A few weeks ago we interviewed Aditya Saxena, who won the poster prize at the BSDB meeting. His prize was to travel to Mexico to attend the ISDB meeting. Continuing the interview chain, Aditya interviewed Kara Nordin, who won the SDB poster prize there. As a prize, Kara will be travelling to the UK in the Spring to attend the BSDB meeting and interview the next winner.

AS: Kara, congratulations. That is fantastic. Are you happy?

KN: I am very happy. I am very excited.

AS: Where do you work?

KN: I work in Carole LaBonne’s lab at Northwestern University, in Evanston Illinois, where we study neural crest development using Xenopus laevis as our model organism.

AS: Which year of your PhD are you in?

KN: I am finishing up my fifth year of graduate study.

AS: What was your poster about?

KN: I’m investigating a group of transcription factors called SoxE factors that are essential for neural crest development. Initially, they keep neural crest cells in a stem cell state, but later on direct formation of chondrocytes, melanocytes and glial cells. We are looking into how a related Sox protein, called Sox5, modulates SoxE function in a context specific manner and additionally has roles independent of SoxE function in setting up patterning in the early ectoderm.

AS: And what did you find?

KN: I have found that Sox5 inhibits the ability of SoxE factors to promote melanocyte development, whereas Sox5 promotes the ability of SoxE factors to promote chondrocyte development. Interestingly, the ability of Sox5 to bind DNA is important for inhibition of melanocyte specific genes, whereas the ability of Sox5 to dimerize is important for promotion of chondrocytic fates.

I also have determined that Sox5 has an even earlier role, independent of SoxE factors, during ectodermal patterning. We are looking into the mechanism behind how Sox5 affects the ectoderm, possibly through BMP signaling and how this may further regulate neural crest development.

AS: So you think there is cross-talk between Sox5 and BMP?

KN: That is one of the things we are looking into right now. We think it has a role in early ectodermal patterning, and we are trying to figure out how it causes the ectoderm to be properly patterned to be competent to form neural crest forming regions.

AS: And do you know the downstream targets that Sox5 actually controls?

KN: This is one of the things that we are currently working on. We would like to determine whether or not Sox5 directly regulates downstream targets of ectodermal patterning, for instance in epidermal and neural plate fated cells. We do know direct targets later on during neural crest cell differentiation stages, including Col2a1 and Dct, where Sox5 can regulate SoxE activation of these targets.

AS: So what are your plans for this project in the future?

KN: Right now, I am trying to nail down the mechanism behind how Sox5 is functioning to pattern the ectoderm. Because we are seeing such dramatic affects on ectodermal derivatives including the epidermis, neural plate, and the neural plate border forming regions we suspect it is playing a major role in an upstream signaling pathway.

AS: It sounds really complicated, the cross talks and the transcription factors that control it. Did you hit a point when you couldn’t go any further?

KN: It is surprising sometimes to see what different directions your project will take you. When we first looked at the in situ expression profile and found that it was expressed earlier in places outside of neural crest forming regions, that intrigued us. So we looked upstream of neural crest development, looking at how Sox5 may be also involved in setting up ectodermal patterning. We think it may be that Sox5 is involved in setting up the ectoderm to allow it to become competent to form neural crest tissue.

AS: How long do you have left of your PhD?

KN: I think a couple of years. I am submitting my first manuscript on Sox5 as a regulator of ectodermal patterning, as well as also trying to put together a second manuscript on Sox5’s roles to modulate SoxE function. I am also starting to think about where I would like to do my postdoctoral work.

AS: Do you think you will stay with neural crest cells?

KN: I really love studying neural crest development, but I also want to broaden my horizons, so I am also looking at other things. But the neural crest is really a great population of cells to study. It has given me the opportunity to try different techniques, and in particular a lot of biochemical experiments. It is a really a great system to develop a good foundation in experimental biology.

AS: Is this your first prize at a SDB meeting? Have you won prizes before?

KN: It is my first prize at SDB! Last year I attended the Xenopus meeting in France and I won a poster prize there as well.

AS: And are you looking forward to going to the BSDB meeting? Have you been to England before?

KN: I have never been to England before, so I am very excited. It will be a lot of fun.

AS: And maybe you will have more data to show then…

KN: Hopefully yes. By then I hope to have nailed down the mechanism and understand molecularly what is really happening.

AS: Congratulations once again

KN: Thank you very much

(5 votes)

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SourceURL:file://localhost/Users/masa/Desktop/postdoc%20advert/Tada&Martin_Advert.doc

Two Cancer Research-UK funded research associate positions, one based at UCL (Tada Lab) and the other at the University of Bristol (Martin Lab), are available from 1-September-2013, to study the earliest interactions of host with pre-neoplastic cells in Zebrafish larva.  In particular we are interested in figuring out how epithelia can extrude pre-neoplastic cells and how the host innate immune system interacts in positive and negative ways with these cells (see Feng et al PLoS Biol, 2010;Current Biology, 2012 and Kajita et al. J Cell Sci, 2010). The post-holders will be involved in live imaging and genetic studies of these episodes at early stages of cancer initiation with a view to uncovering mechanisms which might guide development of cancer preventatives/therapeutics. Applicants should hold, or be about to obtain, a PhD in Developmental Biology/Cell Biology/Genetics, preferably with previous Zebrafish experience and with an aptitude for live cell imaging.

The positions are for 3 years for the first instance with a potential two-years extension.

For application details, please see either UCL (https://atsv7.wcn.co.uk/search_engine/jobs.cgi?owner=5041178&ownertype=fair&jcode=1326888) or Bristol (http://www.bristol.ac.uk/jobs/find/details.html?nPostingID=1009&nPostingTargetID=3245&option=28&sort=DESC&respnr=1&ID=Q50FK026203F3VBQBV7V77V83&JobNum=acad100378&Resultsperpage=10&lg=UK&mask=uobext)

Applications must be submitted by 16:00 on the closing date of 23-July-2013 (Bristol) or 1-August-2013 (UCL).

Interviews will be held jointly: one in London and the other in Bristol in mid August.

For informal enquiries, please contact Dr Masa Tada (mtada@ucl.ac.uk) or Prof Paul Martin (paul.martin@bristol.ac.uk).

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Alfonso Martinez Arias (http://bit.ly/14zBcke)

An EMBO workshop organized by Alex Schier and James Briscoe, assembled a cast of young and seasoned biologists in Oxford to discuss progress and controversies on Morphogen Gradients, a central topic in Developmental Biology. I could only stay for half of the meeting but the message was clear: there is a new era in Developmental Biology. We could call it Systems Developmental Biology or, if you do not like the association with this vague word Systems, call it Quantitative Developmental Biology. IN any event, a change in the way problems are addressed was clear: measurement, quantification, modelling and more important, analytical approaches and a critical eye on the problem were central to each and every talk. You may want to say that this is a credit to the organizers, which it is, but it is also the fact that this is the new way of an interesting field and the way forward. Developmental Biology is coming of age. This is not the place to go through the customary list of speakers and talks that made up the meeting; you can get a glimpse of this in the website (http://events.embo.org/13-morphogen/). In any case, it was the general tone and drift that caught my eye.

The notion of ‘Morphogen’ was introduced by Alan M Turing in 1952 to consider the potential of diffusible chemicals (Morphogens) to generate spatial and temporal patterns through chemical reactions. In the 70s Lewis Wolpert and his disciples brought together Gradients and Morphogens in a much more biological context, that of the generation of specific patterns, and in doing so ushered an important new era in Developmental Biology. Central to the wolpertian view was the notion of Positional Information: that there were molecules which would diffuse from a source, generate gradients and elicit specific responses in a concentration dependent manner; cell would respond to a given concentration according to its position. The gradients became “gradients of Morphogens’ and were said to be Universal. The response was more flexible and was a cell type specific. The blend of Positional Information and Morphogen gradients placed itself at the center of pattern formation and allowed a conceptualization of developmental biology in terms of signals, responses, fields, scaling that is still with us. But there was a problem, the theoretical framework lacked data.

Jeremy Gunawardena has written recently on the role of theory in Biology (1, 2) and while he chose transition states in enzyme kinetics and the gene as his examples –their notions helped Biology well before they were identified experimentally- I would add “Morphogens’ as a third theoretical entity that helped the growth, in this case of developmental biology. By the late 70s the concept of Morphogen gradients was THE conceptual reference to deal with problems in pattern formation but………. there were no Morphogens. Those with long memories will remember the rise and fall of cAMP and DIF in Dictyostelium and of Retinoic acid in the specification of digits in the vertebrate limb, and how we had to adjust our lectures through the 80s as experiments and new findings wiped the slate clean on the role of these molecules. But finally, two molecules broke in and established themselves as bona fide Morphogens: Activin in Xenopus and Bicoid in Drosophila. To do this Activin, used a very traditional blend of Biochemistry and embryology, whereas Bicoid came through the route of Genetics. It was the Genetics that would become the tool of choice in Developmental Biology and that was going to reel in other candidates; the reason for this was and is that some of these substances are in concentrations which elude the detection thresholds of the biochemical techniques but the loss of function approached of Genetics do not have this limitation.

The 90s was a good time for Morphogen gradients as genes were identified encoding molecules that, with some leniency, would fulfil the wolpertian view; in particular the criteria of inducing patterns in a concentration dependent manner. A sense of triumph was in the air. Intriguingly, and this has been a sin of the genetic approaches to developmental biology, in the euphoria we forgot some of the basis of Morphogen gradients. What is the shape of the gradients and what consequences this would have for their interpretation? If they were based on diffusion, how reproducible are they? What is the role that time, the dynamics of gradient formation and read out, played in the development of pattern? How robust is the response? The theory of Morphogen gradients created a quantitative framework that needed to be explored. Moreover, Wolpert had been explicit in addressing the problem of pattern scaling, which needed to be related to the mechanism of Morphogen activity. These issues which, for the most part have been swept under the carpet, were at the center of the EMBO meeting.

In the early 00s, three laboratories, all of them working with Drosophila, rose to the challenge of dealing with the real questions of Morphogen gradients. They did so by blending physical approaches with an extension of the potential of genetics beyond the identification of genes challenge. John Reinitz and Eric Wieschaus in the US tackled the early pattern formation of Drosophila embryos and Marcos Gonzalez Gaitan, addressed the issue of the presumed gradients of Dpp in the wing imaginal discs and its consequences. This work was at first seen as esoteric, but in the course of time has established standards, reformulating questions and generating new perspectives on old problems; in the process, it has revealed some surprises. Many other people have followed and the meeting in Oxford was a lively exponent that these arguments are now at the heart of the discussions.

If the gradients required detailed interrogation and analysis, the response of the cells is in need of no lesser scrutinity. Wolpertian gradients posed linear gradients and linear interpretations but biological systems are different. Linear approximations might work in some instances but the response, particularly when signal transduction is in the middle, is more complicated. Here the work of James Briscoe (one of the organizers) on the translation of the continuous gradient of Sonic Hedgehog into discrete states in the vertebrate neural tube, paves the way for progress. Gradients need to be decoded and time, as well as the traditional spatial variable, plays an important role. On the side of the interpretation, more classical molecular biology techniques are important and the development of genomics is central to understanding this important part of the action of Morphogen gradients and the programme of the second day, which I had to miss, had much bearing on this. Networks and models linking patterns and genes are the elements of this analysis. Most importantly, as has been emphasized by Arthur Lander, there has to be a feedback between the input and the output and we are far from undertanding it. Early days, but moving forward.

Whereto Genetics in this new era? I have expressed my thoughts elsewhere (http://bit.ly/10fZkmK) but the meeting made one thing clear: Genetics has been useful to break the system into component parts and to identify those parts, but it is not the tool of choice to put the system back together; it cannot be. Genetics still works well to identify components but in the new era, it is a perturbation tool to challenge the system out of its comfort zone (the one chosen by Evolution) to test some of the predictions of the models. This much was also clear, though perhaps not explicit, at the meeting.

With a few exceptions, every talk I sat through in Oxford was quantitative, analytical and critical of itself and of the field. While there were glimpses of solutions to particular problems, there was a general feeling that there is a way to go in some of the important questions and this critical look at the problems is, it seems to me, a good thing. What we have in front of us, whether Morphogen gradients or decision making during development, are formidable tasks which cannot be ‘solved’ by joining gene names with arrows or drawing linear gradients that outline French flags (even Lewis Wolpert admits this). Over the last few years interactions with the Physical Sciences are changing Biology. The result goes beyond Biophysics, while this looks at Biology for problems that look like Physics, the new interactions looks at physics for inspiration and methods to address biological problems which, often, physics has never encountered. The benefits of this new approach were in display at the workshop in Oxford and make us look forward to a future that will not only give us more a satisfactory understanding of the system at the mechanical level, but also will begin a more mature and quantitative biology. After all, if there is a frustration in Biology (and this was an undercurrent of the meeting and where it differs from Physics) is that Natural Selection has ensured that there is fine tuning at all levels of description, from the levels and timing of the expression of a gene, to the scaling and dimensionality of tissues and organs. Such a system will require much that is new in terms of concepts and techniques. What a formidable challenges but also, what fun!

References
1. Gunawardena J. (2012) Some lessons about models from Michaelis Menten. Mol. Biol. Cell 23, 517-519.

2. Gunawardena J. (2013) Biology is more theoretical than physics. Mol. Biol.Cell 24, 1827-1829.

(7 votes)

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Olivier Pourquie, Nipan Patel, Claudio Stern, John Wallingford, Mary Mullins, Alejandro Sanchez-Alvarado, Andrea Streit, among other will teach, hands-on, the paradigms, problems and technologies of modern Developmental Biology. The course will start with a plenary lecture by Scott Gilbert about the history and concepts in Developmental Biology.

The course will take place between 5th and 17th January 2014 in Quintay-Chile.  Full fellowships will be available for Latin American students.

New Fellowships for non-Latin American students are offered by the Society of Developmental Biology (SDB) and University College London (UCL). The fellowships will cover the full course fees and travel expenses.  For more information about the fellowships go to:

http://biodesarrollo.unab.cl/fellowships-costs

DEADLINE 31st July 2013

For a summary about the previous course see:

https://thenode.biologists.com/a-wave-from-quintay-2/

Picture of previous course is shown below, with  John Gurdon interacting with the students:

(1 votes)
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Next week will see the 8th european zebrafish meeting happening in Barcelona, Spain. The Node will be there, and (if there is internet available) we will be tweeting from the conference using the hashtag #ezfish2013. So follow us on Twitter to find out what is happening! In addition, we have a group of Node zebrafish bloggers who will be reporting from the conference, so expect their meeting reports here on the Node.

If you are attending the conference, do look out for Cat. The Node will not have a stall, but Cat will be around, and is really looking forward to meet many zebrafish developmental biologists! You can see what Cat looks like in her introductory post. Looking forward to meet you there!

(4 votes)

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One of my favourite radio programs on the BBC is called Desert Island Discs. In this programme, a politician, activist, actor, writer or scientist is asked: if you had to live in a desert island, what would be the 8 records/songs that you would take with you? Although it is interesting to know what kind of music renowned people like, the music is really just an excuse. The reasons to choose each song are normally engrained in the interviewee’s life and achievements, and as such the music is just the starting point to a very personal and interesting interview. Desert Island Discs has been broadcast since 1942, and a vast proportion of the archive is available online to browse and enjoy. While perusing, I found that not only many known scientists have been interviewed, but a selection of those are well known developmental biologists. Here are some of the highlights:

– Martin Evans, Nobel Prize winner for his pioneering work on isolating and analysing embryonic stem cells

– John Sulston, Nobel Prize winner for his work on lineage analysis and programmed cell death in C. elegans

– Lewis Wolpert, known for his work on positional information in developmental biology, and for proposing the ‘french flag’ model of morphogen gradients.

If you want to know a little about the lives and experiences of these developmental biologists, then why not listen to the interviews? Many other scientists have been interviewed over the years (as well as many other interesting personalities) and you can access and search the full archive here.

(6 votes)

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Here are the highlights from the latest issue of Development.

Extrinsic cue for dendrite polarisation

Most neurons have a single axon on one side of their cell body and multiple dendrites on the opposite side. The establishment of this polarisation, which is essential for neuronal function, probably involves both intrinsic and extrinsic factors. Although several intrinsic factors have been identified, the identity of the in vivo extrinsic signals remains unclear. To remedy this situation, Sarah McFarlane and co-workers (p. 2933) have been studying dendrite polarisation in Xenopus retinal ganglion cells (RGCs). They report that neuropilin-1 and plexinA1, which form a holoreceptor for members of the axon guidance family of class III secreted semaphorins (Sema3s), are necessary to bias dendrite extension to the apical side of RGCs in vivo. They report that sema3a and sema3f are expressed on the basal and apical sides of the Xenopus RGC, respectively. Moreover, ectopically expressed Sema3s and inhibition of receptor signalling disrupt dendrite polarisation. The researchers suggest that neuropilin-1 and plexinA1 are co-receptors for an extrinsic cue, probably a Sema3, that directs RGC dendrite polarisation independent of axon polarisation.

Bi-polarity in tubulogenesis

Apico-basal polarisation is a crucial step in the formation of biological tubes. In model systems in which tubulogenesis occurs in cell clusters, the inner surface of each cell in the cluster differentiates into an apical domain where lumen secretion occurs, thus ensuring the formation of an unobstructed lumen. But in many developmental contexts, tubes form from simple cords of cells, which presents a unique challenge for the formation of a continuous lumen. On p. 2985, Di Jiang and colleagues investigate how this challenge is overcome during tubulogenesis in the Ciona intestinalis notochord, which is made up of a single file of geometrically identical cells. The researchers show that, during early tubulogenesis, a patch that contains the highly conserved Par complex and a set of tight junctions becomes established at both ends of the notochord cells. The formation of these two apical domains, they report, is controlled by Par3. Together, these results suggest a new mechanism for tubulogenesis from a simple cell cord that requires the formation of bi-apical cells.

Plants and animals converge to imprint

In mammals and plants, parental genomic imprinting, which results from mitotically stable epigenetic modifications, restricts the expression of specific loci to one parental allele. During gametogenesis in mammals, imprinting involves sex-dependent de novo DNA methylation and non-coding RNAs but does a comparable mechanism operate in plants? Here (p. 2953), Thiet Minh Vu, Frédéric Berger and colleagues report that de novo RNA-directed DNA methylation (RdDM), which depends on small interfering RNAs, regulates imprinting at several loci in Arabidopsis endosperm. By dissecting the expression of various members of the RdDM pathway, the researchers show that RdDM is required in somatic tissues to silence both parental alleles, whereas repression of RdDM in female gametes contributes to the activation of the maternal allele. Hence, both de novo DNA methylation and non-coding RNAs play a role in the regulation of imprinted loci in plants and mammals, which suggests that convergent evolutionary processes contribute to imprinting in these distinct groups of eukaryotes.

Rubbing out epigenetic marks in PGCs

During the migration of primordial germ cells (PGCs) to the genital ridge and during gonadal development, the stepwise erasure of DNA methylation and histone dimethylation marks ensures PGC totipotency and prevents the accumulation of epimutations. On p. 2892, Yoshiyuki Seki and co-workers explore the mechanisms underlying genome-wide epigenetic reprogramming in mouse PGCs by investigating the dynamics of epigenetic modifications in transposable elements. CpG methylation is markedly decreased in short interspersed nuclear elements (SINEs) in migrating PGCs, they report, but not in long interspersed nuclear elements (LINEs). By contrast, CpGs are rapidly demethylated in both SINEs and LINEs in gonadal PGCs. Four major factors that maintain DNA and histone methylation during DNA replication (and whose inhibition is associated with replication-dependent passive demethylation) are repressed at distinct stages of PGC development, they report, and DNA demethylation of transposable elements is disturbed in PGCs in which proliferation is impaired. These and other results suggest that PGCs use both active enzyme-catalysed DNA demethylation and passive demethylation for genome-wide epigenetic reprogramming.

Wnt signalling in early embryos

The Wnt signalling pathway is clearly required for gastrulation in mammalian embryos, but little is known about its extra-embryonic and preimplantation functions. Here (p. 2961), Janet Rossant and co-workers investigate the requirements for Wnt signalling in early mouse development using a mouse line that carries a floxed allele for the porcupine homolog (Porcn) gene. Porcn is required for the acylation and secretion of all 19 mammalian Wnt ligands, so Porcn function represents a bottleneck for Wnt signalling. Using zygotic, oocyte-specific and visceral endoderm-specific deletions of Porcn, the researchers show that Porcn-dependent Wnt signalling is not required for preimplantation development or for implantation itself, and they confirm that gastrulation is the first Porcn/Wnt-dependent event in embryonic tissues. They also identify chorio-allantoic fusion as the first major Porcn/Wnt-dependent event in extra-embryonic tissues. Together, these findings show that, although Porcn-dependent Wnt signalling is important for embryonic and placental function, it does not have an essential role in preimplantation development or in blastocyst lineage specification.

Gpr125 helps set gastrulation in motion

During vertebrate gastrulation, polarised cell behaviours orchestrated by Wnt/planar cell polarity (PCP) signalling drive the convergence and extension (C&E) movements that elongate the embryo. Xin Li, Florence Marlow, Lilianna Solnica-Krezel and colleagues now identify Gpr125, an adhesion G protein-coupled receptor, as a novel modulator of Wnt/PCP signalling during gastrulation in zebrafish embryos (p. 3028). The researchers show that overexpression of Gpr125 impairs C&E movements in zebrafish embryos and that reduced Gpr125 function exacerbates the C&E defects and the facial branchiomotor neuron migration defects seen in embryos with reduced Wnt/PCP signalling. Gpr125 directly interacts with Dishevelled (Dvl), they report, and recruits Dvl to the cell membrane, a prerequisite for Wnt/PCP activation. Finally, they show that Gpr125 and Dvl mutually redistribute into discrete membrane subdomains and recruit a subset of PCP components into membrane subdomains. Thus, the researchers suggest, Gpr125 might act as a component of PCP membrane complexes and as a modulator of Wnt/PCP signalling in vertebrates.

Plus…

Tubulogenesis

In this  poster, Luisa Iruela-Arispe and Greg Beitel summarise our current understanding of the various processes by which tubes form during development, and the cellular and molecular mechanisms underlying tubulogenesis.

See the Development at a Glance article on p. 2851

Oct transcription factors in development and stem cells: insights and mechanisms

Oct proteins play varied and essential roles during development. Here, Dean Tantin outlines our current understanding of Oct proteins and the regulatory mechanisms that govern their role.

See the Primer article on p. 2857

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Developmental biology from near the south pole

Kia Ora from New Zealand

Hi, I’m Megan Wilson and I’m a lecturer in the Department of Anatomy at the University of Otago, Dunedin, New Zealand. My research focus, and my scientific passion, is developmental biology. In this new regular blog on the Node, I hope to bring NZ developmental biology to the rest of the world. The NZ developmental biology community is vibrant and diverse, and overlaps with a range of other disciplines, from medical research to evolution and ecology.

It wasn’t always the case, though.  As a Biochemistry undergrad, and then a PhD student at Otago in the late 1990s, there were very few options for studying developmental biology. My interest in developmental biology came from wanting to know more about the genetic disorder my brother had, Tuberous Sclerosis Complex (TSC). TSC affects multiple organ systems, but particularly the kidney, brain and skin, causing benign tumors to grow.  I wondered why these tumors occurred in only a few organs and why symptoms varied so much between individuals.  In order to really understand the origin of this disorder, I had to learn a little developmental biology, became fascinated by it, and this sparked a career shift.  After a postdoc with Prof Peter Koopman in the Institute for Molecular Bioscience in Brisbane, I returned to Otago to work as a Research Fellow with A/Prof Peter Dearden in Biochemistry, before establishing my own lab in the Anatomy Department at Otago in 2010.

Otago University is located in the lower half of the South Island in Dunedin, New Zealand.  It was the first University in New Zealand (founded in 1869) and now boasts a student population of over 20,000 and teaching/research campuses in Wellington and Christchurch as well as Dunedin.  It is very much a ‘research-led’ institution with most teaching academics also running active research groups. Developmental biology at Otago is by nature multidisciplinary and multidepartmental –  researchers from the departments of Anatomy, Biochemistry, Medicine, Zoology and Pathlogy contribute to teaching popular 3rd and 4th year courses on development.  At Otago there has a been a steady growth in developmental biology research over the past 10-15 years, to the point where today there are more than 10 independent research labs at Otago University, including my own http://wilsonlab.otago.ac.nz.  Developmental biology research at Otago covers a full range of animal and plant systems including mammals, insects, amphibians, urochordates, Arabidopsis and humans.

Why post about NZ developmental biology research?

Well, while NZ is a wonderful place to live and we have all the tools and talent to perform cutting-edge research, it’s true that we are very very far from everyone else.  To get to a conference in the US or Europe, requires up to 36 hours in a plane (or more often many planes) and several thousand dollars in travel funds. As a result, many of us only get to one international meeting a year, and very rarely to smaller more focused meetings.  This makes it very difficult to network, set up new collaborations or present our science to a wider audience.  So the next best thing is to make use of social media such as blogging to share with everyone some of the exciting work being carried out in New Zealand, which is what I hope to do here.

So in coming posts here at the Node I will profile some of the leading researchers at Otago and elsewhere in New Zealand.  I will also provide meeting reports from local conferences from this side of the world. If you have any thoughts or comments, email me on meganj.wilson@otago.ac.nz.

(13 votes)

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June saw a lot of interesting posts on the Node! Meeting reports, research posts, and more, as well as few new jobs in our jobs page. Here are some of the highlights:

Meeting reports

Several meetings were covered on the Node this month:

• – Harry attended the International Society for Stem Cell Research (ISSCR) annual meeting in Boston, and posted a series of reports on the meeting
• – The Node was at the International Society of Development Biology (ISDB) in Cancun, and posted daily updates.
• – Rachael attended the satellite symposium on left-right asymmetry that preceded the ISDB, and wrote a post on her highlights.

Research Highlights

– Kif Liakath-Ali highlighted two recent papers that used insects as an inspiration to develop new technologies.

– Cantas discussed his recent paper on the formation of the primitive streak and the induction of mesoderm.

– And Albert described how he applied the brainbow technique to zebrafish.

Woods Hole embryology course

This year’s Woods Hole embryology course is underway, and Lara wrote a post about her impressions on the first few weeks of the course. We also had another round of beautiful images from last year’s course up for voting, and the big winner this time was a skeleton preparation of a pig embryo. This winning image will feature in the cover of Development in a coming issue.

Also on the Node: 

– Kara wrote a personal account of how she returned to the bench after working as a journal editor, showing that leaving academia doesn’t necessarily mean that you can’t come back.

– And Erin’s stem cell image blog focused on a recent paper on retinal regeneration.

Happy Reading!

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Just before the ISDB meeting in Mexico, over a hundred researchers gathered for a satellite symposium on the development of left-right asymmetry. Although the external body plans of vertebrates (and many invertebrates) are bilaterally symmetrical, various internal organs are positioned asymmetrically. For example, the heart is located towards the left, but paired organs such as the lungs are also asymmetrical, as the left lung has fewer lobes than the right in order to make space for the heart on that side of the body. Correct development of the left-right axis is vital for all organs to be packaged properly within the body cavity, so left-right defects often have disease implications.

The symposium began by looking at left-right patterning in some of our more distant relatives: flies, nematodes, polychaete worms, limpets, sea squirts and sea urchins.

Bill Wood used a nice visualisation to describe left-right asymmetry in the early C. elegans embryo, telling us to imagine the one-cell embryo as a cylinder with the M.C. Escher artwork ‘Bird Fish’ wrapped around it. As the embryo prepares for its first cell division into anterior and posterior cells, there is an off-axis deformation of the cortical network that stretches the birds and fish so that they are longer and thinner on one side of the cylinder. This means that the birds and fish on the other side are pulled in the opposite direction and become shorter and fatter, creating a difference between the future left and right sides of the body during the very first cell division. ‘Bird Fish’ seemed an appropriate choice of pattern to demonstrate this point as the model organisms representing these two groups, chick and zebrafish, use very different mechanisms to establish left-right asymmetry and would be discussed later in the meeting…

We then moved on to vertebrate systems with talks describing the events taking place at the node of mouse and its analogous structures in other animals, where motile cilia generate leftward fluid flow. Dominic Norris proposed a mechanism for the detection of this flow, which initiates a Nodal signal on the left side of the body, while Chris Wright and Jose Antonio Belo talked about the dynamics of Nodal ligands and antagonists, respectively.

A recurrent debate was the role of early determinants of asymmetry, and how these might work with the cilia-mediated mechanism seen in many, but not all vertebrates. Martin Blum proposed a model to accommodate both processes and discussed their possible evolutionary relationships. The frequently mentioned ‘problem’ with a cilia-based strategy is that some animals establish the left-right axis without cilia, such as the chick. Leonor Saude described the asymmetry created by the leftward movement of cells around Hensen’s node and the termination of this process by a cell adhesion mechanism.

The early differences between the left and right sides of the body must be translated into an effect on organogenesis later in development. This was addressed by Rebecca Burdine who showed that the Nodal signal increases cell movement on the left side of the zebrafish heart tube to facilitate its leftward jog, and Nanette Nascone-Yoder who has been investigating the role of the left-specific transcription factor Pitx2 in asymmetric gut curvature in frogs.

Even organs that appear symmetrical in their gross morphology can be asymmetrical; the brain exhibits many functional asymmetries. Steve Wilson has utilised the optical clarity of zebrafish to visualise asymmetric connections in the brain and asymmetric activity in response to stimuli, while Marnie Halpern described some ways in which reversed brain asymmetry can affect fish behaviour.

In contrast to the talks on asymmetry, Olivier Pourquie explained how symmetrical structures such as the somites overcome the differences between the left and right sides of the body to maintain their symmetry during development.

The medical relevance of left-right axis development was summed up by talks on diseases associated with asymmetry defects; Cecilia Lo described her work on congenital heart disease and Zhaoxia Sun spoke about primary ciliary dyskinesia. Research into left-right asymmetry has even inspired a recent article in the New York Times, so a developmental process that has fascinated scientists for decades has infiltrated popular culture too – it must be important!

(3 votes)

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