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The Inclusive STEM Teaching Project

Posted by , on 11 July 2023

The Inclusive STEM Teaching Project is a 6 week course designed to advance the awareness, self-efficacy, and the ability of faculty, postdocs, and doctoral students to cultivate inclusive STEM learning environments for all their students and to develop themselves as reflective, inclusive practitioners.

This is a free, online course (run through edX) that provides an excellent overview of inclusive STEM teaching. We highly recommend this course to all who teach or might teach.

Inclusive STEM Teaching Project Logo

The course runs from Oct 2 to Nov 21, 2023.

Register here https://www.edx.org/course/the-inclusive-stem-teaching-project.

What you’ll learn

Participants in this course will…

  • Examine issues of diversity, equity and inclusion in higher education
  • Reflect on their own and their students’ identities and experiences
  • Question their assumptions about all aspects of instruction
  • Identify and implement learner-centered structures and strategies
  • Apply principles of evidence-based inclusive teaching
  • Use their student learning data and feedback to inform pedagogical and curricular choices

———-

This article is reposted from the ROOT & SHOOT blog (https://rootandshoot.org/the-inclusive-stem-teaching-project/). ROOT & SHOOT is a NSF-funded Research Coordination Network granted to seven plant science organizations that are seeking to “Root Out Oppression Together & SHare Our Outcomes Transparently”. In other words, we recognize that science as traditionally practiced is inherently exclusionary, and we are exploring strategies to build a more inclusive scientific culture. We invite you to visit our project home page, check out our Resources page, and follow us on Twitter (@RootAndShootRCN).

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“Science and Social Media. Are they really compatible?”

Posted by , on 11 July 2023

Reflections on a social media workshop that was part of the Society for Experimental Biology Centenary Conference 2023.

The use and availability of social media platforms are constantly changing, as the recent launch of Threads by Meta Platforms and our own server on Mastodon – biologists.social – nicely exemplify. For years, Twitter has been the go-to platform for biologists to share and discuss recent scientific advances, but this may well change. Regardless of what may – or may not – happen, a refresher on the different ways to use social media to engage with other scientists (and the public) seemed timely.

At the recent SEB Centenary Conference in Edinburgh (UK), Isabel Mendoza (Global Plant Council), Geraint Parry (Global Plant Council & Association of Applied Biologists) and Mary Williams (American Society of Plant Biology) teamed up to provide a workshop with exactly this in mind. They named it: “Science and Social Media. Are they really compatible? Developing Effective Science Communication Strategies”.  

To start things off, Isabel Mendoza showed us (= the participants) some user statistics in order to rank the available social media platforms. This re-affirmed the notion that the platforms we often use as biologists don’t necessarily have the widest reach. Why not use Pinterest, for example, which may actually be a very efficient platform to reach new audiences?

When reaching out to new (and existing!) audiences, thinking about their needs is crucial. How can you make sure that you communicate in a way that is inclusive? The workshop organisers had plenty of examples – such as the lack of ALT-text, or wrong use of hashtags/emojis – that quickly make things inaccessible to people with marginalized identities. Even with all the right intentions…

The topic of inclusivity was actually discussed in the context of a larger movement that drives a cultural change in the plant sciences, known as the ROOT & SHOOT project. This 5-year project, combining the expertise of seven plant science organisations, aims to “remove barriers for individuals historically excluded from science based on gender, gender identity, disability status, sexual orientation, ethnicity, or race”. The title of the project refers to the goals to “Root Out Oppression Together and SHare Our Outcomes Transparently” (ROOT & SHOOT). A wonderful initiative that will hopefully be followed by many others within and outside the biological community.     

There wasn’t just one take-away message from this SEB workshop. Rather, there were many different things to consider. One particular consideration that struck a chord with the participants was this: if you’re still on the fence about using social media as a scientist, there is never an ideal time to join – so why not just dive in straight away? One of the workshop participants took this advice to heart and created her Twitter profile on the spot – that’s the spirit! 💪

For more detailed information about (part of) this workshop, take a look at this page created by Mary Williams: https://rootandshoot.org/how-can-social-media-to-build-a-more-inclusive-scientific-community/

Attendees drafting a Tweet about the workshop. Image taken and shared by the Global Plant Council.
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Course announcement: Gene Regulatory Networks for Development

Posted by , on 6 July 2023

After a pandemic related hiatus the MBL Gene regulatory networks (GRNs) course at Woods Hole is back. It’s been refreshed with new course directors and faculty and is better than ever. It covers experimental and computational methods used to study GRNs, through highly interactive lectures, discussions, group projects, and practical tutorials.

Course date:
Oct 08, 2023 – Oct 20, 2023

Application deadline:
July 21, 2023

To apply:
https://www.mbl.edu/education/advanced-research-training-courses/course-offerings/gene-regulatory-networks-development

Gene regulatory networks (GRNs) are key to the genomic control of development in animals and plants. To study GRNs requires insights from various research fields, including systems biology, developmental and evolutionary biology, as well as functional genomics, and provides an integrative approach to fundamental research questions in biology. This course introduces the concepts of GRNs, and teaches experimental and computational methods used to study them, through highly interactive lectures, discussions, group projects, and practical tutorials. We will cover a broad range of topics, including transcriptional control systems, the structural organization of hierarchical networks, developmental functions of GRN circuit modules, GRN evolution, and computational modelling using BioTapestry as well as Boolean and quantitative mathematical approaches. Students will learn how to generate GRN models based on data extracted from the literature, and will generate computational models to analyze dynamic circuit behavior. We will present and discuss a broad range of experimental approaches and how they are effectively used for studying gene regulation and developmental GRNs. Examples of experimentally solved developmental GRNs from a variety of organisms, such as flies, sea urchins, frogs, chicken, and mice, will be explored. Students are encouraged to share their research projects in a poster session, and to discuss with course faculty how to apply the approaches taught in the course to their own research questions. The course is intended for advanced graduate students, postdoctoral scholars, and faculty.

Course Faculty and Lecturers
Leslie Babonis, Cornell
Sarah Tulin, Canisius College
Crystal Rogers, UC Davis
Scott Barolo, University of Muchigan
Doug Erwin, Smithsonian
Mark Rebeiz, University of Pittsburgh
William Longabaugh, Institute for Systems Biology
Paola Oliveri, University College London
Roberto Feuda, University of Leicester
Nipam Patel, Marine Biological Laboratory
Zeba Wunderlich, Boston University
Hui-Chun Lu (grace), University College London
James Briscoe, Crick Institute
Andrea Streit, King’s College London
Megan Martik, UC Berkeley

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SciArt profile: Kimberley Snowden

Posted by , on 6 July 2023

In this latest SciArt profile, we spoke with Kimberley Snowden, who has a background in plant molecular and developmental biology and makes science-inspired polymer clay artwork.

Can you tell us about your background and what you work on now?

I have a background in plant molecular and developmental biology. I enjoy working out at a mechanistic level how plants get their very complex forms. In particular, I have worked on plant shape or architecture, and the molecular genetic controls of plant branching for a while now. While we understand a lot about the hormones and signals that contribute to the development of branches, there is still a lot to learn, particularly at the level of the whole plant.

Leaf cross section used on a tea-light holder. A complex polymer clay cane was made to represent the cellular organisation of a leaf, including the epidermis, mesophyll, vascular bundles and guard cells. Slices of the cane were used here to cover a tea-light holder. The air spaces of the leaf were made using translucent clay.

Were you always going to be a scientist?

Yes I think so. From a reasonably early age I enjoyed the idea that we can understand the world around us using science. That if we collected data and used some logic, then perhaps we could figure out how things work. The idea that science can help us solve problems and help advance the human race is very appealing to me. Although as I’ve gotten older I’ve come to appreciate our problems require so much more than science to give us enduring solutions.

Root tip This cane is a stylised representation of the cellular organisation of a plant root tip.

And what about art – have you always enjoyed it?

I never felt I had much aptitude for art for much of my life. During my postdoc years I lived in a few very beige apartments and became very drawn to environments and art that were full of colour. I had dabbled in pottery on and off for many years, but had been frustrated that I couldn’t get the detail or colour that I wanted to. And then by chance in 2017 I stumbled into the world of polymer clay, being captivated by the colours and possibilities of this medium. Along the way I learned cane making, and have been hooked ever since.

Meristem Cane This is a full-sized cane before reduction (approximately 12 cm wide). The subject is a plant shoot tip, with the darker cells portraying the shoot apical meristem along with two axillary meristems in the developing leaf axils. This one is probably the closest to the science I do!

What or who are your most important artistic influences?

I’ve always been drawn to tactile types of art, including ceramics, glass, and sculpture. In general I like colour and abstract art, but actually many different art styles and forms appeal to me, so it’s hard to narrow that down. In the polymer clay world I am inspired by many artists, and would suggest to those unfamiliar with this medium to check out the works of people like Kathleen Dustin, Georg Dinkel, and Adam Thomas Rees. On a more personal level, a lot of the skills I learned to make canes came from the generosity of artists like Fiona Abel-Smith.

Animal cell cane
This is a polymer clay cane portraying an animal cell. This cane has been reduced and is about 3 cm wide, while the original cane started out about 11 cm wide.

How do you make your art?

I start with a rough sketch of my subject, often based on looking at many images of whatever that might be. Sometimes I spend time learning about the subject as well, so that I feel I know a bit more than what I might see in photographs. Then I need to work out the level of detail needed, and spend time thinking about how to achieve that, or if it is achievable at all at the scale I work at. Some things that might be easy to draw can be very difficult to convert to a cane format. An example of that is when I recently made a version of Hokusai’s Great Wave off Kanagawa in cane form – there the small details of the wavelets took me a few attempts and approaches to get something I was happy with. Consideration of contrast and colour combinations is also very important, and not something I feel I have mastered yet. Then I usually build the components of the cane, incorporating blends, and a few other different methods before reducing those to a size that can be used in the cane. Assembly of the components into the cane happens next and requires components to be sized appropriately and for everything to be fitted well together. Rushing that aspect usually causes a lot of problems in the final cane, as the physics of reduction mean that clay will move into any gaps causing distortion. My complex canes are often built around 12 cm in diameter and 4 or 5 cm in height, and once finished the whole cane is reduced down to something more like 3 cm in diameter. Final items are made with slices of my canes, which are then cured by baking in a domestic oven.

Diatom picture. This one is based on a photograph by Anatoly Mikhaltsov of a fossil diatom from New Zealand.
Going viral. While in lockdown in 2020, I made a cane that was my version of a SARS-CoV-2 virus. A couple of years later I used slices to assemble this piece.

Does your art influence your science at all, or are they separate worlds?

I think it is more that my science influences my art. My science background has taught me to be detail oriented which often results in me putting too much detail into my art. I have to balance portraying what is real versus what I think could convey the essence of a subject. An example of that would be my animal cell cane. There are no animal cells that look exactly like that, and the scale of different organelles is all over the place, which was necessary so that they could still be recognisable in the finished piece. In spite of that most people would still recognise it as being a cell.

What are you thinking of working on next?

I haven’t decided, and I’m always open to ideas! A few of my science canes have come from suggestions by other scientists. Some science subjects don’t lend themselves easily to a cane design, and so I sometimes think about a possible subject a long time before I start on it. Currently I have another native New Zealand flower in mind as one possibility. In the meantime, I often make kaleidoscope canes when I’m between complex cane projects, which I enjoy because of the combination of symmetry and often quite organic designs.

Native birds and plants of New Zealand. These small artworks are each approximately 10 cm square. They are made entirely of polymer clay (except a small bit of embedded wire for hanging). They each show a native New Zealand bird with a background appropriate to where these birds are found. Top left is a kererū (native wood pigeon) with a background of ngutu kākā flowers and foliage. Top right shows a pīwakawaka (also known as a fantail) with a background of kōpukapuka (also known as Chathan Islands forget-me-not, although it is not a true forget-me-not). On the bottom left is a kea (a very smart alpine parrot), with a more abstract background (working with alpine colours in a kaleidoscope format), and on the bottom right is a tūī with a background of kōwhai flowers and foliage. In the wild our kea, ngutu kākā and kōpukapuka are threatened from a conservation stand-point.
Making of a complex cane. This montage shows a few of the steps involved in making a complex polymer clay cane. The top left panel shows the beginnings of the wave construction, the top middle shows construction about half way through and the top right shows the completed cane at full size (approximately 11 cm in diameter). On the middle right, you can see the cane during reduction, and the bottom right image shows the completed cane with the interior showing (here the cane is approximately 3 cm in diameter). The large image on the left shows a completed pin constructed from a slice of this cane.

To find out more about Kim’s artwork:

Instagram: @KimSnowden7

Twitter @KimSnowden12

Etsy: KimSnowdenDesigns

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June in preprints

Posted by , on 5 July 2023

Welcome to our monthly trawl for developmental and stem cell biology (and related) preprints.

The preprints this month are hosted on bioRxiv and aRxiv – use these links below to get to the section you want:

Developmental biology

Cell Biology

Modelling

Tools & Resources

Developmental biology

| Patterning & signalling

Recording morphogen signals reveals origins of gastruloid symmetry breaking

Harold M. McNamara, Sabrina C. Solley, Britt Adamson, Michelle M. Chan, Jared E. Toettcher

Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration

Alessandra M. Norris, Ambili Bai Appu, Connor D. Johnson, Lylybell Y. Zhou, David W. McKellar, Marie-Ange Renault, David Hammers, Benjamin D. Cosgrove, Daniel Kopinke

Negative feedback on Retinoic Acid by Brachyury guides gastruloid symmetry-breaking

Meagan J. Hennessy, Timothy Fulton, David A. Turner, Ben Steventon

Growth plate resting zone chondrocytes acquire transient clonal competency upon Hedgehog activation and efficiently transform into trabecular bone osteoblasts

Shion Orikasa, Yuki Matsushita, Michael Fogge, Koji Mizuhashi, Naoko Sakagami, Wanida Ono, Noriaki Ono

Robust axis elongation by Nodal-dependent restriction of BMP signaling

Alexandra Schauer, Kornelija Pranjic-Ferscha, Robert Hauschild, Carl-Philipp Heisenberg

Zebrafish blastoderm explants from Schauer et al. The image is made available under a CC-BY-NC 4.0 International license.

Temporally resolved early BMP-driven transcriptional cascade during human amnion specification

Nikola Sekulovski, Jenna C. Wettstein, Amber E. Carleton, Linnea E. Taniguchi, Xiaolong Ma, Sridhar Rao, Jenna K. Schmidt, Thaddeus G. Golos, Chien-Wei Lin, Kenichiro Taniguchi

Specification and survival of post-metamorphic branchiomeric neurons in the hindbrain of a non-vertebrate chordate

Eduardo D. Gigante, Katarzyna M. Piekarz, Alexandra Gurgis, Leslie Cohen, Florian Razy-Krajka, Sydney Popsuj, Hussan S. Ali, Shruthi Mohana Sundaram, Alberto Stolfi

Crosstalk between glucose metabolism and morphogen signalling specifies tonotopic identity in developing hair cells

James DB O’Sullivan, Thomas S Blacker, Claire Scott, Weise Chang, Mohi Ahmed, Val Yianni, Zoe F Mann

Rewiring of the epigenome and chromatin architecture by retinoic acid signaling during zebrafish embryonic development

Marta Moreno-Oñate, Lourdes Gallardo-Fuentes, Pedro M. Martínez-García, Silvia Naranjo, Sandra Jiménez-Gancedo, José L. Gómez-Skarmeta, Juan J. Tena, José M. Santos-Pereira

Mutation in the Bone Morphogenetic Protein signaling pathway sensitize zebrafish and human to ethanol-induced jaw malformations

John R Klem, Tae-Hwi Schwantes-An, Marco Abreu, Michael Suttie, Raeden Gray, Hieu Vo, Grace Conley, Tatiana M Foroud, Leah Wetherill, CIFASD, charles B lovely

Clonal neural tube organoids self-organise floorplate through BMP-mediated cluster competition

Teresa Krammer, Hannah T. Stuart, Elena Gromberg, Keisuke Ishihara, Manuela Melchionda, Jingkui Wang, Elena Costantini, Stefanie Lehr, Dillon Cislo, Laura Arbanas, Alexandra Hörmann, Ralph A. Neumüller, Nicola Elvassore, Eric Siggia, James Briscoe, Anna Kicheva, Elly M. Tanaak

| Morphogenesis & mechanics

Overburdened Ferroptotic Stress Impairs Tooth Morphogenesis

Haisheng Wang, Xiaofeng Wang, Chenglin Wang, Fanyuan Yu, Ling Ye

Tooth morphogenesis from Wang et al. This image is made available under a CC-BY 4.0 International license.

The mechanical force with the physiological movement works as a biological cue in tendon development during the postnatal phase in mice

Yuna Usami, Takanori Kokubun

Viscous shear is a key force in Drosophila ventral furrow morphogenesis

Amanda Nicole Goldner, Mohamad Ibrahim Cheikh, Miriam Osterfield, Konstantin Doubrovinski

Coupling and uncoupling of midline morphogenesis and cell flow in amniote gastrulation

Rieko Asai, Vivek N. Prakash, Shubham Sinha, Manu Prakash, Takashi Mikawa

Single-cell lineage tracing reveals hierarchy and mechanism of adipocyte precursor maturation

Guillermo C. Rivera-Gonzalez, Emily G. Butka, Carolynn E. Gonzalez, Wenjun Kong, Kunal Jindal, Samantha A. Morris

Coordination between ECM and cell-cell adhesion regulates the development of islet aggregation, architecture, and functional maturation

Wilma Tixi, Maricela Maldonado, Ya-Ting Chang, Amy Chiu, Wilson Yeung, Nazia Parveen, Michael Nelson, Ryan Hart, Shihao Wang, Wu Jih Hsu, Patrick Fueger, Janel L. Kopp, Mark O. Huising, Sangeeta Dhawan, Hung-Ping Shih

A multi-tiered mechanical mechanism shapes the early neural plate

Angus Inman, Judith E. Lutton, Elisabeth Spiritosanto, Masazumi Tada, Till Bretschneider, Pierre A. Haas, Michael Smutny

A cytokinetic ring-driven cell rotation achieves Hertwig’s rule in early development

Teije C. Middelkoop, Jonas Neipel, Caitlin E. Cornell, Ronald Naumann, Lokesh G. Pimpale, Frank Jülicher, Stephan W. Grill

Exposure to the aryl hydrocarbon receptor agonist dioxin disrupts formation of the muscle, nerves, and vasculature in the developing jaw

Layra G. Cintrón-Rivera, Nicole Burns, Ratna Patel, Jessica Plavicki

| Genes & genomes

eIF4E1b is a non-canonical eIF4E required for maternal mRNA dormancy

Laura Lorenzo-Orts, Marcus Strobl, Benjamin Steinmetz, Friederike Leesch, Carina Pribitzer, Michael Schutzbier, Gerhard Duernberger, Andrea Pauli

Zebrafish ovaries from Lorenzo-Orts et al. This image is made available under a CC-BY-NC 4.0 International license.

Expression and molecular regulation of key genes during adipogenesis of neural crest stem cells

Bo-wen Dong, Wen-chao Guan, Kai Zhang, Yan Zhang, Yu Yang, Yan-ping Zhao, Rui Bai, Ming-xue Zheng, Xiao-zhen Cui

MYC is essential for induction of major ZGA and subsequent preimplantation development

Takuto Yamamoto, Haoxue Wang, Hana Sato, Shinnosuke Honda, Shuntaro Ikeda, Naojiro Minami

Thymic epithelial cell fate and potency in early organogenesis assessed by single cell transcriptional and functional analysis

An Chengrui, Alison M. Farley, Sam Palmer, Dong Liu, Anastasia I. Kousa, Paul Rouse, Viktoria Major, Joanna Sweetman, Jan Morys, Andrea Corsinotti, Jennifer Nichols, Jan Ure, Renee McLay, Luke Boulter, S. Jon Chapman, Simon R. Tomlinson, C. Clare Blackburn

High-throughput mRNA-seq atlas of human placenta shows vast transcriptome remodeling from first to third trimester

Tania L Gonzalez, Sahar Wertheimer, Amy E Flowers, Yizhou Wang, Chintda Santiskulvong, Ekaterina L Clark, Caroline A Jefferies, Kate Lawrenson, Jessica L Chan, Nikhil V Joshi, Yazhen Zhu, Hsian-Rong Tseng, S. Ananth Karumanchi, John Williams III, Margareta D Pisarska

EBF1 limits the numbers of cochlear hair and supporting cells and forms the scala tympani and spiral limbus during inner ear development

Hiroki Kagoshima, Hiroe Ohnishi, Ryosuke Yamamoto, Akiyoshi Yasumoto, Yosuke Tona, Takayuki Nakagawa, Koichi Omori, Norio Yamamoto

A genetic program for vascular pericyte precursors

Sarah J Childs, Suchit Ahuja, Cynthia Adjekukor, Qing Li, Katrinka M Kocha, Nicole Rosin, Elodie Labit, Sarthak Sinha, Ankita Narang, Quan Long, Jeff Biernaskie, Peng Huang

GATA-regulated transcriptional program dictate cell fate equilibrium to establish the maternal-fetal exchange interface and fetal development

Anany Ghosh, Rajnish Kumar, Ram P. Kumar, Soma Ray, Abhik Saha, Namrata Roy, Purbasa Dasgupta, Courtney Marsh, Soumen Paul

Maternal regulation of the vertebrate oocyte-to-egg transition

Ricardo Fuentes, Florence Marlow, Elliott Abrams, Hong Zhang, Manami Kobayashi, TriptiGupta, Lee Kapp, Zachary DiNardo, Felipe Montecinos-Franjola, William Vought, Charles Vejnar, Antonio E Giraldez, Mary C Mullins

DNA conserved across animals that controls genes for embryonic development

Martin C. Frith, Shengliang Ni

TFAP2 paralogs regulate midfacial development in part through a conserved ALX genetic pathway

Timothy T Nguyen, Jennyfer M Mitchell, Michaela D Kiel, Kenneth L Jones, Trevor J Williams, James T Nichols, Eric Van Otterloo

Single-cell transcriptional landscapes of bovine peri-implantation development

Giovanna Nascimento Scatolin, Hao Ming, Yinjuan Wang, Linkai Zhu, Emilio Gutierrez Castillo, Kenneth Bondioli, Zongliang Jiang

Loss Of function of Male-Specific Lethal 3 (Msl3) Does Not Affect Spermatogenesis in Rodents

T.A. Mitchell, J.M. Lin, S.M. Hicks, J.R. James, P. Rangan, P.E. Forni

Cell Type- and Tissue-specific Enhancers in Craniofacial Development

Sudha Sunil Rajderkar, Kitt Paraiso, Maria Luisa Amaral, Michael Kosicki, Laura E. Cook, Fabrice Darbellay, Cailyn H. Spurrell, Marco Osterwalder, Yiwen Zhu, Han Wu, Sarah Yasmeen Afzal, Matthew J. Blow, Guy Kelman, Iros Barozzi, Yoko Fukuda-Yuzawa, Jennifer A. Akiyama, Veena Afzal, Stella Tran, Ingrid Plajzer-Frick, Catherine S. Novak, Momoe Kato, Riana D. Hunter, Kianna von Maydell, Allen Wang, Lin Lin, Sebastian Preissl, Steven Lisgo, Bing Ren, Diane E. Dickel, Len A. Pennacchio, Axel Visel

Combinatorial transcription factor binding encodes cis-regulatory wiring of forebrain GABAergic neurogenesis

Rinaldo Catta-Preta, Susan Lindtner, Athena Ypsilanti, James Price, Armen Abnousi, Linda Su-Feher, Yurong Wang, Ivan Juric, Ian R. Jones, Jennifer A. Akiyama, Ming Hu, Yin Shen, Axel Visel, Len A. Pennacchio, Diane Dickel, John L R Rubenstein, Alex S Nord

Transcriptomic profiling of murine GnRH neurons reveals developmental trajectories linked to human reproduction

Yassine Zouaghi, Daniel Alpern, Vincent Gardeux, Julie Russeil, Bart Deplancke, Federico Santoni, Nelly Pitteloud, Andrea Messina

Transcriptomic profiling of tissue environments critical for post-embryonic patterning and morphogenesis of zebrafish skin

Andrew J. Aman, Lauren M. Saunders, August A. Carr, Sanjay R. Srivatsan, Colten D. Eberhard, Blake Carrington, Dawn Watkins-Chow, William J. Pavan, Cole Trapnell, David M. Parichy

Maternal regulation of the vertebrate egg-to-embryo transition

Ricardo Fuentes, Florence L. Marlow, Elliott W. Abrams, Hong Zhang, Manami Kobayashi, Tripti Gupta, Lee D. Kapp, Zachary DiNardo, Ronald Heller, Ruth Cisternas, Felipe Montecinos-Franjola, William Vought, Mary C. Mullins

| Stem cells, regeneration & disease modelling

Antero-posterior gradients of cell plasticity and proliferation modulate posterior regeneration in the annelid Platynereis

Loic Bideau, Loeiza Baduel, Marianne Basso, Pascale Gilardi-Hebenstreit, Vanessa Ribes, Michel Vervoort, Eve Gazave

Incomplete Reprogramming of DNA Replication Timing in Induced Pluripotent Stem Cells

Matthew Micheal Edwards, Ning Wang, Dashiell Massey, Dieter Egli, Amnon Koren

Derivation of NT-ESCs, iPSCs, and ESCs from Edwards et al. This image is made available under a CC-BY-NC 4.0 International license.

Identification of signalling pathways involved in gill regeneration in zebrafish

Laura Cadiz, Maddison Reed, Simon Monis, Marie-Andree Akimenko, Michael G. Jonz

Setdb1 safeguards genome integrity in muscle stem cells to allow for regenerative myogenesis and inflammation

Pauline Garcia, William Jarassier, Caroline Brun, Lorenzo Giordani, Cécile Peccate, Fany Agostini, Wai Hing Kung, Tom H Cheung, Fabien Le Grand

Autonomous and non-cell autonomous etiology of ciliopathy associated structural birth defects

Richard Francis, Jovenal T San Agustin, Heather L. Szabo Rogers, Cheng Cui, Julie A. Jonassen, Thibaut Eguether, John A. Follit, Cecilia W. Lo, Gregory J. Pazour

DNA damage primes hematopoietic stem cells for direct megakaryopoiesis

Corey M. Garyn, Oriol Bover, John W. Murray, Ma Jing, Karen Salas-Briceno, Susan R Ross, Hans-Willem Snoeck

Mannose is crucial for mesoderm specification and symmetry breaking in gastruloids

Chaitanya Dingare, Jenny Yang, Ben Steventon

Negative cell cycle regulation by Calcineurin is necessary for proper beta cell regeneration in zebrafish

Laura Massoz, David Bergemann, Arnaud Lavergne, Célia Reynders, Caroline Désiront, Chiara Goossens, Lydie Flasse, Bernard Peers, Marianne L. Voz, Isabelle Manfroid

Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin

N Cudak, AC López-Delgado, F Rost, T Kurth, M Lesche, S Reinhardt, A Dahl, S Rulands, F Knopf

Macrophages modulate fibrosis during newt lens regeneration

Georgios Tsissios, Anthony Sallese, J. Raul Perez-Estrada, Jared A. Tangeman, Weihao Chen, Byran Smucker, Sophia C. Ratvasky, Erika Grajales-Esquivel, Arielle Martinez, Kimberly J. Visser, Alberto Joven Araus, Hui Wang, Andras Simon, Maximina H. Yun, Katia Del Rio-Tsonis

 Newt eye at 4 days post-lentectomy from Tsissios et al. This image is made available under a CC-BY-NC 4.0 International license.

Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells

Liang-Yu Pang, Steven DeLuca, Haolong Zhu, John M. Urban, Allan C. Spradling

Differential roles of diet on development and spinal cord regeneration in larval zebrafish

Emily J Puriofy, Karen Mruk

Effect of a retinoic acid analogue on BMP-driven pluripotent stem cell chondrogenesis

Fabrizio E. Mancini, Paul Evelyn Alexander Humphreys, Steven Woods, Nicola Bates, Sara Cuvertino, Julieta O’Flaherty, Leela Biant, Marco A.N. Domingos, Susan J. Kimber

Compensatory growth and recovery of tissue cytoarchitecture after transient cartilage-specific cell death in foetal mouse limbs

Chee Ho H’ng, Shanika L. Amarasinghe, Boya Zhang, Hojin Chang, David Powell, Alberto Rosello-Diez

Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids

Zongyong Ai, Baohua Niu, Yu Yin, Lifeng Xiang, Gaohui Shi, Kui Duan, Sile Wang, Yingjie Hu, Chi Zhang, Chengting Zhang, Lujuan Rong, Ruize Kong, Tingwei Chen, Yixin Guo, Wanlu Liu, Nan Li, Shumei Zhao, Xiaoqing Zhu, Xuancheng Mai, Yonggang Li, Ze Wu, Yi Zheng, Jianping Fu, Weizhi Ji, Tianqing Li

Modelling Human Post-Implantation Development via Extra-Embryonic Niche Engineering

Joshua Hislop, Amir Alavi, Qi Song, Rayna Schoenberger, Kamyar Keshavarz F., Ryan LeGraw, Jeremy Velazquez, Tahere Mokhtari, Mohammad Nasser Taheri, Matthew Rytel, Susana M Chuva de Sousa Lopes, Simon Watkins, Donna Stolz, Samira Kiani, Berna Sozen, Ziv Bar-Joseph, Mo R. Ebrahimkhani

Transgene directed induction of a stem cell-derived human embryo model

Bailey AT Weatherbee, Carlos W Gantner, Riza M Daza, Nobuhiko Hamazaki, Lisa K. Iwamoto-Stohl, Jay Shendure, Magdalena Zernicka-Goetz

Gastruloid-derived Primordial Germ Cell-like Cells (Gld-PGCLCs) develop dynamically within integrated tissues

Christopher B. Cooke, Christopher Barrington, Peter Baillie-Benson, Jennifer Nichols, Naomi Moris

Ground-state pluripotent stem cells are characterized by Rac1-dependent Cadherin-enriched F-actin protrusions

Shiying Liu, Yue Meng, Pakorn Kanchanawong

Transgene-Free Ex Utero Derivation of A Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs

Bernardo Oldak, Emilie Wildschutz, Vladyslav Bondarenko, Alejandro Aguilera-Castrejon, Cheng Zhao, Shadi Tarazi, Mehmet-Yunus Comar, Shahd Ashouokhi, Dmitry Lokshtanov, Francesco Roncato, Sergey Viukov, Eitan Ariel, Max Rose, Nir Livnat, Tom Shani, Carine Joubran, Roni Cohen, Yoseph Addadi, Merav Kedmi, Hadas Keren-Shaul, Sophie Petropoulos, Fredrik Lanner, Noa Novershtern, Jacob H. Hanna

A miRNA screen identifies a transcriptional program controlling adult stem cell maintenance in organoids

Jacques Robert, Efstathios Vlachavas, Gilles Lemaître, Aristotelis Chatziioannou, Michel Puceat, Frederic Delom, Delphine Fessart

Discovery of Competent Chromatin Regions in Human Embryonic Stem Cells

Julian Pulecio, Zakieh Tayyebi, Dingyu Liu, Wilfred Wong, Renhe Luo, Jeyaram R Damodaran, Samuel Kaplan, Hyunwoo Cho, Jielin Yan, Dylan Murphy, Robert W. Rickert, Abhijit Shukla, Aaron Zhong, Federico González, Dexin Yang, Wenbo Li, Ting Zhou, Effie Apostolou, Christina S. Leslie, Danwei Huangfu

Single Cell Transcriptomics-Informed Induced Pluripotent Stem Cells Differentiation to Tenogenic Lineage

Angela Papalamprou, Victoria Yu, Wensen Jiang, Julia Sheyn, Tina Stefanovic, Angel Chen, Chloe Castaneda, Melissa Chavez, Dmitriy Sheyn

Liver-specification of human iPSC-derived endothelial cells transplanted into mouse liver

Kiryu K. Yap, Jan Schröder, Yi-Wen Gerrand, Anne M. Kong, Adrian M. Fox, Brett Knowles, Simon W. Banting, Andrew G. Elefanty, Eduoard G. Stanley, George C. Yeoh, Glen P. Lockwood, Victoria C. Cogger, Wayne A. Morrison, Jose M. Polo, Geraldine M. Mitchell

Canonical Wnt and TGF-β/BMP signaling enhance melanocyte regeneration and suppress invasiveness, migration, and proliferation of melanoma cells

Esra Katkat, Yeliz Demirci, Guillaume Heger, Doga Karagulle, Irene Papatheodorou, Alvis Brazma, Gunes Ozhan

Establishment of a novel non-integrated human pluripotent stem cell-based gastruloid model

Gege Yuan, Jiachen Wang, Zhaode Liu, Mengqi Chen, Pinmou Zhu, Hao Zhang, Zhibin Hu, Yiqiang Cui, Yan Yuan, Jiahao Sha

Regeneration leads to global tissue rejuvenation in aging sexual planarians

Xiaoting Dai, Xinghua Li, Scott Pletcher, David Paris, Leonid Kruglyak, Jacob Sobota, Longhua Guo

Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells

Liang-Yu Pang, Steven DeLuca, Haolong Zhu, John M. Urban, Allan C. Spradling

SRSF1-mediated alternative splicing of Tial1/Tiar is essential for homing and self-renewal in mouse spermatogonial stem cells

Longjie Sun, Zheng Lv, Xuexue Chen, Rong Ye, Shuang Tian, Chaofan Wang, Xiaomei Xie, Lu Yan, Xiaohong Yao, Yujing Shao, Sheng Cui, Juan Chen, Jiali Liu

| Plant development

Harnessing Deep Learning to Analyze Cryptic Morphological Variability of Marchantia polymorpha

Yoko Tomizawa, Naoki Minamino, Eita Shimokawa, Shogo Kawamura, Aino Komatsu, Takuma Hiwatashi, Ryuichi Nishihama, Takashi Ueda, Takayuki Kohchi, Yohei Kondo

Regulation of adaptive growth decisions via phosphorylation of the TRAPPII complex in Arabidopsis

Christian Wiese, Miriam Abele, Benjamin Al, Melina Altmann, Alexander Steiner, Nils Kalbfuss, Alexander Strohmayr, Raksha Ravikumar, Chan Ho Park, Barbara Brunschweiger, Chen Meng, Eva Facher, David W. Ehrhardt, Pascal Falter-Braun, Zhi-Yong Wang, Christina Ludwig, Farhah F. Assaad

Mapping the landscape of transcription factor promoter activity during vegetative development in Marchantia

Facundo Romani, Susanna Sauret-Güeto, Marius Rebmann, Davide Annese, Ignacy Bonter, Marta Tomaselli, Tom Dierschke, Mihails Delmans, Eftychios Frangedakis, Linda Silvestri, Jenna Rever, John L Bowman, Ignacio Romani, Jim Haseloff

Notch area in the Marchantia gemma from Romani et al. This image is made available under a CC-BY 4.0 International license.

ITPK1 regulates jasmonate-controlled root development in Arabidopsis thaliana

Naga Jyothi Pullagurla, Supritam Shome, Ranjana Yadav, Debabrata Laha

Comprehensive regulatory networks for tomato organ development based on the genome and RNAome of microTom tomato

Jia-Yu Xue, Hai-Yun Fan, Zhen Zeng, Yu-Han Zhou, Shuai-Ya Hu, Sai-Xi Li, Ying-Juan Cheng, Xiang-Ru Meng, Fei Chen, Zhu-Qing Shao, Yves Van de Peer

Single-cell transcriptomic analysis of pea shoot development and cell-type-specific responses to boron deficiency

Xi Chen, Yanqi Ru, Hirokazu Takahashi, Mikio Nakazono, Sergey Shabala, Steven M. Smith, Min Yu

Dynamics of the epigenetic landscape during development and in response to drought stress in sorghum

Yongfeng Hu, Chao He, Yuning Shen, Gongjian Zeng, Siteng Bi, Quanjun Huang, Xiner Qin, Zhuying Deng, Zhengquan He, Xiangling Shen

| Evo-devo

Evolution of the gene regulatory network of body axis by enhancer hijacking in amphioxus

Chenggang Shi, Shuang Chen, Huimin Liu, Shiqi Li, Yanhui Wang, Rongrong Pan, Xiaotong Wu, Xuewen Li, Jingjing Li, Chaofan Xing, Xian Liu, Yiquan Wang, Qingming Qu, Guang Li

Different stages of B. floridae embryos from Shi et al. This image is made available under a CC-BY 4.0 International license.

Co-evolutionary dynamics of mammalian brain and body size

Chris Venditti, Joanna Baker, Robert A Barton

A fitness landscape instability governs the morphological diversity of tip-growing cells

Maxim E. Ohairwe, Branka D. Živanović, Enrique R. Rojas

Expanding the Evo-Devo Toolkit: Generation of 3D mammary tissue from diverse mammals

Hahyung Y. Kim, Ishani Sinha, Karen E. Sears, Charlotte Kuperwasser, Gat Rauner

Dissecting genetic correlation through recombinant perturbations: the role of developmental bias

Haoran Cai, Kerry Geiler-Samerotte, David L. Des Marais

Cell Biology

Spatially and temporally distinct patterns of expression for VPS10P domain receptors in human cerebral organoids

Fabia Febbraro, Helena Hørdum Breum Andersen, Thomas E. Willnow

Mechanisms of Pathogenicity of Hypertrophic Cardiomyopathy-Associated Troponin T (TNNT2) Variant R278C+/- During Development

Sanam Shafaattalab, Alison Y Li, Farah Jayousi, Yasaman Maaref, Homa Hamledari, Dina Hosseini Baygi, Tiffany Barszczewski, Marvin Gunawan, Balwinder Ruprai, Shayan Jannati, Raghu Nagalingam, Austin M Cool, Paulina Langa, R John Solaro, Shubhayan Sanatani, Christopher Toepfer, Steffen Lindert, Philipp Lange, Glen F Tibbits

Epsilon tubulin is an essential determinant of microtubule-based structures in male germ cells

G. Gemma Stathatos, D. Jo Merriner, Anne E. O’Connor, Jennifer Zenker, Jessica E.M. Dunleavy, Moira K. O’Bryan

The proprotein convertase BLI-4 promotes collagen secretion during assembly of the Caenorhabditis elegans cuticle

Susanna K. Birnbaum, Jennifer D. Cohen, Alexandra Belfi, John I. Murray, Jennifer R.G. Adams, Andrew D. Chisholm, Meera V. Sundaram

Endodermal cells use contact inhibition of locomotion to achieve uniform cell dispersal during zebrafish gastrulation

Jesselynn LaBelle, Tom Wyatt, Stephanie Woo

Zebrafish endodermal cells from LaBelle et al. This image is made available under a CC-BY-NC-ND 4.0 International license.

Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill Required for Fertilization

Martina Jablonski, Guillermina M Luque, Matias Gomez-Elias, Claudia Sanchez-Cardenas, Xinran Xu, Jose L de la Vega-Beltran, Gabriel Corkidi, Alejandro Linares, Victor Abonza, Dario Krapf, Diego Krapf, Alberto Darszon, Adan Guerrero, Mariano G Buffone

The ultrastructural nature of human oocytes’ cytoplasmatic abnormalities and the role of cytoskeleton dysfunction

Martina Tatíčková, Zuzana Trebichalská, Drahomíra Kyjovská, Pavel Otevřel, Soňa Kloudová, Zuzana Holubcová

Disruption of maternal ACKR3 has profound effects on embryos and offspring

Ayumi Fukuoka, Gillian J. Wilson, Elise Pitmon, Lily Koumbas Foley, Hanna Johnsson, Marieke Pingen, Gerard J. Graham

Embryonic origins of bipotent endothelial-macrophage progenitor cells in postnatal aorta

Anna E. Williamson, Sanuri Liyanage, Mohammadhossein Hassanshahi, Malathi S.I. Dona, Deborah Toledo-Flores, Dang X.A. Tran, Catherine Dimasi, Nisha Schwarz, Sanuja Fernando, Thalia Salagaras, Aaron Long, Jan Kazenwadel, Natasha L. Harvey, Grant R. Drummond, Antony Vinh, Vashe Chandrakanthan, Ashish Misra, Zoltan Neufeld, Joanne T.M. Tan, Luciano Martelotto, Jose M. Polo, Claudine S. Bonder, Alexander R. Pinto, Shiwani Sharma, Stephen J. Nicholls, Christina A. Bursill, Peter J. Psaltis

Spatiotemporal cell landscape of human embryonic tooth development

Yueqi Shi, Yejia Yu, Jutang Li, Shoufu Sun, Li Han, Shaoyi Wang, Ke Guo, Jingang Yang, Wenjia Wei, Jin Qiu

Role of BicDR in bristle shaft construction, tracheal development, and support of BicD functions

Aleksandra Jejina, Yeniffer Ayala, Greco Hernández, Beat Suter

Wg/Wnt-signaling induced nuclear translocation of β-catenin is attenuated by a β-catenin peptide through its interaction with IFT-A in development and cancer cells

Linh T. Vuong, Marek Mlodzik

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem cells

Emily Osterli, Mary Ellenbecker, Xiaobo Wang, Mikaya Terzo, Ketch Jacobson, DeAnna Cuello, Ekaterina Voronina

Mutant analysis of Kcng4b reveals how the different functional states of the voltage-gated potassium channel regulate ear development

Justyna Jędrychowska, Vitya Vardanyan, Milosz Wieczor, Antoni Marciniak, Jacek Czub, Razieh Amini, Ruchi Jain, Hongyuan Shen, Hyungwon Choi, Jacek Kuznicki, Vladimir Korzh

Modelling

Deep learning pipeline reveals key moments in human embryonic development predictive of live birth in IVF

Camilla Mapstone, Helen Hunter, Daniel Brison, Julia Handl, Berenika Plusa

Cell jamming and unjamming in development: physical aspects

Ivana Pajic-Lijakovic, Milan Milivojevic

Modeling soybean growth: A mixed model approach

Maud Delattre, Yusuke Toda, Jessica Tressou, Hiroyoshi Iwata

The Core & Periphery Hypothesis: A Conceptual Basis for Generality in Cell and Developmental Biology

Elisa Gallo, Stefano De Renzis, James Sharpe, Roberto Mayor, Jonas Hartmann

Tools & Resources

Accurate staging of chick embryonic tissues via deep learning

Ian Groves, Jacob Holmshaw, David Furley, Matthew Towers, Benjamin D. Evans, Marysia Placzek, Alexander G. Fletcher

Morphological changes in the chick embryo brain from Groves et al. This image is made available under a CC-BY 4.0 International license.

But, what are the cells doing? Image Analysis pipeline to follow single cells in the zebrafish embryo

Arianne Bercowsky-Rama, Olivier F. Venzin, Laurel A. Rohde, Nicolas Chiaruttini, Andrew C. Oates

Zebrahub – Multimodal Zebrafish Developmental Atlas Reveals the State-Transition Dynamics of Late-Vertebrate Pluripotent Axial Progenitors

Merlin Lange, Alejandro Granados, Shruthi VijayKumar, Jordão Bragantini, Sarah Ancheta, Sreejith Santhosh, Michael Borja, Hirofumi Kobayashi, Erin McGeever, Ahmet Can Solak, Bin Yang, Xiang Zhao, Yang Liu, Angela M. Detweiler, Sheryl Paul, Honey Mekonen, Tiger Lao, Rachel Banks, Yang-Joon Kim, Adrian Jacobo, Keir Balla, Kyle Awayan, Samuel D’Souza, Robert Haase, Alexandre Dizeux, Olivier Pourquie, Rafael Gómez-Sjöberg, Greg Huber, Mattia Serra, Norma Neff, Angela Oliveira Pisco, Loïc A. Royer

Zebrahub’s scRNAseq experimental pipeline from Lange et al. This image is made available under a CC-BY-NC-ND 4.0 International license.

A Highly Efficient Aorta-Gonad-Mesonephros-Like Definitive Hemogenic Endothelium From Human Pluripotent Stem Cells

Yesai Fstkchyan, Qingwen Cheng, Jingli Zhang, Daniel Lu, Guanyi Huang, Tiange Dong, Lauren Jones, Matt Kanke, Chris Hale, Kristin Tarbell, Chi-Ming Li, Songli Wang, Stuart M. Chambers

Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

Mathieu C. Husser, Nhat P. Pham, Chris Law, Flavia R. B. Araujo, Vincent J.J. Martin, Alisa Piekny

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Avian Model Systems 11 deadline extended to July 21st

Posted by , on 4 July 2023

The abstract submission deadline for the 11th Avian Model Systems meeting, 11-14 September 2023 in Portsmouth, UK, as been extended to 21 July 2023.

The conference will for the first time since COVID-19 bring together the research community using avian models in research. We have an exciting line-up of top international speakers covering topics like stem cells, developmental biology, new technologies, immunology, comparative genomics and evolution, and the use of avian models for medical research. Besides the keynote speakers, the programme features short and flash talks selected from the abstracts, poster sessions, a plenary discussion on avian models in research, and a PI meeting to discuss the development of resources for the international avian research community.

Outside the conference Portsmouth and the South coast of England provide a range of attractive sights to visit. Portsmouth can easily be reached by train and from London Heathrow and Gatwick airports and Southampton airport. It also has ferry links with the Channel Islands, France and Spain.

We are very much looking forward to welcoming the avian model research community in Portsmouth. Please spread the word!

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Categories: Events

Lab meeting with the Excellent Unit of Wildlife Stem Cells

Posted by , on 4 July 2023

Lab website : https://ewiconvsthailand.tech

Where is the lab?

Excellent Unit of Wildlife Stem Cells (eWIS) is housed in two remarkable locations: Wildlife Reproductive Innovation Center (WRIC) at Khao Kheow Open Zoo (as part of Zoological Part Organization of Thailand) and Department of Biology, Faculty of Science, Burapha University. Both reside in Chonburi Province, Thailand.

Research summary

The eWIS initiative brings together a team of stem cell scientists and veterinarians, forging connections between universities and zoos. The primary objective is to explore the immense potential of stem cell technology in establishing a biobank comprised of stem cells derived from endangered mammalian and avian species. Our research focuses on species that require urgent preservation efforts to safeguard genetic diversity through the collection of viable cells capable of generating entire animal bodies, known as induced pluripotent stem cells (iPSCs). Additionally, we are striving to generate wild felid/ domestic and non-domestic cat embryonic stem cells.

Our primary focus lies in wild felid species, including fishing cat (Prionailurus viverrinus) and clouded leopard (Neofelis nebulosa), and domestic cat (Felis catus) as a model. Our goal is to generate iPSCs from these wild cats, unravelling the possibilities of utilizing iPSCs for conservation purposes through in vitro gametogenesis, animal cloning, and assisted reproductive technology (ART). We are also devoted to establishing wild avian iPSCs, with specific attention given to hornbills and peafowl.

Furthermore, we employ a range of evolutionary tools to delve into the conservation and divergence of pluripotency networks across various vertebrate species. In particular, our research involves studying Oct4 homologues to comprehend the effects of evolutionary changes on conserved and diverged Oct4 functions. The profound insights gained from understanding the Oct4 network and early developmental programs contribute to translational approaches, aimed at overcoming reprogramming barriers in wild species.

The eWIS research team primarily works within one of the largest zoos in the world, “Khao Kheow Open Zoo,” and Burapha University in Chonburi, Thailand. From left to right: Pawares, Chichakon, Pitiwut, Woranop, Ampika, Rattanaporn, Santhita, Nattakorn. (Photo credit : Nattawut Dueanjam).

Lab roll call

Santhita: Researcher – Managing routine lab works/paper works at WRIC, bridging zoo units to collect wild animal tissues (mostly dead) to eWIS; Organizing biobank at the zoo

Pathira : Lab Manager/Researcher – Managing routine lab works/paper works/ordering at eWIS lab at Department of Biology; Generating barnacle collection

Pawares: Pre-doc student – searching cat ovaries and testes around the city!, IVF of cat embryos, cat pluripotency explorer

Nattakorn: Master student – primary culture of tissues from live and dead mammals and birds; RNA-based reprogramming; bioinformatics on pluripotency network

Ratchapon: Master student – Wild avian/chicken reprogramming and understanding the network within avian pluripotency

Pitiwut & Rattanaporn & Phanpimon: BSc students – characterization of felid pluripotency and cat IVF/IVM

Chichakon & Phornchanok: BSc students – avian reprogramming: testing various sets of reprogramming factors

Purachet : BSc student – Sea urchin hunter ! and barnacle biology

Ampika: PI – advice the team, developing ART research, searching research grant, building capacity of the team and strengthen research network

Woranop: PI – teaching, admin jobs, pipetting, being Head (meeting job) & Biology program director, making connections between organizations

Favourite technique, and why

Woranop: Cellular reprogramming is my favourite tool. It is fascinating of how these felid cells can uptake reprogramming factors and transform the morphology and reverse cell fate! I love immunostaining of pre-implantation embryos from IVF- this is stunning how a single cell can become diversified in forms and functions! Recently, I discover myself to enjoy working on structural models predicted from AlphaFold2 which helps understand the evolution of proteins orchestrating the development.

Ampika: ARTs including AI, IVF and cloning are my favourite techniques for enhancing breeding conservation program of important species such as fishing cat, clouded leopard, flat-headed cat (Prionailurus planiceps), Asian golden cat (Catopuma temminckii), Eld’s deer (Rucervus eldii) and Asian elephant (Elephas maximus). Developing cryopreservation for establishment of “Viable Cell Bank” is crucial as it is important as insurance against loss or extinct of endangered species. These techniques are valuable tools that we use to sustain the genetic diversity of our endemic and global important species.

Fishing cat, one of the wild felid species, is currently in a vulnerable status due to wetland destruction. The Zoological Park Organization of Thailand (ZPOT) prioritizes research efforts with universities to save fishing cats via assisted reproductive technology, particularly artificial insemination. We are utilizing stem cell technology to establish a biobank and perform cellular reprogramming in the Excellent Unit of Wildlife Stem Cells (eWIS). (Photo credit: Piyapong Chinnadate)

Apart from your own research, what are you most excited about in developmental and stem cell biology?

Woranop: It is amazing how cells know how to reorganize themselves into specific shapes and transform into particular lineages. The technology to generate synthetic embryos, based on our understanding of cell potency and differentiation, is incredibly exciting. I am curious to see how this technology will merge with our understanding of wild stem cells and eventually contribute to saving wild animals.

Ampika: It is very exciting to see how we can develop stable stem cell colonies and differentiate them into various cell types that can be applied for medical treatments and reproductive purposes. This capability is incredibly useful for sustaining wildlife populations in the future!

How do you approach managing your group and all the different tasks required in your job?

Woranop: Everything is all about team work and communication! Regular meet-up helps a lot. We also frequently hang out for lunch or dinner, creating a comfortable environment for students to freely talk and share ideas.

Ampika: Update and discuss in anytime and set up a small meeting at least once a month.

What is the best thing about where you work?

Woranop : I enjoy working multidisciplinary fields of biology at our department and visiting the zoo every time I go to the eWIS lab. It allows me to appreciate animals and nature.

Ampika : The best part of our work is that we apply all our knowledge to prevent our wildlife from facing the brink of extinction! Our work supports not only ex situ management but also in situ conservation.

Santhita: At the zoo, we have access to a diverse range of wild animal species, which enables us to quickly collect samples for our work after the animals die or during health checks.

Pathira: The department admin staffs – we can ask for almost everything from lab materials to animal/plant specimens. 

Pawares: Working with developing embryos often fills my heart with boundless excitement.

Nattakorn: I love being a TA and teaching undergraduate students! It provides me with opportunities to help other young students develop a passion for science through activities like science camps. It also allows me to support them in navigating university courses and life.

Ratchapon: The teachers in our department are very friendly, and we can contact them to get advice anytime.

Pitiwut: Everyone here is a bundle of joy- we can engage in conversations about anything, no matter how unrelated to work it may be!

Rattanaporn: Learning new things all the time

Phanpimon: Our advisors are the best! They always provide great support.

Chichakon: There are plenty of opportunities to engage in biology research starting from my third year! I love the open exchange of ideas we have in the lab.

Phornchanok: The great friendships in the lab make everything easier.

Purachet: Enjoy Sukiyaki parties with admin staffs in the department lunch breaks!

eWIS research team

What is there to do outside of the lab?

Woranop : I teach undergraduate and graduate students, and it brings me joy to share the fascinating stories of developmental biology with them outside the lab. We are also located near Bangsaen Beach! I often hang out with our team and visit other beaches along the eastern coast of Thailand.

Ampika : Since we work with 6 zoos nationwide and the Elephant Kingdom Project, it is very enjoyable to explore the places and talk to local people. And of course, Bangsaen is my favourite place to R&R!

Santhita: Sometimes in the evening, we take walks around the zoo to be close to nature. Nearby, there are tourist attractions such as Chan Ta Then waterfall and Bang Pra reservoir.

Pathira: Hangout at the zoo, Capybara watching! & definitely, Bangsaen is a right place for seafood, in particular grilled shrimp/squid

Pawares: I help organize department events and take care of our small colonies of chickens!

Nattakorn: I enjoy playing online games while waiting for my phylogenetic analyses! The beautiful Bangsaen beach also provides great help during exam or lab meeting crises

Ratchapon: I assist the department’s administrative staffs for specific events and serve as a TA to help Woranop teach the Developmental Biology Lab.

Pitiwut: I keep myself fit and healthy by doing cardio exercises at the nearby gym! I think it helps clear my thoughts a lot after a long day of studying and working in the lab.

Rattanaporn: Moo-gata (Traditional Thai BBQ!) is a must here.

Phanpimon: Jet skiing, running on the beach, or even feeding the monkeys on Khao Sam Muk hill.

Chichakon: I love designing art works for my lab and also others in the department.

Phornchanok: We can do a lot of things in Bangsaen – shopping, visiting cafes, having dinner, and enjoying the beach!

Purachet: Outside of class and the lab, I spend most of my time in the department studio, where I assist with photography and collect biological samples for departmental teaching.

Browse through other ‘Lab meeting’ posts featuring developmental and stem cell biology labs around the world.

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Join us on biologists.social

Posted by , on 29 June 2023

The Company of Biologists has launched a new server on Mastodon: biologists.social. It is a space for biologists to discuss science, research, teaching, life and more.

You can find the Node on this server, along with FocalPlane, preLights, and our peer-reviewed journals Development and others.

We invite you to join us on this server to build a community for biologists and to engage in lively discussions.

You can read the full story on why we decided to launch our own Mastodon server and some tips on how to join us on biologists.social.

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Genetics Unzipped: S6.13: Kings, car parks and consent: should we sequence DNA from human remains?

Posted by , on 29 June 2023

Richard III was King of England from 1483 to 1485. There are some contemporary records. One talks about him having one shoulder higher than the other. So when we were excavating, we thought maybe we’ll find somebody with a spinal abnormality.

Prof. Turi King

In the latest episode of the Genetics Unzipped podcast, we’re venturing into ancient territory and archaeological digs, excavating the complex ethics of extracting and sequencing DNA from human remains. We chat to Prof. Turi King about her role uncovering the body King Richard III and who decides which research gets done.

Genetics Unzipped is the podcast from The Genetics Society. Full transcript, links and references available online at GeneticsUnzipped.com.

Subscribe from Apple podcasts, Spotify, or wherever you get your podcasts.

Head over to GeneticsUnzipped.com to catch up on our extensive back catalogue.If you enjoy the show, please do rate and review on Apple podcasts and help to spread the word on social media. And you can always send feedback and suggestions for future episodes and guests to podcast@geneticsunzipped.com Follow us on Twitter – @geneticsunzip

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Development presents… June webinar on growth and morphogenesis

Posted by , on 28 June 2023

On 21 June 2023, Development hosted a webinar featuring talks on the topic of growth and morphogenesis from three early career researchers, chaired by Development’s Editor-in-Chief, James Briscoe. Below are the recordings of the talks.

Patricio Pérez-Henríquez (University of California, Riverside)

Talk and Q&A by Patricio Pérez-Henríquez

Stefania Tavano (MedUni Vienna)

Talk and Q&A by Stefania Tavano

Stefan Harmansa (IBDM and Turing Center for Living Systems)

Talk and Q&A by Stefan Harmansa
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Categories: Development presents..., Video