Rita-Levi Montalcini (1909-2012) was an Italian neurobiologist who lived an extraordinary life, and today (22nd April) would have been her 115th birthday. Click on each image to enlarge and read more about her…
Sources: Coutinho, L. and Teive, H.A.G. (2023) ‘Rita Levi-Montalcini: the neurologist who challenged fascism’, Arquivos de Neuro-Psiquiatria, 81(1), pp. 95–98. Available at: https://doi.org/10.1055/s-0043-1761426.
Hamburger, V. and Levi-Montalcini, R. (1949) ‘Proliferation, differentiation and degeneration in the spinal ganglia of the chick embryo under normal and experimental conditions’, Journal of Experimental Zoology, 111(3), pp. 457–501. Available at: https://doi.org/10.1002/jez.1401110308.
Malerba, F. (2022) ‘Why Are We Scientists? Drawing Inspiration From Rita Levi-Montalcini’, Frontiers in Cellular Neuroscience, 15, p. 741984. Available at: https://doi.org/10.3389/fncel.2021.741984.
Here in the Koltowska lab, we are interested in all things lymphatic vessel-related. How lymphatic endothelial cells (LECs) are specified, gain their identity, end up in the right place to form vessels, and how these vessels function.
Lab roll call
Hannah Arnold has been a postdoc in the lab for five years and is interested in lymphatic development, focusing on how LECs migrate and interact to navigate their environment.
Marleen Gloger has been a postdoc in the lab for five years as well and is interested in lymphatic vessel development, specifically LEC cell proliferation, and how these processes are altered in disease conditions such as cancer and metastasis formation.
Di Peng has done a PhD in the group and now continues as a postdoc. She is very fond of observing cellular events during development using different live imaging techniques. Her projects focus on regulation of lymphatic endothelial behaviours.
Faidra Voukelatou has recently started her PhD in the group and is interested in cancer as well as lymphatic vessel research. She enjoys working with zebrafish as an animal model to investigate the dynamics of brain cancer invasion and vasculature.
Renae Skoczylas has been a research engineer in the lab for 6 years and enjoys all things zebrafish and lymphatics. She is particularly happy generating new mutant lines for the lab using CRIPSR technology and being involved in and helping with any other lab members’ projects.
Favourite technique, and why?
KaskaKoltowska: Microscopy! There is something incredibly magical in looking down the microscope and observing life in high magnification. Using microscopy to look at zebrafish heartbeat and blood flowing through the vessels never stops to amaze me!
Apart from your own research, what are you most excited about in developmental and stem cell biology?
KaskaKoltowska: I think how gene expression is regulated and the steps coordinating cell specification is incredibly fascinating. The level of developmental reproducibility in every embryo is just mind-blowing. Biology gets it right almost every time, and if it does not, we can learn something very important.
How do you approach managing your group and all the different tasks required in your job?
Kaska Koltowska: I don’t think I use any specific managing tools. I dedicate time to discussing science with every member of the group regularly. This helps to keep the projects focused. When a project is coming up close to completion I dedicate more time for it. It helps a lot that the team is very efficient and group members can manage themselves very well so my input is minimal. For myself, I often make a weekly prioritisation plan of the most important tasks that need to be done that week and try to stick to it.
What is the best thing about where you work?
We are positioned between two wider communities. That of Vascular Biology, where our lab is located and encompasses ten research groups, and the Uppsala Zebrafish community where our fish are housed alongside five other groups and one service platform.
What’s there to do outside of the lab?
Uppsala is a small but busy student city where you can enjoy restaurants and cafes for a ‘fika’ break. It is located close to nature giving us the opportunity to enjoy the forest for a walk or BBQ in the summer and snow sports in the winter. It also provides an excellent backdrop for walking the boss’ dog. On the other hand, Uppsala is a short train ride to Stockholm so it is easy to enjoy big city life on the weekends and go to museums, theatres or concerts.
Science communication is an integral part of being a researcher. Want to practice your science writing and presentation skills? Register to attend “SciCommConnect: Science communication, community connections“!
The three community sites supported by The Company of Biologists – the Node, preLights and FocalPlane – are hosting a free, online event on Monday 10 June 2024 from 13:00-18:00 BST, focusing on the different ways in which science can be communicated. We hope this event will present a unique opportunity for you to work on your science writing and presentation skills and connect with your peers across the world in a friendly, informal environment.
Dr Jamie Gallagher is an award-winning science communicator, trainer and consultant. He will share tips and tricks on how to make science talks as interesting, engaging and memorable as possible.
Three minute research talk competition
Similar to the Three minute thesis competition format, this is a chance for you to practice communicating your research in a concise and engaging way.
Present your work for a chance to win a cash prize and to get feedback from Jamie, who is a previous Three Minute Thesis winner. To enter please provide us with short summary of your intended talk (think about how you would advertise your talk in a tweet!)
Applications for the short talk competition are optional and spaces are limited. Deadline for entering into the competition is Sunday 26 May.
Those who do not wish to give a talk will also benefit from listening to people’s talks and Jamie’s feedback. They will also be able to vote for their favourite talk.
#DevBiolWriteClub and themed writing sprints
Prof John B. Wallingford is a Professor at UT Austin. He is passionate about writing and has written on the Node regularly, including the popular #DevBiolWriteClub posts. He will share some excellent writing advice which can directly be applied during the themed writing sprints that will follow.
For the writing sprints, you can pick which group to be in (the Node, preLights or FocalPlane). Each group will work together to brainstorm and draft a piece of writing on a pre-selected topic. Details of the writing briefs for each group will be provided closer to the event.
It’s challenging to sustain a pregnancy when food is short, or conditions are otherwise tough. That’s why many mammalian embryos can postpone their growth to get through periods of environmental stress and then re-enter development when conditions improve. This stalling of development is known as embryonic diapause, and understanding the mechanisms behind it might help improve infertility treatments, such as embryo freezing. Now, researchers at the Center for Excellence in Brain Science and Intelligence Technology, the Chinese Academy of Sciences in Shanghai, China, have discovered how nutrient depletion is sensed by embryos growing in hungry mouse mums to induce diapause. They publish their study in the journal Development on 11 April 2024.
A mouse embryo (blastocyst) that has paused its development due to nutrient depletion. Image credit: Jiajia Ye.
Lack of food is a known trigger of embryonic diapause, but it has not been clear how nutrient depletion in the mother’s diet is sensed by the embryo. “Seasonal starvation is one of the universal environmental stresses in nature,” explained Professor Qiang Sun, who led the study. “However, the regulatory process of diapause in early-stage embryos is not fully understood. So, we decided to examine whether nutrient deprivation induces embryonic diapause.”
By comparing hungry and well-fed pregnant mice, the team discovered that embryos in the hungry mice did not implant into the uterus and their growth paused at an early timepoint, when the embryo comprises a hollow ball of cells called the blastocyst. These embryos remained viable and could start developing again when transplanted into a well-fed mother.
To work out which nutrients were important to induce diapause, the researchers grew early-stage mouse embryos in dishes that contained different nutrients. They found that embryos grown in dishes lacking protein or carbohydrates paused their development, whereas the embryos exposed to normal nutrient levels did not stall and kept on developing. The scientists then went on to reveal that nutrient sensors in the embryo can detect drops in protein or carbohydrate levels, which triggers the entry into diapause.
The finding that embryos grown without protein or carbohydrates can pause their development means that they can survive longer in the lab. In the future, this finding might lead to improvements in fertility treatments, which currently include approaches such as embryo freezing. “We think our study can inspire the development of new methods for human embryo preservation,” said Professor Sun. “Embryo cryopreservation is a widely used approach, but there is still no consensus on when cryopreserved embryos can be thawed and transferred into the uterus. Many clinical studies have shown that traditional frozen embryo transfer can increase the risk of problems during pregnancy. Therefore, it is necessary to develop alternative methods to preserve embryos.”
Studies focusing on diapause may even have long-term implications for cancer treatments. “Dormant cancer cells which persist after chemotherapy resemble the diapaused embryos,” said Professor Sun. “Consequently, we hypothesize that delving into the mechanism of diapause may have positive implications for cancer treatment and decreasing the chances of relapse.”
Jiajia Ye, Yuting Xu, Qi Ren, Lu liu and Qiang Sun (2024). Nutrient deprivation induces mouse embryonic diapause mediated by Gator1 and Tsc2. Development, 151, dev202091 doi: 10.1242/dev.202091
This series of interviews features principal investigators (PIs) within the first five or so years of establishing their own research group. Through these conversations, Development aims to illustrate that there is not a ‘one-size-fits-all’ approach to securing an independent position and setting up a research programme. Discussing the challenges and difficulties new PIs have overcome and highlighting the best moments will hopefully offer encouragement to other ECRs and stimulate discussion around the career path of a developmental biologist.
Click on the pins to read the interviews:
Collage of all 30 interviewees in the ‘Transition in development’ series. (No Ratings Yet) Loading...
The lab is located in the southern part of the city of Lyon, France on the campus of the Ecole normale supérieure (ENS). It is one of the CNRS research groups at the Institute of Functional Genomics of Lyon (IGFL), which focuses on integrating developmental, physiological and evolutionary perspectives to study complex biological systems at the organism level.
Research summary
Our group studies the morphogenesis of brown algae. These organisms, some of which can grow to 45 m long, have evolved independently of plants and animals. We work on identifying the strategies that these organisms have adopted to establish their body plans at the embryogenesis stage. In particular, right now we are exploring which mechanisms control the position and the orientation of cell division planes. When, where and how do cells grow and divide is our current obsession!
This question is even more challenging when applied to our favourite models: brown algae are largely understudied multicellular organisms that possess unique cellular components – in terms of cell wall composition, vesicular and cytoskeleton organisation, pigments – resulting from their distinctive evolutionary pathway.
Lab roll call
Marie Zilliox: I’m a post-doc with a PhD in zebrafish development, and my mission is to monitor the growth of brown algal embryos in real time and in 3D using light-sheet and multi-photon microscopy techniques to determine whether cell shape controls the orientation of cell division. The challenge lies in mastering long-term time-lapse microscopy in 3D and in culture conditions compatible with the natural environment as well as with the presence of pigments that diffract light.
Tanguy Dufourt: I’m a first-year PhD student. I hold a Master’s degree in Systematic Evolution and Paleontology (MNHN Paris), and I am now studying which intracellular mechanisms are involved in the orientation of cell divisions in brown algal cells. I aim to model the displacement of centrosomes during the cell cycle by using software based on the forces generated by and applied to dynamic microtubules. First, I need to adapt this software to the cell biology of brown algae: cuboid cell shapes, presence of chloroplasts and vacuoles, throughout the cytoplasm, etc.
Alexis Martel: I have a Master’s in Marine Biology (Toulon University, France), more specifically in biotic interactions and anthropogenic disturbances in marine environments. I work here as a lab manager. In the past 6 months, I have set up most of the lab equipment and the cultures of the brown algal models of the team at IGFL. Now I am developing new microfluidics protocols for the forthcoming team projects involving this technique.
Bernard Billoud: I am a lecturer with a 30-year university career in bio-informatics, and a researcher working on modelling and simulation of algal development. My role is to develop mathematical and mechanical models that can account for the tissue patterning in brown algae. As a member of a team where most of the members are involved in experimental work on the bench, I can assess the time and technicality required to obtain the quantitative data on living organisms that I need to validate the models.
Bénédicte Charrier: I am the leader of the team. As a former land plant biologist, I have been studying the development of brown algae for the past 20 years. I come from a molecular and genetic background, and I am now studying the role of mechanical forces in the process of morphogenesis. Interdisciplinarity combining cell biology with mathematics and physics is what I need to answer my questions.
Favourite technique, and why?
Bénédicte: I love penetrating the microscopic world! Beyond observing live or fixed, labelled or even unlabelled cells, I am now captivated by microinjection. It provides me with an “intimate” moment spent with the algae. Microinjection requires lengthy preparation, but when the time has come for the needle to touch the cell surface, then the conversation starts. And in this specific context, different cells of brown algae reveal their lot of unexpected responses!
Apart from your own research, what are you most excited about in developmental and stem cell biology?
Bénédicte: I’d like to understand the complexity of biological systems. How do cells co-ordinate themselves or work together to form an organism? How do the different molecules that make up a cell interact to produce a functional living cell? What are their priorities and tolerances (plasticity, robustness)? What are their molecular and chemical bases? What role does self-organisation play? I like to think that the mechanisms of life are based on simple mechanistic rules, perhaps made up of complex interactions at the chemical/molecular level.
How do you approach managing your group and all the different tasks required in your job?
Bénédicte: I would like to say that I’ve taken part in training courses organised by my employer to help me manage all the tasks I have to do as well as possible, but that’s not the case! We’re a small team with a large proportion of young scientists in training. The belief that we’re all highly committed and motivated by our work is undoubtedly the cement to building trust among everyone on the team. My favourite time of the week is when I’m fed fresh results, straight from the oven!
What is the best thing about where you work?
Marie: The mutual support between the people from different research teams at the IGFL and also between the different laboratories and facilities located in Lyon (e.g. the Reproduction and Development of Plants laboratory, the PLATIM Imaging Core Facility) is a real help and relief especially when setting up a new laboratory, which is the case for the Charrier lab, which recently joined IGFL.
Tanguy: A good point of the lab is that the infrastructure is recent and the office is very close to the lab.
Alexis: Our laboratory is affiliated with the ENS, which is a renowned research centre with a vibrant and stimulating environment. The lab itself is recent and bright, so it’s quite pleasant to work in. The different teams work on very varied and interesting subjects.
Bernard: The lab is a very stimulating environment, where people work very seriously in a relaxed and enthusiastic atmosphere.
Bénédicte: The IGFL is an open-minded institute, with a wide variety of model organisms and scientific questions. You would think that this diversity would be unsettling or uncomfortable on a day-to-day basis when it comes to technical difficulties or model-specific issues, but it’s not. Open-mindedness is the DNA of the IGFL! What’s more, the institute is currently broadening its approach to developmental biology and recruiting new teams from different backgrounds. The fact that the IGFL is located on the ENS Lyon campus means that there are plenty of seminars by world-class scientists. The campus is quite cosy, but still offers a wide range of opportunities to develop my project in excellent conditions.
What’s there to do outside of the lab?
Marie: The laboratory is perfectly situated close to the Lyon city centre, where there are plenty of restaurants and bars to suit all tastes, from a cozy café to a night of dancing. Lyon is full of things to do, with an exhibition or concert every weekend. Lyon’s location is also ideal for getting to cities such as Annecy, Marseille, Paris and Geneva in less than 2 hours, which is perfect for weekend getaways.
Tanguy: In Lyon there are a lot of cultural places, like concert halls or museums.
Alexis: Lyon is a very pleasant city. The “Vieux-Lyon”, which is the old quarter in the city centre is a pleasant place to wander around. Also “le Parc de la Tête d’Or” is a peaceful green city park, where I like to go for walks.
Bernard: Lyon has all practical and cultural facilities of a big city, with relaxing natural sites nearby.
Bénédicte: The Alps being less than 2 hours’ drive away is a major advantage when you like to be outdoors, in the mountains. The Mediterranean Sea isn’t that far away either. Lyon is also actively developing its cycling infrastructure, with greenways available in the near future. This is a considerable advantage when, like me, stopping taking the car to work becomes a daily priority.
In this SciArt profile, we meet Maja Mielke, who is doing a PhD in functional morphology and enjoys making nature-inspired drawings.
Saharan horned viper (pen and ink). I used a photo by National Geographic photographer Joel Sartore as reference for this illustration.
Can you tell us about your background and what you work on now?
Currently, I’m pursuing a PhD in functional morphology at the University of Antwerp (Belgium), but originally, I’m from Germany. I did my bachelor’s in Molecular Biophysics in Berlin but switched to Biology & Evolution for my master’s because I wanted to study animals rather than molecules. This way, I discovered my fascination for the functional morphology and biomechanics of vertebrates. I studied squirrels for my master’s thesis and switched to birds for my PhD. I’m exploring how they move their beak while processing, cracking, and husking seeds.
Skull of a Java finch (digital illustration made in Krita). I rarely do digital illustrations, but the flexibility that comes with working digitally comes in handy for work-related illustrations. I made this drawing to pitch my PhD project to a research foundation. It worked! They are now funding my PhD.
Were you always going to be a scientist?
Pretty much, yes. Especially the natural sciences have always sparked my interest and fascination for the world around us. Becoming a scientist was the logical step after finishing school. The hardest part was to choose a field of study! But the interdisciplinary Biophysics bachelor program was the perfect fit at that time.
And what about art – have you always enjoyed it?
I have practiced and enjoyed art a lot during my school years. But once I started my bachelor studies, I was so occupied with lectures, practicals, and exams that there was hardly any room for creating art. Only during my masters did I attempt to re-integrate an art practice into my life. Unfortunately, while managing work, family, and other hobbies, I still haven’t managed to practice art on a regular basis until now. But whenever I finish a piece, it makes me really happy.
Great horned owl (pen and ink). This owl was one of the first more elaborate pieces I did with pen and ink. I used a photo by National Geographic photographer Joel Sartore as a reference for this illustration.
What or who are your most important artistic influences?
First, my art is primarily inspired by nature and my fascination for the animal world. That determines my ideas on what to illustrate in the first place. Second, I’m inspired by the work of both professional artists (like Ben Rothery, Alphonso Dunn, Denise Soden, and Raoul Deleo) and fellow hobby artists that share their work online. Studying their art influences my own artistic approaches and inspires me a lot. Third, my art is highly influenced by the work of wildlife photographers, whose beautiful work I use as references for my drawing practice. Last, I’ve been highly influenced by drawing and painting courses that I attended during my life, be it a painting class for children during primary school, more advanced naturalistic drawing classes during high school, or a scientific illustration course I attended two years ago during my PhD.
Skull of a Triceratops (pencil).Pencil is the medium I feel most comfortable with. I made this illustration after following the “Scientific Illustration” course by Oscar Sanisidro from Transmitting Science in 2022.
How do you make your art?
Most of the time, I draw from reference photos. I usually work in pencil or ink, sometimes with watercolor, and very rarely digitally. Because of my limited free time during normal working days, I often finish a drawing during multiple sessions spread over the evenings of several days. If I’m not working on an elaborate piece, I mostly just practice some basic drawing skills with quick sketches in my sketchbook.
Red fox (pen and ink and watercolor in sketchbook). This fox started as a quick sketch in my sketchbook, without the intention to ‘finalize’ it in any way. But sometimes, drawings make their own developments.
Does your art influence your science at all, or are they separate worlds?
Sometimes, it’s more the other way around: my science influences my art. I create my own scientific illustrations, e.g., for conference presentations or papers. Also, because I study birds, I particularly enjoy drawing them. But most of the time, my art is doing its own thing, just exploring the animal world and pausing for a sketch whenever inspiration hits.
Common house mosquito (pen and ink in sketchbook). Normally, I’m more interested in vertebrates (both for my research and as my drawing subjects). But insects are just so much fun to draw. The reference photo for this drawing is from Rick Delhem’s book “Een boek vol insecten”.
What are you thinking of working on next?
I would like to further improve my pen and ink drawing skills and explore drawing with dip pens. I love the minimalistic approach of using only black ink as a medium instead of dozens of colors. Visualizing different textures like feathers, fur, and scales with only ink lines or dots is challenging but also so much fun.
Do you study Development? Complex cellular systems? Cancer? Then we’d love to have you at our Meeting “Cell Lineages across scales, space and time”!
Lineage trajectories and cellular decisions coordinate embryonic development and growth. Similarly, cellular origin and clonal trajectories are crucial elements to understanding and modulating the response to injury, disease, and therapies. This meeting will showcase cutting-edge research spanning developmental biology, regeneration, and cancer, in light of recently developed techniques to study lineage trajectories and cellular decisions with different approaches.
There’s something for everyone, whether you’re into classic lineage tracing/fate mapping, imaging-based cell tracking, genomic barcoding, or somatic mutation tracing. Come interact with experts on these techniques, covering development, regeneration, and disease, in classical and non-classical model organisms.
Registration is free – just go to the website and request an invitation:
Simon Hippenmeyer – IST, Austria Ben Simons – Cambridge University, UK Shosei Yoshida – NIBB Okazaki, Japan Elizabeth Murchison – Cambridge University, UK Sarah Bowling – Boston Children’s Hospital -USA Bushra Raj – UPenn – USA Elke Ober- FAU Erlangen Nurnberg, Germany Trevor Graham – ICR London, UK Patrick Lemaire – CRBM, Montpellier – France Colinda Scheele – IB-KU Leuven, Belgium Michael Ratz – Karolinska Institute, Sweden Michel Cayouette – IRCM Montreal, Canada Periklis Pantazis – Imperial College London, UK Ana Cvejic – University of Copenhagen/BRIC – Denmark Marketa Kaucka – MPRGL MPI – Germany Mekayla Storer – Cambridge Stem Cell Institute, UK
Vasileios R. Ouzounidis, Mattie Green, Charlotte de Ceuninck van Capelle, Clara Gebhardt, Helena Crellin, Cameron Finlayson, Bram Prevo, Dhanya K. Cheerambathur
Kristen Kurtzeborn, Vladislav Iaroshenko, Tomáš Zárybnický, Julia Koivula, Heidi Anttonen, Darren Brigdewater, Ramaswamy Krishnan, Ping Chen, Satu Kuure
Jenifer P. Suntharalingham, Ignacio del Valle, Federica Buonocore, Sinead M. McGlacken-Byrne, Tony Brooks, Olumide K. Ogunbiyi, Danielle Liptrot, Nathan Dunton, Gaganjit K Madhan, Kate Metcalfe, Lydia Nel, Abigail R. Marshall, Miho Ishida, Neil J. Sebire, Gudrun E. Moore, Berta Crespo, Nita Solanky, Gerard S. Conway, John C. Achermann
Nikola Sekulovski, Jenna C. Wettstein, Amber E. Carleton, Lauren N. Juga, Linnea E. Taniguchi, Xiaolong Ma, Sridhar Rao, Jenna K. Schmidt, Thaddeus G. Golos, Chien-Wei Lin, Kenichiro Taniguchi
Antonia Hauth, Jasper Panten, Emma Kneuss, Christel Picard, Nicolas Servant, Isabell Rall, Yuvia A. Pérez-Rico, Lena Clerquin, Nila Servaas, Laura Villacorta, Ferris Jung, Christy Luong, Howard Y. Chang, Judith B. Zaugg, Oliver Stegle, Duncan T. Odom, Agnese Loda, Edith Heard
Federica Buonocore, Jenifer P Suntharalingham, Olumide K Ogunbiyi, Aragorn Jones, Nadjeda Moreno, Paola Niola, Tony Brooks, Nita Solanky, Mehul T. Dattani, Ignacio del Valle, John C. Achermann
Shruthi Bandyadka, Diane PV Lebo, Albert Mondragon, Sandy B Serizier, Julian Kwan, Jeanne S Peterson, Alexandra Y Chasse, Victoria Jenkins, Anoush Calikyan, Anthony Ortega, Joshua D Campbell, Andrew Emili, Kimberly McCall
Sofia E. Luna, Joab Camarena, Jessica P. Hampton, Kiran R. Majeti, Carsten T. Charlesworth, Eric Soupene, Sridhar Selvaraj, Kun Jia, Vivien A. Sheehan, M. Kyle Cromer, Matthew H. Porteus
Haitham Sobhy, Marco De Rovere, Amina Ait-Ammar, Muhammad Kashif, Clementine Wallet, Fadoua Daouad, Thomas Loustau, Carine Van Lint, Christian Schwartz, Olivier Rohr
Barbara Zhao, Jacob Socha, Andrea Toth, Sharlene Fernandes, Helen Warheit-Niemi, Brandy Ruff, Gurgit K. Khurana Hershey, Kelli L. VanDussen, Daniel Swarr, William J. Zacharias
Gerrald A. Lodewijk, Sayaka Kozuki, Clara Han, Benjamin R. Topacio, Abolfazl Zargari, Seungho Lee, Gavin Knight, Randolph Ashton, Lei S. Qi, S. Ali Shariati
Paula A. Balestrini, Abdelbaki Ahmed, McCarthy Afshan, Liani Devito, Claire E. Senner, Alice E. Chen, Prabhakaran Munusamy, Paul Blakeley, Kay Elder, Phil Snell, Leila Christie, Paul Serhal, Rabi A. Odia, Mahesh Sangrithi, Kathy K. Niakan, Norah M.E. Fogarty
Tania Hübscher, L. Francisco Lorenzo-Martín, Thomas Barthlott, Lucie Tillard, Jakob J. Langer, Paul Rouse, C. Clare Blackburn, Georg Holländer, Matthias P. Lutolf
Abdulvasey Mohammed, Benjamin D. Solomon, Priscila F. Slepicka, Kelsea M. Hubka, Hanh Dan Nguyen, Wenqing Wang, Martin Arreola, Michael G. Chavez, Christine Y. Yeh, Doo Kyung Kim, Virginia Winn, Casey A. Gifford, Veronika Kedlian, Jong-Eun Park, Georg A. Hollander, Vittorio Sebastiano, Purvesh Khatri, Sarah A. Teichmann, Andrew J. Gentles, Katja G. Weinacht
Lulu Huang, Jochem H. Bernink, Amir Giladi, Daniel Krueger, Gijs J.F. van Son, Maarten H. Geurts, Georg Busslinger, Lin Lin, Maurice Zandvliet, Peter J. Peters, Carmen Lopez-Iglesias, Christianne J. Buskens, Willem A. Bemelman, Harry Begthel, Hans Clevers
Onur Eskiocak, Saria Chowdhury, Vyom Shah, Emmanuella Nnuji-John, Charlie Chung, Jacob A. Boyer, Alexander S. Harris, Jill Habel, Michel Sadelain, Semir Beyaz, Corina Amor
Augusto Ortega Granillo, Daniel Zamora, Robert R. Schnittker, Allison R. Scott, Jonathon Russell, Carolyn E. Brewster, Eric J. Ross, Daniel A. Acheampong, Kevin Ferro, Jason A. Morrison, Boris Y. Rubinstein, Anoja G. Perera, Wei Wang, Alejandro Sánchez Alvarado
Lucia Leitner, Martina Schultheis, Franziska Hofstetter, Claudia Rudolf, Valeria Kizner, Kerstin Fiedler, Marie-Therese Konrad, Julia Höbaus, Marco Genini, Julia Kober, Elisabeth Ableitner, Teresa Gmaschitz, Diana Walder, Georg Weitzer
Qian Ma, Sijia Liu, Sara Raggi, Siamsa M. Doyle, Barbora Pařízková, Deepak Kumar Barange, Edward G. Wilkinson, Isidro Crespo Garcia, Joakim Bygdell, Gunnar Wingsle, Dirk Roeland Boer, Lucia C. Strader, Fredrik Almqvist, Ondřej Novák, Stéphanie Robert
Xiaosa Xu, Michael Passalacqua, Brian Rice, Edgar Demesa-Arevalo, Mikiko Kojima, Yumiko Takebayashi, Benjamin Harris, Hitoshi Sakakibara, Andrea Gallavotti, Jesse Gillis, David Jackson
Amir Fallahshahroudi, Leticia Rodríguez-Montes, Sara Yousefi Taemeh, Nils Trost, Memo Tellez Jr., Maeve Ballantyne, Alewo Idoko-Akoh, Lorna Taylor, Adrian Sherman, Enrico Sorato, Martin Johnsson, Margarida Cardoso Moreira, Mike J. McGrew, Henrik Kaessmann
Aurélie Hintermann, Christopher Chase Bolt, M. Brent Hawkins, Guillaume Valentin, Lucille Lopez-Delisle, Sandra Gitto, Paula Barrera Gómez, Bénédicte Mascrez, Thomas A. Mansour, Tetsuya Nakamura, Matthew P. Harris, Neil H. Shubin, Denis Duboule
Steven L. Brody, Jiehong Pan, Tao Huang, Jian Xu, Huihui Xu, Jeffrey Koenitizer, Steven K. Brennan, Rashmi Nanjundappa, Thomas G. Saba, Andrew Berical, Finn J. Hawkins, Xiangli Wang, Rui Zhang, Moe R. Mahjoub, Amjad Horani, Susan K. Dutcher
Pooja Popli, Arin K. Oestreich, Vineet K. Maurya, Marina N. Rowen, Ramya Masand, Michael J. Holtzman, Yong Zhang, John Lydon, Shizuo Akira, Kelle H. Moley, Ramakrishna Kommagani
Shruthi Bandyadka, Diane PV Lebo, Albert Mondragon, Sandy B Serizier, Julian Kwan, Jeanne S Peterson, Alexandra Y Chasse, Victoria Jenkins, Anoush Calikyan, Anthony Ortega, Joshua D Campbell, Andrew Emili, Kimberly McCall
Francisco J. Romero-Expósito, Almudena Moreno-Rivero, Marta Muñoz-Barrera, Sabas García-Sánchez, Fernando Rodríguez-Peris, Nicola Gritti, Francesca Sartor, Martha Merrow, Jeroen S. van Zon, Alejandro Mata-Cabana, María Olmedo
Enikő Lázár, Raphaël Mauron, Žaneta Andrusivová, Julia Foyer, Ludvig Larsson, Nick Shakari, Sergio Marco Salas, Sanem Sariyar, Jan N. Hansen, Marco Vicari, Paulo Czarnewski, Emelie Braun, Xiaofei Li, Olaf Bergmann, Christer Sylvén, Emma Lundberg, Sten Linnarsson, Mats Nilsson, Erik Sundström, Igor Adameyko, Joakim Lundeberg
Lara Feulner, Florian Wünnemann, Jenna Liang, Philipp Hofmann, Marc-Phillip Hitz, Denis Schapiro, Severine Leclerc, Patrick Piet van Vliet, Gregor Andelfinger
Andreana Gomez, Sergio Gonzalez, Ashwini Oke, Jiayu Luo, Johnny B. Duong, Raymond M. Esquerra, Thomas Zimmerman, Sara Capponi, Jennifer C. Fung, Todd G. Nystul
We aim to understand how human developmental cell fate decisions are controlled and exploit this knowledge for regenerative medicine and disease modelling applications. We are focusing particularly on defining how embryonic cells acquire an anteroposterior (A-P) axial identity and its effect on disease vulnerability and developmental potency/plasticity. To address our questions, we employ human pluripotent stem cell (hPSCs) differentiation as an in vitro model of early human embryonic development and utilise protocols we have recently established toward the production of human spinal cord progenitors and neural crest cells that correspond to distinct axial levels.
Current projects in our lab include:
1) Understanding how various cellular components of the trunk (e.g. motor neurons, paraxial mesoderm, trunk neural crest) are derived from multipotent axial progenitors.
2) Defining the molecular/signalling basis of A-P axial identity acquisition/maintenance in distinct neural cell types.
3) Deciphering the molecular/signalling logic of cell fate decisions in vagal neural crest derivatives focusing on the enteric nervous system and the treatment of enteric neuropathies such as Hirschsprung disease.
4) Determining how abnormal trunk neural crest development is linked to the initiation of neuroblastoma, the most common extra-cranial solid tumour of childhood.
Lab roll call
James Birch: PhD student, studying the links between cell competition and neuroblastoma initiation.
Katy Boswell: PhD student, investigating the effect of chromosome 17q gain/MYCN overactivation on the enhancer landscape of hPSC-derived trunk neural crest cells.
Fay Cooper: Postdoc, studying determinants of axial identity in spinal cord progenitors and neural crest cells. She has also been involved in the development of a hPSC-based cell therapy for treating Hirschsprung disease. More recently, Fay has been examining the effect of DNA damage on trunk neural crest differentiation in hPSCs harbouring various neuroblastoma-associated aberrations.
Grace Gilbert: Joint PhD student (primary supervisor: Dr Dan Bose), dissecting the effect of Rubinstein Taybi Syndrome disease mutations on the epigenetics of neural crest/neuronal development.
Sude Uyulgan: PhD student, working on the study of axial identity acquisition by hPSC-derived lateral plate mesoderm and limb progenitors.
Favourite technique, and why?
Anestis: Immunostaining/fluorescence microscopy analysis of neuronal projections simply because it usually produces images of immense hypnotic beauty.
Apart from your own research, what are you most excited about in developmental and stem cell biology?
Anestis: I am very excited about the increasing number of complementary 2 -and 3-D hPSC differentiation models, which together with emerging cutting-edge technologies in microscopy, sequencing and bioengineering offer for the first-time unique opportunities for controlling and understanding human developmental processes. They can also facilitate examining older classic embryology experiments/ concepts under a new lens. I am very keen on using these approaches in the near future to address specifically our group’s questions.
What is the best thing about where you work?
Anestis: The University of Sheffield hosts a vibrant developmental/stem cell biology community, and I have met some fantastic colleagues, collaborators and friends here.
Sude: Supportive and friendly atmosphere, exciting research, and most importantly having Anestis as a supervisor!
James: The University of Sheffield is a great place to work. We have regular fascinating seminars and the university has many support groups from research on aging to data analysis (Sheffield R Users Group) to support you on your research journey. The university really cares what we think, I’ve seen management respond to student and staff surveys and to really be willing to put funding behind making changes where needed. I think all these contribute to making Sheffield an exciting and supportive working environment.
Fay: The people…. we have a really great dynamic in the lab at the moment which makes it a really enjoyable place to work.
What’s there to do outside of the lab?
Anestis: Sheffield is home to lots of pubs/breweries (it is currently the real ale capital of the world), restaurants, cinemas and concert venues so there are plenty of options. It is also close to the Peak District so stunning nature/landscapes are literally around the corner.
Sude: Hiking in the Peak District, great pie and good pubs.
James: Sheffield is a really vibrant and active city with loads to do. If you like music and street food we have loads of great music venues, pubs, clubs and food stalls all around the city. We’re also just a 20 minute drive from the peaks. One of my favourite hiking areas is the Edale valley, easily accessible by car and train. There are several great hiking routes and some of the highest peaks in the peak district, as well as a cozy pub (The Old Nags Head) for a drink and a great meal after a good walk.
Fay: Sheffield is the outdoor city… it is so close to the peak district which has some amazing walking, running and swimming spots. I spend as much time as I can enjoying this!
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