me to look for interesting things that people don’t normally pay attention to, and this research is just that.
How was Lsdia1 first implicated as a candidate chirality gene, and why did you need to turn to CRISPR/Cas9 to prove its role decisively?
RK We reached the conclusion that Lsdia1 is the strongest candidate for the handedness-determining gene based on positional cloning and on various experiments using pure dextral, pure sinistral and F10backcrossed lines that we had established. We found a sinistral strain that carries a frameshift mutation that abrogates full-length LsDia1 protein expression, already at the one-cell stage (published in 2016). Although we were confident about the results, we could not ignore the possibility that Lsdia1 is simply genetically linked to a true handedness-determining gene. We envisaged that directly knocking out the Lsdia1 gene by CRISPR/Cas9 would provide proof. Although only MA and RK are involved in the current work, we are grateful to all the scientists/technicians/students who worked in Kuroda lab in the past for their contributions in establishing pure and backcrossed strains, rearing the snails, and enabling positional cloning, and to the Japan Science and Technology Agency (JST) for the funding of ERATO and SORST Kuroda Chiromorphology projects (to RK, 1999-2004 and 2004-2009, respectively).
Can you give us the key results of the paper in a paragraph?
RK We have decisively identified that Lsdia1 is the long-sought handedness-determining gene of Lymnaea stagnalis by developing CRISPR/Cas9 gene editing techniques. Biallelic frameshift mutations introduced into the gene produced sinistrally coiled offspring generation after generation, in the otherwise totally dextral genetic background. We could also show that the gene sets the chirality already at the one-cell stage, the earliest observed symmetry-breaking event linked directly to body-handedness in the animal kingdom. The early intracellular chirality is superseded by the intercellular chirality during the third cleavage, leading to asymmetric expression of nodal and Pitx (two genes known to regulate chirality in vertebrates) and then to organismal body handedness.
Gene-edited F1 snails (49-cell stage), with in situ hybridisation for nodal, DAPI staining and selected cell types labelled.
How do you think chirality is transmitted through development?
RK This is the project we are currently working on. It is interesting that intracellular chirality within a cell is superseded by the intercellular chirality during the third cleavage (as proven by the fate of mechanically manipulated embryos at this stage), and then to the 24-cell stage when the cell fates are determined. We hope to report the results in the near future.
Left-right (LR) asymmetry is a widespread feature of animals – how much conservation do you think there is at a genetic and cytoskeletal level?
RK Although diverse mechanisms have been proposed for different phyla, we think a unified mechanism and the involvement of cellular chirality are probable. We believe that there are conserved mechanisms, given that diaphanous is a protein present in many phyla. We plan to elucidate the mechanisms of chirality establishment by LsDia1 at the molecular level, which may give insight into the conservation of the genetic control of LR asymmetry.
Do you have any advice for people wanting to do CRISPR/Cas9 mutagenesis in snails?
RK Gene editing of snails by CRISPR/Cas9 is not particularly difficult, as the method itself has already been well established in model organisms, although one must check for possible off-target effects. The most difficult aspects are raising the gene-edited eggs to adult snails, and successfully breeding them to produce the next generation.
When doing the research, did you have any particular result or eureka moment that has stuck with you?
MA Since it was not guaranteed that Lsdia1 was a handedness-determining gene, I sometimes felt anxious that the gene-edited individuals created over a long period would be wasted. At that time, the F0 individuals were starting to lay eggs, so I decided to count the number of hatched juveniles, and then felt a strong sense of discomfort. At that moment, I realized that the direction of shell coiling had changed into sinistral! The juvenile snails did not show any abnormalities except the shell-coiling inversion and moved around in the Petri dish vigorously. Then I was finally convinced that Lsdia1 was the snail handedness-determining gene. I was also surprised when I found one-cell-stage chirality.
And what about the flipside: any moments of frustration or despair?
MA It can sometimes get frustrating spending a long time caring for snails. Containers for breeding individuals should be cleaned weekly, thus I can’t take a long vacation leaving the lab. In particular, we are doing experiments with eggs just laid, but the quality of the eggs varies greatly depending on the health and age of the parent snail. It can’t be avoided in order to obtain correct results with good reproducibility.
So what next for you after this paper?
MA It was found that LsDia1 and LsDia2 give LR asymmetry to the one-cell-stage egg. Next, I would like to investigate the origin of organismal LR asymmetry by delving into the molecular level, focusing on the analysis of cytoskeleton dynamics in snails. In addition, as we have established gene editing technique for this snail, which is often used in the study of perception, learning and memory, I hope that our work will help this snail become a model organism for human disease research.
Where will this work take the Kuroda lab?
RK When one question is solved, more questions arise. We have so many interesting themes to work on: the roles of LsDia1, LsDia2 and other proteins in explaining the Dia1-dependent chirality within one cell, how the intracellular chirality is superseded by intercellular chirality (cytoskeletal dynamics), and how information on the geometrical arrangement of blastomeres at the eight-cell stage is transferred to the 24-cell stage, and then to nodal/Pitx expression, at the molecular level. My long-term research objective is to link macroscopic and microscopic phenomena, i.e. across the biological hierarchy, using chirality.
When one question is solved, more questions arise
Finally, let’s move outside the lab – what do you like to do in your spare time in Tokyo?
MA I like taking a walk doing Pokemon Go as it is good exercise. There are many interesting spots in Tokyo, historic buildings and monuments, beautiful parks and cityscapes, etc. Also I enjoy making small discoveries and surprises even in the back alleys that I had never visited before.
RK We both moved this April from Tokyo to Chubu University, Kasugai, near Nagoya. When in Tokyo, I often went to special exhibitions at the National Science Museum (such as ‘Lascaux – Cave Paintings of the Ice Age’, ‘Hunters of the Ocean’, ‘Wine – the Exhibition’ and ‘Deep Sea’) or other museums and galleries in Tokyo (e.g. ‘Winne-the Pooh – the original drawings from the V&A Museum London’ and ‘Vermeer and Dutch Art’). Once we have settled down in Kasugai, I hope to explore the area.
We are seeking a proactive and highly motivated individual to deliver gene targeting capabilities at the Babraham Institute.
The successful applicant will work closely with scientific staff and will be embedded in the Epigenetics Programme in order to foster a strong scientific working environment, whilst providing expert support in gene targeting and delivering transgenic mice to researchers across the Institute. Opportunities for training and to participate in significant high impact research leading to publications will be available.
The ideal candidate will have enthusiasm and proven experience with developing and applying molecular biology technologies in genome engineering. This includes being able to independently design, generate and use gene targeting reagents and recently developed editing technologies such as CRISPR/Cas9. The ideal candidate will possess the skills required to independently undertake all aspects of the generation of transgenic mouse embryos. Prior experience with embryo collection and electroporation is highly desirable.
The job holder will be expected to compile, maintain, interpret and present data. Strong communication and interpersonal skills will be essential in order to clearly present technical and conceptual information verbally and in writing to a wide variety of people with varying levels of knowledge.
Closing date for applications is Thursday, 4th July 2019.
NO AGENCIES PLEASE
The Babraham Institute holds a silver Athena SWAN award and is committed to promoting and developing a culture of excellence, diversity and mutual respect that supports the Institute’s ambitions and attracts highly motivated and talented people. The Babraham Institute is a Disability Confident Employer and has a positive approach to employing disabled people.
Rita Levi-Montalcini Postdoctoral Fellowship in Regenerative Medicine
The Center of Regenerative Medicine at Washington University in St. Louis invites applications for the Rita Levi-Montalcini Postdoctoral Fellows in Regenerative Medicine program. These fellowships honor Rita Levi-Montalcini, whose Nobel-winning discovery of Nerve Growth Factor was among the first regenerative biology research to be done at WUSTL.
The Center of Regenerative Medicine (CRM) seeks individuals of outstanding talent with a doctoral degree to provide them with the opportunity to pursue research within a CRM lab. As Rita Levi-Montalcini was herself an international scholar working at WUSTL, we strongly encourage international applicants to apply. Additional information about the Center of Regenerative Medicine can be found at: https://regenerativemedicine.wustl.edu/. Full program details can be found at: https://regenerativemedicine.wustl.edu/about/rlm_fellowship/rlm_programdetails/
Qualifications
RLM Fellowships are intended for exceptional scientists of great promise who have recently been awarded, or who are about to be awarded, the doctoral degree. Fellows are required to work in the lab of a CRM faculty member on a project that directly focuses on regenerative medicine. Current employees, fellows, and students of Washington University in St. Louis are not eligible. Applicants currently on H-1B visas are not eligible. We anticipate awarding two fellowships in 2019.
Terms of Appointment
RLM Fellowships will be granted for a period of two years.
A Ph.D./D.Sc./M.D. must be awarded and proof furnished to the CRM before the start of the Fellowship.
The RLM Fellowship provides annual compensation of $55,000, as well as fringes and health insurance, research funds, and relocation and travel funds.
Review of applications will begin on August 30, 2019.
Please provide a full current CV, two letters of reference, indicate a potential CRM faculty host (https://regenerativemedicine.wustl.edu/people-page/), and a brief description of scientific accomplishments and long-term goals.
Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, origin, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status.
The Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute is an international centre of excellence for stem cell research and regenerative medicine. Scientists in the Institute collaborate to advance our knowledge of various stem cell types and to perform pioneering work in translational research areas, providing the foundation for new medical treatments (https://www.stemcells.cam.ac.uk/).
Research position is open in the laboratory of Professor Austin Smith to join investigations into the fundamental biology of pluripotency and pluripotent stem cells in different mammals. Candidates should have a publication record from their previous research, but prior experience with pluripotent stem cells is not essential. Homepage: www.stemcells.cam.ac.uk/research/pis/smith
We are looking for a Research Assistant/Associate – Bioinformatics. Research Associate candidates should have a PhD or equivalent research doctorate in computational biology or bioinformatics. Research Assistant candidates should have a Bachelor’s degree or higher in a relevant subject. The successful candidate will have experience in next generation sequencing pipelines, and good understanding of molecular cell biology. Under the guidance of a senior bioinformatician, you will develop and implement algorithms, analysis methods and visualisation tools for cross-species comparisons using scRNAseq, ChIPseq and ATAC-seq data.
Candidates will have proven capacity to design, execute, and interpret experiments or data analyses. Good communication skills and the ability to work effectively in a team are essential. Our lab is international in character and this project is collaborative with groups in China and Japan.
Fixed-term: The funds for this post are available for 2 years in the first instance.
Click the ‘Apply’ button below to register an account with our recruitment system (if you have not already) and apply online at http://www.jobs.cam.ac.uk/job/21931/
The closing date is 10 July 2019, with interviews to be confirmed.
Please ensure that you upload a covering letter and CV in the Upload section of the online application. If you upload any additional documents which have not been requested, we will not be able to consider these as part of your application.
Please include details of your referees, including email address and phone number, one of which must be your most recent line manager.
Please quote reference PS19502 on your application and in any correspondence about this vacancy.
The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.
The University has a responsibility to ensure that all employees are eligible to live and work in the UK.
The Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute is an international centre of excellence for stem cell research and regenerative medicine. Scientists in the Institute collaborate to advance our knowledge of various stem cell types and to perform pioneering work in translational research areas, providing the foundation for new medical treatments (https://www.stemcells.cam.ac.uk/).
Research positions are open in the laboratory of Professor Austin Smith to join investigations into the fundamental biology of pluripotency and pluripotent stem cells in different mammals. Candidates should have a publication record from their previous research, but prior experience with pluripotent stem cells is not essential. Homepage: www.stemcells.cam.ac.uk/research/pis/smith
Two positions are available, each for 2 years in the first instance:
Post 1: Molecular embryologist with specialist skills in culture and manipulation of pre-implantation embryos and post-doctoral experience studying blastocyst development and lineage segregation.
Post 2: Molecular biologist/biochemist with PhD training in the molecular genetic analysis of signalling pathways and downstream transcriptional regulation.
Candidates will have proven capacity to design, execute, and interpret experiments or data analyses. Good communication skills and the ability to work effectively in a team are essential.
Our lab is international in character and this project is collaborative with groups in China and Japan.
Fixed-term: The funds for this post are available for 2 years in the first instance.
Click the ‘Apply’ button below to register an account with our recruitment system (if you have not already) and apply online at http://www.jobs.cam.ac.uk/job/20694/
The closing date is 10 July 2019, with interviews to be confirmed.
Please ensure that you upload a covering letter and CV in the Upload section of the online application. If you upload any additional documents which have not been requested, we will not be able to consider these as part of your application.
Please include details of your referees, including email address and phone number, one of which must be your most recent line manager.
Please quote reference PS18395 on your application and in any correspondence about this vacancy.
The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.
The University has a responsibility to ensure that all employees are eligible to live and work in the UK.
(8 votes)
Loading...
(No Ratings Yet)
(5 votes)
What did Watson and Crick discover? Rosalind Franklin’s notes…
